Article
Biotechnology & Applied Microbiology
Hyeonseek Park, Doo Ri Kim, Areum Shin, Eunjung Jeong, Sohee Son, Jin-Hyun Ahn, So Yoon Ahn, Suk-Joo Choi, Soo-young Oh, Yun Sil Chang, Yae-Jean Kim, Minhee Kang
Summary: Congenital CMV infection is a common cause of hearing loss and neurodevelopmental impairment in newborns. A robust LAMP assay was developed for large-scale screening of newborns, providing higher sensitivity and shorter detection time compared to qPCR. The colorimetric LAMP assay can also be used for point-of-care testing in low-resource environments.
APPLIED MICROBIOLOGY AND BIOTECHNOLOGY
(2023)
Article
Pharmacology & Pharmacy
Stefan Weinberger, Christoph Steininger
Summary: Polymerase chain reaction (PCR) methods are effective in monitoring viral load in CMV patients, but may overestimate viral load in patients receiving Letermovir (LMV) treatment. This study compared the effects of LMV and Ganciclovir (GCV) treatment on viral DNA levels in infected cells. Results showed that DNase treatment had different effects on cell lysates and cell culture supernatants, suggesting that concatemeric DNA accumulation may confound LMV efficacy evaluation through qPCR.
ANTIVIRAL RESEARCH
(2022)
Article
Pediatrics
Megan H. Pesch, Mark R. Schleiss
Summary: This article reviews recent developments in the understanding of congenital cytomegalovirus (cCMV), including epidemiology and transmission, diagnostic strategies, newborn screening programs, therapeutics, and vaccine trials. It discusses how this knowledge informs the pathophysiology of cCMV and directs strategies for improving outcomes and quality of life for infected children. The article also provides an update on the epidemiology of cCMV in the United States, evolving understanding of maternal-fetal transmission, enhanced screening approaches, and recognition of neonatal and long-term sequelae. Finally, it reviews the current landscape of pediatric cCMV research and gives recommendations for future investigation.
Review
Oncology
Inti Peredo-Harvey, Afsar Rahbar, Cecilia Soderberg-Naucler
Summary: The study found that human cytomegalovirus (HCMV) is highly prevalent in glioblastomas and optimized immunohistochemistry techniques are required to detect it, although there are discrepancies in detection methods and results using different techniques.
Article
Pediatrics
Megan H. Pesch, Phoebe Danziger, Lainie Friedman Ross, Armand H. Matheny Antommaria
Summary: This article discusses the ethical and clinical implications of newborn cCMV screening programs. On one hand, early cCMV detection can provide developmental advantages and support broader treatment approaches. On the other hand, there are shortcomings in available testing platforms and concerns about overdiagnosis and overtreatment. The article also mentions the risks of parental anxiety and vulnerable child syndrome as barriers to screening.
Article
Biochemical Research Methods
Weidu Song, Chuanhao Zhang, Huichao Lin, Taiyi Zhang, Haixia Liu, Xiaowen Huang
Summary: The proposed real-time rotary PCR reaction system (RRP) meets the requirements of low cost, low-power consumption, potential for miniaturization, and portability. Experimental and theoretical results demonstrate its feasibility in the rapid quantitative detection of Pseudomonas aeruginosa and have broad application prospects in bacteria detection and food safety.
Article
Biochemical Research Methods
Shaolei Huang, Yiquan An, Bangchao Xi, Xianglian Gong, Zhongfu Chen, Shan Shao, Shengxiang Ge, Jun Zhang, Dongxu Zhang, Ningshao Xia
Summary: Nucleic acid detection is a method that directly identifies the presence of pathogenic microorganisms and has advantages of high sensitivity, commendable specificity, and a short window period. A real-time PCR system based on multiple temperature zones was proposed to solve the time-consuming problem, enabling fast detection speed and high sensitivity under extreme conditions.
Article
Immunology
Justyna Czech-Kowalska, Dominika Jedlinska-Pijanowska, Beata Kasztelewicz, Magdalena Klodzinska, Aleksandra Pietrzyk, Eliza Sarkaria, Dorota Dunin-Wasowicz, Kinga Gradowska, Anna Niezgoda, Dariusz Gruszfeld, Anna Dobtsanska
Summary: A positive CSF-CMV-PCR result is associated with prematurity, microcephaly, seizures, sensorineural hearing loss, and other symptoms in infants with cCMV. Cystic lesions and calcifications on MRI are significant predictors of positive CSF-CMV-PCR results.
PEDIATRIC INFECTIOUS DISEASE JOURNAL
(2021)
Article
Pediatrics
Sheila C. Dollard, Maggie Dreon, Nelmary Hernandez-Alvarado, Minal M. Amin, Phili Wong, Tatiana M. Lanzieri, Erin A. Osterholm, Abbey Sidebottom, Sondra Rosendahl, Mark T. McCann, Mark R. Schleiss
Summary: This study demonstrates relatively high analytical sensitivity for DBS compared with previous studies that performed population-based screening. As more sensitive DNA extraction and PCR methods continue to emerge, DBS-based testing should remain under investigation as a potential low-cost, high-throughput option for cCMV screening.
Article
Virology
K. Shahar-Nissan, M. Oikawa Tepperberg, E. Mendelson, E. Bilavsky
Summary: This study assessed the clinical characteristics of children retrospectively diagnosed with congenital cytomegalovirus (cCMV) using dried blood-spot (DBS) polymerase chain reaction (PCR) testing and reported missed opportunities for early diagnosis and treatment. Universal newborn screening for cCMV could have prevented poor hearing and developmental outcomes in some cases.
JOURNAL OF CLINICAL VIROLOGY
(2022)
Article
Multidisciplinary Sciences
Jae Jong Kim, Hyoung-Min Park, A. Young Kyoung, In Kyung Park, Si-Kyu Lim, Byoung Chul Park
Summary: Genetic mutations like SNP are common in genetic disorders and cancers, and PCR methods are widely used for SNP detection. The specifically designed PCR platform STexS can efficiently distinguish small amounts of SNP mixed in normal DNA, aiding in targeting potential SNPs or genetically mutated biomarkers in human clinical samples.
SCIENTIFIC REPORTS
(2021)
Article
Ophthalmology
Yukiko Terada, Toshikatsu Kaburaki, Hiroshi Takase, Hiroshi Goto, Satoko Nakano, Yoshitsugu Inoue, Kazuichi Maruyama, Kazunori Miyata, Kenichi Namba, Koh-Hei Sonoda, Yutaka Kaneko, Jiro Numaga, Masaya Fukushima, Noe Horiguchi, Mitsunao Ide, Fumie Ehara, Dai Miyazaki, Eiichi Hasegawa, Manabu Mochizuki
Summary: This study aimed to determine the clinical characteristics of anterior uveitis caused by HSV, VZV, and CMV, finding significant differences between CMV-associated AU and the other two types. Symptoms like ocular hyperemia, pain, blurring of vision, and signs like keratic precipitates were more common in HSV-AU and VZV-AU compared to CMV-AU.
AMERICAN JOURNAL OF OPHTHALMOLOGY
(2021)
Article
Otorhinolaryngology
Sang-Yeon Lee, Heong Won Jeon, So-Yeon Ahn, Seung-Ha Oh, Bong Jik Kim, Byung Yoon Choi
Summary: This study aims to explore the diagnostic role of viral markers for cytomegalovirus (CMV) in children with sensorineural hearing loss (SNHL) after the critical period. The study found that CMV PCR positivity, PCR titers, and culture positivity successfully differentiated the group with congenital CMV-related SNHL from other groups. A hypothetical formula was developed to predict cCMV infections using logistic regression analysis.
INTERNATIONAL JOURNAL OF PEDIATRIC OTORHINOLARYNGOLOGY
(2023)
Article
Neurosciences
Di Qu, Die Hu, Jing Zhang, Guodong Yang, Jia Guo, Dongfang Zhang, Chao Qi, Haitao Fu
Summary: This study aimed to identify differentially expressed ferroptosis-related genes (DE-FRGs) in human acute spinal cord injury (SCI) and validate their expression in non-SCI and SCI patients. The results showed 41 DE-FRGs associated with reactive oxygen species, redox reactions, and ferroptosis pathways. The hub DE-FRGs, including TLR4, STAT3, and HMOX1, were validated in clinical blood samples, indicating their potential as therapeutic targets for SCI.
MOLECULAR NEUROBIOLOGY
(2023)
Article
Chemistry, Multidisciplinary
Sudheendran Mavila, Heidi R. Culver, Alex J. Anderson, Tania R. Prieto, Christopher N. Bowman
Summary: An athermal approach using self-immolative thioester linked nucleic acids (TENA) for mRNA enrichment from total RNA has shown comparable performance to a commercial kit. The method is compatible with downstream applications such as RT-qPCR for gene expression analysis.
ANGEWANDTE CHEMIE-INTERNATIONAL EDITION
(2022)
