The dipole blockade of multiple Rydberg excitations in mesoscopic atomic ensembles allows the implementation of various quantum information tasks using collective states of cold, trapped atoms. Precise coherent manipulations of the collective ground and single Rydberg excitation states of an atomic ensemble requires the knowledge of the number of atoms with small uncertainty. We present an efficient method to acquire such information by interrogating the atomic ensemble with resonant pulses while monitoring the resulting Rydberg excitations. We show that after several such steps accompanied by feedback the number of atoms in an ensemble can be assessed with high accuracy. This will facilitate the realization of high-fidelity quantum gates, long-term storage of quantum information, and deterministic sources of single photons with Rydberg-blockaded atomic ensembles.
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