期刊
PHOTOCHEMISTRY AND PHOTOBIOLOGY
卷 88, 期 3, 页码 717-720出版社
WILEY
DOI: 10.1111/j.1751-1097.2012.01106.x
关键词
-
资金
- National Cancer Institute [CA23378]
- National Institutes of Health [T32 CA009531]
The cell-permeable anthracene analog diethyl-3-3'-(9,10-anthracenediyl)bis acrylate (DADB) was recently identified as a highly selective probe for singlet oxygen (1O2). Now, we show that DADB can be used to monitor 1O2 formation in cell culture during photodynamic therapy. An atypical property of DADB is that fluorescence emission is decreased upon oxidation. Using photosensitizers that target specific organelles, we determined that DADB could detect 1O2 whether formed in ER, mitochondria or lysosomes. DADB fluorescence was not, however, significantly altered when the photosensitizing agent was the palladium bacteriopheophorbide termed WST11, an agent reported to produce mainly oxygen radicals upon irradiation in an aqueous environment, whereas singlet oxygen was formed in organic solvents.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据