Development and Validation of a Procedure for Acyzole Determination in Whole Human Blood

7An HPLC procedure using a HILIC column and UV detection was developed for determination of the carbon monoxide antidote acyzole in human blood after precipitation of proteins with MeCN. The chromatography was performed in isocratic mode over a Nucleodur HILIC column with MeCN:H2O (95:5) mobile phase and detection at 225 nm. The lower limit of quantitation was 116.2 ng/mL; the upper limit, 11,111 ng/mL. Validation of the procedure confirmed that its selectivity, carry-over, linearity, limit of quantitation, precision and accuracy, recovery, and stability satisfied the established guidelines.