期刊
PARASITOLOGY INTERNATIONAL
卷 58, 期 3, 页码 270-277出版社
ELSEVIER IRELAND LTD
DOI: 10.1016/j.parint.2009.03.005
关键词
Echinococcus granulosus; Flubendazole; Tubulin gene expression; Glycogen; Malate dehydrogenase; Calcium
类别
资金
- ANPCyT [1017]
- CONICET [02172]
- CONAPRIS
The problem of chemotherapeutic treatments for human echinococcosis has not been completely solved. The benzimiclazole-methylcarbamates (BZD), broad-spectrum antihelminthic agents, such as mebendazole and albendazole are the only drugs licensed for treatment of hydatid cysts. These drugs bind directly to beta-tubulin causing the disruption of microtubule-based processes in helminths. However, the molecular bases of their multiple biological activities are poorly understood. Recently, the effect of halogenated derivative flubendazole (FLBZ), against E granulosus larvae has been conclusively demonstrated. The comparative effectiveness of FLBZ, among other BZDs, was shown by means of vitality tests and time of appearance of morphological damage of larvae. In the present study, we examined biochemical and molecular changes on protoscoleces treated with FLBZ. We show that FLBZ induces: 1) an increase in cytosolic free calcium, 2) a decrease in tubulin transcripts, 3) a reduction of mMDH expression and 4) a significant decrease in glycogen levels. These results are consistent with the existence of multiple targets for FLBZ, such as calcium signaling and energy metabolism, and contribute to the understanding of the pharmaceutical properties of FLBZ. (C) 2009 Elsevier Ireland Ltd. All rights reserved.
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