期刊
ARCHIVES OF VIROLOGY
卷 160, 期 7, 页码 1781-1784出版社
SPRINGER WIEN
DOI: 10.1007/s00705-015-2441-z
关键词
Bufavirus; Diarrhea; NS1 gene; Phylogenetic analysis
类别
资金
- Ratchadaphisek Somphot Fund
- National Research Council of Thailand
- Higher Education Research Promotion and National Research University Project of Thailand Office of the Higher Education Commission [WCU-58-006-HR, WCU007-HR57, WCU 001-HR57]
- Center of Excellence in Clinical Virology, Chulalongkorn University
- Chulalongkorn University Centenary Academic Development Project [CU56-HR01]
- Ratchadaphiseksomphot Endowment Fund of Chulalongkorn University [RES560530093]
- Outstanding Professor of the Thailand Research Fund [DPG5480002]
- National Science and Technology Development Agency
- MK restaurant Company Limited
- Siam Cement Group PcL
- King Chulalongkorn Memorial Hospital
Bufavirus (BuV) was initially discovered in fecal samples from children with acute diarrhea. In this study, we determined the prevalence, distribution, and genotype(s) of BuV in Thailand. A total of 1,495 diarrheal and 741 non-diarrheal stool specimens were collected and analyzed. A portion of the NS1 gene of BuV was amplified by nested RT-PCR. Phylogenetic analysis was performed to classify the BuV strains found. We detected bufavirus (BuV) in diarrheal (4/1495; 0.27 %) but not in non-diarrheal specimens (0/726). All four strains belonged to BuV genotype 1. BuV could be detected in adults and children, but its role in causing acute diarrhea remains unclear.
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