Article
Toxicology
Congying Zheng, Sergey Shaposhnikov, Andrew Collins, Gunnar Brunborg, Florin Oancea, Frederik-Jan Van Schooten, Roger Godschalk
Summary: DNA repair is crucial for maintaining genomic stability, and can be evaluated through comet assay-based approaches such as the cellular repair assay and the in vitro repair assay. However, a direct comparison of both assays in multiple cell lines showed no significant relationship between them. The in vitro repair assay correlated with the background level of DNA damage in the test cells and indicated that base excision repair is inducible. These findings suggest that these two assays measure different endpoints of DNA repair and should be considered complementary.
ARCHIVES OF TOXICOLOGY
(2023)
Article
Toxicology
Ezgi Eyluel Bankoglu, Carolin Schuele, Helga Stopper
Summary: The comet assay is widely used in basic research, genotoxicity testing, and human biomonitoring. The study found that DNA damage exceeding 20-30% tail DNA caused more than 50% of cells to die, with etoposide causing slightly more cell death than H2O2 or MMS. Cells showed some repair capacity for DNA damage within a few hours after substance removal.
ARCHIVES OF TOXICOLOGY
(2021)
Article
Toxicology
Ezgi Eyluel Bankoglu, Franzisca Stipp, Johanna Gerber, Florian Seyfried, August Heidland, Udo Bahner, Helga Stopper
Summary: The comet assay is a widely used method for assessing DNA damage and repair activity in various samples, with cryopreserved blood preparations found to be suitable for such analysis. The study also revealed that ex vivo hydrogen peroxide challenge had optimal effects in PBMCs, while repair comet assays worked well in fresh PBMCs.
ARCHIVES OF TOXICOLOGY
(2021)
Article
Cell Biology
Congying Zheng, Andrew Collins, Gunnar Brunborg, Frederik-Jan van Schooten, Anne Lene Nordengen, Sergey Shaposhnikov, Roger Godschalk
Summary: DNA repair plays a crucial role in cancer development, progression, prognosis, and response to therapy. In this study, a cellular repair assay based on the Fpg-modified comet assay was used to evaluate DNA repair kinetics. The removal of oxidized nucleobases over time was analyzed in peripheral blood mononuclear cells (PBMCs) and 8 cell lines. DNA damage was induced by exposure to Ro19-8022 plus visible light or potassium bromate (KBrO3). The results showed a fast initial removal of Fpg-sensitive sites followed by slower removal over time.
CELL BIOLOGY AND TOXICOLOGY
(2023)
Article
Chemistry, Multidisciplinary
Iman A. Mohammed A. Ali, Hazim Ismail AL-Ahmed, Ali Ben Ahmed
Summary: The synthesis of selenium nanoparticles (Se NPs) from the aqueous extract of Withania somnifera roots has been confirmed. The Se NPs have shown promising potential in improving sperm efficacy and antioxidant defense mechanisms.
APPLIED SCIENCES-BASEL
(2023)
Article
Genetics & Heredity
Jing Ge, Le P. Ngo, Simran Kaushal, Ian J. Tay, Elina Thadhani, Jennifer E. Kay, Patrizia Mazzucato, Danielle N. Chow, Jessica L. Fessler, David M. Weingeist, Robert W. Sobol, Leona D. Samson, Scott R. Floyd, Bevin P. Engelward
Summary: DNA damage is directly linked to aging, cancer, and other diseases, and cells have evolved highly conserved DNA repair pathways to counteract its deleterious effects. The CometChip platform offers increased throughput and sensitivity for parallel analysis of multiple DNA repair activities, expanding the utility of the comet assay for precise, high-throughput assessment of repair deficiencies in different pathways.
Article
Biochemistry & Molecular Biology
Sanja Ercegovic Razic, Nevenka Kopjar, Vilena Kasuba, Zenun Skenderi, Jadranka Akalovic, Jasna Hrenovic
Summary: The production and processing of cowhide have long used chrome tanning, but the waste generated from chromium has negative effects on health and the environment. This study investigated the effects of different tanning agents on DNA damage in HepG2 cells and found that vegetable tanning resulted in the highest DNA instability. These preliminary results provide insights into the genotoxic potential of leather processing methods and can guide future experiments.
Article
Chemistry, Multidisciplinary
Monica Reynoso-Silva, Carlos Alvarez-Moya, Rafael Ramirez-Velasco, Alexis Gerardo Samano-Leon, Erandi Arvizu-Hernandez, Hugo Castaneda-Vasquez, Mario Alberto Ruiz-Lopez
Summary: A new approach for genetic damage assessment using the comet assay is proposed based on specific parameters which can improve the detection of genetic damage associated with certain genotoxic agents. The study shows significant differences in tail length and migration groups among genotoxic agents, indicating a specific chemical interaction on the DNA.
APPLIED SCIENCES-BASEL
(2021)
Article
Environmental Sciences
Mahara Valverde, Adriana Granados, Mirta Milic, Marcello Ceppi, Leticia Sollano, Stefano Bonassi, Emilio Rojas
Summary: DNA damage in newborn children's cord blood cells from Mexico City is correlated with the DNA damage in their mothers, as well as the environmental exposure and complications during pregnancy. Mothers living in the southern part of the city have double DNA damage compared to those living in the northern part. This study highlights the importance of antioxidants during pregnancy and the role of the placental barrier in protecting newborns from DNA-damaging effects of pollution.
Article
Microbiology
Carlos Verdu, Patricia Perez-Arnaiz, Ana Peropadre, Jose Berenguer, Mario Mencia
Summary: This study demonstrates the important role of DNA primase-polymerases (Ppol) in DNA repair and damage tolerance in both bacteria and eukaryotes. The research indicates that Ppol plays a key role in genome stability by balancing the activity of AddAB in T. thermophilus HB27.
FRONTIERS IN MICROBIOLOGY
(2022)
Review
Biotechnology & Applied Microbiology
Tong-shuai Wang, Mathuros Ruchirawat, Panida Narasumrit, Zhao-lin Xia, William W. Au
Summary: Combinations of genetic and environmental factors play a significant role in the development of human diseases, including cancer. DNA repair is crucial in determining the outcomes of DNA damage and its impact on cellular consequences. Challenge assays can amplify variations in DNA repair capacity between individuals, providing more precise disease prevention and intervention methods.
MUTATION RESEARCH-REVIEWS IN MUTATION RESEARCH
(2022)
Article
Endocrinology & Metabolism
Esra Nur Tola, Mevlut Bucak, Atahan Togay, Pinar Aslan Kosar
Summary: This study aimed to evaluate the relation between gestational diabetes mellitus (GDM) and maternal and/or fetal DNA integrity. The results showed that GDM group had higher body mass index (BMI), weight gain during pregnancy, glycemic means, and fetal weight compared to the control group. The number of large-for-gestational-age (LGA) infants was also significantly higher in the GDM group. Placental DNA damage was associated with GDM, and placental cells were more vulnerable to DNA damage compared to fetal blood cells.
GYNECOLOGICAL ENDOCRINOLOGY
(2022)
Article
Biochemistry & Molecular Biology
Mohammad Hashim, Hussain Arif, Baby Tabassum, Amin Arif, Ahmed A. Rehman, Shahnawaz Rehman, Rehnuma Khanam, Bushra Khan, Arif Hussain, Jameel Barnawi, Faris J. Tayeb, Naseh Algehainy, Faisal H. Altemani, Rashid Mir, Fahad M. Almutairi, Mohammad Fahad Ullah, Imadeldin Elfaki, Mohammad Rehan Ajmal
Summary: This article investigates the protective ability of Catharanthus roseus (CR) extract on oxidative stress in the kidney and liver of rats exposed to Cadmium (Cd). The study found that Cd exposure resulted in oxidative stress and damage in the kidney and liver, while the CR extract showed potential antioxidant activity and reduced the level of oxidative damage.
Article
Medicine, Research & Experimental
Arun Kumar, Sandeep Choudhary, Somesh Kumar, Jawahar S. Adhikari, Seema Kapoor, Nabo K. Chaudhury
Summary: Melatonin plays a role in recovering the hematopoietic system of irradiated mice by inducing G-CSF mediated radioprotection and related mechanisms. Different doses of melatonin, with 20mg/kg as the optimal dose, effectively reduce radiation-induced DNA damage and cell death.
Article
Public, Environmental & Occupational Health
Vesna Benkovic, Nada Orsolic, Anica Horvat Knezevic, Nikola Borojevic, Gordana Brozovic, Mirta Milic
Summary: This study investigated the effects of volatile anaesthetics and ionising radiation on DNA damage in kidney cells. The results showed that exposure to volatile anaesthetics alone increased DNA damage, while combined exposure to anaesthetics and 1 Gy or 2 Gy radiation had weaker damaging effects on DNA. Sevoflurane demonstrated the most severe damage in the combined exposure.
ARHIV ZA HIGIJENU RADA I TOKSIKOLOGIJU-ARCHIVES OF INDUSTRIAL HYGIENE AND TOXICOLOGY
(2022)
Article
Nutrition & Dietetics
Murat Boyacioglu, Cavit Kum, Selim Sekkin, Hande Sultan Yalinkilinc, Hamdi Avci, Erkmen Tugrul Epikmen, Umit Karademir
CLINICAL NUTRITION
(2016)
Article
Veterinary Sciences
Hamdi Avci, Selim Sekkin, Murat Boyacioglu, Hasan Aksit, Recai Tunca, E. Tugrul Epikmen, S. Serap Birincioglu
KAFKAS UNIVERSITESI VETERINER FAKULTESI DERGISI
(2016)
Article
Veterinary Sciences
U. Karademir, H. Erdogan, M. Boyacioglu, C. Kum, S. Sekkin, M. Bilgen
NEW ZEALAND VETERINARY JOURNAL
(2016)
Article
Pathology
H. Avci, E. T. Epikmen, E. Ipek, R. Tunca, S. S. Birincioglu, H. Aksit, S. Sekkin, A. N. Akkoc, M. Boyacioglu
EXPERIMENTAL AND TOXICOLOGIC PATHOLOGY
(2017)
Article
Reproductive Biology
Zahid Naseer, Ejaz Ahmad, Hande Sultan Sahiner, Erkmen Tugrul Epikmen, Muhammad Fiaz, Muhamad Rizwan Yousuf, Shahzad Akbar Khan, Ilker Serin, Ahmet Ceylan, Melih Aksoy
Article
Biochemistry & Molecular Biology
Murat Boyacioglu, Ozay Gules, Hande Sultan Sahiner
Summary: The study found that Sodium selenite (SS) may have a protective effect against 4-nonylphenol (4-NP)-induced hepato-nephrotoxicity in rats by significantly reducing DNA damage, improving antioxidant/oxidant status in liver and kidney tissues, and alleviating histological changes.
BIOLOGICAL TRACE ELEMENT RESEARCH
(2021)
Article
Agriculture, Dairy & Animal Science
Omer Sevim, Onur Tatli, Eren Kuter, Umair Ahsan, Ehsan Karimiyan Khamseh, Artun Reman, Ozge Sayin Ozdemir, Mehmet Kaya, Hande Sultan Sahiner, Bekir Hakan Koksal, Ozcan Cengiz
Summary: The study found that supplemental humate in feed and/or litter did not alleviate footpad dermatitis (FPD) in broilers fed diets based on barley. Additionally, the presence of FPD lesions increased malondialdehyde (MDA) and superoxide dismutase (SOD) levels in the footpad tissues of broilers.
TROPICAL ANIMAL HEALTH AND PRODUCTION
(2021)
Article
Pharmacology & Pharmacy
Cengiz Gokbulut, Veli Y. Cirak, Suleyman Aypak, Hamdi Avci, Selim Sekkin, Hande S. Yalinkilinc, Buket Boga, Erol Ayaz
Summary: The plasma concentration profiles of RBZ in goats are strongly correlated with those achieved in different target tissues or fluids, reflecting the amount of drug taken up by parasites.
JOURNAL OF VETERINARY PHARMACOLOGY AND THERAPEUTICS
(2021)
Article
Pharmacology & Pharmacy
Metin Deniz Karakoc, Selim Sekkin
Summary: The study found that oleuropein can reduce DNA damage and levels of serum TNF-alpha and IL-6, improve some hematologic and biochemical parameters deteriorated by antineoplastic drugs. Moreover, it increased antioxidant levels (GSH, SOD, and CAT) and decreased MDA levels in heart, kidney, and liver tissues.
TURKISH JOURNAL OF PHARMACEUTICAL SCIENCES
(2021)
Article
Agriculture, Dairy & Animal Science
O. Sevim, U. Ahsan, O. Tatli, E. Kuter, E. Karimiyan Khamseh, A. Reman Temiz, O. Sayin Ozdemir, A. K. Aydin, H. S. Sahiner, M. Avcioglu, E. Koc Yildirim
Summary: The study found that high stocking density and dietary nano-Se may not affect organ index, stress indicators, and immune function. However, dietary nano-Se can reduce the potential negative impact of high stocking density on serum interferon-gamma. High stocking density increased DNA damage in lymphocytes, but dietary nano-Se had no significant effect on it.
EUROPEAN POULTRY SCIENCE
(2021)
Article
Veterinary Sciences
Funda Kiral, Selim Sekkin, Serdar Pasa, Hatice Ertabaklar, Pinar Alkim Ulutas, Gamze Sevri Ekren Asici
Summary: This study investigated DNA and protein damage in dogs infected with Leishmania infantum, finding higher levels of plasma protein carbonyl and nitrotyrosine in infected dogs compared to healthy dogs, along with significantly increased plasma malondialdehyde. Antioxidant capacity was lower in infected dogs. The parasites caused oxidative stress through protein and DNA damage, leading to decreased antioxidant capacity.
MEDYCYNA WETERYNARYJNA-VETERINARY MEDICINE-SCIENCE AND PRACTICE
(2021)
Article
Pharmacology & Pharmacy
Hande Sultan Sahiner, Cavit Kum
Summary: The study investigated the pharmacokinetic profile of co-administration of tulathromycin with rifampicin in healthy male goats. Results showed that rifampicin administration may alter several pharmacokinetic parameters of tulathromycin in goats.
JOURNAL OF VETERINARY PHARMACOLOGY AND THERAPEUTICS
(2021)
Article
Public, Environmental & Occupational Health
Serdal Ogut, Hossein Asgarpour, Selim Sekkin
PUERTO RICO HEALTH SCIENCES JOURNAL
(2019)
