期刊
PARASITOLOGY
卷 137, 期 10, 页码 1515-1526出版社
CAMBRIDGE UNIV PRESS
DOI: 10.1017/S0031182010000326
关键词
Bonamia ostreae; Bonamia exitiosa; Crassostrea gigas; carrier/reservoir; PCR; ISH
类别
资金
- Marine Institute and the Marine RTDI Measure, Productive Sector Operational Programme, National Development Plan
- Xunta de Galicia [PGIDIT05RMA50101PR, PGIDIT-CIMA06/01]
- Ministerio de Ciencia e Innovacion of the Spanish Government
This study investigated the ability of the Pacific oyster, Crassostrea gigas, to act as a carrier or reservoir of the protistan Bonamia ostreae. Studies were carried out independently in Ireland and in Spain. Naive C. gigas were exposed to B. ostreae both in the field and in the laboratory via natural exposure or experimental injection. Naive flat oysters, Ostrea edulis, were placed in tanks with previously exposed C. gigas. Oysters were screened for B. ostreae by examination of ventricular heart smears and by polymerase chain reaction (PCR) screening of tissue samples (gill and/or heart) and shell cavity fluid. PCR-positive oysters were further screened using histology and in situ hybridization (ISH). B. ostreae DNA was detected in the tissues and/or shell cavity fluid of a small number of C. gigas in the field and in the laboratory. B. ostreae-like cells were visualized in the haemocytes of 1 C. gigas and B. ostreae-like cells were observed extracellularly in the connective tissues of 1 other C. gigas. When C. gigas naturally exposed to B. ostreae were held with naive O. edulis, B. ostreae DNA was detected in O. edulis; however, B. ostreae cells were not visualized. In Spain, B. exitiosa DNA was also detected in Pacific oyster tissues. The results of this study have important implications for C. gigas transfers from B. ostreae-endemic areas to uninfected areas and highlight B. ostreae and B. exitiosa's ability to survive extracellularly and in other non-typical hosts.
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