Article
Biochemistry & Molecular Biology
Constanze Banz-Jansen, Laureen P. Helweg, Barbara Kaltschmidt
Summary: Endometrial cancer is a common malignant disease in women, and targeting cancer stem cells and immunotherapy could improve prognosis and prevent cancer progression.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Oncology
Manon Douyere, Caifeng Gong, Mylene Richard, Nadia Pellegrini-Moise, Joel Daouk, Julien Pierson, Pascal Chastagner, Cedric Boura
Summary: This study demonstrates that targeting NRP1 with MR438 could be a potential new strategy to limit the progression of medulloblastoma by decreasing the number of stem cells and reducing the radiation dose.
CANCER CELL INTERNATIONAL
(2022)
Article
Plant Sciences
Wenhao Liao, Lanlan Zhang, Xian Chen, Juyi Xiang, Qiao Zheng, Nianzhi Chen, Maoyuan Zhao, Gang Zhang, Xiaolin Xiao, Gang Zhou, Jinhao Zeng, Jianyuan Tang
Summary: This review examines the effects and mechanisms of phytochemicals on colorectal cancer stem cells (CSCs) and summarizes different phytochemicals based on their mechanisms of action. It was found that phytochemicals can inhibit the self-renewal, migration, invasion, chemoresistance, and induce apoptosis and differentiation of CSCs through regulating various signaling pathways. These findings suggest that phytochemicals have potential as novel therapeutic agents for colorectal CSCs.
Review
Cell & Tissue Engineering
Kai Song, Maryam Farzaneh
Summary: Breast cancer stem cells play a critical role in the metastasis and aggressiveness of breast cancer, making them a suitable target for treatment. Signaling pathways such as Wnt, NF kappa B, and miRNAs are key players in regulating BCSC self-renewal and metastasis. Targeting these pathways may offer a novel therapeutic approach for breast cancer diagnosis and therapy.
STEM CELL RESEARCH & THERAPY
(2021)
Article
Clinical Neurology
Francesca Nazio, Agnese Po, Luana Abballe, Claudio Ballabio, Francesca Diomedi Camassei, Matteo Bordi, Antonio Camera, Simona Caruso, Ignazio Caruana, Marco Pezzullo, Caterina Ferraina, Giacomo Milletti, Matteo Gianesello, Sofia Reddel, Carmen Dolores De Luca, Donatella Ceglie, Sara Marinelli, Silvia Campello, Elena Papaleo, Evelina Miele, Antonella Cacchione, Andrea Carai, Maria Vinci, Enrico Velardi, Biagio De Angelis, Luca Tiberi, Concetta Quintarelli, Angela Mastronuzzi, Elisabetta Ferretti, Franco Locatelli, Francesco Cecconi
Summary: Medulloblastoma (MB) is a childhood malignant brain tumor with four main subgroups, among which patients with enhanced levels of the c-MYC oncogene (MBGroup3) have the poorest prognosis. Research has identified a previously unrecognized role of the pro-autophagy factor AMBRA1 in regulating MB, with its expression being dependent on c-MYC levels and correlating with poor prognosis in MBGroup3 patients. Knockdown of AMBRA1 reduces MBGroup3 stem cells' potential, growth, and migration, indicating its significant impact on the disease.
ACTA NEUROPATHOLOGICA
(2021)
Review
Biochemistry & Molecular Biology
Andrea Angius, Antonio Mario Scanu, Caterina Arru, Maria Rosaria Muroni, Vincenzo Rallo, Giulia Deiana, Maria Chiara Ninniri, Ciriaco Carru, Alberto Porcu, Giovanna Pira, Paolo Uva, Paolo Cossu-Rocca, Maria Rosaria De Miglio
Summary: Colorectal cancer stem cells (CCSCs) with stemness behaviors play a crucial role in tumor progression and therapy resistance, associated with genetic changes in signaling pathways. Dysregulated CCSC-related miRNAs modulate stemness features and functions, involving cell cycle gene expression, epithelial-mesenchymal transition, and drug-resistance mechanisms.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Cell Biology
Ava Nasrolahi, Shirin Azizidoost, Klaudia Radoszkiewicz, Sajad Najafi, Farhoodeh Ghaedrahmati, Omid Anbiyaee, Seyed Esmaeil Khoshnam, Maryam Farzaneh, Shahab Uddin
Summary: Glioma is a common malignant brain tumor that arises from glial tissue. Glioma cancer stem cells (GCSCs) have been identified as playing important roles in tumor initiation and metastasis. Targeting GCSCs and their associated signaling pathways, as well as non-coding RNAs, could be a novel strategy for glioma treatment. In this review, critical signaling pathways involved in GCSC tumorigenesis and invasiveness are summarized.
CELLULAR SIGNALLING
(2023)
Review
Oncology
Lei Wang, Zeng Jin, Rohan P. Master, Chandra K. Maharjan, Madison E. Carelock, Tiffany B. A. Reccoppa, Myung-Chul Kim, Ryan Kolb, Weizhou Zhang
Summary: This article summarizes the important features of breast cancer stem cells, major signaling pathways, and cellular interactions within the tumor microenvironment, highlighting the significance of BCSCs in breast cancer and potential therapeutic strategies.
Review
Cell Biology
Alejandro Ordaz-Ramos, Olivia Tellez-Jimenez, Karla Vazquez-Santillan
Summary: Breast cancer stem cells (BCSCs) are a specific subpopulation of cells capable of self-renewal and differentiation into diverse tumor cells. The involvement of cancer stem cells in treatment resistance and cancer recurrence is well established. Specific signaling pathways, such as Wnt/beta-catenin, NF-kappa B, Notch, Hedgehog, TGF-beta, and Hippo, play crucial roles in regulating the self-renewal ability of cancer stem cells. Targeting these signaling pathways holds promise as a therapeutic strategy for combating breast cancer aggressiveness. This review discusses the main signaling pathways involved in maintaining the self-renewal ability of BCSCs and highlights current strategies to disrupt stemness-related signaling molecules.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2023)
Review
Cell Biology
Yao-An Shen, Chang-Cyuan Chen, Bo-Jung Chen, Yu-Ting Wu, Jiun-Ru Juan, Liang-Yun Chen, Yueh-Chun Teng, Yau-Huei Wei
Summary: Cancer stem cells (CSCs) play a crucial role in cancer treatment and progression, with unique metabolic characteristics that differ from normal cells. Studying the metabolic pathways that CSCs rely on can provide new opportunities and methods for cancer treatment.
Review
Oncology
Emilia Bevacqua, Jasmin Farshchi, Maria Victoria Niklison-Chirou, Paola Tucci
Summary: Medulloblastoma is the most common malignant brain tumor in children, originating in the cerebellum due to DNA alterations. miRNAs play important roles in medulloblastoma, either helping cell proliferation or inhibiting it to promote cell differentiation. Further research on the role of miRNAs in the four molecular subgroups of medulloblastoma is ongoing to provide potential therapeutic applications.
Review
Oncology
Giuliana Catara, Daniela Spano
Summary: Cancer is a global public health issue with increasing cases expected by 2040, and cancer stem cells (CSCs) play a crucial role in treatment due to their resistance to conventional therapies. It is crucial to research and design alternative combinatorial therapeutic strategies to combat this resistance.
FRONTIERS IN ONCOLOGY
(2021)
Article
Oncology
Zhenbo Tu, Antoine E. Karnoub
Summary: Mesenchymal stem/stromal cells (MSCs) are a heterogeneous population of fibroblastic progenitor cells that reside in multiple tissues around the body and have the ability to differentiate into various connective tissue cells. They play critical regulatory roles in breast tumor development and progression, participating in multiple aspects of the process.
SEMINARS IN CANCER BIOLOGY
(2022)
Review
Cell Biology
Raul Fernandez Perez, Juan Ramon Tejedor, Agustin Fernandez Fernandez, Mario Fernandez Fraga
Summary: Aging and cancer are clearly associated processes, and epigenetic mechanisms play a role in connecting the two phenomena. However, recent studies have shown significant differences in DNA methylation loss between aging and cancer. The availability of genome-wide epigenetic data allows for systematic studies to clarify the similarities and differences in epigenetic alterations between the two processes. Understanding the molecular mechanisms underlying epigenetic clocks, especially in somatic stem cell aging, and integrating existing and upcoming epigenetic evidence within genetic damage models of aging will be crucial for better understanding of these interrelated phenomena.
Editorial Material
Biochemistry & Molecular Biology
Flaminia Kaluthantrige Don, Meritxell Huch
Summary: Organoid cultures are promising for both basic and clinical research, with potential breakthroughs, but are currently facing bottlenecks that hinder their full exploitation.
TRENDS IN MOLECULAR MEDICINE
(2021)
Article
Chemistry, Medicinal
Nabanita Nawar, Shazreh Bukhari, Ashley A. Adile, Yujin Suk, Pimyupa Manaswiyoungkul, Krimo Toutah, Olasunkanmi O. Olaoye, Yasir S. Raouf, Abootaleb Sedighi, Harsimran Kaur Garcha, Muhammad Murtaza Hassan, William Gwynne, Johan Israelian, Tudor B. Radu, Mulu Geletu, Ayah Abdeldayem, Justyna M. Gawel, Aaron D. Cabral, Chitra Venugopal, Elvin D. de Araujo, Sheila K. Singh, Patrick T. Gunning
Summary: Through a second-generation structure-activity relationship study, the selective HDAC6 inhibitor NN-390 was designed and evaluated, showing therapeutic potential in the treatment of metastatic Group 3 MB and demonstrating high selectivity and potency in targeting cells in vitro.
JOURNAL OF MEDICINAL CHEMISTRY
(2022)
Article
Clinical Neurology
Radwan Takroni, Nirmeen Zagzoog, Nimita Patel, Amanda Martyniuk, Sheila Singh, Forough Farrokhyar, Arunachala Trivedi, Mazen Alotaibi, Almunder Algird
Summary: This study retrospectively analyzed data from 172 patients with chronic subdural hematoma (CSDH) and compared the effect of two drainage systems on the recurrence rate of CSDH. The results showed no significant difference in the recurrence rate between the use of a pediatric size nasogastric tube and an external ventricular drainage catheter.
JOURNAL OF NEUROLOGICAL SURGERY PART A-CENTRAL EUROPEAN NEUROSURGERY
(2023)
Article
Chemistry, Multidisciplinary
Vincent Huynh, Nazanin Tatari, April Marple, Neil Savage, Dillon McKenna, Chitra Venugopal, Sheila K. Singh, Ryan Wylie
Summary: This study demonstrates the use of displacement affinity release (DAR) to tune the release of a CD133 targeting dual antigen T cell engager (DATE) within a hydrogel. The sustained release enhances T cell mediated killing of cancer spheroids, and the addition of anti-PD1 antibody further enhances the killing effect. The development of a multi-cellular embedded spheroid model with automated microscopy facilitates the identification and translation of delivery vehicles for immunotherapeutics.
JOURNAL OF CONTROLLED RELEASE
(2022)
Article
Immunology
Nazanin Tatari, Xiaoyu Zhang, Shawn C. Chafe, Dillon McKenna, Keith A. Lawson, Minomi Subapanditha, Muhammad Vaseem Shaikh, Mathieu Seyfrid, Neil Savage, Chitra Venugopal, Jason Moffat, Sheila K. Singh
Summary: The expression of CA9 was found to be high in BTICs of GBM and was associated with enhanced self-renewal and proliferation capacity of the stem cells. Researchers developed a novel therapeutic strategy targeting CA9-positive tumor cells and demonstrated its effectiveness in vitro and in vivo.
FRONTIERS IN IMMUNOLOGY
(2022)
Article
Clinical Neurology
Nazanin Tatari, Shahbaz Khan, Julie Livingstone, Kui Zhai, Dillon Mckenna, Vladimir Ignatchenko, Chirayu Chokshi, William D. Gwynne, Manoj Singh, Spencer Revill, Nicholas Mikolajewicz, Chenghao Zhu, Jennifer Chan, Cynthia Hawkins, Jian-Qiang Lu, John P. Provias, Kjetil Ask, Sorana Morrissy, Samuel Brown, Tobias Weiss, Michael Weller, Hong Han, Jeffrey N. Greenspoon, Jason Moffat, Chitra Venugopal, Paul C. Boutros, Sheila K. Singh, Thomas Kislinger
Summary: Glioblastoma (GBM) exhibits cellular and genetic heterogeneity, with a preferential transition from primary disease to recurrent disease towards a mesenchymal state. Immune regulatory/suppressive genes, particularly 2-5-oligoadenylate synthase 2 (OAS2), are identified as important drivers of recurrent GBM.
ACTA NEUROPATHOLOGICA
(2022)
Article
Cell Biology
Maleeha A. Qazi, Sabra K. Salim, Kevin R. Brown, Nicholas Mikolajewicz, Neil Savage, Hong Han, Minomi K. Subapanditha, David Bakhshinyan, Allison Nixon, Parvez Vora, Kimberly Desmond, Chirayu Chokshi, Mohini Singh, Amanda Khoo, Andrew Macklin, Shahbaz Khan, Nazanin Tatari, Neil Winegarden, Laura Richards, Trevor Pugh, Nicholas Bock, Alireza Mansouri, Chitra Venugopal, Thomas Kislinger, Sidhartha Goyal, Jason Moffat, Sheila K. Singh
Summary: Recurrence of solid tumors leads to advanced, treatment-resistant disease with distinct mutational and oncogenic landscape. By analyzing tumor stem cell populations, researchers identified different recurrence patterns and found clonal populations can acquire fitness advantage through therapy. Moreover, they discovered an immunomodulatory signature in the MRD state that has prognostic significance in solid cancers.
Article
Oncology
William D. Gwynne, Yujin Suk, Stefan Custers, Nicholas Mikolajewicz, Jeremy K. Chan, Zsolt Zador, Shawn C. Chafe, Kui Zhai, Laura Escudero, Cunjie Zhang, Olga Zaslaver, Chirayu Chokshi, Muhammad Vaseem Shaikh, David Bakhshinyan, Ian Burns, Iqra Chaudhry, Omri Nachmani, Daniel Mobilio, William T. Maich, Patricia Mero, Kevin R. Brown, Andrew T. Quaile, Chitra Venugopal, Jason Moffat, J. Rafael Montenegro-Burke, Sheila K. Singh
Summary: This research integrated data from genetic screening and metabolomic profiling to identify multiple cancer-selective metabolic vulnerabilities in MYC-driven medulloblastoma, providing potential therapeutic targets. The inhibition of DHODH, an enzyme involved in pyrimidine biosynthesis, leads to metabolic stress response, cell-cycle arrest, and apoptosis in tumor cells. A small-molecule DHODH inhibitor demonstrated strong therapeutic efficacy against patient-derived xenografts in vivo, offering a new treatment option for MYC-driven medulloblastoma.
Article
Clinical Neurology
Leonard H. H. Verhey, Arjuna Maharaj, Nikunj Patel, Branavan Manoranjan, Olufemi Ajani, Adam Fleming, Forough Farrokhyar, Sheila K. K. Singh, Blake Yarascavitch, Pediat Brain Tumor Study Grp
Summary: The purpose of this study was to determine whether intraoperative adjunctive EVD placement in patients with a posterior fossa tumor (PFT) led to improved surgical, radiographic, and clinical outcomes. The results showed that the routine use of EVD as an intraoperative adjunct in clinically stable pediatric patients with posterior fossa tumors and hydrocephalus may not be associated with improved radiological or clinical outcomes.
CHILDS NERVOUS SYSTEM
(2023)
Correction
Multidisciplinary Sciences
Michelle M. Kameda-Smith, Helen Zhu, En-Ching Luo, Yujin Suk, Agata Xella, Brian Yee, Chirayu Chokshi, Sansi Xing, Frederick Tan, Raymond G. Fox, Ashley A. Adile, David Bakhshinyan, Kevin Brown, William D. Gwynne, Minomi Subapanditha, Petar Miletic, Daniel Picard, Ian Burns, Jason Moffat, Kamil Paruch, Adam Fleming, Kristin Hope, John P. Provias, Marc Remke, Yu Lu, Tannishtha Reya, Chitra Venugopal, Jueri Reimand, Robert J. Wechsler-Reya, Gene W. Yeo, Sheila K. Singh
NATURE COMMUNICATIONS
(2023)
Article
Multidisciplinary Sciences
Michelle M. M. Kameda-Smith, Helen Zhu, En-Ching Luo, Yujin Suk, Agata Xella, Brian Yee, Chirayu Chokshi, Sansi Xing, Frederick Tan, Raymond G. G. Fox, Ashley A. A. Adile, David Bakhshinyan, Kevin Brown, William D. D. Gwynne, Minomi Subapanditha, Petar Miletic, Daniel Picard, Ian Burns, Jason Moffat, Kamil Paruch, Adam Fleming, Kristin Hope, John P. P. Provias, Marc Remke, Yu Lu, Tannishtha Reya, Chitra Venugopal, Juri Reimand, Robert J. J. Wechsler-Reya, Gene W. W. Yeo, Sheila K. K. Singh
Summary: The RNA-binding protein Musashi-1 (MSI1) has been found to play an essential role in Group 3 (G3) medulloblastoma (MB), and its inhibition can effectively suppress tumor initiation and prolong survival in G3 MB mouse models and patient-derived xenografts. By identifying MSI1 binding targets in normal neural and G3 MB stem cells and cross-referencing them with large-scale screens at different levels, multiple MYC-associated pathways have been discovered to be selectively targeted by MSI1 in G3 MB, providing valuable resources for context-specific therapeutic strategies.
NATURE COMMUNICATIONS
(2022)
Article
Clinical Neurology
Ieta Shams, Branavan Manoranjan, Rebecca Voth, Malavan Ragulojan, Olufemi Ajani, Blake Yarascavitch, Sheila K. Singh, Adam J. Fleming
Summary: This study retrospectively analyzed 40 cases of progressive juvenile pilocytic astrocytoma (JPA) treated at a single center over an 18-year period (1998-2016). The findings showed that the treatment of progressive JPA is variable and may require numerous surgeries and adjuvant therapies.
JOURNAL OF NEUROSURGICAL SCIENCES
(2023)
Article
Multidisciplinary Sciences
Blessing Iquo Bassey- Archibong, Chirayu Rajendra Chokshi, Nikoo Aghaei, Agata Monika Kieliszek, Nazanin Tatari, Dillon McKenna, Mohini Singh, Minomi Kalpana Subapanditha, Arun Parmar, Daniel Mobilio, Neil Savage, Fred Lam, Tomas Tokar, John Provias, Yu Lu, Shawn Christopher Chafe, Charles Swanton, Robert Edward Hynds, Chitra Venugopal, Sheila Kumari Singh
Summary: Brain metastases (BM) are the most common brain neoplasm in adults, but current therapies have limited efficacy. This study analyzed the transcriptional profile of brain metastasis initiating cells (BMICs) at two distinct stages of the metastatic cascade. The researchers found that premetastatic BMICs exhibited increased expression of HLA-G, which plays a role in promoting brain metastatic lesions. Understanding the molecular landscape of premetastatic BMICs could lead to the development of more effective therapies.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2023)
Article
Oncology
Kaiyun Yang, Yosef Ellenbogen, Amanda Martyniuk, Michel Sourour, Radwan Takroni, Mohamed Somji, Emily Gardiner, Katrina Hui, Devang Odedra, Ramiro Larrazabal, Almunder Algird, Edward Kachur, Kesava Reddy, Naresh Murty, Forough Farrokhyar, Sheila K. Singh
Summary: This study aimed to investigate whether repeat resection for recurrent glioblastoma (GBM) provides a survival benefit and examine the complication profile. The results showed that repeat surgical resection did not significantly increase overall survival (OS) compared to single surgical resection and may lead to more neurological complications.
NEURO-ONCOLOGY ADVANCES
(2022)
Article
Biochemical Research Methods
Yujin Suk, Agata Kieliszek, Daniel Mobilio, Chitra Venugopal, Sheila K. Singh
Summary: This article presents a protocol for the safe and successful derivation and culture of hNSCs from human embryonic brain tissue. The hNSCs can be used as a control in brain cancer research and for downstream applications including RNA-seq and omics studies.
Article
Oncology
Alexandria A. Gonzales, Alexander Mastrolonardo, Kenna Winget, Malavan Ragulojan, Adam J. Fleming, Sheila K. Singh
Summary: Multidisciplinary neuro-oncology clinics allow collaboration between different specialties and training levels, providing a foundation for a reliable clinical research team. However, the lack of literature or guidelines hinders the formation of a research team around these clinics' activities. This perspective paper uses experiences from the McMaster Pediatric Brain Tumour Study Group to offer guidance for those seeking to form a research team based on the collective activities and observations of a multidisciplinary clinic.
FRONTIERS IN ONCOLOGY
(2022)
