Article
Biochemistry & Molecular Biology
Rizwan Qaisar, Gavin Pharaoh, Shylesh Bhaskaran, Hongyang Xu, Rojina Ranjit, Jan Bian, Bumsoo Ahn, Constantin Georgescu, Jonathan D. Wren, Holly Van Remmen
Summary: The study showed that pharmacological activation of SERCA can mitigate sarcopenia phenotype in aging mice, reversing reductions in muscle mass and force generation, and preventing an increase in mitochondrial ROS production. These effects are mediated in part by enhanced cellular energetics through activation of PGC1-alpha, UCP1, HSF1, and APMK, as well as increased regenerative capacity by suppression of MEF2C and p38 MAPK signaling.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Multidisciplinary Sciences
Florence N. Reddish, Cassandra L. Miller, Xiaonan Deng, Bin Dong, Atit A. Patel, Mohammad A. Ghane, Barbara Mosca, Cheyenne McBean, Shengnan Wu, Kyril M. Solntsev, You Zhuo, Giovanni Gadda, Ning Fang, Daniel N. Cox, Angela M. Mabb, Susan Treves, Francesco Zorzato, Jenny J. Yang
Summary: G-CatchER(+) is a green Ca2+ sensor that reports local ER Ca2+ dynamics with improved folding properties and sensitivity. It can detect Ca2+ dynamics in various cell types and differentiate different Ca2+ signaling pathways.
Review
Physiology
Hiroaki Eshima
Summary: Obesity and diabetes can disrupt peripheral insulin resistance in skeletal muscle and lead to loss of muscle size, strength, and physical function, with contractile dysfunction being linked to impaired intracellular Ca2+ concentration regulation. Recent studies have shown that metabolic disorders affect interactions between the SR and mitochondrial networks, altering Ca2+ handling by these organelles. This review highlights the importance of understanding the impact of metabolic disorders on calcium regulation in skeletal muscle for potential therapeutic strategies.
FRONTIERS IN PHYSIOLOGY
(2021)
Article
Biochemistry & Molecular Biology
Enrico Pierantozzi, Peter Szentesi, Cecilia Paolini, Beatrix Dienes, Janos Fodor, Tamas Olah, Barbara Colombini, Dilson E. Rassier, Egidio Maria Rubino, Stephan Lange, Daniela Rossi, Laszlo Csernoch, Maria Angela Bagni, Carlo Reggiani, Vincenzo Sorrentino
Summary: Obscurin plays a significant role in skeletal muscle fibers, affecting muscle function and intracellular Ca2+ dynamics. The diaphragm of Obscn KO mice show prolonged TTP and HRT, decreased sarcoplasmic reticulum Ca2+ release and SERCA reuptake rates, as well as fiber damage, altered sarcomere ultrastructure and M-band disarray after intense exercise.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Cell Biology
Sou Inagaki, Yoshiaki Suzuki, Keisuke Kawasaki, Rubii Kondo, Yuji Imaizumi, Hisao Yamamura
Summary: In this study, the role of mitochondrial fusion protein 2 (Mfn2) in the coupling of sarcoplasmic reticulum (SR) and mitochondria in vascular smooth muscle cells (VSMCs) was examined. It was found that Mfn2 is responsible for tethering mitochondria to SR, which regulates calcium signaling, ATP production, and cell proliferation in VSMCs.
AMERICAN JOURNAL OF PHYSIOLOGY-CELL PHYSIOLOGY
(2022)
Article
Biochemistry & Molecular Biology
Sou Inagaki, Yoshiaki Suzuki, Keisuke Kawasaki, Rubii Kondo, Yuji Imaizumi, Hisao Yamamura
Summary: Mitochondria play a crucial role in cytosolic Ca2+ buffering and energy metabolism. Recent research has shown that Mfn2 regulates Ca2+ signaling by tethering mitochondria and sarco-plasmic reticulum, enhancing mitochondrial function and VSMC proliferation. However, the physiological role of Mfn1 in Ca2+ signaling and mitochondrial function is still unclear.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2023)
Article
Biochemistry & Molecular Biology
Diana Bou-Teen, Nina Kaludercic, David Weissman, Belma Turan, Christoph Maack, Fabio Di Lisa, Marisol Ruiz-Meana
Summary: Excessive mitochondrial ROS production is linked to aging-related heart pathologies, but also serves as a fundamental function in orchestrating signaling pathways that have cardioprotective effects. Mitochondria in cardiac myocytes are abundant and specialized in subcellular populations, with interactions with other organelles influencing their function.
FREE RADICAL BIOLOGY AND MEDICINE
(2021)
Article
Physiology
Alfredo Mijares, Paul D. Allen, Jose R. Lopez
Summary: The study demonstrates that normal aging is associated with increased intracellular Ca2+ and Na+ concentrations, as well as elevated ROS production in skeletal muscle fibers. Treatment with flufenamic acid can reduce these changes, lower levels of plasma interleukin 6 and tumor necrosis factor-alpha, and decrease muscle damage.
FRONTIERS IN PHYSIOLOGY
(2021)
Article
Cardiac & Cardiovascular Systems
Matthew J. Wleklinski, Dmytro O. Kryshtal, Kyungsoo Kim, Shan S. Parikh, Daniel J. Blackwell, Isabelle Marty, V. Ramesh Iyer, Bjorn C. Knollmann
Summary: CASQ2-K180R causes CPVT2 through impairing dynamic buffering of Ca within the SR, which is different from other CASQ2-related forms of CPVT2.
CIRCULATION RESEARCH
(2022)
Review
Cell Biology
Elena Conte, Giorgia Dinoi, Paola Imbrici, Annamaria De Luca, Antonella Liantonio
Summary: SAR is a luminal Ca2+ buffer protein that plays a critical role in Ca2+ regulation and modulation in muscle fibers. It is involved in various physiological functions and has similarities to the well-characterized Ca2+ buffer protein CSQ. Despite the limited targeted studies available, SAR deserves attention as an important yet underinvestigated protein.
Article
Cardiac & Cardiovascular Systems
Maren E. Arnold, Wolfgang R. Dostmann, Jody Martin, Michael J. Previs, Bradley Palmer, Martin LeWinter, Markus Meyer
Summary: This study introduces a novel biosensor that can visualize the interaction between SERCA2a and phospholamban (PLB). The biosensor combines SERCA2a, a circularly permutated green fluorescent protein, and PLB into one recombinant protein (SGP). Activation of proteinkinase A leads to phosphorylation of the PLB domain, resulting in a significant increase in fluorescence yield for real-time monitoring of SERCA2a and PLB interactions in cells.
AMERICAN JOURNAL OF PHYSIOLOGY-HEART AND CIRCULATORY PHYSIOLOGY
(2021)
Article
Physiology
Adan Dagnino-Acosta, Agustin Guerrero-Hernandez
Summary: PKC inhibitors stimulate Ca2+ release from internal stores by increasing SR Ca2+ leak mediated by translocon activation. This increased leak does not deplete the Ca2+ store, but rather compensates by increasing SERCA pump activity, leading to a new steady-state level. This change also results in increased activity of high conductance, Ca2+-sensitive potassium channels.
FRONTIERS IN PHYSIOLOGY
(2022)
Article
Cell Biology
Daniela Rossi, Stefania Lorenzini, Enrico Pierantozzi, Filip Van Petegem, David Osamwonuyi Amadsun, Vincenzo Sorrentino
Summary: Junctin is a transmembrane protein that plays a role in striated muscles by interacting with calsequestrin and the ryanodine receptor. Our study found that junctin and calsequestrin co-localize in the endoplasmic reticulum and triads, and identified specific regions of junctin that can bind calsequestrin and the RyR. Deletion of these regions affects junctin's localization at the junctional SR.
JOURNAL OF CELL SCIENCE
(2022)
Article
Biochemistry & Molecular Biology
Monika Sztretye, Zoltan Singlar, Nyamkhuu Ganbat, Dana Al-Gaadi, Kitti Szabo, Zoltan Marton Kohler, Laszlo Dux, Aniko Keller-Pinter, Laszlo Csernoch, Peter Szentesi
Summary: The remodelling of the extracellular matrix plays a crucial role in skeletal muscle development and regeneration. The decreased expression of Syndecan-4 leads to reduced muscle performance and altered calcium homeostasis in mice. These effects are observed at both young and aged stages.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Multidisciplinary Sciences
Zsuzsanna E. Magyar, Judit Hevesi, Linda Groom, Robert T. Dirksen, Janos Almassy
Summary: The T4706M mutation in the RyR1 protein is associated with muscle weakness and alters channel function, leading to a complex mechanism of disease pathogenesis.
SCIENTIFIC REPORTS
(2023)