Article
Cell Biology
Lujia Feng, Yantao Wei, Yimeng Sun, Linbin Zhou, Shaowei Bi, Weirong Chen, Wu Xiang
Summary: Cataracts are a leading cause of blindness worldwide, with age being a major risk factor. The exact mechanisms of cataractogenesis are still unclear. A recent study found that microRNA-34a (MIR34A) is involved in cataract development, but the underlying pathogenesis is unknown. This study showed that hexokinase 1 (HK1) is a target gene of MIR34A and demonstrated that MIR34A modulates lens epithelial cell apoptosis and cataract development through the HK1/caspase 3 signaling pathway.
Review
Cell Biology
Lisa Brennan, M. Joseph Costello, J. Fielding Hejtmancik, A. Sue Menko, S. Amer Riazuddin, Alan Shiels, Marc Kantorow
Summary: Recent studies have shown that autophagy plays a crucial role in achieving the mature structure, homeostasis, and transparency of the lens. Various experiments using different model systems have found autophagy structures in lens cells, indicating its importance in differentiation. It has also been observed that autophagy is required for lens homeostasis and transparency, as mutations in autophagy proteins can lead to cataract formation.
Article
Ophthalmology
Tiantian Qi, Ruihua Jing, Bo Ma, Conghui Hu, Chan Wen, Yongping Shao, Cheng Pei
Summary: This study reveals that the downregulation of RNF157 contributes to age-related cataract by upregulating p53, suggesting that modulation of RNF157 expression may serve as a potential therapeutic strategy for cataracts.
INVESTIGATIVE OPHTHALMOLOGY & VISUAL SCIENCE
(2022)
Article
Biochemistry & Molecular Biology
Shanhe Liu, Dongmei Su, Zhaoyi Sun, Lina Guan, Zhongying Wang, Gaobo Zhang, Guiqian Zheng, Tingsong Cui, Xu Ma, Shanshan Hu
Summary: This study found that the MST2/YAP1/GLUT1 pathway plays a critical role in the development of age-related cataract and apoptosis of lens epithelial cells (LECs), suggesting potential for targeted inhibitors to prevent or treat cataracts.
ARCHIVES OF BIOCHEMISTRY AND BIOPHYSICS
(2022)
Article
Cell Biology
Minglei Zhao, Tingfang Mei, Bizhi Shang, Bin Zou, Qing Lian, Wenchang Xu, Keling Wu, Yuhua Lai, Chujun Liu, Lai Wei, Jie Zhu, Kang Zhang, Yizhi Liu, Ling Zhao
Summary: The study demonstrates that a mouse model carrying Lss G589S mutation can recapitulate human congenital cataract and indicates the critical role of LSS in lens development. The mutation leads to opacity in eye lens formation, disrupted lens differentiation, and downregulation of cholesterol synthesis signaling pathways.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2021)
Article
Biochemistry & Molecular Biology
Yuefang Zhou, Thomas M. Bennett, Alan Shiels
Summary: TRPM3 gene mutation is associated with early-onset cataracts, and our study on mice with this mutation provides insights into ocular phenotypes.
Article
Multidisciplinary Sciences
Vikash Chandra, Hazem Ibrahim, Clementine Halliez, Rashmi B. Prasad, Federica Vecchio, Om Prakash Dwivedi, Jouni Kvist, Diego Balboa, Jonna Saarimaki-Vire, Hossam Montaser, Tom Barsby, Vaino Lithovius, Isabella Artner, Swetha Gopalakrishnan, Leif Groop, Roberto Mallone, Decio L. Eizirik, Timo Otonkoski
Summary: The TYK2 gene is crucial in the development of type 1 diabetes. In this study, the researchers demonstrate that TYK2 regulates the development of beta-cells and inhibiting TYK2 in the islets enhances their survival against CD8(+) T-cell cytotoxicity. This finding highlights TYK2 as a potential therapeutic target to halt the progression of type 1 diabetes.
NATURE COMMUNICATIONS
(2022)
Article
Cell Biology
Yuan Xiao, Jia-Wen Xiang, Qian Gao, Yue-Yue Bai, Zhao-Xia Huang, Xiao-Hui Hu, Ling Wang, David Wan-Cheng Li
Summary: Research has shown that Mab21L1 promotes survival of lens epithelial cells by regulating the expression of αB-crystallin and suppressing the ATR/CHK1/p53 pathway. This finding contributes to a better understanding of the functional mechanism of Mab21L1.
Article
Biochemistry & Molecular Biology
Wenyi Zhang, Anran Wu, Guowei Zhang, Xuemeng Ding, Lihua Kang, Tianqiu Zhou, Min Ji, Huaijin Guan
Summary: Oxidative damage-triggered apoptosis in lens epithelial cells is a main risk factor in age-related cataract pathogenesis. Ku70 and its related E3 ubiquitin ligase play a role in lens epithelial cell apoptosis. Ku70 levels were lower in cataracts and Emory mice, and oxidative stress decreased Ku70 expression through ubiquitination. Parkin, an E3 ubiquitin ligase, interacted with Ku70 and promoted its ubiquitination and degradation. Ubiquitinated Ku70 was regulated by ubiquitin-proteasome, autophagy-lysosome, and mitophagy pathways. Ku70 overexpression protected cells from apoptosis, while Ku70 silencing had the opposite effect. Non-ubiquitinatable Ku70 mutant maintained its anti-apoptosis ability, in contrast to wild-type Ku70. Ku70 may also facilitate mitochondrial fusion. Overall, this study demonstrated the role of Parkin-mediated Ku70 ubiquitination in oxidative stress-induced lens epithelial cell apoptosis, providing potential targets for age-related cataract treatment.
Article
Cell Biology
Matthieu Duot, Roselyne Viel, Justine Viet, Catherine Le Goff-Gaillard, Luc Paillard, Salil A. Lachke, Carole Gautier-Courteille, David Reboutier
Summary: This study successfully generated lens organoids that mimic the properties and organization of the lens tissue, providing a new research tool for studying lens biology and cataracts, as well as for drug screening.
Article
Biotechnology & Applied Microbiology
Gerard Boix-Lemonche, Richard M. Nagymihaly, Xhevat Lumi, Goran Petrovski
Summary: This research demonstrates the trilineage differentiation potential of human lens cells and reveals the risks of complications during cataract surgery, such as calcification and osteogenic differentiation.
FRONTIERS IN BIOENGINEERING AND BIOTECHNOLOGY
(2023)
Article
Ophthalmology
Mason Posner, Kelly L. Murray, Brandon Andrew, Stuart Brdicka, Alexis Roberts, Kirstan Franklin, Adil Hussen, Taylor Kaye, Emmaline Kepp, Mathew S. McDonald, Tyler Snodgrass, Keith Zientek, Larry L. David
Summary: Alpha-crystallin small heat shock proteins play a role in maintaining the transparency and refractive properties of the eye lens, and their absence can lead to lens defects. In this study, zebrafish lines with mutations in the alpha-crystallin genes were generated using CRISPR gene editing. Loss of alpha A-crystallin resulted in various lens defects, while loss of alpha Ba- and alpha Bb-crystallin did not cause noticeable lens abnormalities. These findings suggest that alpha A-crystallin plays a role in lens development, but its loss is not essential. The study also highlights the need for further research on the molecular mechanisms behind lens defects and the impact of alpha A-crystallin loss in aging zebrafish lenses.
EXPERIMENTAL EYE RESEARCH
(2023)
Article
Medicine, Research & Experimental
Xiao Li, Miaomiao Sun, Anran Cheng, Guangying Zheng
Summary: DC is a major ocular complication of diabetes, characterized by EMT of LECs. GAS5 and miR-204-3p are involved in the pathogenic mechanism through regulating migration and EMT of LECs induced by HG. The dysregulation of GAS5 impacts lens epithelial cell migration and EMT under HG conditions via the miR-204-3p/TGFBR1 axis.
LABORATORY INVESTIGATION
(2022)
Review
Cell Biology
Wojciech J. Szlachcic, Natalia Ziojla, Dorota K. Kizewska, Marcelina Kempa, Malgorzata Borowiak
Summary: Insulin plays a crucial role in maintaining blood glucose homeostasis, and chronic inability to do so can lead to diabetes and organ damage. Advancements in technologies like single-cell RNA sequencing have allowed for a deeper understanding of pancreatic islet cell development and their role in diabetes, offering insights for better treatment strategies.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2021)
Article
Medicine, General & Internal
Miaomiao Sun, Ke Li, Xiao Li, Huajun Wang, Li Li, Guangying Zheng
Summary: This study identified the competitive inhibition of miR-29b expression by lncRNA TUG1, leading to increased Smac release and promotion of lens epithelial cell apoptosis in age-related cataracts. This mechanism contributes to the formation of ARC and suggests a new molecular pathway for regulating ARC development.
CHINESE MEDICAL JOURNAL
(2023)
Article
Ophthalmology
Nathan G. Lambert, Hanan ElShelmani, Malkit K. Singh, Fiona C. Mansergh, Michael A. Wride, Maximilian Padilla, David Keegan, Ruth E. Hogg, Balamurali K. Ambati
PROGRESS IN RETINAL AND EYE RESEARCH
(2016)
Article
Developmental Biology
Fiona C. Mansergh, Carl S. Daly, Anna L. Hurley, Michael A. Wride, Susan M. Hunter, Martin J. Evans
BMC DEVELOPMENTAL BIOLOGY
(2009)
Article
Ophthalmology
Jenny C. Geatrell, Peng Mui (Iryn) Gan, Fiona C. Mansergh, Lilian Kisiswa, Miguel Jarrin, Llinos A. Williams, Martin J. Evans, Mike E. Boulton, Michael A. Wride
EXPERIMENTAL EYE RESEARCH
(2009)
Article
Ophthalmology
Nicola Goodall, Lilian Kisiswa, Ankush Prashar, Stuart Faulkner, Pawel Tokarczuk, Krish Singh, Jonathan T. Erichsen, Jez Guggenheim, Willi Halfter, Michael A. Wride
EXPERIMENTAL EYE RESEARCH
(2009)
Article
Ophthalmology
Lilian Kisiswa, Julie Albon, James E. Morgan, Michael A. Wride
EXPERIMENTAL EYE RESEARCH
(2010)
Review
Ophthalmology
Lilian Kisiswa, Adrian G. Dervan, Julie Albon, James E. Morgan, Michael A. Wride
OPHTHALMIC RESEARCH
(2010)
Article
Pediatrics
Reshma Doodnath, Adrian Dervan, Michael A. Wride, Prem Puri
PEDIATRIC SURGERY INTERNATIONAL
(2010)
Article
Pediatrics
R. Doodnath, M. Wride, P. Puri
PEDIATRIC SURGERY INTERNATIONAL
(2012)
Editorial Material
Biology
I. Michael Wormstone, Michael A. Wride
PHILOSOPHICAL TRANSACTIONS OF THE ROYAL SOCIETY B-BIOLOGICAL SCIENCES
(2011)
Article
Acoustics
Despina Bazou, Roisin Kearney, Fiona Mansergh, Celine Bourdon, Jane Farrar, Michael Wride
ULTRASOUND IN MEDICINE AND BIOLOGY
(2011)
Editorial Material
Biochemistry & Molecular Biology
Ralf Dahm, Jake Rowan Byrne, Daniel Rogers, Michael A. Wride
Article
Education & Educational Research
Philip Franses, Mike Wride
HIGHER EDUCATION SKILLS AND WORK-BASED LEARNING
(2015)
Article
Biochemistry & Molecular Biology
Miguel Jarrin, Fiona C. Mansergh, Michael E. Boulton, Lena Gunhaga, Michael A. Wride
Article
Ophthalmology
Kate A. Powell, Jennifer R. Davies, Elaine Taylor, Michael A. Wride, Marcela Votruba
INVESTIGATIVE OPHTHALMOLOGY & VISUAL SCIENCE
(2011)