Article
Microbiology
Xue-Lian Luo, Xiu-Dan Zhang, Bei-Jie Li, Tian Qin, Zhi-Jie Cao, Qian-Jin Fan, Jing Yang, Dong Jin, Shan Lu, Ya-Yun Zheng, Xue-Fang Xu, Ji Pu, Jianguo Xu
Summary: Lassa virus is an important human pathogen causing hemorrhagic fever in West Africa, with an increasing risk of imported cases to other countries due to increased traveling. The high nucleotide diversity of the virus strains complicates the development of diagnostic assays. The GPC RT-PCR/2007 assay and the Mabsky kit showed good sensitivity and specificity for detecting most LASV strains.
MICROBIOLOGY SPECTRUM
(2023)
Article
Infectious Diseases
Georgi Tomov, Nikola Stamenov, Deyan Neychev, Kiril Atliev
Summary: This study aimed to examine the possible association between tongue piercing and Candida spp. infection in healthy individuals. The results suggest that the main risk factors for tongue colonization with Candida spp. are gender, smoking, and improper hygiene, while tongue piercing is not a risk factor for colonization of Candida spp.
Article
Chemistry, Multidisciplinary
Byoung-Hoon Kang, Kyung-Won Jang, Eun-Sil Yu, Hamin Na, Yun-Jae Lee, Woong-Yeol Ko, NamHo Bae, Donggee Rho, Ki -Hun Jeong
Summary: This study presents a rapid and compact real-time RT-PCR system for decentralized molecular diagnostics. It utilizes plasmonic nucleic acid amplification and quantification, and features an ultrafast plasmonic thermocycler, a disposable plastic-on-metal cartridge, and a compact fluorescence microscope. The system successfully enables 10-minute diagnosis of COVID-19 with high amplification efficiency, classification accuracy, and total percent agreement for clinical diagnostic tests.
Article
Chemistry, Multidisciplinary
Byoung-Hoon Kang, Kyung-Won Jang, Eun-Sil Yu, Hamin Na, Yun-Jae Lee, Woong-Yeol Ko, NamHo Bae, Donggee Rho, Ki-Hun Jeong
Summary: This study presents an ultrafast plasmonic nucleic acid amplification and real-time quantification system for decentralized molecular diagnostics. The system features ultrafast photothermal cycling, rapid heat transfer, and efficient PCR quantification, along with high-contrast fluorescence microscopic imaging. It enables decentralized point-of-care molecular diagnostic testing in primary care and developing countries.
Article
Immunology
Danna Zhou, Shuangshuang Wang, Keli Yang, Xue Liu, Wei Liu, Rui Guo, Wan Liang, Fangyan Yuan, Zewen Liu, Ting Gao, Yong-xiang Tian
Summary: A new real-time nucleic acid sequence-based amplification (RT-NASBA) method was developed for rapid detection of Japanese encephalitis virus (JEV), showing high specificity and sensitivity.
MICROBIAL PATHOGENESIS
(2021)
Article
Immunology
Gwynngelle A. Borillo, Ron M. Kagan, Elizabeth M. Marlowe
Summary: This study demonstrates that multiplexed real-time RT-PCR genotyping has strong agreement with whole-genome sequencing (WGS) and may provide additional SARS-CoV-2 variant screening capabilities when WGS is unavailable or cost-prohibitive. RT-PCR genotyping assays can also supplement existing sequencing efforts, providing rapid results at or near the time of diagnosis to guide patient management.
FRONTIERS IN CELLULAR AND INFECTION MICROBIOLOGY
(2022)
Article
Food Science & Technology
Wajeehah Wajahat, Z. R. Azaz Ahmad Azad, Sadaf Nazir, Gazia Nasir
Summary: The study evaluated the use of RT-PCR to detect adulteration of camel milk, successfully amplifying camel specific primers. The results indicated positive amplification for camel milk and its products, demonstrating the effectiveness and practicality of this method for identification.
JOURNAL OF FOOD SCIENCE AND TECHNOLOGY-MYSORE
(2022)
Article
Biochemical Research Methods
Wen Zhang, Tao Pan
Summary: In this study, a RT-PCR and gel electrophoresis based method called CLAP was developed to sensitively and quantitatively assess Psi modification in mRNA/lncRNA. The method visualizes both Psi-modified and unmodified target sites and validates high throughput sequencing results.
Article
Endocrinology & Metabolism
Zekun Lyu, Min Zhao, Patricio Atanes, Shanta Jean Persaud
Summary: Under conditions of obesity, the expression of non-olfactory GPCR mRNAs in human islets showed significant alterations, with upregulation of G alpha s- and G alpha q-coupled receptors. This opens up possibilities for exploring novel therapeutic candidates for regulating glucose homeostasis through potentiation of insulin secretion and/or beta-cell mass.
Article
Microbiology
David J. Alouani, Roshani R. P. Rajapaksha, Mehul Jani, Daniel D. Rhoads, Navid Sadri
Summary: This study introduces a novel deep learning approach to interpret RT-PCR data, with the developed model serving as a quality assurance tool for real-time monitoring of result interpretation, applicable to pathogen detection such as SARS-CoV-2.
JOURNAL OF CLINICAL MICROBIOLOGY
(2021)
Article
Biochemistry & Molecular Biology
Peter Braun, Martin Duy-Thanh Nguyen, Mathias C. Walter, Gregor Grass
Summary: A real-time PCR assay targeting a specific single nucleotide polymorphism in Bacillus anthracis genomes was validated, showing high specificity, linearity, and sensitivity. Adapting the assay for reverse transcription PCR on 16S rRNA transcripts significantly increased sensitivity compared to DNA-targeting assays, offering great potential for molecular diagnostics.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Public, Environmental & Occupational Health
V Caramello, A. Macciotta, A. De Salve, A. Mussa, A. M. De Leo, F. Bar, D. Panno, F. Nota, C. Y. G. Ling, F. Solitro, F. Ricceri, C. Sacerdote, A. Boccuzzi
Summary: This study aimed to compare patients who initially tested negative for COVID-19 with those who tested positive in terms of clinical presentation, laboratory results, imaging findings, and prognosis. Results showed that patients with negative initial tests tended to have more comorbidities such as heart disease, immunosuppression, and diabetes, and imaging was useful in identifying them. True positive patients had a higher risk of serious complications.
Article
Virology
Brigitte Sigrist, Jessica Geers, Sarah Albini, Dennis Rubbenstroth, Nina Wolfrum
Summary: Avian bornaviruses were first identified as causative agents of a disease in parrots in 2008. Currently, detection of these viruses is primarily done through RT-PCR, but existing methods have limitations in specificity and breadth of detection. A new multiplex real-time RT-PCR assay has been developed to overcome these limitations and simultaneously detect and differentiate avian bornaviruses.
Article
Plant Sciences
Scott D. Anderson, Cynthia A. Gleason
Summary: Root-knot nematodes, especially Meloidogyne chitwoodi, pose a significant threat to potato production in the Northwestern region of the USA. Recently, two unfamiliar Meloidogyne species, M. fallax and M. minor, were discovered on golf turfgrasses, raising concerns about the potential infestation of potato fields. To address this issue, a molecular beacon real-time PCR assay was developed to accurately detect and differentiate M. chitwoodi, M. fallax, and M. minor from nematode extracts.
FRONTIERS IN PLANT SCIENCE
(2023)
Article
Biochemical Research Methods
Zeinab Alsaheli, Ali Abdallah, Ornella Incerti, Ahmad Shalaby, Sahar Youssef, Michele Digiaro, Toufic Elbeaino
Summary: Singleplex and multiplex real-time RT-PCR assays were developed to detect seven fig-infecting viruses, and a large-scale survey in Egypt showed a high prevalence of mixed infections, with FMV being the most commonly observed virus.
JOURNAL OF VIROLOGICAL METHODS
(2021)
Article
Cell Biology
Hong-Tu Li, Fang-Xu Jiang, Ping Shi, Tao Zhang, Xiao-Yu Liu, Xue-Wen Lin, Zhong-Yan San, Xi-Ning Pang
IN VITRO CELLULAR & DEVELOPMENTAL BIOLOGY-ANIMAL
(2017)
Article
Cell Biology
Younan Chen, Rodrigo Carlessi, Nikita Walz, Vinicius Fernandes Cruzat, Kevin Keane, Abraham N. John, Fang-Xu Jiang, Revathy Carnagarin, Crispin R. Dass, Philip Newsholme
MOLECULAR AND CELLULAR ENDOCRINOLOGY
(2016)
Article
Endocrinology & Metabolism
A. Neelankal John, G. Morahan, F. -X. Jiang
JOURNAL OF NEUROENDOCRINOLOGY
(2017)
Article
Cell & Tissue Engineering
Fang-Xu Jiang, Kevin Li, Michael Archer, Munish Mehta, Emma Jamieson, Adrian Charles, Jan E. Dickinson, Masahito Matsumoto, Grant Morahan
Article
Cell & Tissue Engineering
Fang-Xu Jiang, Leonard C. Harrison
STEM CELLS AND DEVELOPMENT
(2017)
Article
Endocrinology & Metabolism
A. Neelankal John, Z. Iqbal, S. Colley, G. Morahan, M. Makishima, F. -X. Jiang
DIABETES & METABOLISM
(2018)
Article
Endocrinology & Metabolism
Abraham Neelankal John, Ramesh Ram, Fang-Xu Jiang
ENDOCRINE PATHOLOGY
(2018)
Review
Endocrinology & Metabolism
Abraham Neelankal John, Fang-Xu Jiang
JOURNAL OF DIABETES AND ITS COMPLICATIONS
(2018)
Article
Rheumatology
X. Ma, G. Chen, J. Wang, J. Xu, F. Zhao, M. Hu, Z. Xu, B. Yang, J. Guo, S. Sun, M. Liu
SCANDINAVIAN JOURNAL OF RHEUMATOLOGY
(2019)
Article
Biochemistry & Molecular Biology
Hongtu Li, Abraham Neelankal John, Takahiro Nagatake, Yoko Hamazaki, Fang-Xu Jiang
Review
Cell & Tissue Engineering
Zi-Yi Sun, Ting-Yan Yu, Fang-Xu Jiang, Wei Wang
Summary: Type 1 diabetes mellitus (T1DM) is a chronic autoimmune disease that involves the destruction of pancreatic islet beta cells and results in insulin insufficiency. Although islet transplantation therapy has shown potential for restoring glucose homeostasis in severe T1DM patients, it is limited by factors such as donor shortage and cell loss. Stem cell therapy, specifically differentiating beta-like cells from human pluripotent stem cells (hPSCs), has emerged as a promising alternative, but challenges remain in obtaining fully mature and functional beta-like cells for transplantation. Various transcription factors play crucial roles in the differentiation of hPSCs into mature human islet beta cells, and deficiencies in key factors may hinder successful differentiation and islet development in vivo. Efforts are ongoing to address the current obstacles in stem cell therapy for diabetes through understanding beta cell maturation both in vivo and in vitro.
WORLD JOURNAL OF STEM CELLS
(2021)
Review
Biochemistry & Molecular Biology
Miao Zhang, Jianmin Guo, Lifei Liu, Mei Huang, Yuexuan Li, Samuel Bennett, JiaKe Xu, Jun Zou
Summary: NEAT1, a long non-coding RNA highly expressed in various organs, is involved in paraspeckle formation and gene expression, making it a potential biomarker for treating diseases. Despite its potential, the mechanisms and downstream effects of abnormal NEAT1 expression in diseases remain unclear.
BIOCHEMICAL GENETICS
(2022)
Review
Cell & Tissue Engineering
Lu Yang, Zhu-Meng Hu, Fang-Xu Jiang, Wei Wang
Summary: This review presents the current research status and progress of stem cell therapy for insulin-dependent diabetes, highlighting the remaining challenges and issues.
WORLD JOURNAL OF STEM CELLS
(2022)
Review
Cell & Tissue Engineering
Helen Jiang, Fang-Xu Jiang
Summary: Insulin remains the main treatment for type 1 diabetes, but it is not a cure. Stem cell-derived beta cells show potential for cell replacement therapy, but more research is needed to fully understand their nature and address technical and ethical challenges.
WORLD JOURNAL OF STEM CELLS
(2023)
Review
Endocrinology & Metabolism
Fang-Xu Jiang, Grant Morahan
WORLD JOURNAL OF DIABETES
(2016)