期刊
PATHOLOGY RESEARCH AND PRACTICE
卷 205, 期 6, 页码 375-385出版社
ELSEVIER GMBH
DOI: 10.1016/j.prp.2008.08.009
关键词
E-cadherin; Breast cancer invasion; Myoepithelial cell layer disruption; Epithelial-stromal-interaction; Double immunohistochemistry
类别
资金
- Ministry of Chinese Science and Technology Department [2006CB910505]
- US Congressionally Directed Medical Research Programs [DAMD 17-01-1-0129, DAMD17-01-1-0130, PC051308]
- The Susan G. Komen Breast Cancer Foundation [BCTR0706983]
- AFIP/ARP [05AA]
Our recent studies showed that cell clusters overlying focal myoepithelial cell layer disruptions (FMCLD) had a significantly higher rate of ER negativity, genetic instabilities, and expression of invasion-related genes than adjacent cells within the same duct. This study attempted to determine if these cells would show aberrant E-cadherin expression, which imparts greater propensity for cell motility and invasion. Consecutive sections from breast tumors with a high frequency of FMCLD were double-immunostained for E-cadherin and a panel of related markers. The E-cadherin mRNA levels in cells overlying FMCLD and adjacent cells within the same duct were compared using real-time PCR. Nearly all the cell clusters overlying FMCLD were strongly immunoreactive for E-cadherin, whereas their adjacent counterparts within the same duct were largely negative. Cell clusters overlying FMCLD were generally arranged as tongue-like projections, puncturing deep into the stroma or tube-like structures that often contained red blood cells. The sub-cellular localization of E-cadherin in the above structures, however, was primarily cytoplasmic. The mRNA level of E-cadherin in cell clusters overlying FMCLD was significantly higher than that in adjacent cells within the same duct. These findings suggest that aberrant expression of E-cadherin may contribute to cell motility and invasion. Published by Elsevier GmbH.
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