Article
Pharmacology & Pharmacy
Ellen Emi Kato, Luciana Araujo Pimenta, Maira Estanislau Soares de Almeida, Vanessa Olzon Zambelli, Marinilce Fagundes dos Santos, Sandra Coccuzzo Sampaio
Summary: The study demonstrated that CTX inhibits major events involved in angiogenesis, such as tube formation and cell adhesion, proliferation, and migration. These actions were particularly effective against tumor stimuli, highlighting the importance of CTX's anti-angiogenic action in inhibiting tumor progression.
FRONTIERS IN PHARMACOLOGY
(2021)
Article
Engineering, Biomedical
Samuel Alkmin, Manish S. Patankar, Paul J. Campagnola
Summary: Ovarian cancer is the deadliest gynecological cancer due to poor screening and imaging tools as well as limited understanding of the tumor microenvironment. This study used multiphoton excited fabrication to create orthogonal models representing the collagen matrix morphology and stiffness of normal ovarian stroma and high grade serous ovarian cancers. The study found that fiber morphology and matrix stiffness play important roles in migration and cytoskeletal dynamics of normal and cancer cells, and cell-specific differences highlight the importance of presenting both morphology and stiffness cues.
ACTA BIOMATERIALIA
(2022)
Article
Materials Science, Biomaterials
Adrian A. Shimpi, Erik D. Williams, Lu Ling, Tigist Tamir, Forest M. White, Claudia Fischbach
Summary: This study investigates tumor cell migration on fibrotic ECM using a cell-derived matrix (CDM) model and phosphotyrosine phosphoproteomics. The results demonstrate that tumor cells cultured on CDMs migrate faster and in a more directional manner, which correlates with decreased cell spreading and increased cell elongation. The study also suggests that LYN may be differentially regulated and involved in regulating tumor cell migration on CDMs.
ACS BIOMATERIALS SCIENCE & ENGINEERING
(2023)
Article
Engineering, Biomedical
Anita Akbarzadeh Solbu, David Caballero, Spyridon Damigos, Subhas C. Kundu, Rui L. Reis, Oyvind Halaas, Aman S. Chahal, Berit L. Strand
Summary: Cell migration plays a crucial role in various biological processes, and recent advancements in materials science have allowed for the development of biomimetic in vitro assays to study cell migration. Three-dimensional hydrogels have been particularly useful in mimicking the extracellular environment and studying cell migration in a more physiologically relevant manner. This article provides an overview of the different types of hydrogels and their characteristics, as well as established methods for cell migration assays and tracking in three-dimensional environments.
MATERIALS TODAY BIO
(2023)
Article
Biochemistry & Molecular Biology
Asif Amin, Asif Lone, Faizah Farooq, Umer Majeed Wani, Fizallah Kawoosa, Raies A. A. Qadri
Summary: The interaction between Tetranectin and plasminogen is crucial for extracellular matrix degradation, enabling tumor cell invasion and metastasis. This study aimed to target the carbohydrate recognition domain (CRD) of Tetranectin using natural bioactive compounds to inhibit its interaction with the Kringle-4 domain of plasminogen. Diosgenin and scytonemin exhibited favorable interactions with CRD, but diosgenin showed increased stability in the CRD binding pocket compared to scytonemin. Diosgenin was found to exert a pronounced anti-proliferative effect on breast cancer cells and reduce their migration and invasiveness.
JOURNAL OF BIOMOLECULAR STRUCTURE & DYNAMICS
(2023)
Article
Engineering, Biomedical
Duo Zhang, Yaqi Sheng, Nicholas Piano, Theresa Jakuszeit, Edward Jonathan Cozens, Lingqing Dong, Alexander K. Buell, Andreas Pollet, Iek Man Lei, Wenyu Wang, Eugene Terentjev, Yan Yan Shery Huang
Summary: Cell migration is regulated by the fibrillar structure of the extracellular matrix. Cells dynamically adjust their shape and migration trajectory in response to different fibrillar environments. The average step speed of cells is minimally affected by the global pattern of the fibers, but a switch in behavior is observed when the pattern features approach the upper limit of the cell body's minor axis.
Article
Multidisciplinary Sciences
Florian Geiger, Lukas G. Schnitzler, Manuel S. Brugger, Christoph Westerhausen, Hanna Engelke
Summary: This study investigates the influence of fiber orientation on invasion from a cancer cell spheroid in a collagen matrix. The results demonstrate a strong preference for invasion along radial fibers rather than tangential fibers.
Article
Biochemical Research Methods
Marco Ruscone, Arnau Montagud, Philippe Chavrier, Olivier Destaing, Isabelle Bonnet, Andrei Zinovyev, Emmanuel Barillot, Vincent Noël, Laurence Calzone
Summary: In this study, a multiscale model is used to investigate the invasion process of tumor cells. The model combines agent-based modeling and continuous time Markov processes applied on Boolean network models. By considering both spatial information from the agent-based simulation and intracellular regulation from the Boolean model, the model aims to study different modes of cell migration and predict means to block it. The multiscale model successfully reproduces single and collective migration processes and is validated on published experiments on cell invasion, suggesting possible targets to block invasive tumor phenotypes through in silico experiments.
Article
Multidisciplinary Sciences
Matthew R. Zanotelli, Jian Zhang, Ismael Ortiz, Wenjun Wang, Neil C. Chada, Cynthia A. Reinhart-King
Summary: The study found that changes in collagen density are correlated with cell migration ability and bioenergetics, with significantly different effects observed in highly motile cells. These results reveal a link between matrix mechanics, migratory phenotype, and bioenergetics.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2022)
Review
Cell Biology
Anna Pawluchin, Milos Galic
Summary: This review discusses the differences in cell migration between 3D and 2D environments, and how these differences affect subcellular structures and motion patterns.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2022)
Article
Nanoscience & Nanotechnology
Liu Shi, Lin Wang, Xuemei Ma, Mohammed Jalalah, Saeed A. Alsareii, Tao Gao, Farid A. Harraz, Genxi Li
Summary: The study introduces a novel method called the electrochemical trans-channel assay for efficiently evaluating tumor cell invasiveness. By using a bioinspired extracellular matrix degradation model to construct the electrochemical apparatus, the invasive capability of tumor cells can be sensitively evaluated, providing a faster and more convenient alternative to traditional migration methods. This assay also allows for quantification of the invasion process, offering a promising approach for clinical assessment or prognostic applications of tumor metastasis.
ACS APPLIED MATERIALS & INTERFACES
(2021)
Article
Polymer Science
Li Yao, Kimmy Tran, Diana Nguyen
Summary: Glioma cells produce an increased amount of collagen, which affects the invasion ability of tumor cells. This study tested the migration of glioma cells, fetal astrocytes, and adult astrocytes in a 3D collagen matrix with different collagen concentrations, and found higher motility in a low concentration of collagen hydrogel. It was also discovered that glioma cells responded to direct current electric field stimulation and migrated to the anodal pole.
Article
Engineering, Biomedical
Harrison L. Hiraki, Daniel L. Matera, William Y. Wang, Eashan S. Prabhu, Zane Zhang, Firaol Midekssa, Anna E. Argento, Johanna M. Buschhaus, Brock A. Humphries, Gary D. Luker, Abdon Pena-Francesch, Brendon M. Baker
Summary: The peritumoral stroma is a complex tissue that influences the migration mode of transformed epithelial cells during metastatic spread. Understanding the role of cues from the stromal microenvironment in regulating migration mode has prognostic and therapeutic value.
ACTA BIOMATERIALIA
(2023)
Article
Multidisciplinary Sciences
Mingxi Yao, Ajay Tijore, Delfine Cheng, Jinyuan Vero Li, Anushya Hariharan, Boris Martinac, Guy Tran Van Nhieu, Charles D. Cox, Michael Sheetz
Summary: Mechanosensing is crucial for various physiological processes, and two major mechanosensing systems, focal adhesions and mechanosensitive ion channels, can convert mechanical features of the microenvironment into biochemical signals. Interestingly, it has been discovered that the mechanosensitive calcium-permeable channel Piezo1, previously thought to be diffusive on plasma membranes, binds to matrix adhesions in a force-dependent manner, thereby promoting cell spreading, adhesion dynamics, and calcium entry in normal cells but not in most cancer cells.
Article
Biochemistry & Molecular Biology
Fabian Graf, Patrick Horn, Anthony D. Ho, Michael Boutros, Christian Maercker
Summary: This study found that mesenchymal stromal cells from human bone marrow may induce migratory activity in cancer cells, and extracellular matrix proteins could be potential drivers of cancer cell migration. Knocking down the gene encoding beta 1 integrin led to inhibition of cancer cell migration, suggesting that integrin signaling plays a crucial role in metastasis.
Article
Fisheries
S. Shukla, S. K. Shukla, R. Sharma, A. Kumar
JOURNAL OF APPLIED ICHTHYOLOGY
(2014)
Meeting Abstract
Peripheral Vascular Disease
Pooja Singh, Alok Singh, Anand Narain Srivastava, Rolee Sharma, Seema Singh, Siddhartha Chandel, Rajveer Singh Chauhan
JOURNAL OF HYPERTENSION
(2018)
Article
Environmental Sciences
P. Arya, P. K. Pradhan, R. Sharma, N. Sood
JOURNAL OF ENVIRONMENTAL BIOLOGY
(2019)
Article
Biochemistry & Molecular Biology
Akanksha Sharma, M. Hayatul Islam, Nida Fatima, Tarun K. Upadhyay, M. Kalim A. Khan, Upendra N. Dwivedi, Rolee Sharma
MOLECULAR BIOLOGY REPORTS
(2019)
Article
Biotechnology & Applied Microbiology
Tarun K. Upadhyay, Nida Fatima, Akanksha Sharma, Deepak Sharma, Rolee Sharma
ARTIFICIAL CELLS NANOMEDICINE AND BIOTECHNOLOGY
(2019)
Article
Developmental Biology
P. Arya, P. K. Pradhan, Anutosh Paria, R. Sharma, Dev K. Verma, Ravindra, G. Rathore, N. Sood
GENE EXPRESSION PATTERNS
(2019)
Article
Medicine, Research & Experimental
Nida Fatima, Tarun Upadhyay, Firoz Ahmad, Md Arshad, Mohammad Amjad Kamal, Deepak Sharma, Rolee Sharma
Summary: Certain microparticles, such as YDGP, are capable of inducing NOX-2-dependent autophagy and antimicrobial activity, showing potential as therapeutic agents in the future.
Article
Immunology
Amit K. Singh, Rahul K. Verma, Jatinder Kaur Mukker, Awadh B. Yadav, Pavan Muttil, Rolee Sharma, Mradul Mohan, Atul K. Agrawal, Anuradha Gupta, Anil K. Dwivedi, Pushpa Gupta, Umesh D. Gupta, Uthirappan Mani, Bhushan P. Chaudhari, Ramesh C. Murthy, Sharad Sharma, Smrati Bhadauria, Sarika Singh, Srikanta Kumar Rath, Amit Misra
Summary: The study demonstrates the efficacy and safety of biodegradable polymeric particles containing INH and RFB as an adjunct to oral chemotherapy in treating tuberculosis, showing the ability to eliminate infection and prevent relapse.
Article
Chemistry, Medicinal
Kratika Singh, Akanksha Sharma, Tarun Kumar Upadhyay, Mohammad Hayat-Ul-Islam, M. Kalim A. Khan, Upendra N. Dwivedi, Rolee Sharma
Summary: The present study aimed to find novel potent inhibitors against M.tb. GlgE target. A natural compound, asiatic acid, was identified as a promising anti-mycobacterial agent through virtual screening, molecular docking, molecular dynamics simulation, and in vitro analysis.
CURRENT COMPUTER-AIDED DRUG DESIGN
(2022)
Article
Infectious Diseases
Kratika Singh, Niharika Pandey, Firoz Ahmad, Tarun Kumar Upadhyay, Mohammad Hayatul Islam, Nawaf Alshammari, Mohd Saeed, Lamya Ahmed Al-Keridis, Rolee Sharma
Summary: This study identified a natural compound gravacridonediol as a potential inhibitor of the key target enzyme InhA of Mycobacterium tuberculosis. Pharmacokinetics and molecular docking analyses were performed on compounds from Ruta graveolens and citrus plants, resulting in the discovery of gravacridonediol with higher binding efficiency than the known inhibitor triclosan.
Article
Pharmacology & Pharmacy
Bikash Sarmah, Pushpendra Kumar Shukla, Manoj Kumar Sarma, Rolee Sharma
Summary: Pueraria tuberosa is a medicinal plant commonly found in Southeast Asia, mainly in India. It is used in traditional medicine for various ailments and contains high levels of isoflavones and other phytochemicals. The study found that it has significant antidiabetic, anti-inflammatory, and antioxidant activities.
ANNALS OF PHYTOMEDICINE-AN INTERNATIONAL JOURNAL
(2022)
Article
Genetics & Heredity
Shubhra Shukla, Sanjeev Kumar Shukla, Rolee Sharma
Meeting Abstract
Oncology
P. Singh, A. N. Srivastava, A. Singh, R. Sharma
Article
Biology
Tarun K. Upadhyay, Nida Fatima, Deepak Sharma, V. Saravanakumar, Rolee Sharma
Article
Mathematical & Computational Biology
Rashi Srivastava, Salman Akthar, Rolee Sharma, Sanjay Mishra