Article
Microbiology
Wouter J. C. Feitz, Romy Bouwmeester, Thea J. A. M. van der Velden, Susan Goorden, Christoph Licht, Lambert P. J. W. van den Heuvel, Nicole C. A. J. van de Kar
Summary: This study suggests that inhibiting the synthesis of Gb3 could be a potential therapeutic target for protecting against (further) endothelial damage caused by Stx in the future.
Article
Pediatrics
Daiana S. Sanchez, Lilian K. Fischer Sigel, Alejandro Balestracci, Cristina Ibarra, Maria M. Amaral, Claudia Silberstein
Summary: The study found that EG is effective in preventing the damage caused by Stx2 on human renal epithelial cells by reducing the expression of toxin receptor Gb3, maintaining cell viability and proliferation, and promoting tubulogenesis. This suggests that EG, currently used for Gaucher's disease, could be a potential therapeutic strategy for preventing renal damage caused by Shiga toxin.
PEDIATRIC RESEARCH
(2022)
Review
Immunology
Izabella de Macedo Henrique, Flavia Sacerdoti, Raissa Lozzardo Ferreira, Camila Henrique, Maria Marta Amaral, Roxane Maria Fontes Piazza, Daniela Luz
Summary: This article introduces the structure of Shiga toxins (Stx) and the bacteria that produce them, as well as different types of antibody molecules for Stx neutralization and their potential applications.
FRONTIERS IN CELLULAR AND INFECTION MICROBIOLOGY
(2022)
Article
Medicine, Research & Experimental
Kyung-Soo Lee, Jieun Lee, Pureum Lee, Bong Chan Jeon, Min Yeong Song, Sojung Kwak, Jungwoon Lee, Jun-Seob Kim, Doo-Jin Kim, Ji Hyung Kim, Vernon L. Tesh, Moo-Seung Lee, Sung-Kyun Park
Summary: This study reveals that O-GlcNAcylation is acutely increased upon induction of ER stress by Shiga toxins, and inhibition of this modification effectively suppresses apoptotic and inflammatory responses in susceptible cells. Inhibition of aberrant O-GlcNAcylation improves major disease symptoms and survival rate in mice injected with a lethal dose of Stx, suggesting it as a potential approach to treat Stx-mediated diseases.
EMBO MOLECULAR MEDICINE
(2022)
Article
Pharmacology & Pharmacy
Zeinab Mohseni, Hamid Sedighian, Raheleh Halabian, Jafar Amani, Elham Behzadi, Abbas Ali Imani Fooladi
Summary: The study synthesized a novel antitumor candidate DT389-STXB fusion protein, which exhibited potential cytotoxicity against cancer cells, especially towards GB3-expressing breast cancer cells, demonstrating high affinity and dose-dependent cytotoxicity.
EUROPEAN JOURNAL OF PHARMACOLOGY
(2021)
Article
Immunology
Jie Song, Rui Zhao, Chong Yan, Sushan Luo, Jianying Xi, Peipei Ding, Ling Li, Weiguo Hu, Chongbo Zhao
Summary: This study investigated the potential therapeutic effect of targeted complement inhibitor CRIg/FH in reducing neuromuscular junction (NMJ) injury in rat myasthenia gravis (MG) models. The results showed that CRIg/FH could inhibit complement-dependent cytotoxicity on MG patient-derived cells in vitro. Additionally, administration of CRIg/FH significantly reduced complement-mediated end-plate damage and suppressed the development of MG in rodent models. Moreover, CRIg/FH treatment also reduced the levels of autoantibodies and cytokines while increasing the percentage of Treg cells in the spleen.
FRONTIERS IN IMMUNOLOGY
(2022)
Article
Food Science & Technology
Agnieszka Necel, Sylwia Bloch, Bozena Nejman-Falenczyk, Aleksandra Dydecka, Gracja Topka-Bielecka, Alicja Wegrzyn, Grzegorz Wegrzyn
Summary: This study proposes a validation system for selecting bacteriophages against STEC contamination, and conducts model experiments to explore their potential applications. The results show that bacteriophage vB_Eco4M-7 has a certain bactericidal effect on STEC, but caution should be taken due to the appearance of phage-resistant bacteria.
Article
Biotechnology & Applied Microbiology
Maria Julia Ruiz, Mauro Daniel Garcia, Luis Manuel Medina Canalejo, Alejandra Kruger, Nora Lia Padola, Analia Ines Etcheverria
Summary: This study characterized the Lactiplantibacillus sp. LP5 strain isolated from pork production and identified bacteriocin-like inhibitory substances produced by this strain. LP5 was identified as Lactiplantibacillus plantarum. The bacteriocins produced by LP5 showed inhibitory effects on a Shiga toxin-producing Escherichia coli strain. The presence of genes encoding specific plantaricins was confirmed by PCR and whole genome sequencing.
JOURNAL OF APPLIED MICROBIOLOGY
(2023)
Article
Biochemistry & Molecular Biology
Terunao Iwanaga, Tetsuhiro Chiba, Masato Nakamura, Tatsuya Kaneko, Junjie Ao, Na Qiang, Yaojia Ma, Jiaqi Zhang, Tadayoshi Kogure, Sae Yumita, Takamasa Ishino, Keita Ogawa, Motoyasu Kan, Miyuki Nakagawa, Kisako Fujiwara, Naoto Fujita, Takafumi Sakuma, Hiroaki Kanzaki, Keisuke Koroki, Yuko Kusakabe, Masanori Inoue, Kazufumi Kobayashi, Naoya Kanogawa, Soichiro Kiyono, Takayuki Kondo, Ryo Nakagawa, Sadahisa Ogasawara, Shingo Nakamoto, Ryosuke Muroyama, Jun Kato, Tatsuo Kanda, Hitoshi Maruyama, Naoya Mimura, Takuya Honda, Toshihiko Murayama, Hiroyuki Nakamura, Naoya Kato
Summary: This study demonstrates that miglustat can suppress liver fibrosis by inhibiting the TGF-5/Smad pathway, reducing the expression of extracellular matrix components such as collagen. The research also shows that miglustat can both prevent the occurrence of liver fibrosis and reverse established fibrosis.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2023)
Article
Biochemistry & Molecular Biology
Callum Tromans-Coia, Andrea Sanchi, Giuliana K. Moeller, Gyula Timinszky, Massimo Lopes, Ivan Ahel
Summary: ADP-ribosylation is a crucial cellular process catalyzed by ADP-ribosyltransferases and reversed by ADP-ribosylhydrolases. The study demonstrates TARG1 can reverse thymidine-linked DNA ADP-ribosylation and TARG1-deficient human cells are extremely sensitive to this modification. Additionally, it represents the first detection of reversible ADP-ribosylation on genomic DNA in vivo from human cells, providing insight into the largely unknown facet of ADP-ribosylation.
NUCLEIC ACIDS RESEARCH
(2021)
Article
Cardiac & Cardiovascular Systems
Younes Yassaghi, Sajad Jeddi, Nasibeh Yousefzadeh, Khosrow Kashfi, Asghar Ghasemi
Summary: Long-term nitrate intervention increased the resistance to myocardial ischemia-reperfusion injury (MIRI) in female rats, which was associated with increased heart endothelial nitric oxide synthase (eNOS) expression and circulating progesterone before ischemia and blunting ischemia-induced increased inducible nitric oxide synthase (iNOS) and decreased eNOS after MIRI.
BMC CARDIOVASCULAR DISORDERS
(2023)
Article
Pharmacology & Pharmacy
Mega O. Oyovwi, Onome B. Oghenetega, Emojevwe Victor, Falajiki Y. Faith, Joseph Gregory Uchechukwu
Summary: The purpose of this study was to investigate the potential counteractive effects of quercetin on the reproductive capabilities of rats treated with levetiracetam. The study found that quercetin could alleviate the negative effects of levetiracetam on rat reproductive parameters.
Article
Neurosciences
Mali Cosden, Sarah Jinn, Lihang Yao, Cheryl A. Gretzula, Monika Kandebo, Dawn Toolan, Nathan G. Hatcher, Lei Ma, Wei Lemaire, Gregory C. Adam, Christine Burlein, Christina Minnick, Rose Flick, Marla L. Watt, James Mulhearn, Mark Fraley, Robert E. Drolet, Jacob N. Marcus, Sean M. Smith
Summary: Mutations in the GBA1 gene that encodes the enzyme glucocerebrosidase are the most common genetic risk factor for Parkinson's disease. Reduction of GCase activity due to these mutations leads to accumulation of glycosphingolipids, which may contribute to the pathology of Parkinson's disease by altering lysosomal function. Pharmacological inhibition of GCase and a GBA1 mutation were shown to enhance glycosphingolipid accumulation and insoluble phosphorylated alpha-synuclein, a protein associated with Parkinson's disease.
NEUROBIOLOGY OF DISEASE
(2021)
Article
Microbiology
Xiangning Bai, Flemming Scheutz, Henrik Mellstrom Dahlgren, Ingela Hedenstrom, Cecilia Jernberg
Summary: A novel Stx2m subtype was identified in clinical E. coli strains isolated from Sweden and Denmark, showing functional cytotoxicity and macrolide resistance gene mdf (A). This finding expands the pool of Stx2 subtypes and underscores the clinical significance of emerging STEC variants. This study suggests including Stx2m in epidemiological surveillance and clinical diagnosis of STEC infections.
Article
Cell Biology
Giovana Rampazzo Teixeira, Luiz Gustavo Almeida Chuffa, Leonardo Oliveira Mendes, Allice Santos Cruz Veras, James McCabe, Wagner Jose Favaro, Patricia Fernanda Felipe Pinheiro, Joao Paulo Arruda Amorim, Otavio Augusto Martins, Wilson Mello-Junior, Francisco Eduardo Martinez
Summary: This study investigated the protective effects of strength training against ethanol-induced prostatic metabolism disorders in rats. Strength training reduced alcohol consumption, increased glucose and serum lipid levels, and decreased hormonal levels. Protein expression analysis showed significant differences in hormone receptors and cell regulation between ethanol-exposed and strength training groups, suggesting strength training as a potential therapeutic strategy for ethanol-induced prostate injuries.
JOURNAL OF CELLULAR PHYSIOLOGY
(2021)
Article
Medicine, Research & Experimental
Patricia Arza, Vanina Netti, Francisco Perosi, Gustavo Cernadas, Federico Ochoa, Natalia Magnani, Pablo Evelson, Elsa Zotta, Andrea Fellet, Ana M. Balaszczuk
BIOMEDICINE & PHARMACOTHERAPY
(2015)
Correction
Cell Biology
Johanna G. Miquet, Thomas Freund, Carolina S. Martinez, Lorena Gonzalez, Maria E. Diaz, Giannina P. Micucci, Elsa Zotta, Ravneet K. Boparai, Andrzej Bartke, Daniel Turyn, Ana I. Sotelo
Article
Microbiology
Adriana Albanese, Elizabeth Gerhardt, Hugo Garcia, Natalia Amigo, Angel Cataldi, Elsa Zotta, Cristina Ibarra
INTERNATIONAL JOURNAL OF MEDICAL MICROBIOLOGY
(2015)
Article
Pharmacology & Pharmacy
Silvina Chimeno Zoth, Juan Manuel Carballeda, Maria Jose Gravisaco, Maria Soledad Lucero, Matias Richetta, Evangelina Gomez, Analia Berinstein
ANTIVIRAL RESEARCH
(2016)
Article
Medicine, Research & Experimental
Vanina A. Netti, Agustina N. Iovane, Mariana C. Vatrella, Elsa Zotta, Andrea L. Fellet, Ana M. Balaszczuk
BIOMEDICINE & PHARMACOTHERAPY
(2016)
Article
Pharmacology & Pharmacy
Maria Pilar Mejias, Romina Jimena Fernandez-Brando, Maria Victoria Ramos, Maria Jimena Abrey-Recalde, Elsa Zotta, Roberto Meiss, Marina Sandra Palermo
CURRENT PHARMACEUTICAL DESIGN
(2016)
Article
Urology & Nephrology
Hernan Trimarchi, Romina Canzonieri, Amalia Schiel, Juan Politei, Anibal Stern, Jos Andrews, Matias Paulero, Tatiana Rengel, Alicia Araoz, Mariano Forrester, Fernando Lombi, Vanesa Pomeranz, Romina Iriarte, Pablo Young, Alexis Muryan, Elsa Zotta
JOURNAL OF NEPHROLOGY
(2016)
Correction
Urology & Nephrology
Hernan Trimarchi, Romina Canzonieri, Amalia Schiel, Juan Politei, Anibal Stern, Jose Andrews, Matias Paulero, Tatiana Rengel, Alicia Araoz, Mariano Forrester, Fernando Lombi, Vanesa Pomeranz, Romina Iriarte, Pablo Young, Alexis Muryan, Elsa Zotta
JOURNAL OF NEPHROLOGY
(2016)
Article
Medicine, Research & Experimental
H. Trimarchi, R. Canzonieri, A. Schiel, C. Costales-Collaguazo, J. Politei, A. Stern, M. Paulero, T. Rengel, J. Andrews, M. Forrester, M. Lombi, V. Pomeranz, R. Iriarte, A. Muryan, E. Zotta, M. D. Sanchez-Nino, A. Ortiz
JOURNAL OF TRANSLATIONAL MEDICINE
(2016)
Article
Pharmacology & Pharmacy
E. Abril Seyahian, Gisela Oltra, Federico Ochoa, Santiago Melendi, Ricardo Hermes, James C. Paton, Adrienne W. Paton, Nestor Lago, Mauricio Castro Parodi, Alicia Damiano, Cristina Ibarra, Elsa Zotta
Article
Immunology
Matias Richetta, Evangelina Gomez, Maria Soledad Lucero, Silvina Chimeno Zoth, Maria Jose Gravisaco, Gabriela Calamante, Analia Berinstein
Article
Endocrinology & Metabolism
Jimena Soutelo, Yanina Alejandra Samaniego, Elsa Zotta, Maria Cecilia Fornari, Carlos Reyes Toso, Osvaldo Juan Ponzo
INTERNATIONAL JOURNAL OF ENDOCRINOLOGY
(2017)
Article
Nutrition & Dietetics
Vanina A. Netti, Agustina N. Iovane, Mariana C. Vatrella, Natalia D. Magnani, Pablo A. Evelson, Elsa Zotta, Andrea L. Fellet, Ana Maria Balaszczuk
EUROPEAN JOURNAL OF NUTRITION
(2016)
Article
Immunology
Maria S. Lucero, Madras Richetta, Silvina Chimeno Zoth, Juan Jaton, Silvina Pint, Zulma Canet, Analia Berinstein, Evangelina Gomez
Article
Immunology
Alan M. Bernal, Romina Jimena Fernandez-Brando, Andrea Cecilia Bruballa, Gabriela A. Fiorentino, Gonzalo Ezequiel Pineda, Elsa Zotta, Monica Vermeulen, Maria Victoria Ramos, Martin Rumbo, Marina Sandra Palermo
Summary: Despite similar susceptibility to Shiga toxin and comparable intestinal bacterial burden between C57BL/6 and BALB/c mice, C57BL/6 mice exhibited more severe intestinal damage and impaired renal function leading to increased mortality, while the higher survival rate in BALB/c mice was associated with an early specific antibody response as part of a tolerance mechanism.
INFECTION AND IMMUNITY
(2021)