Article
Microbiology
Esther Blanco-Paniagua, Laura Alvarez-Fernandez, Andrea Rodriguez-Alonso, Alicia Millan-Garcia, Ana I. Alvarez, Gracia Merino
Summary: Clorsulon is an effective drug for treating helminthic zoonoses and has high broad-spectrum antiparasitic efficacy when used in combination with ivermectin. The safety and efficacy of clorsulon should be studied by considering drug-drug interactions mediated by ABC transporters. This study demonstrates that clorsulon is actively secreted into milk by ABCG2 transporter and its secretion into milk is reduced by coadministration with ivermectin.
ANTIMICROBIAL AGENTS AND CHEMOTHERAPY
(2023)
Article
Parasitology
Laura Mate, Mariana Ballent, Candela Canton, Carlos Lanusse, Laura Ceballos, Luis L. Ignacio Alvarez, Juan Pedro Liron
Summary: This study compared the gene expression levels of ABC transporters in sheep nematode Haemonchus contortus isolates with different susceptibility to ivermectin and found that some ABC transporter genes showed minor differences in transcription levels, but could not fully explain ivermectin resistance. Pgp-9 may be one of the most relevant candidates contributing to the multi-genic nature of ivermectin resistance.
VETERINARY PARASITOLOGY
(2022)
Article
Food Science & Technology
Roi Martinez-Escauriaza, Vanessa Lozano, M. Luz Perez-Paralle, Juan Blanco, Jose L. Sanchez, Antonio J. Pazos
Summary: The study identified, cloned, and characterized two complete cDNAs of mdr1 and mdr2 genes in the mussel Mytilus galloprovincialis, demonstrating their possible involvement in detoxification processes of toxins. The results suggest that P-gp and MRP proteins may play a crucial role in defending against xenobiotics and resisting DSP toxins in aquatic organisms.
Article
Biochemistry & Molecular Biology
Jianyi Li, Yuntong Lv, Kunpeng Yan, Fengting Yang, Xuewei Chen, Xiwu Gao, Shuyuan Wen, Hongfei Xu, Yiou Pan, Qingli Shang
Summary: The study revealed that ATP-binding cassette transporters (ABCs) play important roles in cyantraniliprole resistance. Inhibition of ABCs increased the toxicity of cyantraniliprole, while overexpression of ABCs in fruit flies enhanced their tolerance to cyantraniliprole. Suppression of specific ABC transporters increased the sensitivity of cyantraniliprole-resistant strains. These findings provide valuable insights for resistance management strategies.
PESTICIDE BIOCHEMISTRY AND PHYSIOLOGY
(2022)
Review
Environmental Sciences
Ahmed Elfadadny, Hussein M. El-Husseiny, Amira Abugomaa, Rokaia F. Ragab, Eman A. Mady, Mohamed Aboubakr, Haney Samir, Ahmed S. Mandour, Amany El-Mleeh, Ali H. El-Far, Ayman H. Abd El-Aziz, Mohamed Elbadawy
Summary: Cancer treatment is a significant public health issue, with drug resistance being a major challenge. Researchers are exploring modulators of multidrug resistance-associated proteins and proposing models that may lay the foundation for further investigation into cancer multidrug resistance.
ENVIRONMENTAL SCIENCE AND POLLUTION RESEARCH
(2021)
Review
Pharmacology & Pharmacy
Jose Pedro Gil, Claudia Fancony
Summary: The lethal Plasmodium falciparum parasite's ability to develop resistance against anti-malarial drugs is a central challenge in global malaria control and elimination efforts. Drug transporters like MRPs play a pivotal role in this process by handling a wide range of substrates, potentially contributing to the development of multi-drug resistance phenotypes.
FRONTIERS IN PHARMACOLOGY
(2021)
Review
Pharmacology & Pharmacy
Jing-Quan Wang, Yuqi Yang, Chao-Yun Cai, Qiu-Xu Teng, Qingbin Cui, Jun Lin, Yehuda G. Assaraf, Zhe-Sheng Chen
Summary: ABC transporters mediate the translocation of structurally and mechanistically distinct substrates against steep concentration gradients using ATP energy. The ABCC subfamily is the largest in humans, with 13 members, including 9 multidrug resistance proteins that can extrude chemotherapeutic agents from tumor cells. Additionally, MRPs are also involved in the efflux of physiologically important organic anions and are potential targets for overcoming cancer multidrug resistance.
DRUG RESISTANCE UPDATES
(2021)
Article
Microbiology
Magali Van den Kerkhof, Philippe Leprohon, Dorien Mabille, Sarah Hendrickx, Lindsay B. Tulloch, Richard J. Wall, Susan Wyllie, Eric Chatelain, Charles E. Mowbray, Stephanie Braillard, Marc Ouellette, Louis Maes, Guy Caljon
Summary: Oxaboroles, a novel class of antileishmanial drugs, have not shown readily emergent resistance and hold promise for future treatment. The combination of a genome-wide cosmid library and next-generation sequencing can identify resistance determinants and potential targets.
Article
Agriculture, Multidisciplinary
Yuntong Lv, Kunpeng Yan, Xiwu Gao, Xuewei Chen, Jianyi Li, Yaping Ding, Hang Zhang, Yiou Pan, Qingli Shang
Summary: This study explores the involvement of ATP-binding cassette (ABC) transporters in spirotetramat resistance in cotton aphids. The results show that several ABC transporter genes, including ABCB4, ABCB5, ABCF2, MRP11, and MRP12, are upregulated in the spirotetramat-resistant strains. Functional analysis confirms the contribution of these genes to spirotetramat tolerance.
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
(2022)
Article
Chemistry, Analytical
Yanyan Ma, Zhanchen Guo, Chuanwen Fan, Jingran Chen, Shuxin Xu, Zhen Liu
Summary: This study developed aptamers that can target ABCG2 and reverse multidrug resistance, showing great potential in cancer diagnosis and treatment. The aptamers can specifically bind to human colorectal cancer stem cells and effectively reverse drug resistance in liver cancer cells.
ANALYTICAL CHEMISTRY
(2022)
Article
Microbiology
Jan Viglas, Petra Olejnikova
Summary: The ABC transporter superfamily plays a crucial role in transporting various molecules across biological membranes, including antifungal compounds in filamentous fungi. Understanding the physiological substrates and roles of individual subfamilies of ABC transporters in fungi is important for clarifying their significance in clinical and agricultural settings.
MICROBIOLOGICAL RESEARCH
(2021)
Article
Biochemistry & Molecular Biology
Yuntong Lv, Yiou Pan, Jianyi Li, Yaping Ding, Zihan Yu, Kunpeng Yan, Qingli Shang
Summary: Clarifying the molecular mechanisms of cotton aphid resistance to various insecticides is crucial for safe insecticide application. This study reveals that ABCB5, ABCF2, and MRP12 are negatively regulated by the transcription factor CF2-II, leading to increased resistance to certain insecticides. These findings contribute to a better understanding of the adaptation mechanism of ABC transporters in the context of multi-insecticide resistance.
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
(2023)
Article
Plant Sciences
Georgios Sofianos, Anastasios Samaras, Georgios Karaoglanidis
Summary: Botrytis cinerea is a high-risk pathogen for fungicide resistance development. The study found that MLR/MDR strains are present in Greek populations of strawberries, rootstocks, and tomatoes, with notably high frequencies of resistance. The combination of MLR and MDR mutations leads to even higher levels of fungicide resistance.
FRONTIERS IN PLANT SCIENCE
(2023)
Article
Pharmacology & Pharmacy
Lena Radtke, Aleksandra Majchrzak-Celinska, Charles Awortwe, Inga Vater, Inga Nagel, Susanne Sebens, Ingolf Cascorbi, Meike Kaehler
Summary: This study demonstrates the significance of ABCB1 in GBM cells, affecting the response to temozolomide and carmustine treatment, while ABCG2 plays a marginal role.
PHARMACOLOGICAL RESEARCH
(2022)
Article
Pharmacology & Pharmacy
Danijela D. Nikodijevic, Jovana V. Jovankic, Danijela M. Cvetkovic, Marko Z. Andelkovic, Aleksandra G. Nikezic, Milena G. Milutinovic
Summary: The study evaluated the potential antitumor activity of snake venom and L-amino acid oxidase on colorectal cancer cells, showing that snake venom primarily led to necrosis while L-amino acid oxidase induced apoptosis correlated with oxidative imbalances in cancer cells. Additionally, the gene expression profiles and levels of membrane transporters also impacted the sensitivity of treatment across different cell lines. The findings suggest that L-amino acid oxidase could be a promising substance for further research on its antitumor effects, potentially with lower risk of drug resistance in vitro.
EUROPEAN JOURNAL OF PHARMACOLOGY
(2021)
Article
Microbiology
Rahul Tyagi, Christina A. Bulman, Fidelis Cho-Ngwa, Chelsea Fischer, Chris Marcellino, Michelle R. Arkin, James H. McKerrow, Case W. McNamara, Matthew Mahoney, Nancy Tricoche, Shabnam Jawahar, James W. Janetka, Sara Lustigman, Judy Sakanari, Makedonka Mitreva
Summary: 18 hits with anti-macrofilaricidal activity were identified, with azoles and aspartic protease inhibitors being prioritized for further study. These drugs showed activity against Onchocerca spp. as well, with the potential to identify selective drugs that prevent adverse events in co-infected individuals.
Correction
Veterinary Sciences
J. Charlier, L. Rinaldi, V. Musella, H. W. Ploeger, C. Chartier, H. Rose Vineer, B. Hinney, G. von Samson-Himmelstjerna, B. Bacescu, M. Mickiewicz, T. L. Mateus, M. Martinez-Valladares, S. Quealy, H. Azaizeh, B. Sekovska, H. Akkari, S. Petkevicius, L. Hektoen, J. Hoglund, E. R. Morgan, D. J. Bartley, E. Claerebout
PREVENTIVE VETERINARY MEDICINE
(2021)
Article
Veterinary Sciences
Carla Mingolla, Wouter van Mol, Liselot Hudders, Veroline Cauberghe, Edwin Claerebout
Summary: Farmers' adoption intention of diagnostic tools is influenced by beliefs and behavioral biases such as positive attitudes towards acaricides use, risk perception of acaricide resistance, economic reasons, and optimism bias. Veterinarians play a key role in implementing diagnostic tools, while the opinions of colleague-farmers also affect decision-making.
PREVENTIVE VETERINARY MEDICINE
(2021)
Article
Parasitology
M. Vanhecke, J. Charlier, C. Strube, E. Claerebout
Summary: This study aimed to assess the impact of lungworm infections on productivity in dairy cows and identify risk factors for infections in grazing dairy cattle. The presence of D. viviparus antibodies in bulk tank milk samples was evaluated on 717 and 634 farms at two-week intervals during two grazing seasons, with associations found between milk antibody levels, production data, and risk factors in herd management. Results indicated potential for the ELISA to identify herds with significant production losses due to lungworm infections, with purchase of new animals and preventive anthelmintic treatment positively associated with milk yield losses, while mowing pastures was negatively associated. Adjustments in farm management could potentially mitigate these losses.
VETERINARY PARASITOLOGY
(2021)
Article
Agriculture, Dairy & Animal Science
Niclas Hogberg, Anna Hessle, Lena Lidfors, Paulius Baltrusis, Edwin Claerebout, Johan Hoglund
Summary: This study investigated the activity and rumination patterns in first-season grazing steers exposed to subclinical infection levels of gastrointestinal nematodes. The results showed that behavioral measurements were affected even at low infection levels but did not affect body weight gain.
Article
Parasitology
G. Suarez, P. Geldhof, J. Borloo, R. Perez-Caballero, D. Robaina, L. Buffoni, P. Alonzo, A. Martinez-Moreno, O. Correa, J. Tort, J. Perez, E. Claerebout
Summary: The research indicated that the dd-Co-ASP protein isolated from the bovine small intestinal parasite could protect cattle from infection, but was not effective against C. curticei infection.
VETERINARY PARASITOLOGY
(2021)
Article
Virology
Bert Vanmechelen, Michelle Merino, Valentijn Vergote, Lies Laenen, Marijn Thijssen, Joan Marti-Carreras, Edwin Claerebout, Piet Maes
Summary: Recent metagenomics studies have identified previously undiscovered RNA viruses in various tick species, with the most prevalent European tick species, Ixodes ricinus, found to host a variety of viruses. Through high-throughput sequencing of tick samples from six different sites in Belgium, several new reoviruses were discovered, including a new Eyach virus strain, potentially indicating genetic reassortment. Screening of over 230 tick pools for selected viruses showed widespread distribution throughout the Belgian tick population, highlighting the importance of tick control measures.
Article
Parasitology
Thomas Geurden, Filip De Keersmaecker, Sofie De Keersmaecker, Edwin Claerebout, Dave M. Leathwick, Martin K. Nielsen, Christian W. Sauermann
Summary: The study evaluated a reduced treatment frequency anthelmintic regimen in horses at two study sites in Belgium over three consecutive summer pasture seasons. It was observed that there was no increase in parasitic disease over the three-year period of the study. Model simulations indicated that a whole-herd treatment regimen with at least 4 macrocyclic lactone treatments annually led to faster resistance development compared to other treatment regimens evaluated.
VETERINARY PARASITOLOGY
(2021)
Article
Agriculture, Dairy & Animal Science
R. Meyermans, S. Janssens, A. Cousse, W. Gorssen, X. Hubin, P. Mayeres, W. Veulemans, E. Claerebout, C. Charlier, N. Buys
Summary: The mutation in the myostatin gene in Belgian Blue cattle is associated with susceptibility to psoroptic mange and an increase in lesion size.
Article
Microbiology
Liana Beld, Hyeim Jung, Christina A. Bulman, Bruce A. Rosa, Peter U. Fischer, James W. Janetka, Sara Lustigman, Judy A. Sakanari, Makedonka Mitreva
Summary: Current treatments for lymphatic filariasis and onchocerciasis are not effective in killing the adult parasitic nematodes. Researchers have found that certain HIV antiretroviral drugs, specifically aspartyl protease inhibitors, show potential in killing adult filarial nematodes. Nelfinavir had the best potency in killing the nematodes, followed by ritonavir and lopinavir. These findings suggest that these drugs could be explored as potential treatments for parasitic nematode infections.
Review
Parasitology
Johannes Charlier, Diana J. Williams, Nadine Ravinet, Edwin Claerebout
Summary: Helminth infections of cattle have significant impacts on livestock production and farm economic efficiency. Subclinical infections, which have hidden impacts on animals, require refined diagnostics to detect and inform farmers about the likely impact of anthelmintic treatment. Recent advances in diagnosing major cattle helminth infections and searching for subclinical infection thresholds can help farmers tailor helminth treatments to specific epidemiological circumstances, thereby limiting anthelmintic resistance and boosting agricultural efficiency and food security.
TRENDS IN PARASITOLOGY
(2023)
Review
Veterinary Sciences
Marieke Brys, Edwin Claerebout, Koen Chiers
Summary: Chronic progressive lymphedema (CPL) in draft horses is a serious condition with severe clinical signs, including progressive swelling of the limbs, dermal fibrosis, and the development of skinfolds and nodules. Despite the severity of the disease, uncertainties regarding its cause and progression remain. This review discusses possible hypotheses for CPL's pathogenesis, summarizes advances in diagnosis and management, and presents a new scoring system for assessing clinical severity.
VETERINARY SCIENCES
(2023)
Review
Agriculture, Dairy & Animal Science
Johannes Charlier, Tong Wang, Sien H. Verschave, Johan Hoeglund, Edwin Claerebout
Summary: Gastrointestinal nematode infections pose a significant threat to the health and well-being of calves in pasture-based rearing systems. The serum pepsinogen assay, although an established tool, is not widely implemented due to its high cost and lack of standardization. This study evaluates the O. ostertagi-Ab ELISA as a cost-effective and robust alternative diagnostic method for first-season grazing calves and suggests that it can replace the serum pepsinogen assay for monitoring purposes at the end of the grazing season.
Article
Veterinary Sciences
Zhenzhen Chen, Edwin Claerebout, Koen Chiers, Mathilde Pas, Bart Pardon, Wouter van Mol, Stijn Casaert, Nathalie De Wilde, Luc Duchateau, Peter Geldhof
Summary: Psoroptic mange is a common disease in livestock caused by Psoroptes ovis, with the Belgian Blue (BB) cattle breed being more susceptible than Holstein-Friesian (HF) cattle. The higher susceptibility of BB cattle may be attributed to a stronger Th2-type immune response leading to more pronounced skin lesions. Anti-inflammatory treatment could potentially serve as an alternative to control the pathology caused by this parasite.
VETERINARY RESEARCH
(2021)
Article
Parasitology
Alizee Hendrickx, Cedric Marsboom, Laura Rinaldi, Hannah Rose Vineer, Maria Elena Morgoglione, Smaro Sotariki, Giuseppe Cringoli, Edwin Claerebout, Guy Hendrickx
Summary: This study evaluated the constraints of using historical data sets to model the spatial distribution of helminth parasites in ruminants, finding that the spatial distribution of input data appears to be more important than the actual sample size. Historical data sets often contain sampling biases and large geographical gaps, and if these biases are not corrected, the outputs of spatial distribution models may only reflect sampling effort.