Article
Engineering, Chemical
Christopher J. Yehl, Andrew L. Zydney
Summary: This study explores a novel approach for protein separations based on High Performance Countercurrent Membrane Purification (HPCMP), demonstrating the feasibility of achieving high selectivity and over 98% yield of proteins without significant pressure-driven flow. The results suggest the potential of utilizing HPCMP for high resolution separations in the preparation of biopharmaceuticals and natural protein products.
JOURNAL OF MEMBRANE SCIENCE
(2021)
Article
Cell Biology
Sergey K. Zharmukhamedov, Mehriban S. Shabanova, Margarita Rodionova, Irada M. Huseynova, Mehmet Sayim Karacan, Nurcan Karacan, Kubra Begum Asik, Vladimir D. Kreslavski, Saleh Alwasel, Suleyman Allakhverdiev
Summary: The effects of the [CuL2]Br-2 complex on PSII activity and chlorophyll fluorescence yield in spinach were investigated. The complex was found to inhibit photosynthetic oxygen evolution and the photoreduction of the primary quinone electron acceptor. The inhibition depended on the concentration of [CuL2]Br-2 and was not affected by artificial electron donors.
Article
Biotechnology & Applied Microbiology
Laura Soto-Sierra, Zivko L. Nikolov
Summary: This study evaluates tangential flow ultrafiltration as an alternative method for producing protein hydrolysates. The results show that the membrane diafiltration process has the potential to efficiently separate and recover hydrolyzed protein, with a higher protein recovery yield compared to centrifugation and depth filtration methods. Additionally, protein hydrolysates obtained by membrane ultrafiltration exhibit slightly better heat and pH stability.
FRONTIERS IN BIOENGINEERING AND BIOTECHNOLOGY
(2022)
Article
Plant Sciences
Akihiro Saito, Kimika Hoshi, Yuna Wakabayashi, Takumi Togashi, Tomoki Shigematsu, Maya Katori, Takuji Ohyama, Kyoko Higuchi
Summary: The barley cultivar Sarab 1 can continue photosynthesis under low iron conditions through a reduction in P700 over-reduction and an increase in non-appressed thylakoid membranes. In contrast, another cultivar, Ehimehadaka-1, preferentially supplies iron to photosystem I to maintain photosynthetic activity. These findings highlight the multiple strategies that barley species have for acclimating photosynthesis to iron deficiency.
Article
Materials Science, Multidisciplinary
Joshua M. Passantino, Alessia M. Williams, Marc A. Nabhan, David E. Cliffel, G. Kane Jennings
Summary: This study demonstrates the successful utilization of PSI protein in photoactive polymerization, creating a photoactive and conductive protein-polymer composite. The stability and successful reaction of this composite material are confirmed through various testing methods.
ACS APPLIED POLYMER MATERIALS
(2022)
Article
Biochemistry & Molecular Biology
Taro Shimamoto, Tatsuki Nakakubo, Tomoyasu Noji, Shuhei Koeda, Keisuke Kawakami, Nobuo Kamiya, Toshihisa Mizuno
Summary: Research on a new solubilization surfactant DKDKC12K-PA(n) that can maintain binding with membrane proteins in aqueous buffer has potential applications in preserving the structure and function of solubilized membrane proteins.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Engineering, Environmental
Carmen M. Sanchez-Arevalo, Maria Cinta Vincent-Vela, Maria-Jose Lujan-Facundo, Silvia Alvarez-Blanco
Summary: Among all membrane processes, ultrafiltration is widely used for both aqueous filtrations and solvent-based solutions. However, the challenges of membrane contact with organic solvents, such as permeate flux, rejection values, and long-term stability, remain. This review examines the effects of solvents on the ultrafiltration process and focuses on membrane pre-treatment strategies. It provides valuable knowledge and insights into organic solvent ultrafiltration.
PROCESS SAFETY AND ENVIRONMENTAL PROTECTION
(2023)
Article
Plant Sciences
Tapio Lempiainen, Eevi Rintamaki, Eva-Mari Aro, Mikko Tikkanen
Summary: Photosynthetic light reactions require strict regulation under dynamic environmental conditions. Depending on environmental constraints, photoinhibition of either Photosystem II (PSII) or Photosystem I (PSI) occurs frequently. Repair of photodamaged PSI is very slow compared to that of PSII, leading to a functional imbalance between the two photosystems. In this study, we investigated the acclimation of photosynthesis to PSI-limited conditions, both in the short-term and long-term, after PSI-specific photoinhibition treatment. We found that short-term acclimation mechanisms were induced immediately after inhibition, while longer-term acclimation involved changes in the stromal redox system and increased abundance of ATP synthase and Cytochrome b6f. Acclimation to PSI-limited conditions restored the CO2 assimilation capacity of plants without major PSI repair. These findings demonstrate that plants efficiently acclimate to changes in the photosynthetic apparatus, which is crucial for their adaptation to adverse environmental conditions.
PLANT CELL AND ENVIRONMENT
(2022)
Article
Chemistry, Applied
Ozgenur Coskun, Lars Wiking, Saeed Rahimi Yazdi, Milena Corredig
Summary: The study obtained MPI and MCC using UF and MF, respectively, and assessed the effect of diafiltration on the colloidal dissociation of casein micelles. Comparison of serum phases with and without whey protein revealed significant differences in ionic strength and soluble proteins in the final concentrates, indicating potential indicators of ingredient quality and functionality.
FOOD HYDROCOLLOIDS
(2022)
Article
Multidisciplinary Sciences
Yingke Liang, Alicia Plourde, Stephanie A. Bueler, Jun Liu, Peter Brzezinski, Siavash Vahidi, John L. Rubinstein
Summary: Oxidative phosphorylation is a promising target for treating Mycobacterium tuberculosis and other mycobacteria infections. The structure of the mycobacterial electron transport chain reveals the presence of several important components, including the orphan protein MSMEG_2064, acyl phosphati-dylinositol dimannoside, menaquinone, and a purine nucleoside triphosphate. These findings provide insights into the function and structure of the enzyme.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2023)
Article
Plant Sciences
Helena Sapeta, Makio Yokono, Atsushi Takabayashi, Yoshifumi Ueno, Andre M. Cordeiro, Toshihiko Hara, Ayumi Tanaka, Seiji Akimoto, M. Margarida Oliveira, Ryouichi Tanaka
Summary: Jatropha curcas responds to drought by increasing thermal dissipation of excitation energy in both photosystems, temporarily down-regulating the quantum yields of both PSII and PSI. This helps protect the photosynthetic machinery and enables rapid recovery of photosynthetic capacity when water is available.
JOURNAL OF EXPERIMENTAL BOTANY
(2023)
Article
Biotechnology & Applied Microbiology
Zulhaj Rizki, Richard Ravesloot, Ruud van Beckhoven, Marcel Ottens
Summary: The UF/DF process is crucial for isolating canola protein from rapeseed meal. By using a multi-stage membrane system, the components of the mixture can be separated. Diafiltration, which dilutes the feed stream, requires a substantial amount of water. This study used a mechanistic model and a genetic algorithm to optimize the process variables and improve the performance of a four stage UF/DF system, resulting in a reduction in diafiltrate, an increase in purity, and an increase in dry matter content.
FOOD AND BIOPRODUCTS PROCESSING
(2023)
Article
Biochemistry & Molecular Biology
Maria Fernanda Montenegro-Landivar, Paulina Tapia-Quiros, Xanel Vecino, Monica Reig, Merce Granados, Adriana Farran, Jose Luis Cortina, Javier Saurina, Cesar Valderrama
Summary: This study focuses on the recovery of polyphenols from spinach and orange waste using membrane technology. A treatment sequence using selective membranes was proposed to obtain polyphenol-rich streams for various applications and recover clean water streams.
Article
Chemistry, Applied
Ming Cai, Haoyong Xing, Baoming Tian, Jing Xu, Zhenhao Li, Hua Zhu, Kai Yang, Peilong Sun
Summary: The study revealed that Ganoderma lucidum polysaccharides can extend swimming time, enhance endurance, and promote fatigue recovery, especially polysaccharides with molecular weights above 10 kDa. These GLPs may reduce the activities of SUN and CK, decrease the levels of MDA and BLA, increase the level of Gly, accelerate fat transformation, and improve the activities of GPx, SOD, and LDH in all treated mice. Therefore, GLPs above 10 kDa could be potential agents with antifatigue activity.
CARBOHYDRATE POLYMERS
(2021)
Article
Engineering, Biomedical
Kasper Kristensen, Rasmus Munter, Paul J. Kempen, Mikkel E. Thomsen, Allan Stensballe, Thomas L. Andresen
Summary: By investigating the efficiency of three commonly used liposome isolation methods, it was revealed that previous research on the liposomal protein corona may have been impacted by significant methodological problems, particularly related to contaminant proteins interfering with the analysis of the protein corona.
ACTA BIOMATERIALIA
(2021)
Article
Engineering, Biomedical
Bo Zhang, Naresh Kasoju, Qiongfang Li, Erfan Soliman, Aidong Yang, Zhanfeng Cui, Jinmin Ma, Hui Wang, Hua Ye
BIOMEDICAL MATERIALS
(2019)
Review
Biotechnology & Applied Microbiology
Julian George, Chia-Chen Hsu, Linh Thuy Ba Nguyen, Hua Ye, Zhanfeng Cui
BIOTECHNOLOGY ADVANCES
(2020)
Article
Engineering, Environmental
Jibin Yang, Huicai Wang, Zhongan Tao, Xiaping Liu, Zhenwen Wang, Ruirui Yue, Zhanfeng Cui
CHEMICAL ENGINEERING JOURNAL
(2019)
Article
Biochemical Research Methods
Catherine Fan, Yin-Hu Wu, Christoph M. Decker, Reza Rohani, Manuela Gesell Salazar, Hua Ye, Zhanfeng Cui, Frank Schmidt, Wei E. Huang
ACS SYNTHETIC BIOLOGY
(2019)
Article
Limnology
Ma Chengbo, Li Wenjun, Ge Baosheng, Lin Jian, Qin Song
JOURNAL OF OCEANOLOGY AND LIMNOLOGY
(2020)
Article
Biotechnology & Applied Microbiology
Zhijin Wei, Haonan Wang, Xiao Li, Qianqian Zhao, Yonghao Yin, Lijun Xi, Baosheng Ge, Song Qin
JOURNAL OF APPLIED PHYCOLOGY
(2020)
Article
Biochemistry & Molecular Biology
Qian Yu, Haonan Wang, Xiao Li, Yonghao Yin, Song Qin, Baosheng Ge
PROCESS BIOCHEMISTRY
(2020)
Article
Nanoscience & Nanotechnology
Hua He, Xinjian Shi, Junying Wang, Xiaojuan Wang, Qian Wang, Daoyong Yu, Baosheng Ge, Xiaodong Zhang, Fang Huang
ACS APPLIED MATERIALS & INTERFACES
(2020)
Article
Multidisciplinary Sciences
Catherine Fan, Paul A. Davison, Robert Habgood, Hong Zeng, Christoph M. Decker, Manuela Gesell Salazar, Khemmathin Lueangwattanapong, Helen E. Townley, Aidong Yang, Ian P. Thompson, Hua Ye, Zhanfeng Cui, Frank Schmidt, C. Neil Hunter, Wei E. Huang
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2020)
Article
Biotechnology & Applied Microbiology
Akinlolu Oyekunle Oluseun Odeleye, Timothee Baudequin, Chih-Yao Chui, Zhanfeng Cui, Hua Ye
BIOCHEMICAL ENGINEERING JOURNAL
(2020)
Article
Chemistry, Medicinal
Baosheng Ge, Haonan Wang, Jie Li, Hengheng Liu, Yonghao Yin, Naili Zhang, Song Qin
Article
Biotechnology & Applied Microbiology
Wei E. Huang, Boon Lim, Chia-Chen Hsu, Dan Xiong, Wei Wu, Yejiong Yu, Huidong Jia, Yun Wang, Yida Zeng, Mengmeng Ji, Hong Chang, Xiuming Zhang, Hui Wang, Zhanfeng Cui
MICROBIAL BIOTECHNOLOGY
(2020)
Article
Engineering, Chemical
Yejiong Yu, Aidong Yang, Hua Ye, Julian F. Dye, Zhanfeng Cui
CHEMICAL ENGINEERING SCIENCE
(2020)
Article
Engineering, Biomedical
Zhanfeng Cui, Hong Chang, Hui Wang, Boon Lim, Chia-Chen Hsu, Yejiong Yu, Huidong Jia, Yun Wang, Yida Zeng, Mengmeng Ji, Weizhi Liu, Catriona Inverarity, Wei E. Huang
BIO-DESIGN AND MANUFACTURING
(2020)
Article
Biochemistry & Molecular Biology
Ma Haotian, Shuaihang Chen, Huaye Xiong, Meng Wang, Wei Hang, Xiaoli Zhu, Yubin Zheng, Baosheng Ge, Runzhi Li, Hongli Cui