Article
Infectious Diseases
Emma L. Sweeney, Catriona S. Bradshaw, Gerald L. Murray, David M. Whiley
Summary: Individualized treatment of Mycoplasma genitalium infection should focus on fluoroquinolone resistance mutations to guide antimicrobial treatment and post-treatment microbial cure. The development of molecular tests that can detect both wild-type sequences and common ParC mutations, such as Ser83Ile, could greatly improve antimicrobial selection and patient care.
LANCET INFECTIOUS DISEASES
(2022)
Article
Infectious Diseases
Emma L. Sweeney, Catriona S. Bradshaw, Gerald L. Murray, David M. Whiley
Summary: Mycoplasma genitalium poses an emerging global health threat with increasing antimicrobial resistance. The use of individualised treatment approaches has been successful for macrolides, but the rise of fluoroquinolone resistance and lack of alternative treatment options complicate treatment. This article discusses the available literature data and highlights issues surrounding individualised treatment using fluoroquinolones, including the hesitance to consider ParC fluoroquinolone resistance mutations in guiding antimicrobial treatments. The authors propose the use of diagnostics that focus on the absence of resistance mutations to inform microbial cure following fluoroquinolone antimicrobials, with Australian data supporting this approach. Development of molecular tests detecting both wild-type and the most common ParC mutation could greatly improve antimicrobial selection, individualise tests of cure, and aid in the care of patients with M genitalium infection.
LANCET INFECTIOUS DISEASES
(2022)
Article
Public, Environmental & Occupational Health
Catherine E. Oldenburg, Armin Hinterwirth, Millogo Ourohire, Clarisse Dah, Moussa Ouedraogo, Ali Sie, Valentin Boudo, Cindi Chen, Kevin Ruder, Lina Zhong, Elodie Lebas, Fanice Nyatigo, Benjamin F. Arnold, Kieran S. O'Brien, Thuy Doan
Summary: This study compared the effects of single-dose azithromycin and a 7-day course of amoxicillin on children with uncomplicated severe acute malnutrition (SAM), and found no difference in the impact on antibiotic resistance genes 8 weeks after treatment.
AMERICAN JOURNAL OF TROPICAL MEDICINE AND HYGIENE
(2022)
Article
Multidisciplinary Sciences
Raphael Scheibenpflug, Markus Obermueller, Gottfried Reznicek, Ortrun Neuper, Wolfgang W. Lamm, Markus Raderer, Heimo Lagler
Summary: The study found that azithromycin levels in plasma remained stable in MALT-lymphoma patients, with poorer treatment response seen in patients with higher concentrations in PMN. Age, weight, and body-mass-index did not significantly influence plasma levels, and should not be considered in dosage finding.
SCIENTIFIC REPORTS
(2021)
Article
Chemistry, Multidisciplinary
Muhammad Sohail Arshad, Aleema Tehreem Zahra, Saman Zafar, Hussain Zaman, Ambreen Akhtar, Muhammad Mazhar Ayaz, Israfil Kucuk, Mohammed Maniruzzaman, Ming-Wei Chang, Zeeshan Ahmad
Summary: This study aimed to fabricate polymeric microneedles loaded with macrolides for the treatment of infected wounds. The microneedles showed acceptable physical and chemical characteristics, with efficient drug release and antibacterial effects. Treatment with azithromycin-loaded microneedle patches resulted in complete wound healing and tissue regeneration after 5 days.
PHARMACEUTICAL RESEARCH
(2021)
Article
Pharmacology & Pharmacy
Muradi Albanji, Samah Alshehri, Khalid Eljaaly
Summary: This study found a significantly higher increase in serum trough levels of valproate after coadministration of erythromycin/clarithromycin compared to azithromycin. Clinicians should consider avoiding concurrent use of erythromycin and clarithromycin with valproate, if possible, or closely monitor valproate levels and reduce the dosage.
SAUDI PHARMACEUTICAL JOURNAL
(2022)
Article
Chemistry, Analytical
Sergii Okorochenkov, Zdenek Skrott, Dusana Majera, Martin Porubsky, Martin Mistrik, Jan Hlavac
Summary: A new fluorescent dye BODIPYAZD-1 is introduced in this study, able to selectively stain autolysosomes and distinguish them from lysosomes and autophagosomes. This compound has excellent fluorescent properties, is not phototoxic, and is compatible with live-cell and fixed-cell microscopy.
SENSORS AND ACTUATORS B-CHEMICAL
(2022)
Article
Microbiology
Anna Carrera-Salinas, Aida Gonzalez-Diaz, Rachel L. Ehrlich, Damaris Berbel, Fe Tubau, Xavier Pomares, Junkal Garmendia, M. Angeles Dominguez, Carmen Ardanuy, Daniel Huertas, Alicia Marin, Conchita Monton, Joshua Chang Mell, Salud Santos, Sara Marti
Summary: The immunomodulatory properties of azithromycin reduce the frequency of exacerbations and improve the quality of life of COPD patients. However, long-term administration may alter the respiratory microbiota, such as Haemophilus influenzae, an opportunistic respiratory colonizing bacteria that play an important role in exacerbations. This study contributes to a better understanding of COPD progression by characterizing the clinical evolution of H. influenzae in a cohort of patients with prolonged azithromycin treatment. The emergence of macrolide resistance during the first months, combined with the role of Haemophilus parainfluenzae as a reservoir and source of resistance dissemination, is a cause for concern that may lead to therapeutic failure. Furthermore, genetic variations in cell wall and inorganic ion metabolism coding genes likely favor bacterial adaptation to host selective pressures. Therefore, the bacterial pathoadaptive evolution in these severe COPD patients raise our awareness of the possible spread of macrolide resistance and selection of host-adapted clones.
MICROBIOLOGY SPECTRUM
(2023)
Editorial Material
Emergency Medicine
Steven Lai, Kellie Kitamura
Summary: This article is part of a regular series on emerging infections from CDC and EMERGEncy ID NET, providing important infectious disease public health information relevant to emergency physicians. The goal is to advance knowledge about communicable diseases in emergency medicine and promote collaboration between clinical medicine and public health agencies.
ANNALS OF EMERGENCY MEDICINE
(2021)
Article
Ophthalmology
Gurbani Kaur, Travis K. Redd, Gerami D. Seitzman
Summary: Clinicians who regularly treat ocular rosacea lack consensus on the optimal initial systemic treatment dosage and understanding of the treatment's mechanism of action.
Article
Chemistry, Medicinal
Shuliang Song, Qiang Wei, Ke Wang, Qiong Yang, Yu Wang, Aiguo Ji, Guanjun Chen
Summary: This study used fluorescently labeled PM to investigate its distribution and pharmacokinetics in vivo. The results showed that FITC-PM had high stability and tended to be distributed in the kidney, liver, and at lower concentrations in the heart, spleen, and lungs after tail vein injection. Pharmacokinetic analysis revealed appropriate elimination rate constant, half-life time, and clearance rate for FITC-PM. The studies of pharmacokinetics and tissue distribution provided basic information that can be used to further clarify the pharmacodynamic mechanisms of PM.
Review
Infectious Diseases
Lauri Ivaska, Alex-Mikael Barkoff, Jussi Mertsola, Qiushui He
Summary: This review summarizes the current understanding of the epidemiology, resistance mechanisms, and clinical implications of macrolide resistance in Bordetella pertussis. China has seen a high prevalence of macrolide-resistant B. pertussis, while sporadic cases have been reported in other parts of the world.
Article
Chemistry, Medicinal
Liping Dong, Wenjuan Liu, Xiaoyuan Zhao, Feng Yu, Yungen Xu, Mengxiang Su
Summary: This study synthesized a novel limonin derivative, HY-071085, with strong anti-inflammatory and analgesic activity. The pharmacokinetics, bioavailability, distribution, and excretion of HY-071085 were evaluated in rats and beagle dogs. The results showed nonlinear dynamic characteristics and gender differences in the pharmacokinetics of HY-071085, as well as tissue distribution and excretion patterns.
Article
Plant Sciences
Vladimir Skalicky, Ioanna Antoniadi, Ales Pencik, Ivo Chamrad, Rene Lenobel, Martin F. Kubes, Marek Zatloukal, Asta Zukauskaite, Miroslav Strnad, Karin Ljung, Ondrej Novak
Summary: Auxin and cytokinin show subcellular distribution in Arabidopsis cells, forming hormone gradients. This finding provides important insights into the mechanisms of plant growth and development.
Article
Biochemical Research Methods
Florin C. Walter, Oliver Stegle, Britta Velten
Summary: This study proposes a probabilistic factor model called FISHFactor that can take single molecule resolution data as input and explicitly model and account for its nature. Compared to existing methods, FISHFactor shows more accurate results on simulated data and can be applied to large datasets. Additionally, FISHFactor can identify major subcellular expression patterns and spatial gene clusters in a data-driven manner.
Article
Pharmacology & Pharmacy
Mihaela Peric, Dijana Pesic, Sulejman Alihodzic, Andrea Fajdetic, Esperanza Herreros, Francisco Javier Gamo, Inigo Angulo-Barturen, Maria Belen Jimenez-Diaz, Santiago Ferrer-Bazaga, Maria S. Martinez, Domingo Gargallo-Viola, Amanda Mathis, Albane Kessler, Mihailo Banjanac, Jasna Padovan, Vlatka Bencetic Mihaljevic, Vesna Munic Kos, Mirjana Bukvic, Vesna Erakovic Haber, Radan Spaventi
Summary: Novel fast-acting azalides showed high activity against drug-resistant Plasmodium falciparum in vitro and demonstrated excellent antimalarial activity in a murine model. Pharmacokinetic analysis revealed stable metabolism, making these compounds ideal candidates for antimalarial therapy.
BRITISH JOURNAL OF PHARMACOLOGY
(2021)
Article
Chemistry, Inorganic & Nuclear
Elias Tanuhadi, Nadiia Gumerova, Alexander Prado-Roller, Markus Galanski, Hana Cipcic-Paljetak, Donatella Verbanac, Annette Rompel
Summary: A new monosubstituted aluminum Keggin-type germanotungstate has been synthesized and studied for its antibacterial properties, showing enhanced activity against Gram-negative and Gram-positive bacteria compared to inactive aluminum salt and indicating the redox activity as a dominating factor for the observed antibacterial activity.
INORGANIC CHEMISTRY
(2021)
Review
Medicine, General & Internal
Tomislav Mestrovic, Mario Matijasic, Mihaela Peric, Hana Cipcic Paljetak, Anja Baresic, Donatella Verbanac
Summary: The current research focuses on the relationship between gut microbiota and the development of bacteriuria and UTI, as well as the potential impact of vaginal dysbiosis and urinary microbiome perturbations on infections. Further research and exploration are needed to understand and potentially utilize these findings for the diagnosis and treatment of UTIs.
Review
Biochemistry & Molecular Biology
Donatella Verbanac, Andrea Ceri, Iva Hlapcic, Mehdi Shakibaei, Aranka Brockmueller, Bozo Kruslin, Neven Ljubicic, Neven Barsic, Dijana Detel, Lara Baticic, Lada Rumora, Anita Somborac-Bacura, Mario Stefanovic, Ivana Celap, Alma Demirovic, Roberta Petlevski, Jozsef Petrik, Marija Grdic Rajkovic, Andrea Hulina-Tomaskovic, Ivana Rako, Luciano Saso, Karmela Barisic
Summary: Drug-specific therapeutic approaches for colorectal cancer have significantly improved patient health, but there is a need for personalized treatments based on genetic and epigenetic tumor profiles. Circulating tumor DNA analysis has proven inaccurate, but detecting circulating tumor cells and protein analysis of exosomes show promise. Blood liquid biopsy has potential for the future, if results align with tissue analyses.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Review
Nutrition & Dietetics
Malgorzata Szczuko, Justyna Kikut, Urszula Szczuko, Iwona Szydlowska, Jolanta Nawrocka-Rutkowska, Maciej Zietek, Donatella Verbanac, Luciano Saso
Summary: This extensive narrative review discusses the modifications to the lifestyles of women with polycystic ovary syndrome, including metabolic pathways leading to disturbances and possible severe consequences such as diabetes and cancer. It proposes changes in diet patterns, selection of nutrients, supplementation with herbs, and physical activity as potential interventions to mitigate the impact of PCOS.
Article
Biochemistry & Molecular Biology
Goran Kragol, Victoria A. Steadman, Zorica Marusic Istuk, Ana Cikos, Martina Bosnar, Dubravko Jelic, Gabrijela Ergovic, Marija Trzun, Berislav Bosnjak, Ana Bokulic, Jasna Padovan, Ines Glojnaric, Vesna Erakovic Haber
Summary: This study reveals a novel azithromycin analog with good anti-inflammatory properties obtained via Barton-McCombie oxidation, which has the potential to treat chronic inflammatory diseases.
Article
Biochemistry & Molecular Biology
Petra Roskaric, Marcela Speranda, Tomislav Masek, Donatella Verbanac, Kristina Starcevic
Summary: The study shows that fructose intake leads to increased blood lipids, insulin resistance, and oxidative stress, as well as elevated monounsaturated fatty acid levels in adipose tissue. However, supplementation with DHA can reduce oxidative stress and improve adiponectin levels.
Article
Food Science & Technology
Kalle Norgren, Astrud Tuck, Antero Vieira Silva, Paula Burkhardt, Mattias Oberg, Vesna Munic Kos
Summary: This study introduces an in vitro adipogenesis model using high content analysis readouts to quantitatively compare adipocyte features on human MSCs. The research reveals that mixtures of bisphenols at sub-active concentrations induce a significant adipogenic effect due to their additive nature.
FOOD AND CHEMICAL TOXICOLOGY
(2022)
Article
Nutrition & Dietetics
Katarina Fehir Sola, Sanda Vladimir-Knezevic, Pero Hrabac, Iva Mucalo, Luciano Saso, Donatella Verbanac
Summary: This study found that multistrain probiotic supplementation is effective, safe, and well-tolerated in elderly patients with functional constipation. The supplementation led to a slight increase in stool frequency, with a prolonged effect observed in the probiotic group.
EUROPEAN JOURNAL OF CLINICAL NUTRITION
(2022)
Article
Gastroenterology & Hepatology
Hana Cipcic Paljetak, Anja Baresic, Marina Panek, Mihaela Peric, Mario Matijasic, Ivana Lojkic, Ana Barisic, Darija Vranesic Bender, Dina Ljubas Kelecic, Marko Brinar, Mirjana Kalauz, Marija Milicevic, Dora Grgic, Niksa Turk, Irena Karas, Silvija Cukovic-Cavka, Zeljko Krznaric, Donatella Verbanac
Summary: This study compares the differences in gut microbiota between newly diagnosed, treatment-naive adult inflammatory bowel disease (IBD) patients (CD and UC) with irritable bowel syndrome (IBS) patients and healthy individuals. The results show that changes in gut microbiota are detectable at the onset of disease and may play a significant role in the development of IBD.
Article
Chemistry, Medicinal
Mario-Livio Jelicic, Daniela Amidzic Klaric, Jelena Kovacic, Donatella Verbanac, Ana Mornar
Summary: This study compared various methods for determining the pharmacokinetic properties of biologically active compounds, and found that IAM-HPLC assay is the most suitable method for determining lipophilicity. It also revealed that HSA is the main protein binding these compounds in human plasma.
Review
Biochemistry & Molecular Biology
Jozsef Petrik, Donatella Verbanac, Marija Fabijanec, Andrea Hulina-Tomaskovic, Andrea Ceri, Anita Somborac-Bacura, Roberta Petlevski, Marija Grdic Rajkovic, Lada Rumora, Bozo Kruslin, Mario Stefanovic, Neven Ljubicic, Neven Barsic, Antonija Hanzek, Luka Bockor, Ivana Celap, Alma Demirovic, Karmela Barisic
Summary: This review provides an overview of the research conducted on circulating tumor cells (CTCs) with the aim of finding suitable detection systems and assessing the possibility of clinical applications in patients with colorectal cancer.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Chemistry, Inorganic & Nuclear
Morteza Rafieeshad, Nadiia I. Gumerova, Elias Tanuhadi, Gerald Giester, Hana Cipcic-Paljetak, Donatella Verbanac, Annette Rompel
Summary: Four new Ni-substituted Krebs-type sandwich-tungstobismuthates decorated with amino acids have been synthesized by one-pot solution methods. Only one compound showed antibacterial activity against four bacterial strains with minimum inhibitory concentration ranging from 8 to 256 μg/mL.
INORGANIC CHEMISTRY
(2023)
Article
Food Science & Technology
Danijela Pfeifer, Josip Resetar, Martin Stekovic, Magdalena Czlapka-Matyasik, Donatella Verbanac, Jasenka Gajdos Kljusuric
Summary: The aim of this study was to evaluate the diet quality and its relationship with lifestyle, body mass index (BMI), and field of study during COVID-19 lockdown periods in 2020 in Croatia. A total of 1939 students completed an online questionnaire assessing diet quality, which showed an improvement during both lockdown periods but to a lesser extent in the winter lockdown. Cooking for oneself was associated with better diet quality, while ordering or buying ready-to-eat food was linked to poorer diet quality. Decreased screen time and increased physical activity were associated with higher diet quality. Students with a BMI above 30 kg/m(2) had the lowest diet quality values. The study highlights the need for further research on the long-term impact of these changes post-COVID.
Article
Health Care Sciences & Services
Denis Horgan, Tanja Cufer, Francesco Gatto, Iwona Lugowska, Donatella Verbanac, Angela Carvalho, Jonathan A. Lal, Marta Kozaric, Sinead Toomey, Hristo Y. Ivanov, John Longshore, Umberto Malapelle, Samantha Hasenleithner, Paul Hofman, Catherine Alix-Panabieres
Summary: Liquid biopsy is a minimally invasive method aimed at detecting circulating tumor-derived components in body fluids, providing an alternative to tissue biopsies for cancer diagnosis confirmation. This paper explores the support provided by regulatory, policy, and governance frameworks for implementing LB technology in healthcare systems.
