Article
Zoology
Laura Alejandra Arriola-Mosqueda, Yadira Jimenez-Lara, Eugenia del Carmen Prieto-Avella, Carlos Ricardo Cruz-Vazquez, Roberto Montesinos-Matias, Mauricio Valencia-Posadas, Ana Martha Cruz-Avalos, Jaime Molina-Ochoa, Cesar Andres Angel-Sahagun
Summary: This research evaluated the virulence of Metarhizium anisopliae on the eggs and larvae of Rhipicephalus sanguineus, as well as the germination, radial growth, and LC50 values of different strains. The study found that M. anisopliae is effective for controlling R. sanguineus, but there are differences in germination, radial growth, and virulence among different strains. Additionally, biological characteristics were not correlated with the fungal virulence against R. sanguineus larvae.
PAKISTAN JOURNAL OF ZOOLOGY
(2021)
Article
Cell Biology
Diego Carlos Andrade Pereira, Rafael Goncalves Teixeira-Neto, Valeriana Valadares Lopes, Heber Paulino Pena, Gustavo Fontes Paz, Carlos Henrique Xavier Custodio, Vinicius Silva Belo, Antonio Augusto da Fonseca Junior, Eduardo Sergio da Silva
Summary: In this study, qPCR and ddPCR were used to quantify the parasite load in spleen samples from dogs infected with Leishmania (L.) infantum. The results showed 100% agreement between the two methods. These tools are important for the detection of L. infantum in dogs and humans, and ddPCR, as a new tool, can provide more in-depth information about parasitic loads and the pathogenesis of leishmaniasis.
MOLECULAR AND CELLULAR BIOCHEMISTRY
(2023)
Article
Parasitology
J. R. Gutierrez-Wong, J. A. Rosado-Aguilar, R. I. Rodriguez-Vivas
Summary: This study evaluated the acaricidal efficacy of plumbagin on larvae of R. microplus and R. sanguineus resistant to conventional acaricides. The results showed that plumbagin had high acaricidal efficacy against both tick species, with R. microplus larvae being more susceptible than R. sanguineus.
EXPERIMENTAL PARASITOLOGY
(2023)
Article
Chemistry, Medicinal
Thibault Joseph William Jacques Dit Lapierre, Mariza Gabriela Faleiro de Moura Lodi Cruz, Nicolas Peterson Ferreira Brito, Daniela de Melo Resende, Felipe de Oliveira Souza, Eduardo Jorge Pilau, Meryck Felipe Brito da Silva, Bruno Junior Neves, Silvane Maria Fonseca Murta, Celso de Oliveira Rezende Junior
Summary: An early hit-to-lead optimization study was performed on a novel pyrazinylpiperazine series against L. infantum and L. braziliensis. The study focused on the benzoyl fragment of a hit compound (4), and deletion of the meta-Cl led to the synthesis of a para-hydroxyl derivative (12). Further optimization involving disubstituted benzoyl fragments and the hydroxyl substituent of (12) resulted in the identification of an ortho, meta-dihydroxyl derivative (46) as an early lead compound with increased anti-leishmanial potency. In silico ADMET predictions revealed satisfactory profiles for these compounds, indicating potential for further lead optimization.
EUROPEAN JOURNAL OF MEDICINAL CHEMISTRY
(2023)
Article
Infectious Diseases
Kosta Y. Mumcuoglu, Agustin Estrada-Pena, Evelina L. Tarragona, Patrick S. Sebastian, Alberto A. Guglielmone, Santiago Nava
Summary: This study reestablished Rhipicephalus secundus as a valid tick name within the Rhipicephalus sanguineus group and provided a morphological re-description and mitochondrial DNA sequence analysis to determine its classification. Morphological features and the shape of the spiracular plate were used to differentiate R. secundus from other related species such as R. turanicus and R. sanguineus.
TICKS AND TICK-BORNE DISEASES
(2022)
Article
Immunology
Hugo O. Valdivia, Bruno M. Roatt, Rodrigo de Paula Baptista, Jennifer Ottino, Anderson Coqueiro-dos-Santos, Mandy J. Sanders, Alexandre B. Reis, James A. Cotton, Daniella C. Bartholomeu
Summary: Visceral leishmaniasis, an important global health problem, is particularly prevalent in Brazil. This study reveals a turnover of parasite populations in a re-emergent focus in Brazil, which presents new challenges for disease control efforts.
FRONTIERS IN CELLULAR AND INFECTION MICROBIOLOGY
(2022)
Article
Pharmacology & Pharmacy
Patricia de A. Machado, Pollyanna S. Gomes, Monique P. D. Carneiro, Victor Midlej, Elaine S. Coimbra, Herbert L. de Matos Guedes
Summary: This study demonstrates the importance of serine proteases in Leishmania and evaluates the antileishmanial effects of a serine protease inhibitor, TPCK. TPCK shows selectivity for the parasite and has significant activity against both promastigote and intracellular amastigote forms. The treated parasites exhibit various alterations, suggesting potential mechanisms contributing to their death. In vivo studies show that TPCK reduces lesion size and parasite loads, highlighting its potential as a therapeutic target for leishmaniasis.
Article
Immunology
Thais T. O. Santos, Amanda S. Machado, Fernanda F. Ramos, Joao A. Oliveira-da-Silva, Daniela P. Lage, Grasiele S. Tavares, Debora V. C. Mendonca, Mariana S. Cardoso, Williane F. Siqueira, Vivian T. Martins, Fernanda Ludolf, Thiago A. R. Reis, Livia M. Carvalho, Camila S. Freitas, Raquel S. Bandeira, Alessandra M. Silva, Jamil S. Oliveira, Ricardo L. F. Moreira, Ricardo T. Fujiwara, Bruno M. Roatt, Miguel A. Chavez-Fumagalli, Maria Humbert, Antonio L. Teixeira, Eduardo A. F. Coelho
Summary: The study demonstrates that the Leishmania eukaryotic Elongation Factor-1 beta (EF1b) protein can be a promising vaccine candidate for preventing L. infantum infection. Vaccination with EF1b induces specific Th1-type immune response, reduces parasite load, and provides stronger protection against infection.
MICROBIAL PATHOGENESIS
(2021)
Article
Entomology
R. A. Castro, O. P. Neto, A. L. S. Mendes, L. H. Moreira
Summary: Natural compounds with acaricidal activity, such as ozone, show potential in controlling ticks and minimizing economic loss in cattle herds.
EXPERIMENTAL AND APPLIED ACAROLOGY
(2023)
Article
Biochemistry & Molecular Biology
Mayara Castro de Morais, Gisele Alves Medeiros, Fernanda Silva Almeida, Juliana da Camara Rocha, Yunierkis Perez-Castillo, Tatjana de Souza Lima Keesen, Damiao Pergentino de Sousa
Summary: In this study, a new potential therapeutic drug, N-(4-isopropylbenzyl)cinnamamide, was reported to have strong antimicrobial activity against Leishmania, with higher selectivity index compared to common antimicrobial drugs.
Article
Microbiology
Wittawat Wechtaisong, Sarah I. Bonnet, Bruno B. Chomel, Yi-Yang Lien, Shih-Te Chuang, Yi-Lun Tsai
Summary: The study found that Bartonella henselae can be detected in the midguts, salivary glands, and carcasses of semi-engorged adults, as well as in pooled tick feces, but not in eggs or larvae derived from infected females. While B. henselae DNA was detected in the blood sample during larval feeding, no viable bacteria were isolated through culture. This suggests the possibility of transovarial transmission of B. henselae in Rhipicephalus sanguineus sensu lato ticks.
Article
Veterinary Sciences
Julia Ramos Sampaio, Rodrigo Pedro Soares, Thiago Doria Barral, Gabriela Porfirio Passos, Maisa Santos Fonseca, Roberto Meyer, Stella Maria Barrouin-Melo, Ricardo Wagner Portela
Summary: The study found that GIPLs could serve as an ELISA target for dogs infected with Leishmania infantum, but with slightly lower sensitivity and specificity compared to LPG. While GIPLs performed similarly to LPG for subclinically infected dogs, cross-reactions with other protozoa and a lower sensitivity limit its use for immunodiagnostic testing.
FRONTIERS IN VETERINARY SCIENCE
(2021)
Article
Immunology
Graziele Quintela-Carvalho, Astrid Madeleine Calero Goicochea, Vanessa Mancur-Santos, Sayonara de Melo Viana, Yasmin da Silva Luz, Beatriz Rocha Simoes Dias, Milena Lazaro-Souza, Martha Suarez, Camila Indiani de Oliveira, Elvira M. Saraiva, Claudia Brodskyn, Patricia T. Veras, Juliana P. B. de Menezes, Bruno B. Andrade, Jonilson Berlink Lima, Albert Descoteaux, Valeria M. Borges
Summary: Lipophosphoglycan (LPG) may play a critical role in promoting Leishmania survival in human neutrophils through cellular activation and generation of free radicals, thereby influencing the process of infection.
FRONTIERS IN CELLULAR AND INFECTION MICROBIOLOGY
(2022)
Article
Parasitology
Tao Jin, Claudia Husseneder, Lane Foil
Summary: This study utilized DNA barcoding to identify Culicoides larvae collected from tree holes, revealing the species distribution of Culicoides vectors and their larval habitats. By matching adult sequences with larval sequences, 16 larvae were successfully assigned to specific species.
PARASITES & VECTORS
(2022)
Article
Physiology
Liping An, Biswajit Bhowmick, Dejuan Liang, Penghui Suo, Chenghong Liao, Jianguo Zhao, Qian Han
Summary: This study investigated the impact of starvation stress on the microbiome of Rhipicephalus sanguineus ticks and found that starvation stress significantly altered the microbial composition. The dominant symbiotic bacteria, Coxiella spp., decreased with prolonged starvation stress. Additionally, the study demonstrated that ticks can tolerate starvation for up to 6 months. Furthermore, the removal of most symbiotic bacteria had irreversible effects on the blood-sucking rate, weight, fecundity, and feeding period of the ticks.
FRONTIERS IN PHYSIOLOGY
(2022)
Article
Parasitology
E. Napoli, R. Panarese, F. La Russa, I Cambera, J. A. Mendoza-Roldan, D. Otranto, E. Brianti
Summary: The potential involvement of Culicoides spp. in the transmission of Dirofilaria spp. as well as the blood-meal source was investigated. The detection of D. immitis and D. repens DNA in Culicoides midges suggests their potential role in the epidemiology of these filarial nematodes.
Article
Public, Environmental & Occupational Health
Aya Attia Koraney Zarea, Marcos Antonio Bezerra-Santos, Viet-Linh Nguyen, Vito Colella, Filipe Dantas-Torres, Lenaig Halos, Frederic Beugnet, Maria Tempesta, Domenico Otranto, Grazia Greco
Summary: This study detected Bartonella spp. and haemoplasmas in cats, dogs, and their fleas from East and Southeast Asia. The dominant flea species infesting cats and dogs was Ctenocephalides felis, which played a key role in Bartonella spp. transmission. Cats had a higher risk of infection with both Bartonella spp. and haemoplasmas compared to dogs. Fleas were not found to be involved in the transmission of haemoplasmas.
ZOONOSES AND PUBLIC HEALTH
(2022)
Review
Infectious Diseases
Jairo Alfonso Mendoza-Roldan, Jan Votypka, Claudio Bandi, Sara Epis, David Modry, Lucie Ticha, Petr Volf, Domenico Otranto
Summary: Leishmaniasis is a neglected tropical parasitic disease transmitted by sand flies. L. tarentolae, previously considered non-pathogenic to mammals, has shown transient infectivity to them. Recent molecular diagnostics and serological positivity to L. tarentolae in humans and dogs have sparked interest in the interactions between mammals, reptiles, and Leishmania infantum, the main cause of leishmaniasis.
TRANSBOUNDARY AND EMERGING DISEASES
(2022)
Editorial Material
Parasitology
Filipe Dantas-Torres, Domenico Otranto
TRENDS IN PARASITOLOGY
(2022)
Article
Veterinary Sciences
Warley Vieira de Freitas Paula, Lucianne Cardoso Neves, Luiza Gabriella Ferreira de Paula, Maria Carolina de Azevedo Serpa, Fabricio Peixoto de Oliveira, Filipe Dantas-Torres, Sebastian Munoz-Leal, Marcelo Bahia Labruna, Felipe da Silva Krawczak
Summary: In this study, the first molecular detection of B. theileri subclinical infection in a cow in Brazil is described. The detection was confirmed using PCR, and the implications of this finding for cattle health management in the region are discussed.
VETERINARY RESEARCH COMMUNICATIONS
(2023)
Letter
Infectious Diseases
Marcos Antonio Bezerra-Santos, Filipe Dantas-Torres, Domenico Otranto
INTERNATIONAL JOURNAL OF INFECTIOUS DISEASES
(2022)
Article
Microbiology
Andrea Grassi, Matteo Gambini, Marianna Pantoli, Simona Toscano, Anna Albertetti, Deborah Maria Del Frassino, Iniobong Chukwuebuka Ikenna Ugochukwu, Orazio Romeo, Domenico Otranto, Claudia Cafarchia
Summary: This study described a case of lethal Cladosporium allicinum infection in a captive bullfrog in Europe. The infection was confirmed by histology, cytology, and molecular methods. Despite antifungal treatment, the frog died, highlighting the poor prognosis of Cladosporium infections in animals.
Article
Public, Environmental & Occupational Health
Anna Morea, Angela Stufano, Giovanni Sgroi, Leonarda De Benedictis, Marcos Antonio Bezerra-Santos, Jairo Alfonso Mendoza-Roldan, Vincenzo Veneziano, Domenico Otranto, Piero Lovreglio, Roberta Iatta
Summary: This study aimed to assess the seroprevalence of L. infantum in different groups of outdoor workers and identified veterinarians as having the highest seroprevalence. It highlights the importance of increased occupational health surveillance and collaboration among different experts to prevent the spread of this disease.
ZOONOSES AND PUBLIC HEALTH
(2023)
Review
Microbiology
Sina Mohtasebi, Alireza Sazmand, Salman Zafari, Guilherme G. G. Verocai, Domenico Otranto
Summary: This report summarizes the scientific information available from 1980 to 2022 on the occurrence of lungworms in non-ruminant mammals and humans in Iran. A total of 10 lungworm species belonging to seven genera were reported. The prevalence of lungworm infection varied among different animal species. Rating: 8/10.
Review
Microbiology
Rafael Antonio Nascimento Ramos, Lucia Oliveira de Macedo, Marcos Antonio Bezerra-Santos, Gilcia Aparecida de Carvalho, Guilherme Gomes Verocai, Domenico Otranto
Summary: Species of Ixodiphagus are parasitoid wasps that develop inside ticks. Adult female wasps lay eggs inside ticks, and the larvae feed on the internal contents before emerging as adult wasps. Ixodiphagus has been reported as a parasitoid of multiple tick species. The most studied species, Ixodiphagus hookeri, has shown potential for reducing tick populations, although control attempts have faced challenges.
Review
Parasitology
Marcos Antonio Bezerra-Santos, Filipe Dantas-Torres, Jairo Alfonso Mendoza-Roldan, R. C. Andrew Thompson, David Modry, Domenico Otranto
Summary: Invasive wild mammals pose a major threat to native ecosystems and can act as spreaders of zoonotic parasites. Europe has the highest number of zoonotic parasites associated with invasive wild mammals.
TRENDS IN PARASITOLOGY
(2023)
Article
Infectious Diseases
Jairo Alfonso Mendoza-Roldan, Viviane Noll Louzada-Flores, Nouha Lekouch, Intissar Khouchfi, Giada Annoscia, Andrea Zatelli, Frederic Beugnet, Julia Walochnik, Domenico Otranto
Summary: This study investigated the presence of parasites and pathogens in the blood and feces of reptiles sold in the markets of Marrakech in order to assess the risk of zoonotic transmission. The results showed that 28.9% of reptiles carried at least one pathogen, including Anaplasma and Rickettsia. In addition, some potentially zoonotic bacteria, such as Proteus vulgaris and Pseudomonas aeruginosa, were found. The study highlights the potential role of reptiles in the transmission of pathogens to humans in the markets of Marrakech.
PLOS NEGLECTED TROPICAL DISEASES
(2023)
Article
Infectious Diseases
Ilaria Varotto-Boccazzi, Sara Epis, Giulia Maria Cattaneo, Noemi Guerrini, Alessandro Manenti, Diego Rubolini, Paolo Gabrieli, Domenico Otranto, Gianvincenzo Zuccotti, Emanuele Montomoli, Claudio Bandi
Summary: The mucosal immune system is crucial for combatting infections and can serve as the first line of defense against various pathogens. Mucosal vaccination has shown promise in protecting against infections acquired through ingestion, inhalation, or the urogenital system, and can also induce systemic immune responses. This study investigated rectal administration of Leishmania parasites as a potential method for generating anti-Leishmania immunity, demonstrating a specific antibody response and a shift towards a Th1-biased immune response. These findings suggest the development of novel vaccines based on rectal immunization against Leishmania parasites should be further explored.
TROPICAL MEDICINE AND INFECTIOUS DISEASE
(2023)
Article
Parasitology
Samuel Ricarte de Aquino, Lucyo Flavio Bezerra Diniz, Savio Luiz Pereira Nunes, Roberta Lane de Oliveira Silva, Gisele Veneroni Gouveia, Joao Jose Simoni Gouveia, Kamila Gaudencio da Silva Sales, Filipe Dantas-Torres, Rodrigo Feliciano do Carmo
Summary: This study investigated the use of kDNA-based qPCR for monitoring parasite load in VL patients after treatment. The results showed a significant reduction in parasite load after treatment, although one patient experienced an increase in parasite load and relapse. These findings demonstrate the utility of kDNA-based qPCR for post-treatment follow-up in VL cases.
REVISTA DA SOCIEDADE BRASILEIRA DE MEDICINA TROPICAL
(2023)