Article
Chemistry, Multidisciplinary
Bo Zhuang, Daisuke Seo, Alexey Aleksandrov, Marten H. Vos
Summary: This study investigates the key reactive intermediates in flavoproteins using ultrafast spectroscopic studies, revealing that ultrafast electron transfer from Tyr50 to the excited FAD cofactor in WT BsFNR occurs within approximately 260 fs. The effects of protein environment and relaxations on the spectral properties of radical intermediates are explored, demonstrating the sensitivity of the spectral features of radical anionic FAD to its environment and the nature of counterions formed in the photoproducts. Overall, comprehensive characterizations of important radical intermediates involved in functional processes of flavoproteins are provided.
JOURNAL OF THE AMERICAN CHEMICAL SOCIETY
(2021)
Article
Biochemistry & Molecular Biology
H. Doerpholz, S. Subramanian, A. Zouni, F. Lisdat
Summary: In this study, photobioelectrodes based on a ferredoxin-modified photosystem I (PSI-Fd) from Thermosynechococcus vestitus were prepared and characterized. The direct electron transfer between PSI-Fd and the electrode was investigated. The results showed that the modified PSI-Fd photobioelectrode had a lower photocurrent and a lower onset potential of the cathodic photocurrent compared to native PSI, mainly due to the different adsorption behavior of the PSI-Fd construct onto the 3D ITO electrode. However, the overall behavior was similar to PSI. First experiments demonstrated the usage of the PSI-Fd photobioelectrode for enzyme-driven NADPH generation.
BIOELECTROCHEMISTRY
(2023)
Article
Biology
Manuela Kramer, Melvin Rodriguez-Heredia, Francesco Saccon, Laura Mosebach, Manuel Twachtmann, Anja Krieger-Liszkay, Chris Duffy, Robert J. Knell, Giovanni Finazzi, Guy Thomas Hanke
Summary: This study demonstrates the membrane association of FNR and its distribution depending on interaction with specific tether proteins. There is a correlation between FNR's interaction with different proteins and the activity of alternative photosynthetic electron transport pathways, supporting a role for FNR location in regulating photosynthetic electron flow during the transition from dark to light.
Article
Chemistry, Physical
Hitesh Medipally, Alice Guarneri, Lars Pospisil, Maurice C. R. Franssen, Willem J. H. Van Berkel, Caroline E. Paul, Marc M. Nowaczyk
Summary: We developed a light-driven approach based on photosystem I (PSI) to mimic the natural electron transfer process and regenerate NADPH. An illumination with red light led to successful reduction of NADP(+) to NADPH at pH 7.5. The light-driven NADPH regeneration system, coupled with three oxidoreductases, achieved high conversion rates and enantioselectivity in the asymmetric reduction reactions of C=C and C=O bonds.
Article
Biochemistry & Molecular Biology
Hitesh Medipally, Marvin Mann, Carsten Koetting, Willem J. H. van Berkel, Marc M. Nowaczyk
Summary: Photosynthetic organisms use light for the regeneration of NADPH. In this study, a clickable fusion system was established to regenerate NADPH with PSI-Fd and PSI-Fd-FNR. Immunity protein 7 was fused to the PSI-PsaE subunit, and colicin DNase E7 fusion chimeras of Fd and FNR were expressed. The complexes showed the ability to regenerate NADPH, demonstrating the applicability of this strategy.
Article
Biophysics
Stanislav D. Zakharov, Sergei Savikhin, Yuko Misumi, Genji Kurisu, William A. Cramer
Summary: This study confirms the absence of a significant interaction between FNR and the b(6)f complex through thermodynamic analysis. It also provides a precedent for considering protein-detergent interactions in ITC analyses.
BIOPHYSICAL JOURNAL
(2022)
Article
Plant Sciences
Melvin Rodriguez-Heredia, Francesco Saccon, Sam Wilson, Giovanni Finazzi, Alexander Ruban, Guy T. Hanke
Summary: Overexpression of photosynthetic enzyme Ferredoxin:NADP(H) reductase (FNR) increases plant tolerance to high light and oxidative stress, and its localization plays a role in repairing damages to photosystem I (PSI) during stress.
Article
Plant Sciences
Xinyi Wu, Jianqiang Wu, Yu Wang, Meiwen He, Mingming He, Weikang Liu, Sheng Shu, Jin Sun, Shirong Guo
Summary: In this study, it was found that PGR5 in cucumber plays a dominant role in cyclic electron flow, interacting with other proteins and being partially related to state transitions. PGR5 is a small, mobile protein that functions when attached to protein complexes.
HORTICULTURE RESEARCH
(2021)
Article
Plant Sciences
Magda Grabsztunowicz, Marjaana Rantala, Aiste Ivanauskaite, Tiina Blomster, Minna M. Koskela, Katariina Vuorinen, Esa Tyystjarvi, Meike Burow, Kirk Overmyer, Ari P. Mahonen, Paula Mulo
Summary: The study reveals the distinct expression patterns and accumulation locations of RFNR1 and RFNR2 in response to different stresses in Arabidopsis, indicating their essential roles in plant development and survival. Although the functions of RFNR1 and RFNR2 are partially redundant, both proteins are crucial for plant growth and adaptation to environmental challenges.
PLANT CELL AND ENVIRONMENT
(2021)
Article
Biotechnology & Applied Microbiology
Menglin Guo, Zhizhuo Shao, Zhenbing Zheng, Guangce Wang
Summary: In this study, the relationship between Ferredoxin-NADP+ oxidoreductase (FNR) and the oxidized pentose phosphate pathway (OPPP) in Neopyropia yezoensis was investigated. Transgenic strains of N. yezoensis with overexpression and silence of FNR were obtained. The results showed that reduction of FNR content can enhance OPPP, which further promotes the cyclic electron transportation (CET) operation. This study demonstrates the close relationship between photosynthetic electron transportation and carbohydrate metabolism in a strong resurrection plant.
ALGAL RESEARCH-BIOMASS BIOFUELS AND BIOPRODUCTS
(2023)
Article
Biochemistry & Molecular Biology
Mindaugas Lesanavicius, Daisuke Seo, Narimantas Cenas
Summary: RpFNR is a novel thioredoxin reductase-type FNR with partly characterized redox properties. It can be oxidized by various nonphysiological electron acceptors, and the reactivity is influenced by the single-electron reduction midpoint potential of the compounds.
Review
Biochemistry & Molecular Biology
Takashi Iyanagi
Summary: This article compares the roles of FNR-Fd and FNR-Fld complex pairs in plants with the diflavin reductase (FAD-FMN) family and discusses the evolutionary aspects of NAD(P)H-dependent multi-domain electron transfer systems.
Review
Plant Sciences
Yi-Fang Chiu, Hsiu-An Chu
Summary: This article provides an overview of the latest research progress on the structural and functional roles of Cytb(559) in photosystem II, highlighting the importance of cytochrome b(559) in the early assembly of photosystem II and secondary electron transfer pathways.
FRONTIERS IN PLANT SCIENCE
(2022)
Article
Plant Sciences
Tapio Lempiainen, Eevi Rintamaki, Eva-Mari Aro, Mikko Tikkanen
Summary: Photosynthetic light reactions require strict regulation under dynamic environmental conditions. Depending on environmental constraints, photoinhibition of either Photosystem II (PSII) or Photosystem I (PSI) occurs frequently. Repair of photodamaged PSI is very slow compared to that of PSII, leading to a functional imbalance between the two photosystems. In this study, we investigated the acclimation of photosynthesis to PSI-limited conditions, both in the short-term and long-term, after PSI-specific photoinhibition treatment. We found that short-term acclimation mechanisms were induced immediately after inhibition, while longer-term acclimation involved changes in the stromal redox system and increased abundance of ATP synthase and Cytochrome b6f. Acclimation to PSI-limited conditions restored the CO2 assimilation capacity of plants without major PSI repair. These findings demonstrate that plants efficiently acclimate to changes in the photosynthetic apparatus, which is crucial for their adaptation to adverse environmental conditions.
PLANT CELL AND ENVIRONMENT
(2022)
Article
Chemistry, Multidisciplinary
Christine M. Lewis, Justin D. Flory, Thomas A. Moore, Ana L. Moore, Bruce E. Rittmann, Wim F. J. Vermaas, Cesar Torres, Petra Fromme
Summary: A novel microbial electro-photosynthetic system (MEPS) is presented, which uses redox mediators and electrodes to drive electron transport in live cells. The results show that light-dependent current can be generated at high light intensities, and the electron delivery rate exceeds that of wildtype photosystem II (PSII). Furthermore, electron delivery occurs before the cytochrome b(6)f complex, enabling the production of both NADPH and ATP.
JOURNAL OF THE AMERICAN CHEMICAL SOCIETY
(2022)
Article
Plant Sciences
Helena Janeckova, Alexandra Husickova, Dusan Lazar, Ursula Ferretti, Pavel Pospisil, Martina Spundova
PLANT PHYSIOLOGY AND BIOCHEMISTRY
(2019)
Review
Plant Sciences
Alexandrina Stirbet, Dusan Lazar, Ya Guo, Govindjee Govindjee
Article
Plant Sciences
M. Bednarikova, Y. Folgar-Camean, Z. Kucerova, D. Lazar, M. Spundova, J. Hajek, M. Bartak
Article
Biotechnology & Applied Microbiology
Barbora Barankova, Dusan Lazar, Jan Naus, Alexei Solovchenko, Olga Gorelova, Olga Baulina, Gregor Huber, Ladislav Nedbal
ALGAL RESEARCH-BIOMASS BIOFUELS AND BIOPRODUCTS
(2020)
Article
Plant Sciences
Michaela Bednarikova, Peter Vaczi, Dusan Lazar, Milos Bartak
PHOTOSYNTHESIS RESEARCH
(2020)
Article
Plant Sciences
David Fuente, Dusan Lazar, Jose Vicente Oliver-Villanueva, Javier F. Urchueguia
Summary: The study reconstructed the absorption spectrum of different strains by summing the computed signature of all pigments present in the organism. Modifications to in vitro pigment spectra were made and a plausible shape for the in vivo absorption spectrum of each chromophore was outlined. The study also provided estimates of pigment concentrations without relying on spectrophotometric correlations, and accurately reproduced experimental spectra for studied wavelengths in various strains.
PHOTOSYNTHESIS RESEARCH
(2021)
Review
Plant Sciences
D. Lazar, A. Stirbet, L. O. Bjorn, G. Govindjee
Summary: Oxygenic photosynthesis occurs in the thylakoid membranes, where light is absorbed by pigments and transferred to the photosynthetic reaction centers. The color and intensity of light exposure affect the composition and structure of light-harvesting complexes, thus influencing the photosynthetic process and the organism as a whole.
Correction
Plant Sciences
Iva Ilikova, Petr Ilik, Monika Opatikova, Rameez Arshad, Lukas Nosek, Valav Karlicky, Zuzana Kucerova, Pavel Roudnicky, Pavel Pospisil, Dusan Lazar, Jan Bartos, Roman Kouril
Article
Plant Sciences
Hui Lyu, Dusan Lazar
Summary: The transthylakoid membrane potential plays a crucial role in driving ATP synthesis and regulating photosynthesis under environmental stresses, with cation binding influencing the electric potentials adjustment. However, a comprehensive understanding of the impact of altered cationic binding on the membrane surface is still lacking.
FRONTIERS IN PLANT SCIENCE
(2022)
Article
Plant Sciences
Dusan Lazar, Yuxi Niu, Ladislav Nedbal
Summary: This study exposed pea leaves to oscillating light and found that the dynamics of photosynthesis depend on the oscillation period, revealing information about the underlying regulatory networks. The regulation aims to keep the reaction centers of PSI and PSII open. Further experiments, mathematical modeling, and system identification and control tools are expected to enhance our understanding of these regulatory networks.
JOURNAL OF EXPERIMENTAL BOTANY
(2022)
Article
Plant Sciences
Hui Lyu, Dusan Lazar
Summary: The light-induced transthylakoid membrane potential (Delta psi) plays a crucial role in ATP synthesis and in the acclimation of photosynthesis to changing light conditions. However, the mechanisms underlying ion fluxes and their impact on Delta psi regulation remain unclear.
PLANT PHYSIOLOGY AND BIOCHEMISTRY
(2023)
Article
Plant Sciences
Yuxi Niu, Dusan Lazar, Alfred R. Holzwarth, David M. Kramer, Shizue Matsubara, Fabio Fiorani, Hendrik Poorter, Silvia D. Schrey, Ladislav Nedbal
Summary: The study focused on the rapid regulation of photoprotective mechanisms in response to changing light in plants. It was found that PsbS regulation had the fastest response time to oscillating light with periods longer than 10 seconds. Processes involving violaxanthin de-epoxidase dampened changes in chlorophyll fluorescence in oscillation periods of 2 minutes or longer. Knocking out the PGR5/PGRL1 pathway strongly reduced variations in all monitored parameters, possibly due to congestion in the electron transport. The observed dynamics of photosystem I components were interpreted as being formed by thylakoid remodeling in slow light oscillations.
Article
Plant Sciences
Iva Ilikova, Petr Ilik, Monika Opatikova, Rameez Arshad, Lukas Nosek, Vaclav Karlicky, Zuzana Kucerova, Pavel Roudnicky, Pavel Pospisil, Dusan Lazar, Jan Bartos, Roman Kouril
Summary: The study examined the structure-function relationship of PSII supercomplexes in Arabidopsis mutants to explore the possibility of spruce-type PSII supercomplex formation in angiosperms. Unlike in spruce, both LHCB3 and LHCB6 were found to be necessary for stable binding of the M trimer to the PSII core in Arabidopsis. The formation of spruce-type PSII supercomplex was observed with low abundance only in Ihcb3 plants, suggesting a unique structural arrangement in this mutant.
Article
Plant Sciences
Ladislav Nedbal, Dusan Lazar
Summary: Frequency-domain measurements of plant responses to sinusoidally modulated light can provide valuable information without the need for dark acclimation, targeting different kinetic domains and regulatory feedbacks through analysis of amplitudes, phase shifts, and upper harmonic modulation for a wide range of frequencies. This approach can be a versatile tool in plant sensing, reflecting nonlinear phenomena such as photosynthetic regulation.
Review
Plant Sciences
Alexandrina Stirbet, Lars Olof Bjorn, Dmitry Shevela, Suleyman Allakhverdiev, Arthur Nonomura, Xin-Guang Zhu, Dusan Lazar, Ashwani Pareek, Gyozo Garab, Julian J. Eaton-Rye
NEW ZEALAND JOURNAL OF BOTANY
(2020)