Article
Veterinary Sciences
Lukas Demattio, Ivan Conejeros, Daniela Grob, Ulrich Gaertner, Anja Taubert, Carlos Hermosilla, Axel Wehrend
Summary: Colostrum is essential for the health and development of mammalian neonates, and it has been found that polymorphonuclear neutrophils (PMN) migrate from the mother to the infant via colostrum uptake. However, little is known about the activities of colostral PMN in sheep. This study examined the ability of ovine colostral-derived PMN to extrude neutrophil extracellular traps (NETs) against the abortive apicomplexan parasite Neospora caninum.
FRONTIERS IN VETERINARY SCIENCE
(2023)
Review
Veterinary Sciences
Luis F. Pita Gondim, Milton M. McAllister
Summary: Experimental infections of pregnant cows with Neospora caninum have provided valuable information on host-parasite interaction and immunopathogenesis. Most studies use tachyzoites as the inoculum, while only a few studies have used oocysts. This mini-review discusses the differences between tachyzoites and oocysts as inocula and the route of inoculation in pregnant cows.
FRONTIERS IN VETERINARY SCIENCE
(2022)
Article
Immunology
Yanina P. Hecker, Mercedes M. Burucua, Franco Fiorani, Jaime E. Maldonado Rivera, Karina M. Cirone, Matias A. Dorsch, Felipe A. Cheuquepan, Lucia M. Campero, German J. Canton, Maia S. Marin, Luis M. Ortega-Mora, Dadin P. Moore
Summary: This study evaluated the reactivation and fetal infection in pregnant heifers inoculated with live Neospora caninum tachyzoites before puberty. The results showed that animals infected before puberty experienced reactivation of the infection at the seventh month of gestation, and congenital infection was confirmed in calves born from these animals. This study provides evidence that using live N. caninum tachyzoites in young animals is neither safe nor effective.
Article
Immunology
Monica L. Mazuz, Benjamin Leibovitz, Igor Savitsky, Elena Blinder, Daniel Yasur-Landau, Yaniv Lavon, Binyamin Sharir, Sharon Tirosh-Levy
Summary: Vaccination with frozen live Neospora caninum tachyzoites was found to reduce abortion rates in naturally infected seropositive pregnant dairy dams, particularly in cows with high antibody titers. The vaccine efficacy varied among different farms, but overall, the frozen live vaccination may be an effective method to control neosporosis in cattle.
Article
Microbiology
Jessica Ramseier, Dennis Imhof, Kai Pascal Alexander Haenggeli, Nicoleta Anghel, Ghalia Boubaker, Richard M. Beteck, Luis-Miguel Ortega-Mora, Richard K. K. Haynes, Andrew Hemphill
Summary: The effects of decoquinate and three O-quinoline-carbamate-derivatives were investigated on human foreskin fibroblasts infected with Neospora caninum tachyzoites. These compounds exhibited different levels of proliferation inhibition and did not affect cell viability at certain concentrations. Treatment with lower concentrations altered the ultrastructure of the parasite, while long-term treatment with one compound showed parasiticidal effects. These compounds were then tested in a murine model, but did not show activity against neosporosis.
Article
Parasitology
Gang Liu, Lijun Jia, Qingyan Shao, Huijun Lu, Jixue Zhao, Jigang Yin
Summary: This study identified a significant number of novel miRNAs from N. caninum tachyzoites using high-throughput RNA sequencing technology and systematic bioinformatics analysis. Among 300 miRNAs identified, 10 were conserved miRNAs belonging to metazoan miRNA families while 290 were novel miRNAs. The expression of 13 novel miRNAs was validated through real-time quantitative PCR, providing important data for the study of miRNAs in N. caninum.
PARASITOLOGY RESEARCH
(2021)
Article
Parasitology
Roberto Sanchez-Sanchez, Ignacio Ferre, Michela Re, Barbara Perez-Arroyo, Dario Cleofe-Resta, Victor Herrero Garcia, Manuel Pizarro Diaz, Luis Miguel Ferrer, Hector Ruiz, Raquel Vallejo-Garcia, Julio Benavides, Matthew A. Hulverson, Ryan Choi, Grant R. Whitman, Andrew Hemphill, Wesley C. Van Voorhis, Luis Miguel Ortega-Mora
Summary: The study demonstrates the efficacy of BKI-1294 in treating sheep infected with Nc-Spain7 tachyzoites, although it did not prevent abortion or reduce the risk of fetal infection.
INTERNATIONAL JOURNAL FOR PARASITOLOGY-DRUGS AND DRUG RESISTANCE
(2021)
Article
Parasitology
Jin-Ming Chen, Shan-Shan Zhao, De-Liang Tao, Jing-Yu Li, Xin Yang, Ying-Ying Fan, Jun-Ke Song, Qun Liu, Guang-Hui Zhao
Summary: This study reveals that the expression of host miRNAs changes during N. caninum infection, and the upregulation of chi-miR-146a may promote the propagation of N. caninum in host cells.
PARASITES & VECTORS
(2023)
Article
Parasitology
Rafaela Furioso Ferreira, Rosangela Locatelli Dittrich, Isabelle Bay Zimmermann, Blanka Beer Ljubic, Vladimir Mrljak, Peter David Eckersall
Summary: This study evaluated the acute phase protein response in dogs seropositive and seronegative for N. caninum. The results showed that seropositive dogs with symptoms had higher levels of Hp, while seronegative dogs had higher CRP levels. However, there was no difference in CRP concentration in asymptomatic dogs.
PARASITOLOGY RESEARCH
(2021)
Article
Biochemistry & Molecular Biology
Heloise Debare, Nathalie Moire, Celine Ducournau, Joerg Schmidt, Jan-David Laakmann, Ralph T. Schwarz, Isabelle Dimier-Poisson, Francoise Debierre-Grockiego
Summary: Neospora caninum causes abortion in ruminants, and there is currently no effective treatment available, so future vaccine formulations should aim to stimulate immune responses through a wide panel of antigens and adjuvants. A mucosal vaccine loaded with N. caninum TE and GPI has shown efficacy in reducing parasite burden, suggesting potential adjuvant properties for a vaccine strategy against neosporosis.
Article
Parasitology
Dennis Imhof, Nicoleta Anghel, Pablo Winzer, Vreni Balmer, Jessica Ramseier, Kai Haenggeli, Ryan Choi, Matthew A. Hulverson, Grant R. Whitman, Samuel L. M. Arnold, Kayode K. Ojo, Wesley C. Van Voorhis, J. Stone Doggett, Luis M. Ortega-Mora, Andrew Hemphill
Summary: Bumped kinase inhibitors (BKIs) target apicomplexan parasites by inhibiting their proliferation, with BKI1748 showing promising results in both in vitro and animal models. The compound was safe and effective in pregnant mice, and demonstrated inhibition of vertical transmission and reduced parasite loads in non-pregnant mice. BKI-1748 also showed potential in reducing ocular infection in a Toxoplasma gondii model. Overall, BKI-1748 presents itself as a promising candidate for further research on neosporosis and toxoplasmosis in larger animal models.
INTERNATIONAL JOURNAL FOR PARASITOLOGY-DRUGS AND DRUG RESISTANCE
(2021)
Article
Microbiology
Sabrina Mazouz, Eduardo Salinas, Nathalie Bedard, Ali Filali, Omar Khedr, Leo Swadling, Mohamed S. Abdel-Hakeem, Asiyah Siddique, Eleanor Barnes, Julie Bruneau, Arash Grakoui, Naglaa H. Shoukry
Summary: In this study, the memory immune response against hepatitis C virus (HCV) upon re-exposure and reinfection followed by spontaneous clearance was examined. The results suggest that new vaccines against HCV should combine both antibodies and T cells, as they play a cooperative role in protection against HCV.
Article
Biochemistry & Molecular Biology
Angela C. de O. Lima, Rodrigo S. Conceicao, Luciana S. Freitas, Carlos A. L. de Carvalho, Antonio L. da S. Conceicao, Humberto F. Freitas, Samuel S. da R. Pita, Demian R. Ifa, Alexandre M. Pinheiro, Alexsandro Branco
Summary: The study identified four hydroxycinnamic acid-spermidine amides (HCAAs) in fractions B and C of Tetragonisca angustula honey, which showed parasitic reduction without cell toxicity. In silico studies suggest that HCAAs may affect the parasite's redox pathway and improve the oxidative effect of NO released from infected cells.
CHEMICAL BIOLOGY & DRUG DESIGN
(2021)
Article
Biochemical Research Methods
Leslie J. Mateo, Nasa Sinnott-Armstrong, Alistair N. Boettiger
Summary: The paper presents a microscopy approach called Optical Reconstruction of Chromatin Architecture (ORCA) for tracing the 3D DNA path within the nuclei of fixed tissues and cultured cells, achieving fine genomic resolution as Hi-C and single-cell resolution with multimodal measurements.
Article
Cell Biology
Xue-Yan Zhang, Jun Li, Cai-Juan Li, Ying-Qi Lin, Chun-Hui Huang, Xiao Zheng, Xi-Chen Song, Zhu-Chi Tu, Xiao-Jiang Li, Sen Yan
Summary: The developmental and maturation pace of primary cerebral cortical neurons from cynomolgus monkeys is slower compared to mice, and cultured monkey cortical neurons exhibit typical features of Huntington's disease when infected with a mutant HTT. This suggests that cultured cynomolgus monkey neurons may serve as a cell model for studying human neurodegenerative diseases and neuronal regeneration.
NEURAL REGENERATION RESEARCH
(2021)
Article
Microbiology
Thach Xuan Tran, Trang Thu Le, Long Phi Trieu, Christopher M. Austin, Dong Van Quyen, Huong Minh Nguyen
ANNALS OF CLINICAL MICROBIOLOGY AND ANTIMICROBIALS
(2019)
Article
Multidisciplinary Sciences
Thanh Thi Minh Le, Anh Thi Hong Hoang, Thuy Thi Bich Le, Thuy Thi Bich Vo, Dong Van Quyen, Ha Hoang Chu
SCIENTIFIC REPORTS
(2019)
Article
Biochemistry & Molecular Biology
Le Thi Nhi-Cong, Do Thi Lien, Bhaskar Sen Gupta, Cung Thi Ngoc Mai, Hoang Phuong Ha, Nguyen Thi Minh Nguyet, Tran Hoa Duan, Dong Van Quyen, Hayyiratul Fatimah Mohd Zaid, Revathy Sankaran, Pau Loke Show
APPLIED BIOCHEMISTRY AND BIOTECHNOLOGY
(2020)
Article
Biotechnology & Applied Microbiology
Thanh Thi Minh Le, Anh Thi Hong Hoang, Nhue Phuong Nguyen, Thuy Thi Bich Le, Ha Thi Thu Trinh, Thuy Thi Bich Vo, Dong Van Quyen
BIOTECHNOLOGY LETTERS
(2020)
Article
Genetics & Heredity
Dong Van Quyen, Han Ming Gan, Yin Peng Lee, Dinh Duy Nguyen, Thi Hoa Nguyen, Xuan Thach Tran, Van Sang Nguyen, Dinh Duy Khang, Christopher M. Austin
Article
Biotechnology & Applied Microbiology
Bui Thi Thuy Duong, Nguyen Thi Kim Lien, Ha Thi Thu, Nguyen Thi Hoa, Pham Thi Lanh, Bo-Ram Yun, Mi-Sun Yoo, Yun Sang Cho, Dong Van Quyen
BIOTECHNOLOGY LETTERS
(2020)
Article
Multidisciplinary Sciences
Khanh Minh Chau, Dong Van Quyen, Joshua M. Fraser, Andrew T. Smith, Thi Thu Hao Van, Robert J. Moore
Article
Multidisciplinary Sciences
Ha T. Thu, Nguyen T. K. Lien, Pham T. Lanh, Bui T. T. Duong, Nguyen T. Hoa, Man H. Phuoc, Pham H. Thai, Dong Van Quyen
Article
Fisheries
Pham Thi Lanh, Huong Minh Nguyen, Bui Thi Thuy Duong, Nguyen Thi Hoa, Le Thi Thom, Luu Thi Tam, Ha Thi Thu, Vo Van Nha, Dang Diem Hong, Aidyn Mouradov, Apurav Krishna Koyande, Pau-Loke Show, Dong Van Quyen
Summary: This study shows that expressing the VP28 protein of WSSV in Chlamydomonas reinhardtii has the potential to serve as an oral vaccine candidate against WSSV, significantly increasing survival rates in susceptible shrimp.
Article
Environmental Sciences
Le Thi Nhi-Cong, Do Thi Lien, Cung Thi Ngoc Mai, Nguyen Viet Linh, Nguyen Quang Lich, Hoang Phuong Ha, Dong Van Quyen, Doris Ying Ying Tang, Pau Loke Show
Summary: Oil pollution from industrial activities, particularly the oil and gas industry, is a serious issue. This study evaluated the efficiency of cinder beats, coconut fiber, and polyurethane foam as immobilization carriers for purple phototrophic bacterial strains in biodegrading crude oil contaminated seawater, finding that the coconut fiber-bacteria system showed the highest removal efficiency for crude oil.
Article
Microbiology
Khanh Minh Chau, Thi Thu Hao Van, Dong Van Quyen, Hung Dinh Le, Trinh Hoai Thi Phan, Ngoc Duy Thi Ngo, Trang Dieu Thi Vo, Trung Thanh Dinh, Hoa Thi Le, Huynh Hoang Nhu Khanh
Summary: This study characterized and compared 16 potential anti-Vibrio probiotics isolated from marine sponges and fish intestines, as well as an anti-Vibrio bacteriocin, revealing a higher antimicrobial spectrum in intestinal Vi+ due to the higher gene frequency of two antimicrobial compounds. Additionally, a three-step procedure was used to purify an anti-Vibrio bacteriocin produced by B. methylotrophicus NTBD1, identifying essential properties for promising feed additives. This research provides necessary information on the potential of probiotic Bacillus species with anti-Vibrio antimicrobial properties for sustainable aquaculture.
Article
Multidisciplinary Sciences
Thach Xuan Tran, Nguyen T. K. Lien, Ha T. Thu, Nguyen Dinh Duy, Bui T. T. Duong, Dong Van Quyen
Summary: The study analyzed the complete genome of a PEDV strain in Vietnam 2018 and found multiple mutations that can affect the virus's receptor specificity, pathogenicity, and ability to evade host immune system. The importance of molecular characterization of field strains for the development of effective vaccines to control PEDV infections in Vietnam is highlighted.
Article
Multidisciplinary Sciences
Bo-Ram Yun, A-Tai Truong, Yong Soo Choi, Man Young Lee, Byoung Yong Kim, Minjung Seo, Soon-Seek Yoon, Mi-Sun Yoo, Dong Van Quyen, Yun Sang Cho
Summary: This study investigated the relationship between the gut microbiome of honey bees and sacbrood disease (SD). It was found that sacbrood virus (SBV)-resistant honey bee strains harbor specific acetic acid bacteria and lactic acid bacteria. SBV infection led to the loss of beneficial bacteria in the honey bee gut, which could affect host nutrition and inhibit honey bee pathogens.
SCIENTIFIC REPORTS
(2022)
Article
Virology
Bui Thi Thuy Duong, Pham Thi Phuong Thao, Nguyen Thi Hoa, Ha Thi Thu, Man Hong Phuoc, Thanh Hoa Le, Dong Van Quyen
Summary: The spike protein of porcine epidemic diarrhea virus (PEDV) exhibits a high degree of genetic and antigenic diversity in northern Vietnam. A new antigenic variant subgroup has been identified, highlighting the need for frequent updates of vaccines for effective protection.
ARCHIVES OF VIROLOGY
(2022)
Review
Biochemistry & Molecular Biology
Guowei Wu, Dingling Zhuang, Kit Wayne Chew, Tau Chuan Ling, Kuan Shiong Khoo, Dong Van Quyen, Shuying Feng, Pau Loke Show
Summary: With the development of the economy and productivity, concerns about the nutritional value and safety of algae as a food resource are increasing. Physical, chemical, and microbial hazards have been identified as the main factors affecting the food safety of algae. Microbial contamination is found to be the main cause of foodborne diseases related to algae. Traditional microbiological control techniques are time-consuming and less accurate, highlighting the need for rapid and efficient detection methods. Monitoring of heavy metals, algal toxins, and other pollutants is also important. In the future, algal food safety risk assessment should focus on convenient, rapid, low-cost, and high-accuracy detection methods, and incorporate novel technologies such as Internet of Things, biosensors, and molecular biology.
