Article
Cell Biology
Eneko Madorran, Andraz Stozer, Zoran Arsov, Uros Maver, Jan Rozanc
Summary: Determining cell viability is a challenge due to uncertainties. A novel method involving the measurement of cell membrane electrical potential shows promising results in directly determining cell viability. The method demonstrates good accuracy when compared to established methods and is compatible with live cell imaging.
Article
Multidisciplinary Sciences
Sue Im Sim, Yuanyuan Chen, Diane L. Lynch, James C. Gumbart, Eunyong Park
Summary: Mitochondria import proteins from the cytosol through the TIM23 complex, which consists of multiple subunits including Tim23 and Tim17. However, the exact structure and mechanism of the TIM23 complex have long been unclear. By using cryo-electron microscopy, researchers have determined the structure of the core TIM23 complex and found that Tim23 and Tim17 form separate cavities rather than a water-filled channel. The results suggest that Tim17 forms the protein translocation path while Tim23 has a structural role, and the nonessential subunit Mgr2 helps facilitate the translocation process by sealing the cavity of Tim17.
Review
Biochemistry & Molecular Biology
Prisha S. Patel, Christopher Castelow, Disha S. Patel, Syamal K. Bhattacharya, Cem Kuscu, Canan Kuscu, Liza Makowski, James D. Eason, Amandeep Bajwa
Summary: Triple negative breast cancer (TNBC) is an aggressive cancer with a high rate of treatment failure and poor prognosis. Mitochondria play key roles in oncogenesis and tumor progression through mechanisms such as altered metabolism, ROS production, and evasion of apoptosis. Studies show that mitochondrial infusion can effectively reduce glycolysis, enhance oxidative phosphorylation, reduce proliferation, and promote apoptosis as anti-tumor therapies.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Medicine, Research & Experimental
Yoshikazu Kuwahara, Kazuo Tomita, Mehryar Habibi Roudkenar, Amaneh Mohammadi Roushandeh, Yusuke Urushihara, Kento Igarashi, Akihiro Kurimasa, Tomoaki Sato
Summary: The study revealed that modulation of mitochondrial function is implicated in the radioresistance phenotype of cancer cells, and radioresistance of clinically relevant radioresistant cells is reversible. Further studies are needed to clarify the mechanisms underlying cancer cell radioresistance.
Article
Biochemistry & Molecular Biology
Amandine Rovini, Kareem Heslop, Elizabeth G. Hunt, Morgan E. Morris, Diana Fang, Monika Gooz, Akos A. Gerencser, Eduardo N. Maldonado
Summary: The study quantified intercellular differences in Delta psi m in human cancer cells and fibroblasts, showing that Delta psi m is more heterogeneous in cancer cells and maintained throughout the cell cycle. The effect of chemical inhibition of the respiratory chain and ATP synthesis varied between cells with different levels of Delta psi m.
Article
Biochemistry & Molecular Biology
Yujia Zhou, Xingtao Zhou, Tao Hong, Wucheng Qi, Ke Zhang, Fang Geng, Shaoping Nie
Summary: Tea polysaccharides (TPs) inhibit the proliferation of colon cancer cells CT26 by targeting lysosomes and inducing apoptosis, mainly through disrupting lysosome function, causing mitochondrial damage, and activating caspase cascade.
Review
Biochemistry & Molecular Biology
Minu Chaudhuri, Anuj Tripathi, Fidel Soto Gonzalez
Summary: Mitochondria play crucial roles in cellular functions, with defects associated with various diseases. Tim50, a key receptor component, is involved in different cellular functions.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Pharmacology & Pharmacy
Tanzeeba Amin, Rajneesh Paul Sharma, Khalid Bashir Mir, Nancy Slathia, Sonali Chhabra, Dolma Tsering, Pankul Kotwal, Madhulika Bhagat, Utpal Nandi, Raman Parkesh, Kamal K. Kapoor, Anindya Goswami
Summary: This study demonstrates that compound 4h binds to AKT-1 and ERK2, inhibiting their activation by blocking ATP binding, leading to apoptosis induction by disrupting mitochondrial membrane potential. It also shows promising anti-tumor activity in mouse models and cancer cells, and exhibits satisfactory pharmacokinetic behavior, making it a potential lead molecule for breast and colorectal cancer treatment by targeting AKT and ERK1/2 kinases simultaneously.
EUROPEAN JOURNAL OF PHARMACOLOGY
(2023)
Article
Oncology
Hydari Masuma Begum, Chelsea Mariano, Hao Zhou, Keyue Shen
Summary: The study demonstrated the role of E-cadherin in negatively regulating the mitochondrial membrane potential of cancer cells in the context of biophysical cues in the tumor microenvironment.
Article
Biology
Marcel G. Genge, Shalini Roy Chowdhury, Vit Dohnalek, Kaori Yunoki, Takashi Hirashima, Toshiya Endo, Pavel Dolezal, Dejana Mokranjac
Summary: This study reveals the coordination mechanism of translocation of proteins across mitochondrial membranes, and finds that both the core and PBD domains of Tim50 play essential roles in this process.
LIFE SCIENCE ALLIANCE
(2023)
Article
Oncology
Hengrui Liu, James P. Dilger, Jun Lin
Summary: Lidocaine inhibits viability and migration of multiple breast cancer cell lines, potentially by regulating the TRPM7 channel. The study findings support the potential clinical application of lidocaine in the treatment of breast cancer.
Article
Biochemistry & Molecular Biology
Nicholas Tripodi, Fotios Sidiroglou, Sarah Fraser, Maja Husaric, Dimitrios Kiatos, Vasso Apostolopoulos, Jack Feehan
Summary: Photobiomodulation (PBM) is a widely used therapeutic intervention, but its underlying mechanisms are still unclear. This research investigated the effects of polarized PBM compared to non-polarized PBM on cellular viability, proliferation, apoptosis, and mitochondrial membrane potential. The results showed that polarized PBM had better effects in promoting cellular proliferation and maintaining mitochondrial membrane potential while reducing cellular apoptosis. This suggests that polarization may have potential in the clinical application of PBM.
JOURNAL OF PHOTOCHEMISTRY AND PHOTOBIOLOGY B-BIOLOGY
(2022)
Article
Multidisciplinary Sciences
Lisa Svartdal Normann, Miriam Ragle Aure, Suvi-Katri Leivonen, Mads Haugland Haugen, Vesa Hongisto, Vessela N. Kristensen, Gunhild Mari Maelandsmo, Kristine Kleivi Sahlberg
Summary: HER2-positive breast cancer patients that do not respond to targeted treatment have a poor prognosis. Through a high-throughput screen, it was found that certain miRNA mimics can sensitize HER2+ breast cancer cells to targeted therapy, with miR-101-5p showing a correlation with better prognosis in patients. This suggests the potential of combining targeted drugs with miRNAs to improve current treatments for HER2+ breast cancers.
SCIENTIFIC REPORTS
(2021)
Article
Biochemistry & Molecular Biology
Edward York, Daniel A. Mcnaughton, Meryem-Nur Duman, Philip A. Gale, Tristan Rawling
Summary: It has been discovered that bisaryl urea compounds can mediate mitochondrial uncoupling, leading to inhibition of breast cancer cell growth. Furthermore, substituting the urea group in bisaryl ureas can alter the activity of the compounds, with different substituents yielding different effects.
Article
Cell Biology
Ertan Kuecueksayan, Anna Sansone, Chryssostomos Chatgilialoglu, Tomris Ozben, Demet Tekeli, Guenel Talibova, Carla Ferreri
Summary: This study assessed the importance of sapienic acid metabolism and signaling cascades in cancer, and found consistent differences among different cell lines. The findings propose a new role of sapienic acid in breast cancer cell metabolism, suggesting potential translational applications for tailored membrane lipid strategies in pharmacological interventions.