期刊
PARASITOLOGY INTERNATIONAL
卷 59, 期 1, 页码 54-61出版社
ELSEVIER IRELAND LTD
DOI: 10.1016/j.parint.2009.10.005
关键词
Giardia intestinalis; Excystation; Phosphorylation; 14-3-3; Hsp70
类别
资金
- Universidad Nacional de Colombia [8003154]
The parasite Giardia intestinalis undergoes a differentiation process that allows it to infect its mammal host. That process is excystation. We examined the importance of protein phosphorylation during the passage from cyst to trophozoite. Cysts obtained from patients with giardiasis were excysted in vitro and the soluble cytoplasmic proteins were analyzed during the three phases of the process, using a specific staining for phosphoproteins. We found two phosphorylated proteins and identified them with MALDI-TOF as 14-3-3 and Hsp70. Modifications were detected in both proteins, which could indicate a role in differentiation of the parasite. In addition, the inhibition of serine-threonine kinases during excystation specifically affected the cytokinesis of the excyzoite, thus inhibiting the completion of trophozoite formation. (C) 2009 Elsevier Ireland Ltd. All rights reserved.
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