Review
Chemistry, Analytical
A. Silge, Karina Weber, D. Cialla-May, L. Mueller-Boetticher, D. Fischer, J. Popp
Summary: The application of Raman spectroscopic methods in life science has made significant progress, driven by advances in instrumentation, miniaturization, and interdisciplinary dialogue. These advancements have brought new possibilities for optical sensing and chemical imaging, contributing to advanced pharmaceutical development and manufacturing. Raman sensing, with its non-destructive and label-free quality testing capabilities, is particularly valuable for process-related aspects in bioprocessing. Additionally, the trend towards personalized medicine has increased the demand for individually tailored medicines, manufactured in public pharmacies.
TRAC-TRENDS IN ANALYTICAL CHEMISTRY
(2022)
Article
Multidisciplinary Sciences
Xiaofan Jiang, Petr Kazarin, Michael D. Sinanis, Ahmad Darwish, Nithin Raghunathan, Alina Alexeenko, Dimitrios Peroulis
Summary: Monitoring product temperature during lyophilization is crucial, and the traditional thermocouple method has limitations. This study developed a new methodology that accurately reconstructs the complete temperature profile using non-invasive temperature monitoring and comprehensive modeling.
SCIENTIFIC REPORTS
(2022)
Article
Biochemistry & Molecular Biology
Charles Farber, A. S. M. Faridul Islam, Endang M. Septiningsih, Michael J. Thomson, Dmitry Kurouski
Summary: Digital farming aims to increase crop yield and reduce environmental impact, requiring the development of sensors. This study demonstrates the potential use of Raman spectroscopy for accurately identifying nutrient components in various rice genotypes, providing a new possibility for seed variety identification.
Article
Crystallography
Julian Brockmeier, Peter Walter Martin Mackwitz, Michael Ruesing, Christof Eigner, Laura Padberg, Matteo Santandrea, Christine Silberhorn, Artur Zrenner, Gerhard Berth
Summary: Potassium titanyl phosphate (KTP) is a nonlinear optical material used for high-power frequency conversion, with less studied domain growth mechanisms compared to widely used lithium niobate. This study utilized confocal Raman spectroscopy to investigate the ferroelectric domain structure in KTP, providing insights for improved domain fabrication.
Article
Biotechnology & Applied Microbiology
A. Graf, J. Lemke, M. Schulze, R. Soeldner, K. Rebner, M. Hoehse, J. Matuszczyk
Summary: Continuous manufacturing is gaining importance in the biopharmaceutical industry for its efficiency, flexibility, and potential to produce higher and more consistent product quality. However, there are challenges in cell culture that require advanced process analytical technologies. Raman spectroscopy has proven to be advantageous for process monitoring and control, as demonstrated in this study using a perfusion process.
FRONTIERS IN BIOENGINEERING AND BIOTECHNOLOGY
(2022)
Article
Chemistry, Multidisciplinary
Zongyu Wu, Binge Deng, Yutong Zhou, Haoqiang Xie, Yumin Zhang, Li Lin, Jian Ye
Summary: In this study, a non-invasive endoscopic technique called surface-enhanced transmission Raman spectroscopy (SETRS) was developed for the precise localization and perioperative surgery navigation of deep lesions. By using ultrabright surface-enhanced Raman spectroscopy (SERS) nanoparticles and a home-built photosafe transmission Raman spectroscopy setup, the authors achieved accurate localization of deep lymph nodes and successfully guided in vivo lymph node biopsy surgery in live rats. This study represents a significant advancement in the translation of TRS techniques for clinical applications and provides insights for the design and implementation of in vivo SERS applications.
Review
Pharmacology & Pharmacy
Eun Ji Kim, Ji Hyeon Kim, Min-Soo Kim, Seong Hoon Jeong, Du Hyung Choi
Summary: Various PAT tools are rapidly advancing to efficiently and precisely monitor various IQAs in the pharmaceutical industry. Therefore, PAT could be a fundamental tool to improve drug product quality in the current QbD and CPV strategies.
Article
Chemistry, Analytical
Panagiota Papaspyridakou, Panagiota Giannoutsou, Malvina G. Orkoula
Summary: The measurement of ethanol and toxic alcohol strengths in beverages and spirits is crucial for health reasons and for identifying adulterated products. Chromatographic and spectroscopic techniques have been used, but their application is time-consuming or requires sample pretreatment. In this study, Raman spectroscopy was used for fast and non-destructive measurements. A through the container method was developed for a non-invasive analysis. The method was validated with calibration curves and used to study the concentrations of alcohol in various products.
Article
Chemistry, Analytical
Benjamin Gardner, Pavel Matousek, Nicholas Stone
Summary: The article introduces a method to correct self-attenuation artifacts in Raman spectroscopy in deep biological tissue analysis. The method successfully reduces Raman spectral intensity variances and distortions in specific peak ratios, with potential to improve detection limits and depth in clinical specimen analysis.
Article
Entomology
Giorgia Giordani, Daniel Whitmore, Stefano Vanin
Summary: Species identification is crucial in scientific studies, and can be done through morphological characters or molecular markers. However, traditional DNA extraction methods are invasive and can damage specimens, which is not suitable for certain samples. This study presents a non-invasive DNA extraction technique for identifying species of flies in the genus Sarcophaga, demonstrating successful results on specimens collected over a long period of time.
Article
Nanoscience & Nanotechnology
Julia Marzi, Ellena Fuhrmann, Eva Brauchle, Verena Singer, Jessica Pfannstiel, Isabelle Schmidt, Hanna Hartmann
Summary: Raman imaging was successfully implemented in this study for molecular-sensitive investigations on bioprinted objects, enabling non-invasive and non-destructive evaluation of embedded cells and discrimination between different cell types and their molecular phenotypes. It is a valuable tool for quality control of bioprinted objects.
ACS APPLIED MATERIALS & INTERFACES
(2022)
Article
Chemistry, Multidisciplinary
Sarah Klingler, Benedikt Bagemihl, Alexander K. Mengele, Simon Kaufhold, Pasi Myllyperkioe, Jussi Ahokas, Mika Pettersson, Sven Rau, Boris Mizaikoff
Summary: Currently, the efficient and economical utilization of photosensitizers and catalysts based on rare earth metals in artificial photosynthesis is a major concern. Although repairing inactivated catalysts is a potential solution, it remains a challenge. The study introduces an innovative in situ technique using non-invasive rotational Raman spectroscopy to accurately monitor initially evolved hydrogen and gas reaction products after active repair, providing valuable insights into the mechanism of active repair during light-driven catalysis and enabling the identification of relevant mechanistic details and innovative repair strategies.
ANGEWANDTE CHEMIE-INTERNATIONAL EDITION
(2023)
Article
Biophysics
Maciej S. Wrobel, Jeong Hee Kim, Piyush Raj, Ishan Barman, Janusz Smulko
Summary: The translation discusses the benefits and challenges of using non-invasive measurement methods, particularly Raman spectroscopy, in medical diagnostics. The authors propose a method to decrease interference from tissue characteristics by leveraging the dynamics of the blood-tissue matrix for enhanced blood analyte signals.
BIOSENSORS & BIOELECTRONICS
(2021)
Article
Chemistry, Analytical
Chenlei Cai, Yujie Liu, Jiayu Li, Lei Wang, Kun Zhang
Summary: In this study, a spectroscopic method based on SLIPSERS was developed for rapid identification of lung cancer. By using SLIPSERS, vibrational fingerprints of serum molecules can be obtained from a small amount of serum sample in minutes. The method is capable of discriminating lung cancer from healthy controls and holds great potential for non-invasive diagnosis.
Article
Spectroscopy
Dalva L. A. Faria, Marta S. Maier, Sara D. Parera, Marisa C. Afonso, Silvia C. Lima, Howell G. M. Edwards
Summary: The chemical investigation of resin-containing museum assets in Brazil can be directly studied by Raman spectroscopy, providing insights into the culture of indigenous peoples and aiding in the establishment of conservation strategies.
JOURNAL OF RAMAN SPECTROSCOPY
(2021)
Article
Instruments & Instrumentation
Christopher Corden, Pavel Matousek, Claudia Conti, Ioan Notingher
Summary: The study investigated the use of time-gated and micro-spatially offset Raman spectroscopy for sub-surface molecular analysis in optically turbid samples. Multiplexed spectral detection allowed fast acquisition of signals for three-dimensional Raman mapping, demonstrating the potential for practical applications in various fields.
APPLIED SPECTROSCOPY
(2021)
Article
Instruments & Instrumentation
Luca Ciaffoni, Pavel Matousek, William Parker, Elin A. McCormack, Hugh Mortimer
Summary: This study presents a generic analytical model for comparing the detection performance of Raman spectrometers based on grating and spatial heterodyne designs. It shows that the SNR ratio between the two types of spectrometers can vary by two orders of magnitude, with spatial heterodyne spectrometers potentially competing with compact grating designs in delivering comparable detection performance in a much smaller footprint.
APPLIED SPECTROSCOPY
(2021)
Article
Chemistry, Analytical
Sara Mosca, Priyanka Dey, Marzieh Salimi, Benjamin Gardner, Francesca Palombo, Nick Stone, Pavel Matousek
Summary: The proposed method utilizes Spatially Offset Raman Spectroscopy (SORS) to estimate the reduced scattering coefficient (mu(s)') of turbid samples, with high accuracy achieved through calibration on a PTFE model sample. Knowledge of mu(s)' in the NIR range is valuable for optimizing SORS illumination and geometric configurations, providing in-depth information about SORS measurements, and benefiting various photon applications in fields such as biomedical, pharmaceutical, security, forensic, and cultural sciences.
ANALYTICAL CHEMISTRY
(2021)
Article
Physics, Multidisciplinary
A. Botteon, M. Realini, C. Colombo, C. Conti, P. Matousek, C. Castiglioni
Summary: This study systematically investigates the micro-SORS capability to characterize a system where an agent diffuses into a turbid matrix, with potential applications in heritage science and other fields. The experiments show that the slope of the Raman intensity decay rate ratio can correlate with the penetration depth of the agent, providing a non-destructive analytical tool for monitoring conservation treatments and other applications involving agent penetration.
EUROPEAN PHYSICAL JOURNAL PLUS
(2021)
Article
Chemistry, Multidisciplinary
Mehrvash Varnasseri, Howbeer Muhamadali, Yun Xu, Paul I. C. Richardson, Nick Byrd, David Ellis, Pavel Matousek, Royston Goodacre
Summary: The study explored the use of Raman spectroscopy and SORS technology for the non-destructive detection of adulteration in extra virgin olive oil. Results demonstrated that these methods have significant potential for rapid and accurate analysis.
APPLIED SCIENCES-BASEL
(2021)
Article
Chemistry, Analytical
Alessandra Botteon, Wang-Hyo Kim, Chiara Colombo, Marco Realini, Chiara Castiglioni, Pavel Matousek, Byung-Man Kim, Tae-Hyuk Kwon, Claudia Conti
Summary: This study demonstrates the ability to monitor the dye depth profile within the light active layer of dye-sensitized solar cells using a non-destructive/non-invasive Raman method, micro-spatially offset Raman spectroscopy (micro-SORS). The results show that micro-SORS can accurately discriminate between homogeneous and inhomogeneous dye depth profiles, and its analysis is reliable compared to destructive time-of-flight secondary ion mass spectrometry measurements.
ANALYTICAL CHEMISTRY
(2022)
Article
Instruments & Instrumentation
Max Dooley, Thomas Paterson, Louise Dexter, Pavel Matousek, Hamid Dehghani, Ioan Notingher
Summary: Spatially offset Raman spectroscopy (SORS) is a powerful technique for subsurface molecular analysis of optically turbid samples. Numerical modeling and experimental validation have shown that offsetting the laser source and detector in opposite directions can significantly improve spectral contrast and signal to noise ratio.
APPLIED SPECTROSCOPY
(2022)
Article
Chemistry, Multidisciplinary
Marzieh Salimi, Sara Mosca, Benjamin Gardner, Francesca Palombo, Pavel Matousek, Nicholas Stone
Summary: The development of new effective cancer treatment methods, especially photothermal therapy using plasmonically resonant metallic nanoparticles, has garnered attention. However, the effectiveness of photothermal therapy can be limited by parameters such as pain sensation experienced by the patient. This review summarizes studies on pain management and enhancing the efficiency of photothermal therapy.
Article
Spectroscopy
Chiara Castiglioni, Alessandra Botteon, Claudia Conti, Matteo Tommasini, Pavel Matousek
Summary: This study introduces an analytical physical description of micro-spatially offset Raman spectroscopy (micro-SORS) experiments in turbid samples and highlights its use for studying the penetration depth of chemical species into a matrix. The study demonstrates the relationship between the Raman intensity ratio and the concentration profiles of the analytes using a physical model and Monte Carlo simulation. The obtained formulas provide insights for interpreting micro-SORS data and comparing different samples.
JOURNAL OF RAMAN SPECTROSCOPY
(2022)
Article
Biochemistry & Molecular Biology
Nai-Hao Yin, Anthony W. Parker, Pavel Matousek, Helen L. Birch
Summary: The purpose of this study is to determine if Raman spectroscopy can detect age-related changes in tendon matrix molecules. The study found that old age groups had significantly higher Raman intensities and fluorescence levels compared to young tendons. Principal component analysis was able to distinguish between different age groups and tendon types. There was also a significant correlation between Raman spectra and levels of advanced glycation end-product (AGE) collagen crosslinks. These findings provide a basis for quantifying age-related chemical modifications to tendon matrix molecules in intact tissue.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Instruments & Instrumentation
Sara Mosca, Kay Sowoidnich, Megha Mehta, William H. Skinner, Benjamin Gardner, Francesca Palombo, Nicholas Stone, Pavel Matousek
Summary: In this work, we propose an advanced CCD read-out combined with SERDS for fast acquisition rates in Raman spectroscopy. The incorporation of a periodic mask and a smaller shift of charge on the CCD allowed for a 10-fold increase in acquisition speed compared to previous designs. The 10 kHz system outperformed earlier variants in different situations, enabling accurate data acquisition from moving samples and under varying ambient light conditions. The high-speed instrument has potential applications in disease diagnosis, accurate data acquisition, and monitoring rapidly evolving Raman signals.
APPLIED SPECTROSCOPY
(2023)
Article
Instruments & Instrumentation
Kay Sowoidnich, Michael Towrie, Pavel Matousek
Summary: This paper presents a holistic approach to addressing the problems of fluorescence interference, sample heterogeneity, and laser-induced sample heating in Raman spectroscopy. Long wavelength shifted excitation Raman difference spectroscopy (SERDS) combined with wide-area illumination and sample rotation is successfully applied for the investigation of selected wood species. The results demonstrate that SERDS is effective in separating the Raman spectroscopic fingerprints of wood species from intense fluorescence interference, and sample rotation with wide-area illumination allows for representative SERDS spectra within a short time.
APPLIED SPECTROSCOPY
(2023)
Article
Chemistry, Analytical
Benjamin Gardner, Pavel Matousek, Nicholas Stone
Summary: The article introduces a method to correct self-attenuation artifacts in Raman spectroscopy in deep biological tissue analysis. The method successfully reduces Raman spectral intensity variances and distortions in specific peak ratios, with potential to improve detection limits and depth in clinical specimen analysis.
Review
Multidisciplinary Sciences
Sara Mosca, Claudia Conti, Nick Stone, Pavel Matousek
Summary: Spatially offset Raman spectroscopy (SORS) is a spectroscopic technique that allows for non-invasive chemical characterization of diffusely scattering materials. It has been widely applied in fields such as disease diagnosis, explosive detection, non-destructive analysis of pharmaceutical products and art objects, with potential future applications.
NATURE REVIEWS METHODS PRIMERS
(2021)
Review
Chemistry, Multidisciplinary
Fay Nicolson, Moritz F. Kircher, Nick Stone, Pavel Matousek
Summary: Advancements in Raman spectroscopy have enabled deep probing of biological tissues through the use of specialist techniques like SORS, achieving depths of up to 5 cm. This progress has opened up new prospects for applications in medical diagnosis and disease monitoring across multiple fields.
CHEMICAL SOCIETY REVIEWS
(2021)