期刊
PARASITOLOGY RESEARCH
卷 113, 期 7, 页码 2535-2540出版社
SPRINGER
DOI: 10.1007/s00436-014-3903-z
关键词
18S rDNA; Centrocestus formosanus; Collyriclum faba; Flukes; Haplorchis pumilio; Haplorchis taichui; Lecithodendriidae sp; Lissorchus kritskyi; Opecoelidae sp; Paratanaisia bragai; Philophthalmus sp; Pleurogenes claviger; Sanguinicola cf. inermis; Transversotrema haasi
类别
资金
- United States-Israel Binational Science Foundation (BSF) [2011011]
- Israel Taxonomy Initiative
Trematodes are significant pathogens of high medical, veterinary, and environmental importance. They are hard to isolate from their intermediate hosts, and their early life stages are difficult to identify morphologically. Therefore, primers were developed for trematodes to create a species barcoding system and allow selective PCR amplification in mixed samples. The specific oligonucleotide primer was universal for trematodes that infected several freshwater snail species in Israel. The diagnostic tool is based on the 18S rDNA gene. In contrast to morphological identification, trematode barcoding is rapid as it is based on a sequence of only 800 bp, and it classifies species accurately due to high polymorphism between conserved areas.
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