Article
Multidisciplinary Sciences
Tadafumi Yokoyama, Yuko Tasaki, Natsumi Inoue, Naotoshi Sugimoto, Eri Nariai, Sanae Kuramoto, Taizo Wada
Summary: This study developed a RT-LAMP technique for the diagnosis of HPeVs, particularly HPeV3, allowing for rapid and convenient detection of HPeV3 RNA. The technique showed clinical utility in diagnosing newborns and infants with sepsis-like symptoms.
Review
Medicine, General & Internal
Marcin Lenkowski, Kacper Nijakowski, Mariusz Kaczmarek, Anna Surdacka
Summary: The LAMP technique offers advantages in rapid and sensitive detection of multiple pathogens simultaneously, reducing the time needed for identification and assessment of periodontal status, which can be crucial in clinical practice.
JOURNAL OF CLINICAL MEDICINE
(2021)
Article
Biotechnology & Applied Microbiology
Hyeonseek Park, Doo Ri Kim, Areum Shin, Eunjung Jeong, Sohee Son, Jin-Hyun Ahn, So Yoon Ahn, Suk-Joo Choi, Soo-young Oh, Yun Sil Chang, Yae-Jean Kim, Minhee Kang
Summary: Congenital CMV infection is a common cause of hearing loss and neurodevelopmental impairment in newborns. A robust LAMP assay was developed for large-scale screening of newborns, providing higher sensitivity and shorter detection time compared to qPCR. The colorimetric LAMP assay can also be used for point-of-care testing in low-resource environments.
APPLIED MICROBIOLOGY AND BIOTECHNOLOGY
(2023)
Article
Chemistry, Analytical
Patcharapan Suwannin, Duangporn Polpanich, Saovanee Leelayoova, Mathirut Mungthin, Pramuan Tangboriboonrat, Abdelhamid Elaissari, Kulachart Jangpatarapongsa, Toon Ruang-areerate, Tienrat Tangchaikeeree
Summary: The study developed a simple and highly sensitive detection method using LAMP technology and SA-GNPs for visual detection of the 18S rRNA gene of Plasmodium falciparum. This method can complete all steps within 50 minutes, with high specificity and sensitivity, suitable for malaria diagnostics in resource-limited regions.
JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS
(2021)
Article
Medicine, General & Internal
Woong Sik Jang, Da Hye Lim, YoungLan Choe, Hyunseul Jee, Kyung Chul Moon, Chaewon Kim, Minkyeong Choi, In Su Park, Chae Seung Lim
Summary: The developed multiplex malaria Pan/Pf/Pv/IC LAMP assay showed comparable sensitivity (100%) and specificity (100%) with the commercial RealStar((R)) Malaria S&T PCR Kit 1.0 after evaluating 208 clinical samples. These results suggest that the multiplex malaria Pan/Pf/Pv/IC LAMP could be used as a point-of-care molecular diagnostic test for malaria.
Article
Entomology
Yue Zhang, Weisong Li, Massimiliano Virgilio, Marc De Meyer, Zhihong Li
Summary: This study achieved rapid molecular identification of Ceratitis fruit flies using LAMP technology and mitochondrial genes as targets. A loop-mediated isothermal amplification identification technology system was established, which utilized rapid, rough DNA extraction and visual detection. The system demonstrated high sensitivity and simplicity, allowing the entire identification process to be completed within one hour.
JOURNAL OF ECONOMIC ENTOMOLOGY
(2023)
Article
Biophysics
Jayeon Song, Hyo Yong Kim, Soohyun Kim, Yujin Jung, Hyun Gyu Park
Summary: A novel technology, SP-HAMP, is described for target nucleic acid detection under isothermal conditions. By designing a unique hairpin probe, it is possible to detect target DNA with outstanding discrimination capability using only a single primer, without the need for complex multiple primers.
BIOSENSORS & BIOELECTRONICS
(2021)
Article
Chemistry, Analytical
Huan-Huan Zhu, Yuan Li, Li-Xia Wu, Ke-Sheng Wang, Yue Zhang, Qiang-Yuan Fan, Zun-Zhen Ming, Wei-Qin Chen, Wei-Wei Liu
Summary: Loop-mediated isothermal amplification (LAMP) is a promising diagnostic tool for genetic amplification, known for its speed, simplicity, efficiency, and sensitivity. This research introduces an internal heating method for LAMP using magnetic nanoparticles (MNPs) called nano-LAMP, which can detect low concentrations of HPV and has been validated in clinical samples.
Review
Chemistry, Analytical
Jesse M. M. Marangoni, Kenneth K. S. Ng, Arezoo Emadi
Summary: Loop-mediated isothermal amplification (LAMP) is a useful tool for pathogen detection in clinical and environmental samples, but monitoring LAMP can be challenging for point-of-use applications. This review discusses the use of voltammetric sensors for LAMP monitoring and highlights various strategies for detecting DNA amplicons, pyrophosphate, and protons. The review also addresses the hurdles in adapting these sensors for on-site applications.
Article
Chemistry, Analytical
Meng Chen, Qidi He, Yanli Tong, Zuanguang Chen
Summary: This study successfully combined LAMP with RCA to achieve rapid detection and quantification of pathogens. By using a strand displacement strategy in a single-tube reaction, different pathogen target genes were successfully detected, demonstrating the universality and reliability of the method.
SENSORS AND ACTUATORS B-CHEMICAL
(2021)
Article
Parasitology
Xi Chen, Jiaqi Zhang, Maohua Pan, Yucheng Qin, Hui Zhao, Pien Qin, Qi Yang, Xinxin Li, Weilin Zeng, Zheng Xiang, Mengxi Duan, Xiaosong Li, Xun Wang, Dominique Mazier, Yanmei Zhang, Wei Zhao, Benjamin M. Rosenthal, Yaming Huang, Zhaoqing Yang
Summary: The study utilized LAMP technology to design detection methods for Plasmodium spp., P. vivax, and P. ovale, demonstrating high sensitivity and specificity in clinical samples. These novel LAMP assays can provide rapid, reliable, and highly sensitive diagnosis of Plasmodium spp. infections, including those prone to clinical relapse such as P. vivax and P. ovale.
PARASITES & VECTORS
(2021)
Review
Plant Sciences
Sandra V. Gomez-Gutierrez, Stephen B. Goodwin
Summary: LAMP is a molecular technique for detection of plant pathogens, known for its specificity, sensitivity, and ability to conduct reactions at a constant temperature. This review explains the amplification process, visualization techniques, advantages, and disadvantages of LAMP compared to alternative isothermal approaches. It also presents the application of LAMP in detecting fungal pathogens and viruses in wheat, as well as the development of real-time and multiplex LAMP assays.
FRONTIERS IN PLANT SCIENCE
(2022)
Article
Microbiology
Ran Shu, Xianhui Yin, Youhua Long, Jun Yuan, Houyin Zhou
Summary: In this study, a loop-mediated isothermal amplification (LAMP) analysis was developed for the specific detection of Pantoea agglomerans, a primary bacterial disease causing abnormal growth of plum trees and economic losses. The LAMP technology demonstrated high sensitivity and accuracy, making it suitable for early diagnosis of plum bacterial shot-hole disease.
FRONTIERS IN MICROBIOLOGY
(2022)
Article
Multidisciplinary Sciences
Martha A. Clark, Usheer Kanjee, Gabriel W. Rangel, Laura Chery, Anjali Mascarenhas, Edwin Gomes, Pradipsinh K. Rathod, Carlo Brugnara, Marcelo U. Ferreira, Manoj T. Duraisingh
Summary: During Plasmodium intra-erythrocytic development, parasites compromise the structural integrity of host red-blood cells. Researchers found that P. vivax infection destabilizes host reticulocytes, which are less stable than normocytes infected with P. falciparum.
NATURE COMMUNICATIONS
(2021)
Article
Food Science & Technology
T. Mounika, P. S. Girish, M. Shashi Kumar, Aparana Kumari, Sujatha Singh, Nagappa S. Karabasanavar
Summary: The study developed a rapid sheep meat identification technique using DNA extraction and LAMP technology with high specificity and sensitivity. The detection method is simple, rapid, suitable for under-resourced laboratories, and can be used for sheep meat authentication in a short period of time.
JOURNAL OF FOOD SCIENCE AND TECHNOLOGY-MYSORE
(2021)
Article
Infectious Diseases
Fan-Zhen Mao, Yu-Ying Chen, Xiang-Zhen Xu, Bi-Xian Ni, Xiao-Lin Jin, Yang Dai, Jun Cao
Summary: From 1989 to 2019, a multi-intervention integrated deworming approach in Jiangsu Province of China, including mass drug administration, health education, and environmental sanitation improvements, significantly reduced the infection rate of soil-transmitted helminths, demonstrating the effectiveness of comprehensive interventions in sustaining control efforts.
INFECTIOUS DISEASES OF POVERTY
(2021)
Biographical-Item
Parasitology
Pietro Alano, Richard Culleton, Christian Doeing, Louis Miller
Summary: The malaria research community lost a pioneer with the passing of Professor Richard Carter, a brilliant malariologist known for his inquisitive nature and unorthodox way of thinking.
TRENDS IN PARASITOLOGY
(2022)
Article
Infectious Diseases
Sabin S. Nundu, Hiroaki Arima, Shirley Simpson, Ben-Yeddy Abel Chitama, Yannick Bazitama Munyeku, Jean-Jacques Muyembe, Toshihiro Mita, Steve Ahuka, Richard Culleton, Taro Yamamoto
Summary: The study found that deletions of the P. falciparum histidine-rich protein 2/3 genes are uncommon in the surveyed population in Kinshasa, Democratic Republic of Congo, and do not result in diagnostic failure.
Article
Environmental Sciences
Nurul Athirah Naserrudin, Rozita Hod, Mohammad Saffree Jeffree, Kamruddin Ahmed, Richard Culleton, Mohd Rohaizat Hassan
Summary: The review identifies factors such as attitudes, disease misconceptions, perceived threat of disease, lack of motivation, and supernatural or traditional beliefs influencing the exposure of individuals or communities to P. knowlesi malaria. Forestry activities and sleeping outdoors are also identified as important factors.
INTERNATIONAL JOURNAL OF ENVIRONMENTAL RESEARCH AND PUBLIC HEALTH
(2022)
Article
Genetics & Heredity
Wei-ping Liu, Pei-yi Zhang, Sui Xu, Jian-xia Tang, Hua-yun Zhou, Ju-lin Li, Dao-jin Wang, Yuan-yuan Liu, Qiang Fang, Hui Xia, Guo-ding Zhu, Zhi-yong Tao
Summary: This study reports the complete mitochondrial genome of Anopheles anthropophagus for the first time and provides new data for its taxonomic and phylogenetic studies.
MITOCHONDRIAL DNA PART B-RESOURCES
(2022)
Article
Parasitology
Xin Yao, Yujie Cao, Li Lu, Yuanxia Xu, Hao Chen, Chuanqi Liu, Dianyi Chen, Kexue Wang, Jingxiang Xu, Runqi Fang, Hui Xia, Jiangyan Li, Qiang Fang, Zhiyong Tao
Summary: The study found that Plasmodium infection can play an anti-colon cancer role by inhibiting proliferation and promoting mitochondria-mediated apoptosis in colon cancer cells. Plasmodium infection can also disturb mitochondrial biogenesis and mitophagy in colon cancer cells.
PARASITES & VECTORS
(2022)
Article
Microbiology
Yan-Jun Kang, Guoding Zhu, Yang Cheng, Haitao Yang, Jun Cao
Summary: ISVs are considered ancestors to arboviruses. This study characterized the RNA viromes in different tissues of two mosquito species using a metatranscriptomics approach. The results provide important insights into the dynamics of viromes within individual mosquitoes and help understand the evolution, transmission routes, and maintenance modes of mosquito-specific viruses in nature.
MICROBIOLOGY SPECTRUM
(2022)
Review
Biology
Basu Dev Pandey, Mya Myat Ngwe Tun, Kishor Pandey, Shyam Prakash Dumre, Khin Mya Nwe, Yogendra Shah, Richard Culleton, Yuki Takamatsu, Anthony Costello, Kouichi Morita
Summary: This study evaluates the government of Nepal's response to the COVID-19 pandemic and explores ways to prevent COVID-19 and other pandemic diseases in the future. As of May 2022, Nepal has reported a significant number of COVID-19 cases and deaths. To prevent the spread of the virus, the government has implemented various preventive and control measures.
Article
Public, Environmental & Occupational Health
Zhi-yong Tao, Pei-yi Zhang, Lu Zhang, Chun-cao Li, Rui Hu, Han-wu Zhu, Bei Zhou, Kai Wu, Ling-xu Li, Da-wei Yao, Yu-jie Cao, Dao-jin Wang, Chen-chen Zheng, Run-qi Fang, Xiu-min Hua, Yi-xuan Ni, Xiao-xia Jin, Hui Xia, Qiang Fang
Summary: The study demonstrates that DBS-based PCR is a cost-effective and feasible method for detecting blood pathogens, providing valuable reference for screening the cost-efficacy combination of filter paper and PCR kit.
JOURNAL OF TROPICAL MEDICINE
(2022)
Article
Environmental Sciences
Karen Zhang, Di Liang, Donglan Zhang, Jun Cao, Jiayan Huang
Summary: This study highlights a significant gap in Senegal between high rates of antenatal care attendance and low uptake of intermittent preventive treatment in pregnancy with sulfadoxine-pyrimethamine (IPTp-SP). Findings suggest that some antenatal care facilities in Senegal do not always adhere to national guidelines in providing IPTp-SP, emphasizing the need for further provider training and patient engagement.
INTERNATIONAL JOURNAL OF ENVIRONMENTAL RESEARCH AND PUBLIC HEALTH
(2022)
Article
Microbiology
Yougui Yang, Xin Ding, Fuzhong Chen, Xiaomin Wu, Yuying Chen, Qiang Zhang, Jun Cao, Junhong Wang, Yang Dai
Summary: Atherosclerosis is a major cause of death and disability worldwide, involving inflammation. Studies have shown that intervention with helminth infections or derived products can reduce the onset and progression of atherosclerosis, possibly through modulation of the immune response and anti-inflammatory cytokines.
Review
Parasitology
Richard Culleton, Arnab Pain, Georges Snounou
Summary: Plasmodium malariae is a neglected malaria parasite, with less research conducted compared to its more notorious cousins, P. falciparum and P. vivax. However, there has been increasing interest in this parasite due to improved detection techniques revealing a wider prevalence and high numbers of chronic, asymptomatic infections, particularly in Africa.
TRENDS IN PARASITOLOGY
(2023)
Article
Environmental Sciences
Nurul Athirah Naserrudin, Richard Culleton, Pauline Yong Pau Lin, Sara Elizabeth Baumann, Rozita Hod, Mohammad Saffree Jeffree, Kamruddin Ahmed, Mohd Rohaizat Hassan
Summary: This study explores the importance of gatekeepers in conducting research among rural communities, their perspectives on the challenges encountered in avoiding malaria infection, and their views on participatory research. The study highlights various challenges in malaria control, including social norms, lifestyles, socioeconomic factors, environmental factors, and limitations of basic resources.
INTERNATIONAL JOURNAL OF ENVIRONMENTAL RESEARCH AND PUBLIC HEALTH
(2022)
Article
Microbiology
Chun-cao Li, Rui Hu, Xiu-min Hua, Yi-xuan Ni, Lu Ge, Lu Zhang, Wen Yu, Ni-xin Hao, Hui Xia, Qiang Fang, Zhi-yong Tao
Summary: Antibiotic resistance screening is crucial in recombinant plasmid construction. The dfrB10 gene has been identified as a promising marker for screening antibiotic drug resistance in plasmid construction. This study successfully replaced the original resistance genes of different plasmids with dfrB10, constructing a series of plasmids with trimethoprim (TMP) resistance. The function of the plasmids remained unaffected, and a miniature tool plasmid, pTMi, with TMP resistance was also constructed.
MICROBIOLOGY SPECTRUM
(2023)
Article
Microbiology
Medhavi Dhakal, Tulsi Ram Gompo, Prakash Devkota, Sharmila Chapagain Kafle, Janak Raj Subedi, Haiyan Gong, Hiroaki Arima, Richard Culleton, Masahito Asada, Kishor Pandey
Summary: In Nepal, TBPPs infecting cattle, including Babesia and Theileria, were successfully identified using conventional nested PCR and sequence analysis. This study provides a starting point for TBPP surveillance and prevention programs in Nepal.