期刊
TUBERCULOSIS
卷 95, 期 1, 页码 37-39出版社
CHURCHILL LIVINGSTONE
DOI: 10.1016/j.tube.2014.10.007
关键词
PacBio sequencer; Kurono; PE-PPE; Phyrogenetic tree; Complete genome
资金
- International Health Research from the Ministry of Health, Labor, and Welfare of Japan [24A-103, 26A-103]
- Grants-in-Aid for Scientific Research [25860331] Funding Source: KAKEN
We report the completely annotated genome sequence of Mycobacterium tuberculosis (Zopf) Lehmannand Neumann (ATCC35812) (Kurono), which is a used for virulence and/or immunization studies. The complete genome sequence of M. tuberculosis Kurono was determined with a length of 4,415,078 bp and a G+C content of 65.60%. The chromosome was shown to contain a total of 4,340 protein-coding genes, 53 tRNA genes, one transfer messenger RNA for all amino acids, and 1 rrn operon. Lineage analysis based on large sequence polymorphisms indicated that M. tuberculosis Kurono belongs to the Euro-American lineage (lineage 4). Phylogenetic analysis using whole genome sequences of M. tuberculosis Kurono in addition to 22 M. tuberculosis complex strains indicated that H37Rv is the closest relative of Kurono based on the results of phylogenetic analysis. These findings provide a basis for research using M. tuberculosis Kurono, especially in animal models. (C) 2014 Elsevier Ltd. All rights reserved.
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