Article
Pharmacology & Pharmacy
Wan-Jie Du, Huan Yang, Fang Tong, Shuai Liu, Chen Zhang, Yeying Chen, Yuze Yan, Yan-Wei Xiang, Ling-Yang Hua, Ye Gong, Zhi-Xiang Xu, Xiaoyan Liu, Xingyu Jiang, Mingfang Lu, Ji-Song Guan, Qingjian Han
Summary: This study reveals that primary sensory neurons modulate the cutaneous immune system by limiting the neuronal activity-dependent release of miR-let-7b, which can directly activate dermal dendritic cells via TLR7 and ultimately aggravate psoriatic lesions.
BRITISH JOURNAL OF PHARMACOLOGY
(2023)
Article
Cardiac & Cardiovascular Systems
Zhiyong Lei, Christine Wahlquist, Hamid el Azzouzi, Janine C. Deddens, Diederik Kuster, Alain van Mil, Agustin Rojas-Munoz, Manon M. Huibers, Mark Mercola, Roel de Weger, Jolanda van der Velden, Junjie Xiao, Pieter A. Doevendans, Joost P. G. Sluijter
Summary: miR-132/212 regulates cardiomyocyte contractility by targeting SERCA2a, with upregulation leading to impaired cardiac function. Pharmaceutical inhibition of miR-132/212 may be a promising therapeutic approach for heart failure patients.
FRONTIERS IN CARDIOVASCULAR MEDICINE
(2021)
Article
Cell Biology
Yat-Yin Law, Wei-Fang Lee, Chin-Jung Hsu, Yen-You Lin, Chun-Hao Tsai, Chien-Chung Huang, Min-Huan Wu, Chih-Hsin Tang, Ju-Fang Liu
Summary: The research on OA and RA revealed that miR-let-7c-5p and miR-149-5p have inhibitory effects on the transcription of proinflammatory cytokines, suggesting that increasing the expression of these two miRNAs is a novel therapeutic strategy for OA and RA.
Article
Cell Biology
Chunyu Bai, Qiwei Ren, Haifeng Liu, Xiangchen Li, Weijun Guan, Yuhua Gao
Summary: Pancreatic beta cell transplantation is the ideal method for treating type 1 diabetes, and producing beta cells from induced pluripotent stem cells (iPSCs) shows promise. This study demonstrates a novel approach for beta cell production and advocates for using iPSCs in cell replacement therapy for T1DM.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2021)
Article
Cell Biology
Andras Tomesz, Laszlo Szabo, Richard Molnar, Arpad Deutsch, Richard Darago, Bence L. Raposa, Nowrasteh Ghodratollah, Timea Varjas, Balazs Nemeth, Zsuzsanna Orsos, Eva Pozsgai, Jozsef L. Szentpeteri, Ferenc Budan, Istvan Kiss
Summary: Specific gene and miRNA expression patterns can serve as potential early biomarkers for harmful environmental carcinogen exposures. This research aimed to develop an assay panel using several miRNAs for the rapid screening of potential carcinogens. The study examined the expression changes of miR-124-1, miR-212, miR-132, miR-134, and miR-155 in the spleen, liver, and kidneys of mice following DMBA treatment. The results showed significant changes in the expression of miR-134, miR-132, and miR-124-1, suggesting their suitability as biomarkers for screening potential chemical carcinogens and monitoring the protective effects of chemopreventive agents.
Article
Oncology
Christopher A. Lucchesi, Jin Zhang, Buyong Ma, Ruth Nussinov, Xinbin Chen
Summary: The study reveals that RBM38 exerts opposite effects on Survivin expression through regulating two miRNAs, and an eight-amino acid peptide disrupts the RBM38-AGO2 complex to sensitize tumor spheroids to the Survivin inhibitor YM155.
Article
Multidisciplinary Sciences
Yusheng Huang, Limei Zhong, Kechao Nie, Lijuan Li, Shaohua Song, Fengbin Liu, Peiwu Li, Donglin Cao, Yufeng Liu
Summary: This study identified a novel competing endogenous RNA (ceRNA) network associated with the prognosis of lung adenocarcinoma (LUAD), suggesting potential key biomarkers or therapeutic targets for LUAD prognosis.
SCIENTIFIC REPORTS
(2021)
Article
Environmental Sciences
Valeria Longo, Gaspare Drago, Alessandra Longo, Silvia Ruggieri, Mario Sprovieri, Fabio Cibella, Paolo Colombo
Summary: There is growing evidence that environmental pollutants can induce epigenetic modifications altering miRNA expression and leading to pathological conditions in animals. This study found associations between serum concentrations of various inorganic and organic pollutants and the expression of miRNAs in pregnant women. These findings provide innovative early-warning approaches to human health risk assessment.
SCIENCE OF THE TOTAL ENVIRONMENT
(2022)
Article
Multidisciplinary Sciences
G. P. Vadla, B. Daghat, N. Patterson, V Ahmad, G. Perez, A. Garcia, Y. Manjunath, J. T. Kaifi, G. Li, C. Y. Chabu
Summary: Low-dose computed tomography (LDCT) Non-Small Cell Lung (NSCLC) screening has a high false-positive rate, and there is a need for minimally invasive approaches to improve the accuracy of NSCLC diagnosis. Additionally, NSCLC patients treated with Osimertinib often develop resistance, and the underlying mechanisms are not fully understood. This study found that a combination of plasma extracellular vesicle (EV) and circulating microRNA (miRNA) can effectively discriminate between NSCLC patients and high-risk cancer-free controls, and certain miRNAs play a role in cancer therapy resistance signals.
SCIENTIFIC REPORTS
(2022)
Article
Physiology
Jian Han, Xiaogang Cui, Tianqi Yuan, Zhiming Yang, Yue Liu, Yajuan Ren, Changxin Wu, Yunfei Bian
Summary: Circulating exosomal miRNAs have potential as biomarkers for diagnosis of cardiovascular diseases. In this study, the differentially expressed miRNAs in exosomes from plasma of SCAD patients were analyzed and their diagnostic potential for SCAD was investigated.
FRONTIERS IN PHYSIOLOGY
(2023)
Article
Cardiac & Cardiovascular Systems
Raquel Costa Silva Dantas-Komatsu, Marina Sampaio Cruz, Paula Paccielli Freire, Rosiane Viana Zuza Diniz, Raul Hernandes Bortolin, Otavio Cabral-Marques, Kamilla Batista da Silva Souza, Mario Hiroyuki Hirata, Rosario Dominguez Crespo Hirata, Bruna Zavarize Reis, Igor Jurisica, Vivian Nogueira Silbiger, Andre Ducati Luchessi
Summary: This study examined the involvement of microRNAs in peripheral blood mononuclear cells of post-myocardial infarction patients with left ventricular systolic dysfunction (LVSd). The upregulation of let-7b-5p, miR-326, and miR-125a-3p in LVSd patients suggests their potential as biomarkers for cardiac dysfunction.
FRONTIERS IN CARDIOVASCULAR MEDICINE
(2023)
Article
Biochemistry & Molecular Biology
Paulo Ricardo da Silva Gomes, Patricia Candido, Vitoria Ghazarian, Juliana A. Camargo, Vanessa R. Guimaraes, Guilherme L. Goncalves, Poliana Romao, Iran A. Silva, Miguel Srougi, William C. Nahas, Katia R. Leite, Sabrina T. Reis, Ruan Pimenta, Nayara Izabel Viana
Summary: Bladder cancer, a leading transitional cell carcinoma, has high morbidity and mortality rates, motivating the development of new molecular target therapies utilizing microRNAs. This study demonstrated that transfection with miR-Let-7c precursor mimic resulted in increased apoptosis, decreased migration, invasion potential, and cell proliferation in T24 cells. It suggests that miR-Let-7c may play a role in inhibiting cell proliferation and promoting apoptosis in muscle-invasive urothelial carcinoma cells.
MOLECULAR BIOLOGY REPORTS
(2021)
Article
Medicine, Research & Experimental
Keli You, Yuejun Liu, Le Chen, Haiyan Ye, Wumei Lin
Summary: miR-let-7b plays a key regulatory role in ovarian cancer chemoresistance, and combining RRTS with Taxol enhances the therapeutic effect on ovarian cancer cells.
EXPERIMENTAL AND THERAPEUTIC MEDICINE
(2022)
Article
Dentistry, Oral Surgery & Medicine
Weilian Liang, Yang Chen, Hanzhe Liu, Hui Zhao, Tingting Luo, Hokeung Tang, Xiaocheng Zhou, Erhui Jiang, Zhe Shao, Ke Liu, Zhengjun Shang
Summary: Oral squamous cell carcinoma-derived small extracellular vesicles promote the precancerous transformation of normal epithelial cells through the miR-let-7c/p53/PTEN pathway.
INTERNATIONAL JOURNAL OF ORAL SCIENCE
(2022)
Article
Endocrinology & Metabolism
Yun Liu, Chao-Yue Su, Yan-Yan Yan, Jian Wang, Jia-Jun Li, Ji-Jun Fu, Yu-Qing Wang, Jian-Ye Zhang
Summary: This research uncovers that exosomal let-7c-5p and miR-181b-5p derived from A549 cells can induce migration, invasion, and EMT in BEAS-2B cells, potentially through the regulation of focal adhesion and MAPK signaling pathway.
FRONTIERS IN ENDOCRINOLOGY
(2022)
Article
Pharmacology & Pharmacy
Sabrina Yamoune, Katharina Wintz, Christian Niederau, Rogerio B. Craveiro, Michael Wolf, Julia Stingl
Summary: The study found that genetic polymorphism in the CYP2C8 gene may be associated with disturbed periodontal remodeling in periodontal fibroblasts, potentially contributing to the risk of side effects of antiresorptive bone treatment.
BASIC & CLINICAL PHARMACOLOGY & TOXICOLOGY
(2022)
Article
Pharmacology & Pharmacy
Nersi Alaeddin, Julia C. Stingl, Monique M. B. Breteler, Folgerdiena M. de Vries
Summary: The study aimed to evaluate the reliability of self-reported medication use by comparing it with drug-specific metabolites measured in plasma in a population-based cohort in Germany. The results showed high concordance rates between self-reported drug use and metabolites, suggesting that self-reported drug use is reliable and accurate for assessing drug use.
BRITISH JOURNAL OF CLINICAL PHARMACOLOGY
(2022)
Article
Geriatrics & Gerontology
Matthias Klimek, Raphael Simon Peter, Michael Denkinger, Dhayana Dallmeier, Kilian Rapp, Dietrich Rothenbacher, Jochen Klenk
Summary: This study investigated the associations between weather conditions and walking duration and time spent out-of-home (TOH) among older adults. The results showed that higher temperatures, solar radiation, and sunshine duration led to increased walking duration and TOH, while higher precipitation, humidities, and windspeeds led to a decrease. Women generally had longer walking duration and TOH times than men.
EUROPEAN REVIEW OF AGING AND PHYSICAL ACTIVITY
(2022)
Article
Oncology
Konstantin Weber-Lassalle, Corinna Ernst, Alexander Reuss, Kathrin Mollenhoff, Klaus Baumann, Christian Jackisch, Jan Hauke, Dimo Dietrich, Julika Borde, Tjoung-Won Park-Simon, Lars Hanker, Katharina Prieske, Sandra Schmidt, Nana Weber-Lassalle, Esther Pohl-Rescigno, Stefan Kommoss, Frederik Marme, Florian Heitz, Julia C. Stingl, Rita K. Schmutzler, Philipp Harter, Eric Hahnen
Summary: Cancer patients, particularly those with ovarian cancer, have a high prevalence of blood-specific mutations in clonal hematopoiesis (CH)-associated genes, which may be influenced by genetic factors.
JNCI-JOURNAL OF THE NATIONAL CANCER INSTITUTE
(2022)
Article
Biotechnology & Applied Microbiology
Bianza T. Mbavha, Comfort R. Kanji, Nadina Stadler, Julia Stingl, Andrea Stanglmair, Catharina Scholl, William Wekwete, Collen Masimirembwa
Summary: This study sequenced clinically actionable genetic variants in a Zimbabwean population using a custom-designed array, and identified metabolism-related genes for commonly used drugs in Zimbabwe. The results showed significant genetic variations in the Zimbabwean population, which are important for the safe and efficacious use of drugs. The established cohort of genotyped volunteers can be used for future pharmacogenetic studies on drugs for people of African ancestry.
PHARMACOGENETICS AND GENOMICS
(2022)
Article
Genetics & Heredity
Jochen Fracowiak, Tatjana Huebner, Steffen Hess, Christoph Roethlein, Daria Langner, Udo Schneider, Felix Falkenberg, Catharina Scholl, Roland Linder, Julia Stingl, Britta Haenisch, Michael Steffens
Summary: This study, EMPAR, elucidates the impact of genetic variability of pharmacogenes as a possible risk factor for adverse drug reactions. The study evaluates the possible associations of pharmacogenetically predicted metabolic profiles relevant for the metabolism of frequently prescribed cardiovascular drugs and performs analyses and evaluations on a German study population.
PHARMACOGENOMICS JOURNAL
(2022)
Review
Pharmacology & Pharmacy
Seenae Eum, Franklin Sayre, Adam M. Lee, Julia C. Stingl, Jeffrey R. Bishop
Summary: This study aimed to quantify the association of CYP2B6 variant alleles and genotype-defined metabolizer phenotypes with bupropion and HB exposure. The results showed that the CYP2B6*6 allele and genotype-defined CYP2B6 poor and intermediate metabolizer phenotypes are associated with significantly lower exposures to HB and the total active moiety.
Article
Virology
Marie-Luise Herrlein, Sascha Hein, Tobias Zahn, Ines Mhedhbi, Jan Raupach, Younes Husria, Nuka Ivalu Benz, Jonathan Eisert, Daniela Bender, Vanessa Haberger, Florian D. Hastert, Lisa Henss, Barbara S. Schnierle, Julia C. Stingl, Michael Dreher, Eberhard Hildt
Summary: With the increasing number of vaccinated and convalescent individuals, there is a need for robust methods to quantify neutralizing antibodies. This study compared different neutralization assays using sera from hospitalized COVID-19 patients and found comparable data. The results provide insights into the neutralizing potential of SARS-CoV-2-specific sera.
Article
Pharmacology & Pharmacy
Ingmar Bergs, Katja S. Just, Catharina Scholl, Michael Dreher, Julia C. Stingl
Summary: This study aimed to analyze the association between inhaled therapy and adverse drug reactions (ADRs) in COPD patients, with a focus on the impact of overdose. The results showed that overdose of inhaled therapies led to severe ADRs and affected emergency room presentations. These ADRs are rarely associated with inhaled therapy by healthcare professionals or patients. Due to the high volume of prescriptions for inhaled drugs, greater attention should be paid to pharmacovigilance and patient education in COPD patients.
EUROPEAN JOURNAL OF CLINICAL PHARMACOLOGY
(2023)
Article
Biochemistry & Molecular Biology
Irena Voinsky, Oleg Y. Fridland, Adi Aran, Richard E. Frye, David Gurwitz
Summary: Early diagnosis of ASD is crucial, but the current process is lengthy due to the lack of reliable biomarkers. In this study, we used RNA-sequencing to identify dysregulated genes in ASD children and developed machine learning models for ASD diagnosis. Our models achieved an accuracy of 82% in distinguishing ASD children from NT children, but further validation is needed.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Psychiatry
Noa Katz Shroitman, Assif Yitzhaky, Dorit Ben Shachar, David Gurwitz, Libi Hertzberg
Summary: Schizophrenia is a chronic and debilitating mental disorder with unknown causes. This study investigated the expression of ATP synthase encoding genes in postmortem brain samples of individuals with schizophrenia. The results showed down-regulation of ATP synthase genes in schizophrenia, supporting the hypothesis that reduced ATP synthesis is associated with this disorder. The findings highlight the importance of abnormal cellular energy metabolism in schizophrenia.
JOURNAL OF PSYCHIATRIC RESEARCH
(2023)
Article
Pediatrics
Sarah Leitzen, Diana Dubrall, Irmgard Toni, Julia Stingl, Patrick Christ, Ursula Koberle, Matthias Schmid, Antje Neubert, Bernhardt Sachs
Summary: The study compares the differences between ADRs reported in the KiDSafe project and the spontaneous ADR reports sent to EudraVigilance. The results show underreporting of ADRs in the spontaneous reports compared to the systematically collected KiDSafe reports. The two approaches yield different results in terms of reported drugs, ADRs, and medication errors.
Article
Chemistry, Medicinal
Irena Voinsky, Elad Shapira, David Gurwitz
Summary: Biobanks play a crucial role in biomedical research by providing human cell lines for drug development projects. RNA-sequencing of large panels of cell lines from individuals with certain disorders and healthy controls, or with different drug response phenotypes, is commonly performed. In this study, we demonstrate that extracting RNAs directly from long-term frozen human cell lines yields high-quality RNA samples suitable for optimal RNA-sequencing, comparable to those obtained from growing cell lines.
DRUG DEVELOPMENT RESEARCH
(2023)
Editorial Material
Chemistry, Medicinal
David Gurwitz, Noam Shomron
DRUG DEVELOPMENT RESEARCH
(2023)
Article
Biochemistry & Molecular Biology
Liron Mizrahi, Ashwani Choudhary, Polina Ofer, Gabriela Goldberg, Elena Milanesi, John R. Kelsoe, David Gurwitz, Martin Alda, Fred H. Gage, Shani Stern
Summary: Bipolar disorder is a mood disorder characterized by recurrent episodes of mania and depression. Many patients do not respond to the first-line treatment drug lithium, making clinical management challenging. By analyzing gene expression in lymphoblastoid cell lines, we identified potential biomarkers for diagnosing and treating bipolar disorder. We also developed machine learning algorithms that accurately predicted lithium response in different patient cohorts. This methodology shows promise for guiding therapeutic interventions.
MOLECULAR PSYCHIATRY
(2023)
