期刊
PHARMACEUTICAL RESEARCH
卷 29, 期 2, 页码 362-374出版社
SPRINGER/PLENUM PUBLISHERS
DOI: 10.1007/s11095-011-0554-y
关键词
cancer therapy; cationic lipid; delivery system; gold nanoparticle; siRNA
资金
- Ministry for Health, Welfare and Family Affairs
- World Class University from Ministry of Education, Science and Technology, Republic of Korea [2010-0027955]
Cationic lipid-coated gold nanoparticles were developed for efficient intracellular delivery of therapeutic siRNA. Particle formation was characterized by UV-visible spectroscopy, atomic force microscopy, and dynamic light scattering analysis. Cellular uptake, gene silencing effect, and cytotoxicity were investigated in multiple human cancer cell lines. Nanoparticles had a spherical nanostructure with highly cationic surface charge and could form stable nanosized polyelectrolyte complexes with siRNA via electrostatic interactions; complexes exhibited efficient intracellular uptake and significant gene silencing effect with markedly low cytotoxicity compared to the widely used polycationic carrier, linear polyethyleneimine. We demonstrated that cationic lipid-coated gold nanoparticles could be widely utilized as efficient and safe siRNA nanocarriers for diverse therapeutic and diagnostic applications.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据