Article
Biochemical Research Methods
Jiechen Shen, Li Jia, Liuyi Dang, Yuanjie Su, Jie Zhang, Yintai Xu, Bojing Zhu, Zexuan Chen, Jingyu Wu, Rongxia Lan, Zhifang Hao, Chen Ma, Ting Zhao, Ni Gao, Jieyun Bai, Yuan Zhi, Jun Li, Junying Zhang, Shisheng Sun
Summary: This study introduces a modularization strategy and an algorithm named StrucGP for automated interpretation of N-glycan structures using tandem mass spectrometry, enabling detailed glycan structure determination on thousands of glycosites. The approach aids in identifying rare/new glycan structures and facilitates in-depth structural and functional study of glycoproteins in the biomedical research field. StrucGP offers a novel de novo glycan mapping method for determining detailed N-glycan structures at the site-specific level.
Article
Biochemical Research Methods
Jiangming Huang, Shouzeng Hou, Jiao An, Chenliang Zhou
Summary: In this study, a full characterization of N-glycosylation was performed for a Chinese Hamster Ovary (CHO) cell expressed variant-designed spike protein. The study revealed 19 N-glycosites at intact N-glycopeptide level and identified 31 high-abundance N-glycans through quantitative analysis by LC-MS/MS. Sequencing analysis of glycans confirmed the glycan structure. The results showed good batch-to-batch consistency, providing valuable information for SARS-CoV-2 spike protein analysis and quality studies.
ANALYTICAL AND BIOANALYTICAL CHEMISTRY
(2023)
Article
Chemistry, Analytical
Luyao Liu, Bin Zhu, Zheng Fang, Na Zhang, Hongqiang Qin, Zhimou Guo, Xinmiao Liang, Zhenzhen Yao, Mingliang Ye
Summary: Bottom-up proteomics is increasingly used in clinical research, but glycoproteomic analysis has been a bottleneck. An automated glycopeptide enrichment method was developed for site-specific N-glycoproteome analysis, showing higher efficiency and reliability.
ANALYTICAL CHEMISTRY
(2021)
Review
Food Science & Technology
Boyuan Guan, Zhenghan Zhang, Yuxia Chai, Xiakouna Amantai, Xinping Chen, Xueyan Cao, Xiqing Yue
Summary: This article summarizes the synthetic pathways, types, and distribution characteristics of protein N-glycosylation in milk, exploring further research supported by mass spectrometry and bioinformatics algorithms. N-glycosylation of milk proteins plays a crucial role in dairy processing and metabolism, impacting intestinal microbiota, immune function, and brain development.
TRENDS IN FOOD SCIENCE & TECHNOLOGY
(2022)
Article
Chemistry, Analytical
Myung Jin Oh, Unyong Kim, Sol Kim, Dae Sik Cho, Jung-A Seo, Nari Seo, Joo An
Summary: Glycosylation plays a crucial role in the quality, stability, safety, immunogenicity, pharmacokinetics, and efficacy of biotherapeutics. Various methods have been used for glyco-characterization at different levels. Intact protein analysis is considered a rapid glycoform monitoring approach; however, it can be challenging for biotherapeutics with multiple glycosylation sites. A new strategy using two-step intact glycoform mass spectrometry has been developed to address this challenge, providing rapid and accurate information on the degree of glycosylation. This method can be used to assess glycosylation similarity between batches and between biosimilar and reference during development and production.
JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS
(2023)
Article
Immunology
Enyue Fang, Miao Li, Xiaohui Liu, Kongxin Hu, Lijuan Liu, Zelun Zhang, Xingxing Li, Qinhua Peng, Yuhua Li
Summary: A genetically stable attenuated dengue virus strain was successfully constructed by changing amino acid sequences in the NS1 and NS2A glycoproteins. The virus strain showed significantly reduced virulence in mice during neurovirulence assay. This study provides a theoretical basis for understanding the pathogenesis of dengue virus and developing live attenuated vaccines through the deletion of amino acid residues at the N-glycosylation site.
Review
Biochemical Research Methods
Elli Makrydaki, Pavlos Kotidis, Karen M. Polizzi, Cleo Kontoravdi
Summary: N-glycosylation is crucial for understanding human disease mechanisms and ensuring the safety and efficacy of biotherapeutics. Capillary electrophoresis is emerging as a versatile and adaptable method for glycan analysis due to its speed, sensitivity, and complementarity with standard techniques, making it increasingly used in academia and industry for glycomics and glycoproteomics.
CURRENT OPINION IN BIOTECHNOLOGY
(2021)
Article
Biochemistry & Molecular Biology
Chunqun Wang, Wenjie Gao, Shi Yan, Xing-Quan Zhu, Xun Suo, Xin Liu, Nishith Gupta, Min Hu
Summary: N-glycosylation is a physiologically vital post-translational modification in eukaryotic organisms, and Haemonchus contortus has exclusive chitobiose modifications in its N-glycans. Immunogenic proteins like peptidases are N-glycosylated in the parasite, with glycan-rich conjugates primarily located in the intestine and gonads of adult worms, evading host immune responses. This comprehensive atlas of N-glycosylation in the prevalent parasitic nematode highlights its importance for infection, immunity, and prevention.
COMPUTATIONAL AND STRUCTURAL BIOTECHNOLOGY JOURNAL
(2021)
Article
Chemistry, Analytical
Chia Yen Liew, Hong-Sheng Luo, Ting-Yi Yang, An-Ti Hung, Bryan John Abel Magoling, Charles Pin-Kuang Lai, Chi-Kung Ni
Summary: This study re-examines high mannose N-glycans in multicellular eukaryotes using a new mass spectrometry method, and identifies many previously unreported isomers. A database is constructed for rapid identification of high mannose N-glycan isomers.
ANALYTICAL CHEMISTRY
(2023)
Article
Biochemical Research Methods
Dinko Soic, Toma Keser, Jerko Stambuk, Domagoj Kifer, Flemming Pociot, Gordan Lauc, Grant Morahan, Mislav Novokmet, Olga Gornik
Summary: Recent studies have shown that children with early-onset type 1 diabetes (T1D) have a higher proportion of oligomannose glycans in their plasma protein N-glycome compared to healthy siblings. This study aimed to characterize the glycosylation profile of complement component C3 in T1D and found significant changes in C3 N-glycan profiles associated with T1D. The study also confirmed the involvement of C3 N-glycome in T1D development.
MOLECULAR & CELLULAR PROTEOMICS
(2022)
Article
Biochemistry & Molecular Biology
Akos Tiboldi, Johannes Fuehrer, Wolfgang Schaubmayr, Eva Hunyadi-Gulyas, Marie Louise Zach, Beatrix Hochreiter, Andreas Spittler, Roman Ullrich, Klaus Markstaller, Friedrich Altmann, Klaus Ulrich Klein, Verena Tretter
Summary: This study investigated the effects of different oxygen conditions on the glycobiology of the pulmonary endothelium, showing that oxygen can influence glycan structures which in turn affect cell interactions and signaling.
Article
Multidisciplinary Sciences
Zheng Fang, Hongqiang Qin, Jiawei Mao, Zhongyu Wang, Na Zhang, Yan Wang, Luyao Liu, Yongzhan Nie, Mingming Dong, Mingliang Ye
Summary: The traditional site-specific glycoproteomics analysis often fails to account for glycopeptides with unusual glycans or poor peptide backbone fragmentation. In this study, a glycoproteomics tool called Glyco-Decipher is developed to address these issues. Glyco-Decipher utilizes database-independent peptide matching and the fragmentation pattern of shared peptide backbones to improve spectrum interpretation. Benchmark tests demonstrate that Glyco-Decipher identifies more peptide-spectrum matches than other existing tools, resulting in a significant increase in the number of identified glycopeptide spectra. By enabling unbiased profiling of attached glycans, Glyco-Decipher enables the discovery of modified glycans in mouse tissues, including those with chemical or biological modifications. This tool holds great promise for advancing glycoproteomics analysis in biological research.
NATURE COMMUNICATIONS
(2022)
Article
Biochemistry & Molecular Biology
Mingming Xu, Hong Jin, Wei Ge, Lingbo Zhao, Zhaoliang Liu, Zeyu Guo, Zhen Wu, Jing Chen, Chengjie Mao, Xumin Zhang, Chun-Feng Liu, Shuang Yang
Summary: Urine contains a wide range of glycoproteins, peptides, and free glycans, which can provide earlier and more sensitive molecular changes for biomarker discovery. Urine biomarkers have great diagnostic potential and are easy to collect and analyze non-invasively. The investigation of abnormal protein glycosylation in urine may shed light on the identification of disease-specific glycosylation biomarkers for Parkinson's disease.
ACS CHEMICAL NEUROSCIENCE
(2023)
Article
Virology
L. Johanna Gebhard, Zlata Vershinin, Tomas Alarcon-Schumacher, Jerry Eichler, Susanne Erdmann
Summary: N-glycosylation is crucial for cell stability, motility, and virus-host interactions in Archaea. The study investigated the impact of compromised N-glycosylation on virus infection dynamics using a mutant strain of Halorubrum lacusprofundi. The results showed that compromised N-glycosylation had different effects on different viruses, highlighting the significance of N-glycosylation in virus-host interactions.
Article
Immunology
Dejian Liu, Xuyao Xiao, Peng Zhou, Huijun Zheng, Yaqian Li, Hui Jin, Anan Jongkaewwattana, Rui Luo
Summary: The study demonstrated the crucial roles of E glycosylation in replication and pathogenicity of Duck Tembusu virus (DTMUV) in ducks; Disruption of E glycosylation could substantially impair virus attachment, entry, and infectivity; The attenuated phenotypes of mutant viruses in vivo may be partly associated with lower inflammatory cytokines expression.
Article
Immunology
Guillaume Ravel, Nathalie Mantel, Jeremy Silvano, Alexandra Rogue, Bruno Guy, Nicholas Jackson, Nicolas Burdin
Article
Biochemical Research Methods
Cynthia Chapel, Nathalie Rocher-Helion, Nathalie Mantel, Sandy Imbert, Eric Deshaies, Veronique Barban, Alain Sabouraud, Jean-Philippe Barbe, Laurent Mallet
JOURNAL OF VIROLOGICAL METHODS
(2018)
Article
Virology
Veronique Barban, Nathalie Mantel, Aymeric De Montfort, Anke Pagnon, Fabrine Pradezynski, Jean Lang, Florence Boudet
JOURNAL OF VIROLOGY
(2018)
Article
Public, Environmental & Occupational Health
Bruno Guy, Veronique Barban, Nathalie Mantel, Marion Aguirre, Sandrine Gulia, Jeremy Pontvianne, Therese-Marie Jourdier, Laurence Ramirez, Veronique Gregoire, Christophe Charnay, Nicolas Burdin, Rafaele Dumas, Jean Lang
AMERICAN JOURNAL OF TROPICAL MEDICINE AND HYGIENE
(2009)
Article
Biochemical Research Methods
N. Mantel, M. Aguirre, S. Gulia, Y. Girerd-Chambaz, S. Colombani, C. Moste, V. Barban
JOURNAL OF VIROLOGICAL METHODS
(2008)
Article
Immunology
N. Mantel, Y. Girerd, C. Geny, I. Bernard, J. Pontvianne, J. Lang, V. Barban
Article
Public, Environmental & Occupational Health
Maria Kazimirova, Nathalie Mantel, Sandrine Raynaud, Mirko Slovak, Katarina Ustanikova, Jean Lang, Bruno Guy, Veronique Barban, Milan Labuda
VECTOR-BORNE AND ZOONOTIC DISEASES
(2012)
Article
Virology
Veronique Barban, Jorge L. Munoz-Jordan, Gilberto A. Santiago, Nathalie Mantel, Yves Girerd, Sandrine Gulia, Jean-Baptiste Claude, Jean Lang
Article
Immunology
Fabienne Piras-Douce, Franck Raynal, Alix Raquin, Yves Girerd-Chambaz, Sylviane Gautheron, Martha Erika Navarro Sanchez, Manuel Vangelisti, Nathalie Mantel
Summary: vYF-247, a live-attenuated yellow fever vaccine candidate, demonstrated low neurovirulence, neurotropism, viscerotropism, and comparable immunogenicity to Stamaril and YF-VAX in relevant animal models. In animal studies, vYF-247 provided protection against yellow fever virus and showed promising results as a potential vaccine candidate.