Article
Urology & Nephrology
Nahid Punjani, Ryan Flannigan, Caroline Kang, Francesca Khani, Peter N. Schlegel
Summary: The study aimed to determine the association between testicular histopathological heterogeneity and sperm retrieval rates (SRRs) in men with nonobstructive azoospermia (NOA) undergoing microdissection testicular sperm extraction (mTESE). The findings revealed that increasing testicular histopathological heterogeneity is correlated with higher SRRs in men with NOA, driven by the identification of focal areas of spermatogenesis. These results highlight the importance of histology in potentially predicting the chance of sperm retrieval in future mTESE procedures.
JOURNAL OF UROLOGY
(2021)
Article
Urology & Nephrology
Christian Fuglesang S. Jensen, Dana A. Ohl, Mikkel Fode, Niels Jorgensen, Aleksander Giwercman, Niels Henrik Bruun, Angel Elenkov, Anna Klajnbard, Claus Y. Andersen, Lise Aksglaede, Marie Louise Grondahl, Mette C. Bekker, Jens Sonksen
Summary: This study compared mTESE and multiple needle-pass TESA in men with NOA, and found that mTESE had a higher sperm retrieval rate than multiple needle-pass TESA.
Article
Obstetrics & Gynecology
Ahmad Aboukhshaba, Nahid Punjani, Sofia Doukakis, Nikica Zaninovic, Gianpiero Palermo, Peter N. Schlegel
Summary: The study aimed to evaluate the impact of sperm characteristics on intracytoplasmic sperm injection (ICSI) outcomes in men with nonobstructive azoospermia (NOA) following microdissection testicular sperm extraction (mTESE). The results showed that specific sperm defects did not completely preclude the chance of clinical pregnancy. This study is the first comprehensive evaluation of the importance of these characteristics in ICSI outcomes.
FERTILITY AND STERILITY
(2022)
Review
Medicine, General & Internal
Giovanni M. Colpi, Ettore Caroppo
Summary: Microdissection testicular sperm extraction (mTESE) is considered the gold-standard surgical technique for retrieving testicular sperm in patients with non-obstructive azoospermia (NOA). A skilled surgeon is required for mTESE, and the learning curve may impact the sperm retrieval rate. This review provides a detailed description of the surgical procedure, accompanied by illustrations, and offers advice on pre-surgical and post-surgical patient management.
JOURNAL OF CLINICAL MEDICINE
(2021)
Article
Medicine, General & Internal
James Wren, Matthew Hudnall, Minh Pham, Anne L. Darves-Bornoz, Joshua A. Halpern, Nelson E. Bennett, Robert E. Brannigan, Matthias D. Hofer
Summary: This study aimed to compare the rates of successful sperm retrieval in patients with a prior history of cryptozoospermia and patients with non-obstructive azoospermia (NOA) using microdissection testicular sperm extraction (mTESE). The results showed that patients with cryptozoospermia had a significantly higher chance of successful mTESE compared to patients with NOA. Factors such as pathology and hormone levels were associated with the success of mTESE.
JOURNAL OF CLINICAL MEDICINE
(2023)
Article
Obstetrics & Gynecology
Ryan Lee, Luke Witherspoon, Meghan Robinson, Jeong Hyun Lee, Simon P. Duffy, Ryan Flannigan, Hongshen Ma
Summary: A machine learning algorithm was developed to detect rare human sperm in semen and microsurgical testicular sperm extraction (microTESE) samples using bright-field (BF) microscopy for nonobstructive azoospermia patients. The algorithm achieved high precision and sensitivity in both validation and testing stages.
FERTILITY AND STERILITY
(2022)
Article
Urology & Nephrology
Khaled Almekaty, Mohamed H. Zahran, Ahmed Eid, David Ralph, Ayman Rashed
Summary: This study aimed to assess the risk of azoospermia development and the value of sperm retrieval in post-pubertal testicular torsion (TT). The results showed that successful sperm retrieval was achieved in most patients, but after 6 months, 33.8% of patients developed azoospermia.
Article
Obstetrics & Gynecology
Jitske Eliveld, Ilias van der Bles, Madelon van Wely, Andreas Meissner, Alexandre T. Soufan, Annemieke C. Heijboer, Sjoerd Repping, Fulco van der Veen, Ans M. M. van Pelt
Summary: This study investigates the risk of hypogonadism in men with obstructive azoospermia, non-obstructive azoospermia (NOA) or Klinefelter syndrome after testicular sperm extraction (TESE). The results show that 36% of men with Klinefelter syndrome, 4% of men with obstructive azoospermia, and 3% of men with NOA required testosterone replacement therapy (TRT). Klinefelter syndrome is strongly associated with the need for TRT, while no association is found for obstructive azoospermia or NOA. Additionally, higher testosterone concentrations before TESE are linked to a lower chance of needing TRT.
REPRODUCTIVE BIOMEDICINE ONLINE
(2023)
Article
Andrology
Mohammed Saber-Khalaf, Atef F. Ali, Omar M. Elsoghier
Summary: This study aimed to assess the predictive factors of successful sperm retrieval in non-obstructive azoospermia in patients with a history of bilateral cryptorchidism. The results showed that testicular volume and serum testosterone levels were independent factors for successful sperm retrieval.
Article
Urology & Nephrology
Aaron Brant, Nahid Punjani, Nikica Zaninovic, Gianpiero D. Palermo, Zev Rosenwaks, Peter N. Schlegel
Summary: Cancer treatments such as chemotherapy and radiation may result in male infertility, but chemotherapy alone may have better outcomes for fertility preservation compared to combined chemotherapy and radiation. Pelvic radiation therapy is associated with the worst prognosis for successful treatment with microsurgical sperm retrieval and in vitro fertilization.
JOURNAL OF UROLOGY
(2022)
Article
Endocrinology & Metabolism
Tianwen Peng, Chen Liao, Xin Ye, Zhicong Chen, Yu Lan, Xin Fu, Geng An
Summary: This study evaluated the effect of preoperative gonadotropins therapy on sperm retrieval rate (SRR) and ICSI outcomes in non-obstructive azoospermia patients. The results showed that preoperative gonadotropins therapy could significantly improve SRR, but had no effect on clinical outcomes and live birth rate of ICSI.
REPRODUCTIVE BIOLOGY AND ENDOCRINOLOGY
(2022)
Article
Andrology
Chara Oraiopoulou, Aimilia Vorniotaki, Eleni Taki, Achilleas Papatheodorou, Nicos Christoforidis, Alexia Chatziparasidou
Summary: The study found that the total quality of testicular tissue and rare presence of spermatozoa significantly affected the fertilisation rate, while factors such as total tissue quality, cryopreservation of low-quality samples, absence of motile spermatozoa, and poor spermatozoa morphology had a negative impact on the number of good quality embryos. However, clinical pregnancy rate, ongoing pregnancy rate, and live birth rate were not affected by the parameters examined in the study.
Review
Medicine, General & Internal
Nahid Punjani, Caroline Kang, Peter N. Schlegel
Summary: Advancements in surgical sperm retrieval methods, particularly the development of mTESE technology, have significantly improved sperm retrieval rates for men with NOA over the past two decades. Limited predictive factors for successful sperm retrieval emphasize the importance of surgeon experience, patient optimization, and laboratory teamwork in achieving successful outcomes.
JOURNAL OF CLINICAL MEDICINE
(2021)
Article
Obstetrics & Gynecology
F. Ghieh, A. L. Barbotin, N. Swierkowski-Blanchard, C. Leroy, J. Fortemps, C. Gerault, C. Hue, H. Mambu Mambueni, S. Jaillard, M. Albert, M. Bailly, V Izard, D. Molina-Gomes, F. Marcelli, J. Prasivoravong, V Serazin, M. N. Dieudonne, M. Delcroix, H. J. Garchon, A. Louboutin, B. Mandon-Pepin, S. Ferlicot, F. Vialard
Summary: Whole-exome sequencing in combination with testicular sperm extraction may provide additional information and predict the outcome of recurrent TESE in men with maturation arrest. The study suggests that WES screening could help avoid recurrent TESE in men with maturation arrest, particularly in individuals with consanguineous background.
HUMAN REPRODUCTION
(2022)
Article
Urology & Nephrology
Mohammad H. Alkandari, Joseph Moryousef, Simon Phillips, Armand Zini
Summary: In men with severe oligozoospermia, TESA and micro-TESE are equally successful sperm retrieval techniques, while in men with cryptozoospermia, sperm retrieval rates are significantly higher with micro-TESE than TESA.
