Molecular scissors and their application in genetically modified farm animals

Use of the CRISPR/Cas9 System to Produce Genetically Engineered Pigs from In Vitro-Derived Oocytes and Embryos1

(2014) Kristin M. Whitworth et al.   BIOLOGY OF REPRODUCTION

One-step generation of knockout pigs by zygote injection of CRISPR/Cas system

(2014) Tang Hai et al.   CELL RESEARCH

Improving CRISPR-Cas nuclease specificity using truncated guide RNAs

(2014) Yanfang Fu et al.   NATURE BIOTECHNOLOGY

Dimeric CRISPR RNA-guided FokI nucleases for highly specific genome editing

(2014) Shengdar Q Tsai et al.   NATURE BIOTECHNOLOGY

Efficient genome modification by CRISPR-Cas9 nickase with minimal off-target effects

(2014) Bin Shen et al.   NATURE METHODS

Genome edited sheep and cattle

(2014) Chris Proudfoot et al.   TRANSGENIC RESEARCH

Factors influencing the efficiency of generating genetically engineered pigs by nuclear transfer: multi-factorial analysis of a large data set

(2013) Mayuko Kurome et al.   BMC BIOTECHNOLOGY

One-Step Generation of Mice Carrying Mutations in Multiple Genes by CRISPR/Cas-Mediated Genome Engineering

(2013) Haoyi Wang et al.   CELL

One-Step Generation of Mice Carrying Reporter and Conditional Alleles by CRISPR/Cas-Mediated Genome Engineering

(2013) Hui Yang et al.   CELL

Genetically modified pigs to model human diseases

(2013) Tatiana Flisikowska et al.   JOURNAL OF APPLIED GENETICS

A library of TAL effector nucleases spanning the human genome

(2013) Yongsub Kim et al.   NATURE BIOTECHNOLOGY

Generation of Interleukin-2 Receptor Gamma Gene Knockout Pigs from Somatic Cells Genetically Modified by Zinc Finger Nuclease-Encoding mRNA

(2013) Masahito Watanabe et al.   PLoS One

Efficient nonmeiotic allele introgression in livestock using custom endonucleases

(2013) W. Tan et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

RNA-Guided Human Genome Engineering via Cas9

(2013) P. Mali et al.   SCIENCE

Multiplex Genome Engineering Using CRISPR/Cas Systems

(2013) L. Cong et al.   SCIENCE

Live pigs produced from genome edited zygotes

(2013) Simon G. Lillico et al.   Scientific Reports

RNA-programmed genome editing in human cells

(2013) Martin Jinek et al.   eLife

Gene knockout and knockin by zinc-finger nucleases: current status and perspectives

(2012) J. Hauschild-Quintern et al.   CELLULAR AND MOLECULAR LIFE SCIENCES

Generation of Rag1-knockout immunodeficient rats and mice using engineered meganucleases

(2012) Séverine Ménoret et al.   FASEB JOURNAL

A Porcine Model of Familial Adenomatous Polyposis

(2012) Tatiana Flisikowska et al.   GASTROENTEROLOGY

Targeted gene addition to a predetermined site in the human genome using a ZFN-based nicking enzyme

(2012) J. Wang et al.   GENOME RESEARCH

RNA-guided genetic silencing systems in bacteria and archaea

(2012) Blake Wiedenheft et al.   NATURE

Efficient TALEN-mediated gene knockout in livestock

(2012) D. F. Carlson et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Gender non-specific efficacy of ZFN mediated gene targeting in pigs

(2012) Janet Hauschild-Quintern et al.   TRANSGENIC RESEARCH

Coronary microvascular dysfunction in a porcine model of early atherosclerosis and diabetes

(2011) Mieke van den Heuvel et al.   AMERICAN JOURNAL OF PHYSIOLOGY-HEART AND CIRCULATORY PHYSIOLOGY

CRISPR-Cas Systems in Bacteria and Archaea: Versatile Small RNAs for Adaptive Defense and Regulation

(2011) Devaki Bhaya et al.   Annual Review of Genetics

Highly efficient modification of beta-lactoglobulin (BLG) gene via zinc-finger nucleases in cattle

(2011) Shengli Yu et al.   CELL RESEARCH

Meganucleases and Other Tools for Targeted Genome Engineering: Perspectives and Challenges for Gene Therapy

(2011) George Silva et al.   CURRENT GENE THERAPY

CRISPR-based adaptive immune systems

(2011) Michael P Terns et al.   CURRENT OPINION IN MICROBIOLOGY

Species-Specific Variation in RELA Underlies Differences in NF- B Activity: a Potential Role in African Swine Fever Pathogenesis

(2011) C. J. Palgrave et al.   JOURNAL OF VIROLOGY

Move over ZFNs

(2011)   NATURE BIOTECHNOLOGY

Heritable gene targeting in zebrafish using customized TALENs

(2011) Peng Huang et al.   NATURE BIOTECHNOLOGY

Genetic engineering of human pluripotent cells using TALE nucleases

(2011) Dirk Hockemeyer et al.   NATURE BIOTECHNOLOGY

Targeted gene disruption in somatic zebrafish cells using engineered TALENs

(2011) Jeffry D Sander et al.   NATURE BIOTECHNOLOGY

Knockout rats generated by embryo microinjection of TALENs

(2011) Laurent Tesson et al.   NATURE BIOTECHNOLOGY

Efficient design and assembly of custom TALEN and other TAL effector-based constructs for DNA targeting

(2011) Tomas Cermak et al.   NUCLEIC ACIDS RESEARCH

Modularly assembled designer TAL effector nucleases for targeted gene knockout and gene replacement in eukaryotes

(2011) Ting Li et al.   NUCLEIC ACIDS RESEARCH

Efficient Immunoglobulin Gene Disruption and Targeted Replacement in Rabbit Using Zinc Finger Nucleases

(2011) Tatiana Flisikowska et al.   PLoS One

Efficient generation of a biallelic knockout in pigs using zinc-finger nucleases

(2011) J. Hauschild et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

De novo-engineered transcription activator-like effector (TALE) hybrid nuclease with novel DNA binding specificity creates double-strand breaks

(2011) M. M. Mahfouz et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Pig gene knockout by rAAV-mediated homologous recombination: comparison of BRCA1 gene knockout efficiency in Yucatan and Göttingen fibroblasts with slightly different target sequences

(2011) Yonglun Luo et al.   TRANSGENIC RESEARCH

XanthomonasAvrBs3 Family-Type III Effectors: Discovery and Function

(2010) Jens Boch et al.   Annual Review of Phytopathology

Knockout of exogenous EGFP gene in porcine somatic cells using zinc-finger nucleases

(2010) Masahito Watanabe et al.   BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS

Targeted Genome Modification in Mice Using Zinc-Finger Nucleases

(2010) I. D. Carbery et al.   GENETICS

Targeting DNA Double-Strand Breaks with TAL Effector Nucleases

(2010) M. Christian et al.   GENETICS

Gene targeting with zinc finger nucleases to produce cloned eGFP knockout pigs

(2010) Jeffrey J. Whyte et al.   MOLECULAR REPRODUCTION AND DEVELOPMENT

Targeted integration in rat and mouse embryos with zinc-finger nucleases

(2010) Xiaoxia Cui et al.   NATURE BIOTECHNOLOGY

A TALE nuclease architecture for efficient genome editing

(2010) Jeffrey C Miller et al.   NATURE BIOTECHNOLOGY

Transient cold shock enhances zinc-finger nuclease–mediated gene disruption

(2010) Yannick Doyon et al.   NATURE METHODS

Zinc-finger nuclease-driven targeted integration into mammalian genomes using donors with limited chromosomal homology

(2010) Salvatore J. Orlando et al.   NUCLEIC ACIDS RESEARCH

TAL nucleases (TALNs): hybrid proteins composed of TAL effectors and FokI DNA-cleavage domain

(2010) Ting Li et al.   NUCLEIC ACIDS RESEARCH

Gene targeting by homologous recombination in mouse zygotes mediated by zinc-finger nucleases

(2010) M. Meyer et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

The I-CreI meganuclease and its engineered derivatives: applications from cell modification to gene therapy

(2010) S. Arnould et al.   PROTEIN ENGINEERING DESIGN & SELECTION

The immense potential of xenotransplantation in surgery

(2010) David K.C. Cooper et al.   International Journal of Surgery

Utilization of an unstable plasmid and the I-SceI endonuclease to generate routine markerless deletion mutants in Francisella tularensis

(2009) Joseph Horzempa et al.   JOURNAL OF MICROBIOLOGICAL METHODS

Efficient targeting of a SCID gene by an engineered single-chain homing endonuclease

(2009) Sylvestre Grizot et al.   NUCLEIC ACIDS RESEARCH

A muscle-specific transgenic reporter line of the sea anemone, Nematostella vectensis

(2009) E. Renfer et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

DNA Binding Made Easy

(2009) D. F. Voytas et al.   SCIENCE

Breaking the Code of DNA Binding Specificity of TAL-Type III Effectors

(2009) J. Boch et al.   SCIENCE

A Simple Cipher Governs DNA Recognition by TAL Effectors

(2009) M. J. Moscou et al.   SCIENCE

Knockout Rats via Embryo Microinjection of Zinc-Finger Nucleases

(2009) Aron M. Geurts et al.   SCIENCE

A system for the construction of targeted unmarked gene deletions in the genus Burkholderia

(2008) Ronald S. Flannagan et al.   ENVIRONMENTAL MICROBIOLOGY

Zinc-finger Nucleases: The Next Generation Emerges

(2008) Toni Cathomen et al.   MOLECULAR THERAPY

Heritable targeted gene disruption in zebrafish using designed zinc-finger nucleases

(2008) Yannick Doyon et al.   NATURE BIOTECHNOLOGY

Targeted gene inactivation in zebrafish using engineered zinc-finger nucleases

(2008) Xiangdong Meng et al.   NATURE BIOTECHNOLOGY

Rapid and efficient construction of markerless deletions in the Escherichia coli genome

(2008) B. J. Yu et al.   NUCLEIC ACIDS RESEARCH

Disruption of the CFTR Gene Produces a Model of Cystic Fibrosis in Newborn Pigs

(2008) C. S. Rogers et al.   SCIENCE

Development and Validation of a Highly Efficient Protocol of Porcine Somatic Cloning Using Preovulatory Embryo Transfer in Peripubertal Gilts

(2008) Björn Petersen et al.   Cloning Stem Cells