期刊
PARASITE IMMUNOLOGY
卷 33, 期 3, 页码 170-180出版社
WILEY
DOI: 10.1111/j.1365-3024.2010.01268.x
关键词
leishmania; protozoan parasites; T-cell activation; TCR diversity
资金
- National Institutes of Health [AI043003]
P>CD4+ T cells play a critical role in determining the disease outcome in murine cutaneous leishmaniasis, and selective usage of T-cell receptor (TCR) is implied in promoting Leishmania major infection. However, little information is available on TCR usage in Leishmania-specific, IFN-gamma-producing CD4+ T cells. In this study, we investigated the TCR diversity and activation of CD4+ T cells in a nonhealing model associated with L. amazonensis (La) infection and a self-healing model associated with L. braziliensis (Lb) infection. While marked expansion in the absolute number of several subsets was observed in Lb-infected mice, the percentages of TCR V beta+ CD4+-cell subsets were comparable in draining LN- and lesion-derived T cells in two infection models. We found that multiple TCR V beta CD4+T cells contributed collectively and comparably to IFN-gamma production and that the overall levels of IFN-gamma production positively correlated with the control of Lb infection. Moreover, pre-infection with Lb parasites provided cross-protection against secondary La infection, owing to an enhanced magnitude of T-cell activation and IFN-gamma production. Collectively, this study suggests that the magnitude of CD4+ T-cell activation, rather than the TCR diversity, is the major determining factor for the outcome of Leishmania infection.
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