期刊
PARASITOLOGY
卷 140, 期 1, 页码 46-60出版社
CAMBRIDGE UNIV PRESS
DOI: 10.1017/S0031182012001175
关键词
Sphaerospora; Myxozoa; cyprinid; morphometry; cryptic speciation; ribosomal DNA; molecular identification; blood stages; multi-species infection
类别
资金
- Czech Science Foundation (Center of Excellence grant) [505/12/G112]
- Institute of Parasitology, BC of the ASCR [Z60220518]
- Czech Science Foundation [P506/11/P724]
- Hungarian Scientific Research Fund [OTKA K75873]
Myxozoans are a group of diverse, spore-forming metazoan microparasites bound to aquatic environments. Sphaerospora dykovae (previously S. renicola) causes renal sphaerosporosis and acute swim bladder inflammation (SBI) in juvenile Cyprinus carpio carpio, in central Europe. A morphologically similar species with comparably low pathogenicity, S. angulata has been described from C. c. carpio, Carassius auratus auratus and Carassius gibelio. To clarify uncertainties and ambiguities in taxon identification in these hosts we decided to re-investigate differences in spore morphology using a statistical approach, in combination with SSU and LSU rDNA sequence analyses. We found that developing spores of S. angulata and S. dykovae cannot be distinguished morphologically and designed a duplex PCR assay for the cryptic species that demonstrated S. dykovae is specific to C. c. carpio, whereas S. angulata infects C. a. auratus and C. gibelio. The molecular identification of myxozoan blood stages in common carp and goldfish, which had previously been ascribed to Sphaerospora spp. showed that approximately 75% of blood stages were from non-sphaerosporid coelozoic species infecting these cyprinids and more than 10% were from an alien species, Myxobilatus gasterostei, developing in sticklebacks. We hereby report non-selective myxozoan host invasion and multi-species infections, whose role in SBI still requires clarification.
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