期刊
PEST MANAGEMENT SCIENCE
卷 67, 期 12, 页码 1557-1561出版社
WILEY-BLACKWELL
DOI: 10.1002/ps.2214
关键词
carboxylic acid amide; diagnosis; fungicide resistance; mandipropamid; PCR-RFLP; Plasmopara viticola
BACKGROUND: The occurrence of carboxylic acid amide (CAA)-fungicide-resistant Plasmopara viticola populations is becoming a serious problem in the control of grapevine downy mildew worldwide. RESULTS: The authors have developed a method, which utilises PCR-RFLP, for the rapid detection of resistance to the CAA fungicide mandipropamid in P. viticola populations. With this method, a glycine-to-serine substitution at codon 1105 of the cellulose synthase gene PvCesA3 of CAA-fungicide-resistant P. viticola was easily detected, although no resistant P. viticola was detected from 398 isolates in Japan. CONCLUSION: It is proposed that the PCR-RFLP method is a reliable tool for the rapid detection of CAA-fungicide-resistant P. viticola isolates. Only 4 h was required from the sampling of symptoms to the phenotyping of fungicide resistance. (C) 2011 Society of Chemical Industry
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