期刊
PHYSICAL CHEMISTRY CHEMICAL PHYSICS
卷 15, 期 16, 页码 6122-6127出版社
ROYAL SOC CHEMISTRY
DOI: 10.1039/c3cp50758k
关键词
-
资金
- EPSRC [EP/H029591/1] Funding Source: UKRI
- Engineering and Physical Sciences Research Council [EP/H029591/1, EP/C52389X/1] Funding Source: researchfish
We have used the formation of a bio-probe stack with up to 24 steps on gold nanoparticle and continuous gold surfaces to characterize the penetration depth of the plasmon field in a non-invasive manner by only involving biomolecules from standard bio-assays. An alternating anti-goat rabbit IgG and anti-rabbit IgG bio-probe stack is polymerized on protein A/G functionalized gold surfaces. The change in plasmon excitation angle or light scattering decreases exponentially with each stacking step although the bio-integrity of the antibody epitope is maintained. The exponential decay in the derived kinetic parameters is attributed to the change in the penetration depth and the step size is calibrated using a commercial continuous gold surface plasmon resonance surface to be 17.5 +/- 0.8 nm, consistent with the expected dimension of the antibody. The penetration depth of the gold spherical nanoparticles of diameter 90 +/- 13 nm is determined to be 93 +/- 10 nm.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据