Article
Biochemical Research Methods
Nadja Mallock, Andrea Rabenstein, Peter Laux, Tobias Ruether, Christoph Hutzler, Maria Kristina Parr, Andreas Luch
Summary: A quantitative method based on LC-ESI-MS/MS was developed and validated for reliable determination of nicotine and its metabolites in blood plasma. The method showed good accuracy and precision for quantitation of nicotine, cotinine, and hydroxycotinine in plasma samples. Optimization of sample preparation and chromatographic conditions were crucial for successful application of the method.
JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES
(2021)
Article
Pharmacology & Pharmacy
Paulo Roberto Xavier Tomaz, Thuane Sales Goncalves, Juliana Rocha Santos, Jaqueline Scholz, Tania Ogawa Abe, Patricia Viviane Gaya, Eduardo Costa Figueiredo, Henrique Dipe de Faria, Isarita Martins, Ana Miguel Fonseca Pego, Beatriz Aparecida Bismara, Mauricio Yonamine, Alexandre Costa Pereira, Paulo Caleb Lima Santos Junior
Summary: This study aimed to evaluate the application of nicotine metabolite ratio (NMR) in Brazilian smoking patients and analyze its association with smoking characteristics. The findings contribute to the development of personalized smoking cessation treatments and improvement of success rates.
FRONTIERS IN PHARMACOLOGY
(2022)
Article
Biochemistry & Molecular Biology
Jacek Baj, Wojciech Flieger, Dominika Przygodzka, Grzegorz Buszewicz, Grzegorz Teresinski, Magdalena Pizon, Ryszard Maciejewski, Jolanta Flieger
Summary: This study developed and compared two new chromatographic methods, HPLC-QQQ-MS/MS and RP-HPLC-DAD enriched with chaotropic additives, for the determination of nicotine and its major metabolites in human plasma. The results showed that HPLC-QQQ-MS/MS had lower limits of detection, while RP-HPLC-DAD enriched with chaotropic additives had higher extraction efficiency.
Article
Biochemistry & Molecular Biology
Hyun-Seung Lee, Mi-Ryung Chun, Soo-Youn Lee
Summary: Measuring multiple nicotine metabolites and total nicotine equivalents (TNE) is a more reliable method for verifying tobacco exposure compared to measuring single urinary cotinine alone. This measurement can be helpful for monitoring low-level or secondhand smoke exposure and for determining individual differences in nicotine metabolism.
Article
Environmental Sciences
Mohd Rehan, Ummer R. Zargar, Ishfaq A. Sheikh, Saif A. Alharthy, Majed N. Almashjary, Adel M. Abuzenadah, Mohd A. Beg
Summary: Tobacco/nicotine poses a significant threat to global public health and has harmful effects on various systems of the human body. This study found that nicotine and its metabolites can interfere with the binding of carrier proteins and hormones, leading to an imbalance in hormone transport and homeostasis in the blood circulation.
Article
Public, Environmental & Occupational Health
Kaitlyn O. Browning, Michael J. DeSarno, Danielle R. Davis, Joanna M. Streck, Cecilia L. Bergeria, Roxanne F. Harfmann, Maria A. Parker, Sarah H. Heil, Stacey C. Sigmon, Diann E. Gaalema, Jennifer W. Tidey, Dustin C. Lee, Haley J. Tetreault, Stephen T. Higgins
Summary: This study uses multivariate regression to investigate the contribution of the reinforcing value of smoking and nicotine-dependence severity to individual differences in nicotine exposure levels among vulnerable populations. The results suggest that differences in reinforcing value may underpin a considerable portion of the variance in nicotine exposure accounted for by dependence severity.
PREVENTIVE MEDICINE
(2022)
Article
Engineering, Biomedical
Jaroslav A. Hubacek, Ivana Kurcova, Vera Maresova, Alexandra Pankova, Lenka Stepankova, Kamila Zvolska, Vera Lanska, Eva Kralikova
Summary: The study analyzed the impact of smoking-related polymorphisms on plasma values of nicotine metabolites, finding significant associations between certain genetic variants and specific metabolite ratios. However, these associations were not directly linked to nicotine abstinence rates.
BIOMEDICAL PAPERS-OLOMOUC
(2021)
Article
Biochemistry & Molecular Biology
E. Melinda Mahabee-Gittens, Georg E. Matt, Matthew J. Mazzella, John T. Doucette, Parita Ratnani, Ashley L. Merianos
Summary: This study examined the associations between inflammatory markers and pediatric illnesses related to children's exposure to tobacco smoke. The results showed that parent-reported child tobacco smoke exposure was associated with increased levels of C-reactive protein (CRP) in children, while children with bacterial diagnoses had higher levels of IL-8.
Article
Medicine, Research & Experimental
Krista R. Schaefer, Jaedon P. Avey, Michael R. Todd, Julie A. Beans, Denise A. Dillard, Laura M. Shireman, Timothy A. Thornton, Rachel F. Tyndale, Kenneth E. Thummel, Renee F. Robinson, Katrina G. Claw
Summary: Smoking prevalence is higher in Alaska Native and American Indian populations, due in part to genetic factors such as variations in the CYP2A6 gene. This study found that genetic variation in CYP2A6 contributes to differences in nicotine metabolism, impacting smoking cessation efforts in this population.
CTS-CLINICAL AND TRANSLATIONAL SCIENCE
(2021)
Article
Oncology
Shannon S. Cigan, Sharon E. Murphy, Daniel O. Stram, Stephen S. Hecht, Loic Le Marchand, Irina Stepanov, Sungshim L. Park
Summary: A study found that urinary total 3-HCOT/cotinine, 3-HPMA, and cadmium are positively associated with lung cancer risk among current smokers. These biomarkers may provide additional information on lung cancer risk.
CANCER EPIDEMIOLOGY BIOMARKERS & PREVENTION
(2023)
Article
Substance Abuse
Haidy K. Giratallah, Meghan J. Chenoweth, Newton Addo, Jasjit S. Ahluwalia, Lisa Sanderson Cox, Caryn Lerman, Tony P. George, Neal L. Benowitz, Rachel F. Tyndale
Summary: This study found that urinary NMRT/F showed the highest correlation with plasma NMR, making it the best urinary alternative to plasma NMR or nicotine clearance. NMRT/T had the least utility due to its substantial influence from cotinine glucuronidation.
DRUG AND ALCOHOL DEPENDENCE
(2021)
Article
Pharmacology & Pharmacy
Ahmed El-Boraie, Meghan J. Chenoweth, Jennie G. Pouget, Neal L. Benowitz, Koya Fukunaga, Taisei Mushiroda, Michiaki Kubo, Nicole L. Nollen, Lisa Sanderson Cox, Caryn Lerman, Jo Knight, Rachel F. Tyndale
Summary: The Nicotine Metabolite Ratio (NMR) is influenced by smoking behavior, diseases, and cessation outcomes. Genetic risk scores based on CYP2A6 for European and African populations can accurately reflect metabolizer types and predict the NMR.
CLINICAL PHARMACOLOGY & THERAPEUTICS
(2021)
Article
Substance Abuse
Connie S. Sosnoff, Kevin Caron, J. Ricky Akins, Kristin Dortch, Ronald E. Hunter, Brittany N. Pine, June Feng, Benjamin C. Blount, Yao Li, Dana M. van Bemmel, Heather L. Kimmel, Kathryn C. Edwards, Maciej L. Goniewicz, Dorothy K. Hatsukami, B. Rey deCastro, John T. Bernert, Stephen Arnstein, Nicolette Borek, Ying Deng-Bryant, Elena Mishina, Charles Lawrence, Andrew Hyland, Stephen S. Hecht, Kevin P. Conway, James L. Pirkle, Lanqing Wang
Summary: This study provides nationally representative estimates of nicotine biomarker concentrations in tobacco users and nonusers, which can serve as reference ranges for characterizing nicotine exposure in the US population.
NICOTINE & TOBACCO RESEARCH
(2022)
Article
Genetics & Heredity
Alec W. R. Langlois, Ahmed El-Boraie, Jennie G. G. Pouget, Lisa Sanderson Cox, Jasjit S. S. Ahluwalia, Koya Fukunaga, Taisei Mushiroda, Jo Knight, Meghan J. J. Chenoweth, Rachel F. F. Tyndale
Summary: CYP2A6 gene exhibits polymorphisms, including gene deletions, duplications, and hybrids with the CYP2A7 pseudogene. This study developed a reliable protocol for genotyping CYP2A6 structural variants, investigated their association with lung cancer risk, and explored the feasibility of CYP2A6 structural variant imputation from SNP array data.
JOURNAL OF HUMAN GENETICS
(2023)
Article
Biochemistry & Molecular Biology
Jadwiga Buchwald, Meghan J. Chenoweth, Teemu Palviainen, Gu Zhu, Christian Benner, Scott Gordon, Tellervo Korhonen, Samuli Ripatti, Pamela A. F. Madden, Terho Lehtimaki, Olli T. Raitakari, Veikko Salomaa, Richard J. Rose, Tony P. George, Caryn Lerman, Matti Pirinen, Nicholas G. Martin, Jaakko Kaprio, Anu Loukola, Rachel F. Tyndale
Summary: This study conducted the largest GWAS to date on NMR in European ancestry current smokers, identifying multiple genetic variants associated with nicotine clearance rate. Understanding the genetic factors influencing smoking-related traits can facilitate epidemiological studies of smoking and disease, and optimize smoking cessation support to reduce the personal and societal costs associated with smoking.
MOLECULAR PSYCHIATRY
(2021)
Article
Pharmacology & Pharmacy
Eliza R. McColl, Jacinda Kwok, Neal L. Benowitz, Christi A. Patten, Christine A. Hughes, Kathryn R. Koller, Christie A. Flanagan, Timothy K. Thomas, Vanessa Y. Hiratsuka, Rachel F. Tyndale, Micheline Piquette-Miller
Summary: Prenatal tobacco use affects the expression of multiple placental transporters, and this effect differs by fetal sex. Transcript levels of multiple transporters significantly correlate with tobacco biomarkers in maternal and fetal urine. Eliminating prenatal tobacco use should alleviate these changes.
CLINICAL PHARMACOLOGY & THERAPEUTICS
(2023)
Article
Genetics & Heredity
Alec W. R. Langlois, Ahmed El-Boraie, Jennie G. G. Pouget, Lisa Sanderson Cox, Jasjit S. S. Ahluwalia, Koya Fukunaga, Taisei Mushiroda, Jo Knight, Meghan J. J. Chenoweth, Rachel F. F. Tyndale
Summary: CYP2A6 gene exhibits polymorphisms, including gene deletions, duplications, and hybrids with the CYP2A7 pseudogene. This study developed a reliable protocol for genotyping CYP2A6 structural variants, investigated their association with lung cancer risk, and explored the feasibility of CYP2A6 structural variant imputation from SNP array data.
JOURNAL OF HUMAN GENETICS
(2023)
Article
Neurosciences
Dara G. Ghahremani, Jean-Baptiste F. Pochon, Maylen Perez Diaz, Rachel F. Tyndale, Andy C. Dean, Edythe D. London
Summary: Nicotine dependence is a major predictor of relapse in individuals with Tobacco Use Disorder. The study examines the relationship between nicotine dependence and the sub-regions of the insular cortex, suggesting potential therapeutic approaches based on targeting specific insular subnetworks.
NEUROPSYCHOPHARMACOLOGY
(2023)
Article
Clinical Neurology
Christine Ibrahim, Victor M. Tang, Daniel M. Blumberger, Saima Malik, Rachel F. Tyndale, Alisson P. Trevizol, Mera S. Barr, Zafiris J. Daskalakis, Abraham Zangen, Bernard Le Foll
Summary: This study demonstrates the potential benefit of using insula repetitive transcranial magnetic stimulation (rTMS) with the H11 coil in combination with varenicline for smoking cessation. The combined treatment showed a higher rate of success in achieving abstinence at the end of the treatment period. However, there were no significant differences between the groups in terms of other smoking outcomes and adverse events. Maintenance rTMS sessions and continuation of varenicline for individuals who achieve abstinence may lead to long-term effects and should be considered in future studies.
Article
Neurosciences
Nadia Boachie, Erin Gaudette, Richard P. Bazinet, Lin Lin, Rachel F. Tyndale, Esmaeil Mansouri, Marilyn A. Huestis, Junchao Tong, Bernard Le Foll, Stephen J. Kish, Tony P. George, Isabelle Boileau
Summary: Our preliminary findings suggest that the activity of the endocannabinoid system is elevated in chronic cannabis users. Further studies are needed to examine endocannabinoids and NAEs during prolonged abstinence and the potential role of DHEA in craving.
Article
Substance Abuse
Jaedon P. Avey, Krista R. Schaefer, Carolyn J. Noonan, Susan B. Trinidad, Clemma J. Muller, Katrina G. Claw, Denise A. Dillard, Michael R. Todd, Julie A. Beans, Rachel F. Tyndale, Renee F. Robinson, Kenneth E. Thummel
Summary: This study aimed to evaluate the contributions of nicotine metabolite ratio, cessation medication, demographics, and tobacco use history to quit success. The findings revealed that the use of varenicline and bupropion increased the likelihood of quitting, compared to nicotine replacement therapy. Non-optimal medication and phenotype concordance decreased the chances of successful cessation.
NICOTINE & TOBACCO RESEARCH
(2023)
Article
Substance Abuse
Scott S. Lee, Yuchiao Chang, Nancy A. Rigotti, Daniel E. Singer, Douglas E. Levy, Rachel F. Tyndale, Esa M. Davis, Matthew S. Freiberg, Stephen King, Quinn S. Wells, Hilary A. Tindle
Summary: The nicotine metabolite ratio (NMR) is a biomarker that predicts the efficacy of nicotine replacement therapy (NRT), with fast metabolizers benefiting less than slow metabolizers. This study aimed to investigate whether treatment support can improve smoking cessation outcomes in fast metabolizers.
NICOTINE & TOBACCO RESEARCH
(2023)
Article
Substance Abuse
Meghan J. Chenoweth, Caryn Lerman, Jo Knight, Rachel F. Tyndale
Summary: The goal of this study was to determine how genetic variation in CYP2A6 and nicotine dependence impact smoking cessation, and whether dependence could refine treatment recommendations. The results indicated that nicotine dependence neither mediated nor moderated the association between CYP2A6 variation and smoking cessation. Incorporating information on both CYP2A6 and nicotine dependence severity may improve smoking cessation strategies.
NICOTINE & TOBACCO RESEARCH
(2023)
Article
Substance Abuse
Nicole M. Roeder, Abrianna Mihalkovic, Brittany J. Richardson, Samantha L. Penman, Maria Novalen, Nikki Hammond, Rina Eiden, Jibran Y. Khokhar, Rachel F. Tyndale, Panayotis K. Thanos
Summary: This study aimed to extend previous research on nicotine inhalation to female animals and provide behavioral and pharmacokinetic data. The results showed that while nicotine serum concentrations were similar between inhalation and injection, the route of administration had differential effects on locomotor behavior.
NICOTINE & TOBACCO RESEARCH
(2023)
Article
Biochemistry & Molecular Biology
Alec W. R. Langlois, Jennie G. Pouget, Jo Knight, Meghan J. Chenoweth, Rachel F. Tyndale
Summary: The deletion of CYP2A7 is associated with ovarian cancer risk, but CYP2A6 structural variants do not affect ovarian cancer risk and lower the risk of lung cancer.
EUROPEAN JOURNAL OF HUMAN GENETICS
(2023)
Article
Substance Abuse
Robert K. Cooper Jr, Martin C. Mahoney, Stephen T. Tiffany, Craig R. Colder, Rachel F. Tyndale, Larry W. Hawk Jr
Summary: People who metabolize nicotine more quickly are generally less successful at quitting smoking, but the mechanisms linking nicotine metabolism to smoking reinforcement and craving are unclear. This study found that in a sample of adults seeking to quit smoking, higher metabolism rate was not associated with greater smoking reinforcement or craving.
NICOTINE & TOBACCO RESEARCH
(2023)
Article
Pharmacology & Pharmacy
Intishar Kazi, Meghan J. Chenoweth, Didier Jutras-Aswad, Keith Ahamad, M. Eugenia Socias, Bernard Le Foll, Rachel F. Tyndale
Summary: Methadone and buprenorphine/naloxone are effective treatments for opioid use disorder, but individual responses can vary due to genetic factors. A study investigated the association between genetic variants and clinical outcomes in patients receiving these treatments for 24 weeks. The results showed that OPRM1 gene rs1799971 variant was associated with a better response to methadone treatment, with individuals carrying the AA genotype having more opioid-free urine screens.
CLINICAL PHARMACOLOGY & THERAPEUTICS
(2023)
Letter
Substance Abuse
John A. Cunningham, Scott T. Leatherdale, Michael Chaiton, Rachel F. Tyndale, Christina Schell, Alexandra Godinho
NICOTINE & TOBACCO RESEARCH
(2023)
Article
Substance Abuse
Dana Mowls Carroll, Sharon Murphy, Ellen Meier, Kristine Rhodes, Casey Dorr, Greg Braaten, Pamala A. Jacobson, Linda Frizzell, Rachel F. Tyndale, Dorothy Hatsukami, Carol Hernandez
Summary: This study aimed to investigate the feasibility of tailoring pharmacotherapy to the nicotine metabolite ratio (NMR) and its potential significance for American Indian individuals. The results showed a significant correlation between NMR and cigarette dependence, but the acceptability of NMR-informed pharmacotherapy was lower in this tribe.
NICOTINE & TOBACCO RESEARCH
(2023)
Article
Substance Abuse
Sarah S. Tonkin, Craig Colder, Martin C. Mahoney, Gary E. Swan, Paul Cinciripini, Robert Schnoll, Tony P. George, Rachel F. Tyndale, Larry W. Hawk
Summary: This study found that varenicline can improve smoking cessation rates by attenuating changes in negative affect and craving. Positive affect was not a significant mediator in this process. The research emphasizes the importance of reducing negative affect and early craving for effective interventions and suggests that negative affect, positive affect, and craving are all associated with relapse, making them potential treatment targets for future intervention development.
NICOTINE & TOBACCO RESEARCH
(2022)
