Article
Nanoscience & Nanotechnology
Janet E. Sorrells, Rishyashring R. Iyer, Lingxiao Yang, Elisabeth M. Martin, Geng Wang, Haohua Tu, Marina Marjanovic, Stephen A. Boppart
Summary: This paper presents a computational photon counting method using a hybrid photodetector and multithreshold peak detection, which enables faster imaging. By counting instances where photons arrive at the detector within the detector response time, the method accurately characterizes the intensity and fluorescence lifetime of samples.
Article
Biochemistry & Molecular Biology
Thomas Kellerer, Janko Janusch, Christian Freymueller, Adrian Ruehm, Ronald Sroka, Thomas Hellerer
Summary: This study utilized two different fluorescence lifetime measurement techniques to investigate the factors affecting fluorescence lifetime results, showing consistent results and revealing the strongest influencing factor on fluorescence lifetime. It also provides quantitative guidance on how to choose the most suitable technique and perform experiments correctly to obtain consistent fluorescence lifetime results.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Nanoscience & Nanotechnology
Wenxuan Liang, Defu Chen, Honghua Guan, Hyeon-Cheol Park, Kaiyan Li, Ang Li, Ming-Jun Li, Israel Gannot, Xingde Li
Summary: This article introduces a newly developed two-photon fluorescence lifetime imaging endomicroscope that can extract NADH lifetime parameters of living tissues and organs, track changes in cellular metabolic status and tumor models, and promises real-time dynamic imaging in clinical diagnosis and therapeutic applications.
Article
Optics
Sandro Heuke, Carla Silva Martins, Remi Andre, Loic Legoff, Herve Rigneault
Summary: Two-photon excited fluorescence (2PEF) microscopy is a popular nonlinear imaging method for biomedical samples. It uses multiple detectors and spectral filter sets to discriminate different fluorophores based on their emission behavior. However, 2PEF suffers from the loss of fluorescence photons outside the filter transmission range and may fail to discriminate fluorophores with similar emission profiles. Here, we propose an alternative 2PEF method that discriminates fluorophores based on their excitation spectra.
Article
Chemistry, Analytical
Wei Yan, Yangrui Huang, Luwei Wang, Jin Li, Yong Guo, Zhigang Yang, Junle Qu
Summary: This study demonstrates a multi-color two-photon imaging method with single-wavelength excitation. The problem of spectral crosstalk is effectively solved by selecting suitable filter combinations and applying image subtraction. The results show that two-color and three-color two-photon imaging can be achieved using a single femtosecond laser.
Article
Optics
Jiri Junek, Karel Zidek
Summary: Using a random temporal signal for sample excitation (RATS method) is a new approach to measure photoluminescence (PL) dynamics. This article analyzes the noise effect on the RATS method and suggests strategies for noise suppression. The results show that the PL signal noise level is critical and increasing the acquisition time is beneficial, while increasing the number of measurements only has a minimal effect.
Article
Biochemistry & Molecular Biology
Andrew H. A. Clayton
Summary: The dynamics of condensed matter can be studied using the time-dependent Stokes shift of a fluorescent probe. The time-dependent spectral correlation function is described by spectral relaxation correlation times that characterize the relaxation processes around the excited-state probe. Phasor plot is a powerful tool to analyze time and frequency-domain data, providing spatial maps of spectral dynamics in complex structures like living cells. The analysis workflow also addresses the extraction of initial and relaxed generalized polarization and improved methods for model discrimination and parameter extraction in complex spectral relaxation phenomena.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Engineering, Electrical & Electronic
Nehad Hirmiz, Anthony Tsikouras, Elizabeth J. Osterlund, Morgan Richards, David W. Andrews, Qiyin Fang
Summary: A high-speed multiplexed confocal microscope system has been developed for measuring protein-protein interactions and generating binding curves in live cells. The results demonstrate the system's effectiveness in screening for protein-protein interactions in drug discovery applications.
IEEE JOURNAL OF SELECTED TOPICS IN QUANTUM ELECTRONICS
(2021)
Review
Chemistry, Physical
Janah Shaya, Peter R. Corridon, Basem Al-Omari, Abdulrahman Aoudi, Abeer Shunnar, Mohamed Infas Haja Mohideen, Ahsanulhaq Qurashi, Benoit Y. Michel, Alain Burger
Summary: Two-photon fluorescence microscopy (2PFM) is a powerful alternative to traditional one-photon microscopy, using two near-infrared photons to excite fluorescent dyes and improve imaging quality in biological samples. Fluorene dyes possess excellent properties for 2PFM probes, including enhanced solubility, photostability, and two-photon absorption cross-section.
JOURNAL OF PHOTOCHEMISTRY AND PHOTOBIOLOGY C-PHOTOCHEMISTRY REVIEWS
(2022)
Article
Engineering, Environmental
Adrian Monteleone, Folker Wenzel, Heinz Langhals, Daniel Dietrich
Summary: The FLIM method was tested for identification and characterization of six types of plastics, showing significant differentiation in 94.55% of the comparisons. Results suggest that FLIM has the potential for sub-micrometer plastic characterization and allows for visual 3D-sectioning of samples, which could be important for identifying and characterizing plastics in tissue and environmental samples.
JOURNAL OF ENVIRONMENTAL CHEMICAL ENGINEERING
(2021)
Article
Chemistry, Multidisciplinary
Aurimas Vysniauskas, Bethan Cornell, Peter S. Sherin, Karolina Maleckaite, Marketa Kubankova, Maria Angeles Izquierdo, Thanh Truc Vu, Yulia A. Volkova, Ekaterina M. Budynina, Carla Molteni, Marina K. Kuimova
Summary: A new cell-permeable fluorescence probe based on BODIPY was developed to respond to environmental temperature changes, with reduced viscosity dependence through functionalization with cyclopropyl substituents. This probe shows potential for applications in fluorescence lifetime imaging microscopy (FLIM) due to its temperature-dependent fluorescence decays.
Article
Obstetrics & Gynecology
Jaimin S. Shah, Marta Venturas, Tim H. Sanchez, Alan S. Penzias, Daniel J. Needleman, Denny Sakkas
Summary: The study found significant metabolic differences between euploid and aneuploid human blastocysts using FLIM, indicating potential for FLIM as a non-invasive clinical tool to assist in identifying ploidy status.
HUMAN REPRODUCTION
(2022)
Article
Chemistry, Multidisciplinary
Wei-Wen Chen, Wenyu Tang, Emily K. K. Hamerton, Penelope X. X. Kuo, George A. A. Lemieux, Kaveh Ashrafi, Marcus T. T. Cicerone
Summary: Fat metabolism is an important factor in the aging and longevity of Caenorhabditis elegans. A new approach using Nile Red and 2p-FLIM can effectively distinguish lipid particle sub-types in vivo. This method has the advantage of being able to use common, commercially available 2p-FLIM systems and requires only about 10% of the time needed for BCARS imaging. This work provides a novel and widely accessible method for analyzing lipid-containing structures in a complex live organism context.
FRONTIERS IN CHEMISTRY
(2023)
Article
Microbiology
Thierry Tran, Cosette Grandvalet, Pascale Winckler, Francois Verdier, Antoine Martin, Herve Alexandre, Raphaelle Tourdot-Marechal
Summary: Kombucha pellicles, produced by acetic acid bacteria and yeasts, show a layered structure where yeast cells act as scaffolding for bacterial cellulose accumulation. The sublayer rich in biomass is crucial for cellulose production and optimizing yeast-AAB metabolic interactions. Yeasts significantly impact the structure and properties of kombucha pellicles compared to those grown from pure AAB cultures.
FRONTIERS IN MICROBIOLOGY
(2021)
Article
Plant Sciences
Tim Rammler, Frank Wackenhut, Johanna Rapp, Sven Zur Oven-Krockhaus, Karl Forchhammer, Alfred J. J. Meixner, Klaus Harter
Summary: Photosynthesis is a vital biological process that produces oxygen and is the foundation for various plant products. Measurement of photosynthetic properties can provide insights into the physiological conditions of organisms. A new method using an optical Fabry-Perot microcavity allows for the assessment of photosynthetic activity and properties for individual cyanobacteria.
Article
Biophysics
Caner Unlu, Iryna Polukhina, Herbert van Amerongen
EUROPEAN BIOPHYSICS JOURNAL WITH BIOPHYSICS LETTERS
(2016)
Article
Chemistry, Physical
Shazia Farooq, Jevgenij Chmeliov, Gediminas Trinkunas, Leonas Valkunas, Herbert van Amerongen
JOURNAL OF PHYSICAL CHEMISTRY LETTERS
(2016)
Article
Plant Sciences
Reza Ranjbar Choubeh, Ravi R. Sonani, Datta Madamwar, Paul C. Struik, Arjen N. Bader, Bruno Robert, Herbert van Amerongen
PHOTOSYNTHESIS RESEARCH
(2018)
Article
Plant Sciences
Ivo H. M. van Stokkum, Michal Gwizdala, Lijin Tian, Joris J. Snellenburg, Rienk van Grondelle, Herbert van Amerongen, Rudi Berera
PHOTOSYNTHESIS RESEARCH
(2018)
Article
Plant Sciences
Lucilla Taddei, Volha U. Chukhutsina, Bernard Lepetit, Giulio Rocco Stella, Roberto Bassi, Herbert van Amerongen, Jean-Pierre Bouly, Marianne Jaubert, Giovanni Finazzi, Angela Falciatore
Article
Multidisciplinary Sciences
Frank J. Vergeldt, Alena Prusova, Farzad Fereidouni, Herbert van Amerongen, Henk van As, Tom W. J. Scheenen, Arjen N. Bader
SCIENTIFIC REPORTS
(2017)
Article
Plant Sciences
Tomas E. van den Berg, Volha U. Chukhutsina, Herbert van Amerongen, Roberta Croce, Bart van Oort
Article
Biochemistry & Molecular Biology
Reza Ranjbar Choubeh, Rob B. M. Koehorst, David Bina, Paul C. Struik, Jakub Psencik, Herbert van Amerongen
BIOCHIMICA ET BIOPHYSICA ACTA-BIOENERGETICS
(2019)
Article
Multidisciplinary Sciences
Vincenzo Mascoli, Ahmad Farhan Bhatti, Luca Bersanini, Herbert van Amerongen, Roberta Croce
Summary: Some cyanobacteria acclimate to far-red light by integrating chlorophyll f and producing red-shifted allophycocyanin. The presence of red-shifted allophycocyanin antennas speeds up charge separation in chlorophyll-f-containing Photosystem II, which is different from other photosynthetic systems.
NATURE COMMUNICATIONS
(2022)
Article
Plant Sciences
Dana Verhoeven, Herbert van Amerongen, Emilie Wientjes
Summary: Oxygenic photosynthesis is driven by light absorption in photosystems I and II, and state transitions serve to maintain the balance between them. This study used confocal fluorescence lifetime imaging to observe state transitions in single Arabidopsis chloroplasts. The results showed that the absorption ratio of photosystem I relative to photosystem II is higher in state 2. Additionally, the study found that the macro-organization of thylakoids is largely unaffected by state transitions.
Article
Plant Sciences
Christo Schiphorst, Luuk Achterberg, Rodrigo Gomez, Rob Koehorst, Roberto Bassi, Herbert van Amerongen, Luca Dall'Osto, Emilie Wientjes
Summary: The light-harvesting antennae of photosystem I facilitate energy transfer from trimeric light-harvesting complex II to photosystem I, and the presence of LHCI significantly enhances the efficiency of energy transfer.
Review
Multidisciplinary Sciences
Roberta Croce, Herbert van Amerongen
Article
Biochemistry & Molecular Biology
Inge Bos, Kaitlyn M. Bland, Lijin Tian, Roberta Croce, Laurie K. Frankel, Herbert van Amerongen, Terry M. Bricker, Emilie Wientjes
BIOCHIMICA ET BIOPHYSICA ACTA-BIOENERGETICS
(2017)
Article
Biochemistry & Molecular Biology
Emilie Wientjes, John Philippi, Jan Willem Borst, Herbert van Amerongen
BIOCHIMICA ET BIOPHYSICA ACTA-BIOENERGETICS
(2017)
Article
Plant Sciences
Shazia Farooq, Jevgenij Chmeliov, Emilie Wientjes, Rob Koehorst, Arjen Bader, Leonas Valkunas, Gediminas Trinkunas, Herbert van Amerongen