Article
Genetics & Heredity
M. M. De Souza, D. A. Koltes, H. Beiki, M. A. Sales, T. Tsai, C. V. Maxwell, J. Zhao, J. E. Koltes
Summary: Exposure to less hygienic conditions during early childhood has been linked to immune system development. This study investigated the impact of early-life topsoil exposure on RNA expression in piglets' blood cells and identified changes in genes related to T-cell activation and antigen-presenting cells.
FRONTIERS IN GENETICS
(2022)
Article
Materials Science, Multidisciplinary
Byeongyeon Kim, In-Ho Seo, Gwang Myeong Seo, Woo-Joong Kim, Eui-Cheol Shin, Sungyoung Choi
Summary: The study introduces a blood divider (B-DV) as a centrifuge tube insert to prevent RBC contamination during PBMC harvest, simplifying the separation process and enabling rapid, easy PBMC isolation.
ADVANCED MATERIALS TECHNOLOGIES
(2022)
Article
Pharmacology & Pharmacy
Vera Vorstandlechner, Dragan Copic, Katharina Klas, Martin Direder, Bahar Golabi, Christine Radtke, Hendrik J. Ankersmit, Michael Mildner
Summary: This study demonstrates the anti-fibrotic effect of PBMCsec on cutaneous scars in both mouse and human experimental settings. PBMCsec regulates the expression of fibrosis-related genes, attenuates myofibroblast differentiation, and inhibits the breakdown of elastic fibers. These findings suggest that PBMCsec could be a novel therapeutic option for treating skin scarring.
Article
Physiology
Sara Magnani, Gabriele Mulliri, Silvana Roberto, Fabio Sechi, Giovanna Ghiani, Gianmarco Sainas, Giorgio Nughedu, Romina Vargiu, Pier Paolo Bassareo, Antonio Crisafulli
Summary: The study showed that performing mild exercise in acute hypoxia does not impair myocardial systolic or diastolic functions, but rather enhances cardiac functions. There were no significant effects on left ventricular volumes, while exercise promoted cardiac systolic and early diastolic functions.
FRONTIERS IN PHYSIOLOGY
(2021)
Article
Biochemistry & Molecular Biology
Belay Tessema, Janine Riemer, Ulrich Sack, Brigitte Koenig
Summary: The results of CSA parameters measured in PBMCs are influenced by the PBMC isolation method, age, seasonal variation, and gender. Researchers and physicians should properly interpret the results of CSA parameters in PBMCs by considering these factors. Separate CSA evaluation standards should be used based on the isolation method, age, gender, and season-dependent factors.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Oncology
Annette Bellar, Nicole Welch, Jaividhya Dasarathy, Amy Attaway, Ryan Musich, Avinash Kumar, Jinendiran Sekar, Saurabh Mishra, Yana Sandlers, David Streem, Laura E. Nagy, Srinivasan Dasarathy
Summary: Patients with acute alcohol-associated hepatitis (AH) have impaired immune function and abnormal mitochondrial function, leading to cellular senescence. Understanding these cellular changes is crucial for the understanding of the pathogenesis of AH.
CLINICAL AND TRANSLATIONAL MEDICINE
(2023)
Article
Medicine, General & Internal
Keiichiro Yoshioka, Hironori Sato, Takeshi Kawasaki, Daisuke Ishii, Takuro Imamoto, Mitsuhiro Abe, Yoshinori Hasegawa, Osamu Ohara, Koichiro Tatsumi, Takuji Suzuki
Summary: RNA sequencing analysis revealed differential gene expression patterns in peripheral blood mononuclear cells (PBMCs) between patients with pulmonary sarcoidosis and healthy controls, with 270 differentially expressed genes involved in various biological processes related to the pathobiology of sarcoidosis. Enrichment analysis of gene ontology and KEGG pathways indicated the potential involvement of specific signaling pathways in sarcoidosis pathogenesis, providing insight into potential therapeutic targets for the disease.
FRONTIERS IN MEDICINE
(2022)
Review
Physiology
Isabela Roque Marcal, Karla Fabiana Goessler, Roselien Buys, Juliano Casonatto, Emmanuel Gomes Ciolac, Veronique A. Cornelissen
Summary: Post-exercise hypotension (PEH) is an important tool for managing hypertension, with high-intensity interval exercise (HIIE) showing a greater reduction in blood pressure during daytime ambulatory monitoring compared to moderate-intensity continuous exercise (MICE). Further studies are needed to confirm HIIE's superiority as a safe intervention for lowering blood pressure, particularly in older individuals with hypertension.
FRONTIERS IN PHYSIOLOGY
(2021)
Article
Chemistry, Multidisciplinary
Pat R. Vehrs, Chase Blazzard, Hannah C. Hart, Nicole Kasper, Ryan Lacey, Daniela Lopez, Shay Richards, Dennis L. Eggett
Summary: This study measured arterial occlusion pressure (AOP) in the dominant and non-dominant legs of males and females using different cuff inflation protocols. The results showed no significant differences in AOP within or between the legs, or between sexes. AOP measurements were highly reliable, and the choice of cuff inflation protocol was a matter of personal preference. It is important to measure AOP in both limbs to ensure safe and effective use of blood flow restriction (BFR) during exercise.
APPLIED SCIENCES-BASEL
(2023)
Article
Biochemistry & Molecular Biology
Hanwen Chen, Peiwei Li, Jiamin Chen, Yufang Wang, Qiao Yu, Yihua Wu, Yan Chen, Jianting Cai
Summary: In this study, PBMC miR-582-5p was identified as a potential diagnostic biomarker for Crohn's disease, while miR-96-5p may serve as a novel and valuable biomarker for monitoring disease activity.
Review
Medicine, Research & Experimental
Pamela Carvalho Da Rosa, Judit Borras Bertomeu, Luis Fernando Freire Royes, Raul Osiecki
Summary: Peripheral blood mononuclear cells (PBMCs) are specialized immune cells produced in the bone marrow that protect the body from pathogens and can serve as a sentinel tissue source for monitoring physiological changes. Physical exercise has been shown to influence the metabolic state and immune system through its interactions with PBMCs. However, the specific metabolic processes and biomarkers related to exercise-induced changes in PBMCs are not fully understood. This review aims to summarize the current literature on the inflammatory and oxidative stress responses of PBMCs to physical exercise and highlights their potential for assessing exercise-induced metabolic adaptations.
Article
Cell Biology
Abhirup Bagchi, Aneesha Nath, Vasanth Thamodaran, Smitha Ijee, Dhavapriya Palani, Vignesh Rajendiran, Vigneshwaran Venkatesan, Phaneendra Datari, Aswin Anand Pai, Nancy Beryl Janet, Poonkuzhali Balasubramanian, Yukio Nakamura, Alok Srivastava, Kumarasamypet Murugesan Mohankumar, Saravanabhavan Thangavel, Shaji R. Velayudhan
Summary: This study established a protocol for culturing early-stage EPCs from peripheral blood and immortalizing them by expressing HEE genes, providing a new cellular model for studying the molecular mechanisms of human erythropoiesis. The two iEPCs generated showed stable doubling times and erythroid progenitor properties, suitable for studying therapeutic targets and mechanisms of red cell diseases.
Article
Cell & Tissue Engineering
Chang-Han Chuang, Chi-Chung Kuo, Yueh-Feng Chiang, Pei-Yuan Lee, Fu-Hui Wang, Chia-Ying Hsieh, Ching- Shen, Yu-Hsuan Chung, Kuan-Der Lee, Shih-Fang Wu, Hong-Lin Su, Chih-Lung Lin
Summary: This study examines the effect of peripheral blood-derived mononuclear cells (PBMNCs) on patients with knee osteoarthritis (KOA). The study found that intra-articular injection of autologous PBMNCs significantly attenuated knee pain and motor dysfunction in patients, and the therapeutic effect could be stably maintained for up to 24 months.
CELL TRANSPLANTATION
(2023)
Article
Oncology
Jacobo Rogado, Fernando Pozo, Kevin Troule, Jose Miguel Sanchez-Torres, Nuria Romero-Laorden, Rebeca Mondejar, Olga Donnay, Anabel Ballesteros, Vilma Pacheco-Barcia, Javier Aspa, Fatima Al-Shahrour, Arantzazu Alfranca, Ramon Colomer
Summary: Peripheral blood mononuclear cells (PBMCs) have been explored as potential biomarkers to predict the efficacy of anti-PD-1 immunotherapy in advanced non-small cell lung cancer (NSCLC) patients. High baseline levels of circulating T cell subpopulations related to tissue lymphocyte recruitment were found to be associated with poorer outcomes in immunotherapy-treated advanced NSCLC patients.
Article
Multidisciplinary Sciences
Lukasz Antoniewicz, Mikael Kabele, Ulf Nilsson, Jamshid Pourazar, Gregory Rankin, Jenny A. Bosson, Magnus Lundback
Summary: This study evaluates the effects of chronic snus use on vascular function and finds that chronic snus users have increased arterial stiffness and reduced endothelial vasodilatory function compared to non-users. These findings suggest that long-term use of snus is correlated with an increased risk of cardiovascular disease development.