Review
Cardiac & Cardiovascular Systems
Yidong Wang, Yuan Fang, Pengfei Lu, Bingruo Wu, Bin Zhou
Summary: NOTCH signaling regulates multiple biological processes involved in tissue morphogenesis and homeostasis. Mutations in NOTCH1 can cause congenital bicuspid aortic valve and calcific aortic valve disease, with genetic variants affecting other genes in the pathway also contributing to these diseases. The role of NOTCH signaling in the postnatal homeostasis of the aortic valve is crucial, as animal studies have provided detailed insights into the developmental etiology of congenital aortic valve defects.
FRONTIERS IN CARDIOVASCULAR MEDICINE
(2021)
Article
Multidisciplinary Sciences
Abhijeet P. Deshmukh, Suhas V. Vasaikar, Katarzyna Tomczak, Shubham Tripathi, Petra den Hollander, Emre Arslan, Priyanka Chakraborty, Rama Soundararajan, Mohit Kumar Jolly, Kunal Rai, Herbert Levine, Sendurai A. Mani
Summary: This study identified the complexity of the EMT process by analyzing gene expression profiles during EMT progression. It demonstrated that NOTCH signaling pathway drives TGF-beta-induced EMT, and gene signatures corresponding to intermediate hybrid EMT state are associated with poor patient outcome.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2021)
Article
Oncology
Zhihui Gao, Xiaojian Ni, Bohao Zheng, Wentao Sun, Wenze Wan, Han Liu, Xiaoling Ni, Tao Suo, Na Li, Houbao Liu, Sheng Shen
Summary: This study investigated the biological functions and molecular mechanisms of Biliverdin reductase B (BLVRB) in human cholangiocarcinoma (CCA). The results showed that BLVRB depletion accelerated epithelial-mesenchymal transition (EMT), cell migration, and invasion, while BLVRB overexpression reduced EMT and cell migration and invasion in CCA. Furthermore, BLVRB suppression activated Notch signaling, which enhanced EMT and increased cell migration and invasion in CCA.
Article
Cell Biology
Victor Olariu, Mary A. Yui, Pawel Krupinski, Wen Zhou, Julia Deichmann, Emil Andersson, Ellen Rothenberg, Carsten Peterson
Summary: This study presents a three-level dynamic model to explain the commitment dynamics of thymic cell development, including a core gene regulatory network architecture, stochastically controlled chromatin-state gate, and single-cell proliferation model.
Article
Multidisciplinary Sciences
Jiexiang Zhao, Ping Lu, Cong Wan, Yaping Huang, Manman Cui, Xinyan Yang, Yuqiong Hu, Yi Zheng, Ji Dong, Mei Wang, Shu Zhang, Zhaoting Liu, Shuhui Bian, Xiaoman Wang, Rui Wang, Shaofang Ren, Dazhuang Wang, Zhaokai Yao, Gang Chang, Fuchou Tang, Xiao-Yang Zhao
Summary: Through single-cell sequencing, the study investigates the transcriptome landscapes of mouse male germ cells throughout development and identifies critical regulators for prenatal cell-fate determination.
NATURE COMMUNICATIONS
(2021)
Article
Biochemistry & Molecular Biology
Francesca Giordano, Maria D'Amico, Francesca Ida Montalto, Rocco Malivindi, Adele Chimento, Francesca Luisa Conforti, Vincenzo Pezzi, Maria Luisa Panno, Sebastiano Ando, Francesca De Amicis
Summary: Glioblastoma multiforme (GBM) is an aggressive cancer type with poor outcomes, and enrichment of GBM stem cells (GSCs) is believed to be the cause of its recurrence and chemoresistance. In this study, low-dose treatment of γ-secretase inhibitor RO4929097 (GSI) and resveratrol (RSV) reversed the mesenchymal phenotype of GBM cells to an epithelial-like phenotype, affecting invasion and stemness. The mechanism involves cyclin D1, cyclin-dependent kinase (CDK4), and paxillin (Pxn) phosphorylation, leading to reduced Pxn interaction with vinculin (Vcl) and altered cell migration. The study suggests that targeting Cdk4 with Notch inhibitors and RSV could be a potential therapeutic strategy for aggressive brain tumors.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Anatomy & Morphology
Veronika Olejnickova, Peter Uriel Hamor, Jiri Janacek, Martin Bartos, Eva Zabrodska, Barbora Sankova, Alena Kvasilova, Hana Kolesova, David Sedmera
Summary: This study reveals the essential role of trabeculae in intraventricular impulse spreading and conduction patterning in the early endothermic heart. Lack of trabeculae leads to a failure in conduction parameters differentiation and impacts the cardiac pumping function.
DEVELOPMENTAL DYNAMICS
(2022)
Article
Biochemistry & Molecular Biology
Luigi Borzacchiello, Roberta Veglia Tranchese, Roberta Grillo, Roberta Arpino, Laura Mosca, Giovanna Cacciapuoti, Marina Porcelli
Summary: Recent research has found a correlation between dysregulated Notch and tumor metastasis in colorectal cancer (CRC). The study also showed that S-adenosyl-L-methionine (AdoMet) could act as a potential antimetastatic agent by modulating the expression of miRNA-34a/-34c/-449a and inhibiting the migration of CRC cells.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Multidisciplinary Sciences
Jeremy Lotto, Rebecca Cullum, Sibyl Drissler, Martin Arostegui, Victoria C. Garside, Bettina M. Fuglerud, Makenna Clement-Ranney, Avinash Thakur, T. Michael Underhill, Pamela A. Hoodless
Summary: This study used single-cell transcriptomics to analyze the complexity and mechanisms of epithelial-to-mesenchymal transitions (EMTs) and heart valve development in the endocardium and epicardium. The researchers identified the single-cell transcriptomes of murine hearts from embryonic day (E)7.75 to E12.5 and described the processes of mesenchymal and endocardial bifurcation, epicardial transdifferentiation, and activation of epithelial-mesenchymal plasticity. They also found that SOX9 regulates endothelial and mesenchymal cell fate decisions in valve development.
NATURE COMMUNICATIONS
(2023)
Article
Medicine, Research & Experimental
Ludovica Cotellessa, Federica Marelli, Paolo Duminuco, Michela Adamo, Georgios E. . Papadakis, Lucia Bartoloni, Naoko Sato, Mariarosaria Lang-Muritano, Amineh Troendle, Waljit S. . Dhillo, Annamaria Morelli, Giulia Guarnieri, Nelly Pitteloud, Luca Persani, Marco Bonomi, Paolo Giacobini, Valeria Vezzoli
Summary: Fertility in vertebrate species is controlled by GnRH neurons, which arise outside the central nervous system and migrate into the forebrain during development. Genetic disorders associated with infertility, such as CHH and Kallmann syndrome, are characterized by defects in GnRH neuron migration. The study found that Jag1/Notch signaling is involved in the development of GnRH neurons, and JAG1 insufficiency may contribute to the pathogenesis of CHH in humans.
Article
Multidisciplinary Sciences
Nazanin Ebrahimi, Mahyar Osanlouy, Chris P. Bradley, M. Fabiana Kubke, Dane A. Gerneke, Peter J. Hunter
Summary: This study developed a workflow using multi-scale and multi-disciplinary approaches to analyze the C-looping phase of chick heart development. The researchers provided the first 3D datasets of the C-looping heart, including heart images and segmented myocardial cell data. The results revealed differential spatiotemporal growth patterns at both cellular and tissue levels and identified potential candidates for the mechanism of C-looping.
Article
Cell Biology
Pengxiang Wang, Jun Su, Junpeng Wang, Yilin Xie, Wei Chen, Jinhai Zhong, Yuan Wang
Summary: This study demonstrates that the regulator NRF1 plays critical roles in post-migrating primordial germ cell (PGC) development. NRF1 protein level gradually increases in post-migrating PGCs and directly regulates key molecules involved in PGC formation, proliferation, cell cycle, and mitochondrial metabolism. The study also suggests that NRF1 promotes the derivation of PGCs from pluripotent stem cells.
CELL PROLIFERATION
(2023)
Article
Multidisciplinary Sciences
Mikiko Inaki, Smitha Vishnu, Kenji Matsuno
Summary: Delaminating cells undergo complex, precisely regulated changes in cell-cell adhesion, motility, polarity, invasiveness, and other cellular properties. In the study of epithelial delamination in Drosophila ovary border cells, it was found that invasiveness and motility are cooperatively required for delamination and regulated independently. Additionally, the acquisition of invasiveness does not depend on the prerequisite of motility.
SCIENTIFIC REPORTS
(2022)
Article
Cell Biology
Edroaldo Lummertz da Rocha, Caroline Kubaczka, Wade W. Sugden, Mohamad Ali Najia, Ran Jing, Arianna Markel, Zachary C. LeBlanc, Rafael dos Santos Peixoto, Marcelo Falchetti, James J. Collins, Trista E. North, George Q. Daley
Summary: The study presents an algorithm called CellComm that analyzes cell-cell communication, predicts signaling and regulatory networks, and identifies regulators of hematopoietic development. Using zebrafish, mouse, and human models, the study confirms the accuracy of CellComm in identifying key regulators of hematopoiesis.
NATURE CELL BIOLOGY
(2022)
Article
Cell Biology
David van Bruggen, Fabio Pohl, Christoffer Mattsson Langseth, Petra Kukanja, Hower Lee, Alejandro Mossi Albiach, Mukund Kabbe, Mandy Meijer, Sten Linnarsson, Markus M. Hilscher, Mats Nilsson, Erik Sundstrom, Goncalo Castelo-Branco
Summary: The study identified the emergence of the first wave of oligodendrocyte lineage cells in the human forebrain as early as the 8th week of gestation, indicating evolutionary conservation of this process between mice and humans. Through analyzing the spatial expression of 50 key genes, regions in the human ventral fetal forebrain where oligodendrogenesis first occurs were identified.
DEVELOPMENTAL CELL
(2022)
Article
Anatomy & Morphology
Laura Andres-Delgado, Maria Galardi-Castilla, Juliane Muench, Marina Peralta, Alexander Ernst, Juan Manuel Gonzalez-Rosa, Federico Tessadori, Luis Santamaria, Jeroen Bakkers, Julien Vermot, Jose Luis de la Pompa, Nadia Mercader
DEVELOPMENTAL DYNAMICS
(2020)
Article
Multidisciplinary Sciences
Dimitrios Grivas, Alvaro Gonzalez-Rajal, Carlos Guerrero Rodriguez, Ricardo Garcia, Jose Luis de la Pompa
SCIENTIFIC REPORTS
(2020)
Article
Cardiac & Cardiovascular Systems
Jose A. de la Chica, Sandra Gomez-Talavera, Jose M. Garcia-Ruiz, Ines Garcia-Lunar, Belen Oliva, Juan M. Fernandez-Alvira, Beatriz Lopez-Melgar, Javier Sanchez-Gonzalez, Jose L. de la Pompa, Jose M. Mendiguren, Vicente Martinez de Vega, Antonio Fernandez-Ortiz, Javier Sanz, Leticia Fernandez-Friera, Borja Ibanez, Valentin Fuster
JOURNAL OF THE AMERICAN COLLEGE OF CARDIOLOGY
(2020)
Article
Cell Biology
Donal MacGrogan, Beatriz Martinez-Poveda, Jean-Pierre Desvignes, Leticia Fernandez-Friera, Manuel Jose Gomez, Eduardo Gil Vilarino, Sergio Callejas Alejano, Pablo Garcia-Pavia, Jorge Solis, Joaquin Lucena, David Salgado, Gwenaelle Collod-Beroud, Emilie Faure, Alexis Theron, Julia Torrents, Jean-Francois Avierinos, Lorena Montes, Ana Dopazo, Valentin Fuster, Borja Ibanez, Fatima Sanchez-Cabo, Stephane Zaffran, Jose Luis de la Pompa
PHYSIOLOGICAL GENOMICS
(2020)
Article
Cell Biology
Dimitrios Grivas, Alvaro Gonzalez-Rajal, Jose Luis de la Pompa
Summary: Unlike mammalian hearts, adult zebrafish hearts can regenerate after injury. Mdka plays a critical role in fibrosis regulation during zebrafish heart regeneration, with loss of mdka resulting in decreased endothelial cell proliferation and an arrest in heart regeneration characterized by a collagenous scar. Increased collagen transcription and intense TGF beta signaling activity were also observed in mdka knockout zebrafish strains.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2021)
Editorial Material
Cardiac & Cardiovascular Systems
Donal MacGrogan, Jose Luis de la Pompa
CIRCULATION RESEARCH
(2021)
Article
Cell Biology
Belen Prados, Raquel del Toro, Donal MacGrogan, Paula Gomez-Apinaniz, Tania Papoutsi, Pura Munoz-Canoves, Simon Mendez-Ferrer, Jose Luis de la Pompa
Summary: Bmp2 signaling is crucial for vascular/hematopoietic lineages, with ectopic expression leading to soft tissue bone formation and HO resembling FOP in mice. Transgenic mice with aberrant Bmp2 activity recapitulate HO pathophysiology, providing a model for investigating potential therapies for disorders related to abnormal bone formation.
CELL DEATH & DISEASE
(2021)
Article
Multidisciplinary Sciences
Rebeca Torregrosa-Carrion, Rebeca Pineiro-Sabaris, Marcos Siguero-Alvarez, Joaquim Grego-Bessa, Luis Luna-Zurita, Vitor Samuel Fernandes, Donal MacGrogan, Didier Y. R. Stainier, Jose Luis de la Pompa
Summary: Mutations in the GPR126/ADGRG6 gene lead to human diseases, particularly affecting peripheral nervous system myelination. Research on mice and zebrafish models demonstrates the impact of Gpr126 deficiency on development, with a proposed placenta-heart axis contributing to heart abnormalities in Gpr126 mutants.
Article
Multidisciplinary Sciences
Tamara Gonzalez-Costa, Jose Luis de la Pompa, Joaquim Grego-Bessa
Summary: The cellular and molecular mechanisms of mammalian heart development are crucial for understanding and addressing human congenital heart disease. This study focuses on the early stages of cardiac valve development, specifically looking at the role of the planar cell polarity pathway in endocardial cell behavior. A novel experimental approach is introduced to visualize the planar field of endocardial cells in valvulogenic regions, allowing for the study of intercellular communication and morphological changes during valve development.
JOVE-JOURNAL OF VISUALIZED EXPERIMENTS
(2022)
Article
Cardiac & Cardiovascular Systems
Marcos Siguero-Alvarez, Alejandro Salguero-Jimenez, Joaquim Grego-Bessa, Jorge de la Barrera, Donal MacGrogan, Belen Prados, Fernando Sanchez-Saez, Rebeca Pineiro-Sabaris, Natalia Felipe-Medina, Carlos Torroja, Manuel Jose Gomez, Maria Sabater-Molina, Ruben Escriba, Ivonne Richaud-Patin, Olalla Iglesias-Garcia, Mauro Sbroggio, Sergio Callejas, Declan P. O'Regan, Kathryn A. McGurk, Ana Dopazo, Giovanna Giovinazzo, Borja Ibanez, Lorenzo Monserrat, Jose Maria Perez-Pomares, Fatima Sanchez-Cabo, Alberto M. Pendas, Angel Raya, Juan R. Gimeno-Blanes, Jose Luis de la Pompa
Summary: The study reveals that MIB1-NOTCH variants play a crucial role in the development of both LVNC and bicuspid aortic valve, along with other genetic modifiers.
Article
Cardiac & Cardiovascular Systems
Andres Pun-Garcia, Agustin Clemente-Moragon, Rocio Villena-Gutierrez, Monica Gomez, David Sanz-Rosa, Anabel Diaz-Guerra, Belen Prados, Juan Pablo Medina, Fermi Monto, Maria Dolores Ivorra, Cristina Marquez-Lopez, Alessandro Cannavo, Juan A. Bernal, Walter J. Koch, Valentin Fuster, Jose Luis de la Pompa, Eduardo Oliver, Borja Ibanez
Summary: This study suggests that beta 3AR overexpression may be beneficial in treating HF induced by AS, indicating a potential therapeutic strategy.
BASIC RESEARCH IN CARDIOLOGY
(2022)
Editorial Material
Cardiac & Cardiovascular Systems
Jose Luis de la Pompa
Article
Cardiac & Cardiovascular Systems
Joaquim Grego-Bessa, Paula Gomez-Apinaniz, Belen Prados, Manuel Jose Gomez, Donal MacGrogan, Jose Luis de la Pompa
Summary: This study investigated the functions and downstream mechanisms of Nrg1 signaling during ventricular chamber development. The Nrg1-ErbB2/ErbB4-Erk axis was found to be a crucial regulator of cardiomyocyte cell cycle progression and migration.
CIRCULATION RESEARCH
(2023)
Article
Cardiac & Cardiovascular Systems
Idit Tessler, Juliette Albuisson, Rebeca Pineiro-Sabaris, Aline Verstraeten, Hatem Elif Kamber Kaya, Marcos Siguero-Alvarez, Guillaume Goudot, Donal MacGrogan, Ilse Luyckx, Shoshana Shpitzen, Galina Levin, Guy Kelman, Noga Reshef, Hugo Mananet, Jake Holdcraft, Jochen D. Muehlschlegel, Gina M. Peloso, Olya Oppenheim, Charles Cheng, Jean-Michael Mazzella, Gregor Andelfinger, Seema Mital, Per Eriksson, Clarisse Billon, Mahyar Heydarpour, Harry C. Dietz, Xavier Jeunemaitre, Eran Leitersdorf, David Sprinzak, Stephen C. Blacklow, Simon C. Body, Shai Carmi, Bart Loeys, Jose Luis de la Pompa, Dan Gilon, Emmanuel Messas, Ronen Durst
Summary: This study identified the MIB1 gene as associated with nsBAV through genetic association analysis. The gene plays a crucial role in NOTCH signal activation during heart development, highlighting its potential for future diagnostic and therapeutic intervention.
Article
Biochemistry & Molecular Biology
P. Palmquist-Gomes, A. Ruiz-Villalba, J. A. Guadix, J. P. Romero, B. Bessieres, D. MacGrogan, L. Conejo, A. Ortiz, B. Picazo, L. Houyel, D. Gomez-Cabrero, S. M. Meilhac, J. L. de la Pompa, J. M. Perez-Pomares
Summary: Coronary Artery Fistulae (CAFs) are cardiac congenital anomalies characterized by abnormal communication between a coronary artery and a heart chamber or vessel. The cause of CAFs is still unknown. Using mouse and avian embryos as models, researchers have found that disruptions in epicardial development and ventricular wall growth lead to the formation of abnormal coronary-like structures in direct connection with the ventricular lumen. These findings provide insights into the early diagnosis and molecular mechanisms of CAFs.
EXPERIMENTAL AND MOLECULAR MEDICINE
(2023)