Article
Endocrinology & Metabolism
Fatih M. Uckun, Cemal Orhan, Mehmet Tuzcu, Ali Said Durmus, Ibrahim H. Ozercan, Michael Volk, Kazim Sahin
Summary: This study showed that GMP-grade RJX treatment can accelerate wound and burn healing in diabetic rats, indicating its potential clinical application.
FRONTIERS IN ENDOCRINOLOGY
(2022)
Article
Biochemistry & Molecular Biology
Kejia Wang, Kuo Shen, Fu Han, Xiaozhi Bai, Zhuoqun Fang, Yanhui Jia, Jian Zhang, Yan Li, Weixia Cai, Xujie Wang, Liang Luo, Kai Guo, Hongtao Wang, Xuekang Yang, Huina Wang, Dahai Hu
Summary: Deep second-degree burns heal slowly, and promoting the healing process is a focus of clinical research. This study explored the role and molecular mechanism of sestrin2 in deep second-degree burns as a potential treatment target for burn wounds.
ARCHIVES OF BIOCHEMISTRY AND BIOPHYSICS
(2023)
Article
Multidisciplinary Sciences
Michiharu Sakamoto, Takashi Nakano, Itaru Tsuge, Hiroki Yamanaka, Yasuhiro Katayama, Yoshihiro Shimizu, Yoshika Note, Masukazu Inoie, Naoki Morimoto
Summary: The study found that dried allogeneic cultured epidermis (CE) has similar morphological and physical properties as cryopreserved CE, and it can be a physiological and versatile wound dressing.
SCIENTIFIC REPORTS
(2022)
Article
Pharmacology & Pharmacy
Nabil A. Alhakamy, Giuseppe Caruso, Basma G. Eid, Usama A. Fahmy, Osama A. A. Ahmed, Ashraf B. Abdel-Naim, Abdulmohsin J. Alamoudi, Shareefa A. Alghamdi, Hadeel Al Sadoun, Basmah M. Eldakhakhny, Filippo Caraci, Wesam H. Abdulaal
Summary: The study investigates the potential of a nanoconjugate of CTX and MEL loaded in hydroxypropyl methylcellulose gel to improve wound healing in diabetic rats. The results show that the nanocomplex enhances epithelium regeneration, reduces oxidative stress, decreases pro-inflammatory cytokines, and increases collagen expression, highlighting its promising application in wound healing research.
Article
Multidisciplinary Sciences
Zhaoyan Chen, Ya Zeng, Fangyuan Tian
Summary: Animal experiments are crucial for advances in burn research and treatment. Currently, researchers often rely on personal experience or literature to construct animal disease models, resulting in a lack of scientific rigor and limited reference value. This study used the Delphi method to establish a rat model of third-degree burns, improving the overall quality of burn research.
SCIENTIFIC REPORTS
(2022)
Article
Plant Sciences
Xiao-yan Chen, Wen-wen Jiang, Yan-ling Liu, Zhao-xia Ma, Jian-qiang Dai
Summary: This study shows that geniposide promotes wound healing in diabetic rats through its anti-inflammatory action, reducing inflammatory cell infiltration and fibroblast proliferation. It also modulates the expression levels of pro-inflammatory factors. Further research is needed to evaluate its antibacterial activity and effects on skin regeneration.
PHARMACEUTICAL BIOLOGY
(2022)
Article
Dermatology
Ashok Priyadarshi, Gaurav K. K. Keshri, Asheesh Gupta
Summary: Combination therapy of PBMT and SBTL-ALE significantly promotes the healing of third-degree burn wounds in rats by enhancing wound area contraction, angiogenesis, dermal hydration, collagen synthesis, and redox homeostasis. It also reduces inflammation, oxidative stress, and LDH activity, while increasing energy metabolism. This multimode therapeutic strategy provides a novel approach for the treatment of burns and dermal cosmetic care.
JOURNAL OF COSMETIC DERMATOLOGY
(2023)
Article
Biochemistry & Molecular Biology
Juliana A. R. Moreira, Israel C. Vasconcelos, Jose L. Fachi, Viviane Theodoro, Rodrigo A. Dalia, Andrea A. Aro, Edson R. Pimentel, Fernanda O. G. Gaspi, Thiago A. M. Andrade, Maria E. C. Amaral, Marcelo A. M. Esquisatto, Fernanda A. S. Mendonca, Glaucia M. T. Santos
Summary: The combination therapy of Solidago chilensis and laser reduced inflammatory infiltrate and promoted angiogenesis in burns of diabetic rats. Laser or plant extract alone showed higher levels of VEGF. Low-level laser therapy increased collagen I and decreased collagen III, as well as activated MMP-2 and reduced active MMP-9 isoform.
BIOMEDICAL JOURNAL
(2021)
Article
Endocrinology & Metabolism
Maysa S. S. Campos, Jose B. Volpon, Joao Paulo B. Ximenez, Ana Paula Franttini, Christopher E. Dalloul, Manoel D. Sousa-Neto, Raquel A. A. Silva, Melissa A. A. Kacena, Ariane Zamarioli
Summary: This study investigated the effects of vibration therapy on fracture healing in diabetic and non-diabetic rats. The results showed that diabetes had detrimental effects on bone healing, but vibration therapy was effective at counteracting these effects in diabetic rats. Vibration therapy promoted osteogenic and chondrogenic cell proliferation, improved bone healing structure and quantity, and restored serum levels of certain factors involved in bone repair.
FRONTIERS IN ENDOCRINOLOGY
(2022)
Article
Cell Biology
Patricia Kasowanjete, Heidi Abrahamse, Nicolette N. Houreld
Summary: This study evaluated the effect of photobiomodulation (PBM) at 660 nm on wound healing in diabetic patients, and found that PBM can stimulate diabetic wound healing through activation of the Ras/MAPK pathway. This indicates that PBM has the potential to be an effective treatment for diabetic foot ulcers.
Article
Pharmacology & Pharmacy
Nabil A. Alhakamy, Giuseppe Caruso, Anna Privitera, Osama A. A. Ahmed, Usama A. Fahmy, Shadab Md, Gamal A. Mohamed, Sabrin R. M. Ibrahim, Basma G. Eid, Ashraf B. Abdel-Naim, Filippo Caraci
Summary: Impaired diabetic wound healing is a global concern for healthcare professionals. This study developed a fluoxetine ecofriendly nanoemulsion (FLX-EFNE) and demonstrated its significant efficacy in promoting wound healing in diabetic rats.
Article
Gastroenterology & Hepatology
Milka Lie Takejima, Maria Angelica Baron MagalhAes, Jurandir Marcondes Ribas Filho, Fernando Issamu Tabushi, Carlos Cesar Bof Bufon, Thayline Mylena Santana Camargo, Isabela Calixto Maluf, Osvaldo Malafaia
Summary: The study demonstrated that the Pinus sp cellulose membrane showed similar efficacy and safety in treating 3rd degree burns compared to the bacterial membrane. The cellulose membrane was more effective in promoting wound contraction and healing compared to the bacterial membrane.
ABCD-ARQUIVOS BRASILEIROS DE CIRURGIA DIGESTIVA-BRAZILIAN ARCHIVES OF DIGESTIVE SURGERY
(2021)
Article
Critical Care Medicine
Sigrid Blome-Eberwein, Adam Schwartz, Cassandra Pinataro, Patrick Pagella, Deborah Boorse, Christina Gogal
Summary: This study demonstrates that epidermal blister grafting is more effective than acellular treatment for 2a and 2b degree burns. The areas treated with epidermal grafting healed faster and with less erythema. Further research should focus on larger treatment areas and larger sample sizes.
Article
Biochemistry & Molecular Biology
Mohammed Z. Z. Nasrullah
Summary: Caffeic acid phenethyl ester-loaded PEG-PLGA nanoparticles have potent healing effects in diabetic wounds by accelerating wound closure and exhibiting antioxidant, anti-inflammatory, pro-collagen, and angiogenic activities.
Article
Plant Sciences
Qin Pan-Yue, Xu Ya-Jing, Zuo Xiang-Duo, Duan Jun-Hua, Qiu Bin, Li Xue-Fang, Li Jing-Ping, Yu Jie
Summary: This study aims to investigate the effects of PK extract on skin wound healing in diabetic rats and explore the regulatory mechanism of PK on wound microenvironment pollution, antioxidative stress signaling pathway, and latent injury of wound skin tissue.
JOURNAL OF ETHNOPHARMACOLOGY
(2022)
Article
Engineering, Biomedical
Antonio L. B. Pinheiro, Luiz G. P. Soares, Aline C. P. da Silva, Nicole R. S. Santos, Anna Paula L. T. da Silva, Bruno Luiz R. C. Neves, Amanda P. Soares, Marleny Elizabeth M. M. Gerbi, Jean Nunes dos Santos
Summary: The experimental results of treating complete tibial fractures with different phototherapy protocols indicate that the combination of MTA and laser therapy is more effective than LED therapy.
LASERS IN MEDICAL SCIENCE
(2021)
Article
Anatomy & Morphology
Rebeca C. M. Dantas, Vanessa S. N. Guimaraes, Renata O. de Souza, Ludmila F. Valverde, Manuela T. A. Vidal, Raphael L. R. Nogueira, Leonardo O. S. da Rocha, Giovana T. Araujo, Jean N. Dos Santos, Clarissa A. G. Rocha
Summary: In oral squamous cell carcinoma, activation of the Hedgehog pathway may be associated with epithelial-mesenchymal transition and cell proliferation. Positive GLi-1 expression was correlated with loss of membrane E-cadherin and beta-catenin expression, while GLi-1 was also associated with clinical staging. The study suggests that the HH pathway plays a role in regulating the mesenchymal phenotype in OSCC.
APPLIED IMMUNOHISTOCHEMISTRY & MOLECULAR MORPHOLOGY
(2021)
Article
Biochemistry & Molecular Biology
Felipe P. de Oliveira, Ana Carolina B. da C. Rodrigues, Emilly J. S. P. de Lima, Valdenizia R. Silva, Luciano de S. Santos, Talita A. da Anunciacao, Mateus L. Nogueira, Milena B. P. Soares, Rosane B. Dias, Clarissa A. Gurgel Rocha, Sergio Duvoisin Junior, Patricia M. Albuquerque, Emerson S. Lima, Jose F. C. Goncalves, Giovana A. Bataglion, Emmanoel Costa, Felipe M. A. da Silva, Hector H. F. Koolen, Daniel P. Bezerra
Summary: Aniba parviflora is an aromatic plant from the Amazon rainforest with commercial value in the perfumery industry, and is traditionally used in Brazilian folk medicine to treat snakebites. The essential oil extracted from its bark demonstrated significant growth inhibition on human hepatocellular carcinoma cells (HepG2) with minimal toxicity to non-cancerous cells.
CHEMISTRY & BIODIVERSITY
(2021)
Article
Anatomy & Morphology
Caroline B. Schlaepfer Sales, Vanessa S. N. Guimaraes, Ludmila F. Valverde, Rosane B. Dias, Raiza D. Freitas, Leonardo de Oliveira Siquara da Rocha, Malu Coelho de Miranda, Deise S. Vilas Boas, Ivan M. Goncalves Agra, Jean N. dos Santos, Clarissa A. Gurgel Rocha
Summary: Proteoglycans are involved in tumor development and may regulate the Hedgehog pathway in oral squamous cell carcinoma. Overexpression of GPC1 and GPC5, underexpression of GPC3 gene transcripts, and aberrant HH pathway activation were observed in OSCC. Positive staining of GPC1 and GPC5 were found in blood vessels adjacent to tumor islands.
APPLIED IMMUNOHISTOCHEMISTRY & MOLECULAR MORPHOLOGY
(2021)
Article
Anatomy & Morphology
Adna B. Ismerim, Iguaracyra B. de Oliveira Araujo, Flavia C. de Aquino Xavier, Clarissa A. G. Rocha, Claudia L. Macedo, Maria C. T. Cangussu, Valeria S. Freitas, Ricardo Della Coletta, Patricia R. Cury, Jean N. Santos
Summary: This study suggests that mast cells and PAR-2 protein may play important roles in the development and progression of malignant salivary gland cancers, regardless of myoepithelial content. The expression of myofibroblasts in tumors may be associated with cell mast density.
APPLIED IMMUNOHISTOCHEMISTRY & MOLECULAR MORPHOLOGY
(2021)
Article
Biochemistry & Molecular Biology
Patricia D. O. de Almeida, Gleyce dos Santos Barbosa Jobim, Caio Cesar dos Santos Ferreira, Lucas Rocha Bernardes, Rosane B. Dias, Caroline B. Schlaepfer Sales, Ludmila de F. Valverde, Clarissa A. G. Rocha, Milena B. P. Soares, Daniel P. Bezerra, Fernando de Carvalho da Silva, Mariana Filomena do Carmo Cardoso, Vitor Francisco Ferreira, Larissa F. Brito, Lirlandia Pires de Sousa, Marne C. de Vasconcellos, Emerson S. Lima
Summary: Quinones are plant-derived secondary metabolites with diverse pharmacological properties, including anti-tumor activity. A new naphthoquinone compound, CNFD, demonstrated cytotoxic effects on different tumor cell lines, particularly in MCF-7 breast adenocarcinoma cells. CNFD induced apoptosis through various mechanisms, such as gene expression modulation, activation of JNK and p38, and inhibition of ERK1/2 pathways.
CHEMICO-BIOLOGICAL INTERACTIONS
(2021)
Article
Medicine, Research & Experimental
Ana Carolina B. da C. Rodrigues, Larissa M. Bomfim, Sara P. Neves, Milena B. P. Soares, Rosane B. Dias, Ludmila F. Valverde, Clarissa A. Gurgel Rocha, Emmanoel V. Costa, Felipe M. A. da Silva, Waldireny C. Rocha, Hector H. F. Koolen, Daniel P. Bezerra
Summary: Tingenone (TG) and 22-hydroxytingenone (22-HTG) are quinonemethide triterpenes from the Amazonian plant Salacia impressifolia, showing potent cytotoxicity against AML HL-60 cell line through apoptosis pathway mediated by oxidative stress. These compounds are potential candidates for anti-AML therapy targeting thioredoxin.
BIOMEDICINE & PHARMACOTHERAPY
(2021)
Article
Energy & Fuels
Elias Ramos de Souza, Pamela Dias Rodrigues, Igor C. F. Sampaio, Edgard Bacic, Pedro J. L. Crugeira, Anais Couto Vasconcelos, Maira dos Santos Silva, Jacson N. dos Santos, Cristina M. Quintella, Antonio L. B. Pinheiro, Paulo Fernando de Almeida
Summary: This study describes the use of Produced Water Xanthan (PWX) as a chemical flooding metabolite for Enhanced Oil Recovery (EOR) and investigates its physico-chemical properties for application as an EOR agent. Comparisons are made between PWX and SigmaTM Xanthan Gum (SXG). The results show that PWX can be an effective EOR agent and has advantages in terms of environmental friendliness.
Article
Oncology
Sandra R. C. A. Fagnani, Susana C. P. S. de Oliveira, Juliana S. C. Monteiro, Fernando Jose Pires Sampaio, Pedro J. L. Crugeira, Jean N. dos Santos, Antonio L. B. Pinheiro
Summary: This study aimed to perform a histological evaluation in skin lesions caused by Leishmania braziliensis after PACT treatment using Laser associated with 1.9. dimethyl methylene blue. The results showed that PACT could reduce lymphoplasmacytic inflammation and increase tissue remodeling in leishmaniosis skin lesions.
PHOTODIAGNOSIS AND PHOTODYNAMIC THERAPY
(2022)
Article
Biochemistry & Molecular Biology
Alexandre F. C. Galvao, Morgana de S. Araujo, Valdenizia R. Silva, Luciano de S. Santos, Rosane B. Dias, Clarissa A. Gurgel Rocha, Milena B. P. Soares, Felipe M. A. da Silva, Hector H. F. Koolen, Gokhan Zengin, Emmanoel V. Costa, Daniel P. Bezerra
Summary: In this study, the cytotoxic and antitumor effects of the essential oil obtained from Guatteria olivacea leaves against liver cancer were evaluated. The essential oil exhibited potential in inhibiting liver cancer growth by inducing apoptosis and DNA fragmentation, without affecting reactive oxygen species levels.
Article
Immunology
Eduardo P. Amaral, Taylor W. Foreman, Sivaranjani Namasivayam, Kerry L. Hilligan, Keith D. Kauffman, Caio Cesar Barbosa Bomfim, Diego L. Costa, Beatriz Barreto-Duarte, Clarissa Gurgel-Rocha, Monique Freire Santana, Marcelo Cordeiro-Santos, Elsa Du Bruyn, Catherine Riou, Kate Aberman, Robert John Wilkinson, Daniel L. Barber, Katrin D. Mayer-Barber, Bruno B. Andrade, Alan Sher
Summary: In this study, the researchers found that GPX4, an antioxidant selenoenzyme, acts as an essential regulator of cellular necrosis and host resistance in tuberculosis caused by Mycobacterium tuberculosis (Mtb) infection. The study shows that Gpx4-deficient mice display increased lung necrosis and bacterial burdens, while mice overexpressing the enzyme have decreased bacterial loads and necrosis. The findings suggest that targeting the Gpx4/GSH axis could be a potential host-directed therapy for tuberculosis.
JOURNAL OF EXPERIMENTAL MEDICINE
(2022)
Review
Biochemistry & Molecular Biology
John Lenon de Souza Santos, Cecilia de Almeida Araujo, Clarissa Araujo Gurgel Rocha, Zaquer Suzana Munhoz Costa-Ferro, Bruno Solano de Freitas Souza
Summary: Autism spectrum disorders (ASD) are complex neurodevelopmental disorders that affect communication and social interactions. The understanding of the cellular and molecular mechanisms underlying ASD is still limited. Recent advancements in cellular reprogramming technology have allowed the generation of patient-specific cell models for mechanistic studies. Brain organoids derived from these cells have provided significant advances in the reproducibility of early human brain development, contributing to the study of ASD.
Article
Dentistry, Oral Surgery & Medicine
Bruno Cesar da Costa, Mauricio Rocha Dourado, Everton Freitas de Moraes, Luana Mari Panini, Amr Elseragy, Fabio Haach Teo, Gustavo Narvaes Guimaraes, Renato Assis Machado, Maija Risteli, Clarissa Araujo Gurgel Rocha, Livia Maris Ribeiro Paranaiba, Wilfredo Alejandro Gonzalez-Arriagada, Sabrina Daniela da Silva, Ana Lucia Carrinho Ayroza Rangel, Marcelo Rocha Marques, Tuula Salo, Ricardo D. Coletta
Summary: This study explored the prognostic impact of HSP47 in oral squamous cell carcinomas (OSCC) and the functional effects of its inhibition on OSCC cells. The results showed that HSP47 was overexpressed in OSCC samples and its overexpression was significantly associated with poor survival. Furthermore, the knockdown of HSP47 impaired the proliferation, migration, and invasion of OSCC cells.
JOURNAL OF ORAL PATHOLOGY & MEDICINE
(2023)
Article
Cell Biology
Leonardo de Oliveira da Rocha, Bruno Solano de Freitas Souza, Ricardo Della Coletta, Daniel W. Lambert, Clarissa A. Gurgel Rocha
Summary: Cell-in-cell (CIC) structures contribute to tumor aggressiveness and poor prognosis in oral squamous cell carcinoma (OSCC). In this study, spheroids obtained from OSCC and cancer-associated fibroblast (CAF) lines were used to investigate CIC structures. The spheroids showed the morphological aspects of the tumor microenvironment and CIC structures were found in both homotypic and heterotypic groups, predominantly in the proliferative zone of mixed spheroids. Complex cannibalism events were also observed. This model has the potential for further studies on CIC morphology, formation, and its relationship with tumor prognosis.
Article
Biochemistry & Molecular Biology
Emmanoel Costa, Cesar A. S. de Souza, Alexandre F. C. Galvao, Valdenizia R. Silva, Luciano de S. Santos, Rosane B. Dias, Clarissa A. Gurgel Rocha, Milena B. P. Soares, Felipe M. A. da Silva, Hector H. F. Koolen, Daniel P. Bezerra
Summary: In this study, the inhibitory effects of D. pycnastera leaf essential oil on the growth of liver cancer cells were investigated both in vitro and in vivo. The results showed that the oil exhibited cytotoxicity and also inhibited tumor mass growth in vivo.