Article
Chemistry, Medicinal
Massimo Moro, Orazio Fortunato, Giulia Bertolini, Mavis Mensah, Cristina Borzi, Giovanni Centonze, Francesca Andriani, Daniela Di Paolo, Patrizia Perri, Mirco Ponzoni, Ugo Pastorino, Gabriella Sozzi, Mattia Boeri
Summary: Despite improvements in therapies and screening strategies, lung cancer prognosis remains poor, especially for patients with metastatic tumors. Cancer stem cells (CSCs) are a major cause of the low survival rate, and microRNA miR-486-5p has been identified as a potential therapeutic target for lung cancer.
Article
Multidisciplinary Sciences
Xiong Shu, Weifeng Liu, Huiqi Liu, Hui Qi, Chengai Wu, Yu-Liang Ran
Summary: This study identified differential expression of miRNAs between CD133(+) and CD133(-) cells, with miR-4284 being a particularly interesting miRNA that may have significant roles in the function of OSCs, especially associated with the Wnt signaling pathway.
Article
Biology
Kamal Shaik Fakiruddin, Moon Nian Lim, Norshariza Nordin, Rozita Rosli, Syahril Abdullah
Summary: This study demonstrated that pre-treatment with first-line chemotherapies could enhance the inhibitory effect of MSC-TRAIL on NSCLC-derived CSCs, particularly on TRAIL-resistant CSCs. The findings highlight the potential of combining chemotherapies and MSC-TRAIL as a novel approach for NSCLC treatment.
Article
Biochemistry & Molecular Biology
Bartlomiej Pawlik, Szymon Grabia, Urszula Smyczynska, Wojciech Fendler, Izabela Drozdz, Ewa Liszewska, Jacek Jaworski, Katarzyna Kotulska, Sergiusz Jozwiak, Wojciech Mlynarski, Joanna Trelinska
Summary: This study suggests that miRNA dysregulation plays a role in the pathogenesis of TSC, with certain miRNAs potentially serving as markers for treatment efficacy and targets for future therapy. Results also indicate common genes targeted by the dysregulated miRNAs, although no changes in mRNA expression of these targets were observed after rapamycin treatment.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Health Care Sciences & Services
Antonia Harangus, Raduly Lajos, Livia Budisan, Oana Zanoaga, Cristina Ciocan, Cecilia Bica, Radu Pirlog, Ioan Simon, Marioara Simon, Cornelia Braicu, Ioana Berindan-Neagoe
Summary: This study evaluates miRNA expression patterns and specific mechanisms in male patients with NSCLC. The results identify several overexpressed and underexpressed miRNAs, as well as a common miRNA signature. Additionally, mRNA-miRNA regulatory networks were created to identify critical molecules and reveal NSCLC signaling pathways. These findings contribute to understanding the mechanisms involved in NSCLC development and offer opportunities for potential therapeutic targets.
JOURNAL OF PERSONALIZED MEDICINE
(2022)
Article
Oncology
Juhong Wang, Fei Shao, Yannan Yang, Wei Wang, Xueying Yang, Renda Li, Hong Cheng, Sijin Sun, Xiaoli Feng, Yibo Gao, Jie He, Zhimin Lu
Summary: This study revealed a non-metabolic function of HK2 in promoting cancer cell stemness and provided new insights into the multifaceted roles of HK2 in tumor development.
CANCER COMMUNICATIONS
(2022)
Article
Biochemistry & Molecular Biology
Huan Ma, Siyu Jiang, Yinan Yuan, Ji Li, Yizhuo Li, Yanping Lv, Tengjiao Du, Jingqian Guan, Xizi Jiang, Lei Tian, Qianqian Zheng, Lianhe Yang, Qingchang Li
Summary: RUNX1, a member of the RUNX family, is involved in the regulation of non-small cell lung cancer. It promotes cell proliferation and migration via the mTOR pathway and may serve as a potential therapeutic target for NSCLC.
Article
Biochemistry & Molecular Biology
Yan S. Kim, Daria M. Potashnikova, Alisa M. Gisina, Irina Kholodenko, Arthur T. Kopylov, Olga Tikhonova, Leonid K. Kurbatov, Aleena A. Saidova, Anna Tvorogova, Roman Kholodenko, Pavel Belousov, Ivan A. Vorobjev, Victor G. Zgoda, Konstantin N. Yarygin, Alexey Yu Lupatov
Summary: CD133 plasma membrane expression is positively correlated with the proliferative activity of cancer cells. Transcriptomic and proteomic profiling revealed minor distinctions between different levels of CD133 expression. The TRIM28 transcription factor plays a prominent role in regulating CD133 expression. Knockout of the TRIM28 gene downregulates CD133 expression in Caco2 cell clones.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Biochemistry & Molecular Biology
Margarita Pustovalova, Taisia Blokhina, Lina Alhaddad, Anna Chigasova, Roman Chuprov-Netochin, Alexander Veviorskiy, Gleb Filkov, Andreyan N. Osipov, Sergey Leonov
Summary: Cancer stem cells play a critical role in non-small cell lung cancer, and differences in DNA-damage signaling and dormant features have been observed between different subpopulations, which have important implications for treatment strategies.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Editorial Material
Oncology
Jihan K. Osborne, John D. Minna
Summary: In this study, Yin and colleagues utilized Gprc5a knockout genetically engineered mouse models to investigate the cell(s) of origin for lung adenocarcinoma, and suggested that the bronchioalveolar stem cell is the cancer-initiating cell in this model.
Review
Oncology
Jianhui Yang, Omar Aljitawi, Peter Van Veldhuizen
Summary: This review summarizes the recent research progress on the role of CD133 in prostate cancer stem cells (PCSC), including its selective expression in undifferentiated cells, its correlation to treatment resistance, its gene regulation and functional analysis, and its targeted therapy in vitro, in vivo, and in clinical trials.
Article
Biochemistry & Molecular Biology
Yingchen Xia, Chunyan He, Zhi Hu, Zhichao Wu, Yin Hui, Yuan-yuan Liu, Chuanyong Mu, Jianhua Zha
Summary: This study investigates the expression and biological function of YME1L in NSCLC. The results show that YME1L is upregulated in NSCLC tissues and cells, and its knockdown or knockout suppresses cell growth, migration, and induces apoptosis. YME1L dysregulation leads to mitochondrial dysfunction, including depolarization, ROS accumulation, and ATP depletion. Conversely, overexpression of YME1L promotes NSCLC cell proliferation and motility. Akt-S6K1 phosphorylation is affected by YME1L modulation, and YME1L KO-induced anti-NSCLC cell activity can be reversed by Akt1 activation. In vivo, YME1L knockdown inhibits NSCLC xenograft growth. Thus, YME1L has a pro-tumorigenic function in NSCLC.
INTERNATIONAL JOURNAL OF BIOLOGICAL SCIENCES
(2023)
Article
Medicine, General & Internal
Bartlomiej Pawlik, Urszula Smyczynska, Szymon Grabia, Wojciech Fendler, Izabela Drozdz, Katarzyna Babol-Pokora, Katarzyna Kotulska, Sergiusz Jozwiak, Julita Borkowska, Wojciech Mlynarski, Joanna Trelinska
Summary: This study aimed to analyze the serum miRNA profiles in patients with tuberous sclerosis (TSC) treated with sirolimus, and compare them with those previously treated with everolimus. The results showed significant differences in miRNA expression before and after sirolimus treatment. Some miRNAs were dysregulated in the same directions compared with the everolimus group. Pathway analysis indicated the involvement of the Ras and MAPK signaling pathway. The upregulation of specific miRNAs was associated with the downregulation of the mTOR pathway.
JOURNAL OF CLINICAL MEDICINE
(2022)
Article
Medicine, Research & Experimental
Atena Soleimani, Parisa Dadjoo, Amir Avan, Saman Soleimanpour, Majid Rajabian, Gordon Ferns, Mikhail Ryzhikov, Majid Khazaei, Seyed Mahdi Hassanian
Summary: CD133, a CSC marker, is highly expressed in gastric cancer and is associated with malignant behaviors, chemoresistance, and poor prognosis. It also plays an important role in tumor progression and metastasis.
Article
Oncology
Shuying Sun, Austin Zane, Carolyn Fulton, Jasmine Philipoom
Summary: This study investigates hemimethylation in lung cancer for the first time, demonstrating the existence of hemimethylation in lung cells and its potential association with the development of normal and tumor cells. Hemimethylation patterns are diverse and comparable between normal and tumor cells, with identified genes showing differential hemimethylation that may impact gene expression and cell function in non-small cell lung cancer.