期刊
PARASITOLOGY INTERNATIONAL
卷 62, 期 2, 页码 199-207出版社
ELSEVIER IRELAND LTD
DOI: 10.1016/j.parint.2012.12.009
关键词
NEDD8; NEDDylation; Schistosomula development; Differential expression
类别
资金
- Brazilian research agency: FAPEMIG (Fundacao de Amparo a Pesquisa do Estado de Minas Gerais) [CBB 0558/09]
- Brazilian research agency: NuBio UFOP (Nude de Bioinformatica da Universidade Federal de Ouro Preto)
- Brazilian research agency: CNPq (Conselho Nacional de Desenvolvimento Cientifico e Tecnologico)
NEDD8 is an ubiquitin-like molecule that covalently binds to target proteins through an enzymatic cascade analogous to ubiquitylation. This modifier is known to bind to p53 and p73, as well as all Cullin family proteins, which are essential components of Skp1/Cul-1/F-box protein (SCF)-like Ub ligase complexes. Here, we focused on a genomic analysis of the genes involved in the NEDD8 conjugation pathway in Schistosoma mansoni. The results revealed seven genes related to NEDD8 conjugation that are conserved in Schistosoma japonicum, Caenorhabditis elegans, Drosophila melanogaster and Homo sapiens. We performed quantitative RT-PCR (qRT-PCR), which showed differential profiles for Smnedd8, Smapp1, Smuba3, Smube2f, Smdcn1, Smrbx and Smsenp8 throughout the life cycle of S. mansoni. Upregulation was observed in 3-day-old schistosomula and adult worms for all analysed genes. We also analysed the transcription levels of Cullin family members Smp63 and Smp73, and observed upregulation in early schistosomula, while cercariae and adult worms showed expression levels similar to one another. Taken together, these results suggest that the NEDDylation/DeNEDDylation pathway controls important cellular regulators during worm development from cercariae to schistosomula and, finally, to adult. (C) 2013 Elsevier Ireland Ltd. All rights reserved.
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