Article
Neurosciences
Saad B. Hannan, Reka Penzinger, Ginte Mickute, Trevor G. Smart
Summary: GABA receptors, particularly GABABRs, are important therapeutic targets for neurodevelopmental and neuropsychiatric disorders. However, a widely used positive allosteric modulator, CGP7930, was found to have effects on both GABABRs and GABAARs, as well as GIRK channels, making it unsuitable as a specific GABABR PAM.
Article
Biochemistry & Molecular Biology
Arsalan Yousuf, Xiaosa Wu, Anuja R. Bony, Mahsa Sadeghi, Yen-Hua Huang, David J. Craik, David J. Adams
Summary: alpha O-Conotoxin GeXIVA, derived from the venom of marine snail Conus generalis, has analgesic activity and acts on GABA(B) receptors, indicating a potential important receptor target for mediating analgesia. The peptide has different structural isomers, with the bead isomer being the most potent at nicotinic acetylcholine receptors.
JOURNAL OF NEUROCHEMISTRY
(2022)
Article
Neurosciences
Mina Peric, Ljiljana Nikolic, Pavle R. Andjus, Danijela Bataveljic
Summary: Amyotrophic lateral sclerosis (ALS) is a fatal neurodegenerative disease characterized by the death of motor neurons, with oligodendrocytes playing an important role in disease progression. Studies in the SOD1(G93A) rat model of ALS show reduced expression of Kir4.1 in spinal cord oligodendrocytes, along with morphological abnormalities indicative of degeneration. Additionally, functional impairment of oligodendrocytes, including decreased Kir currents, suggests a potential role of Kir4.1 channel dysfunction in ALS pathophysiology.
EUROPEAN JOURNAL OF NEUROSCIENCE
(2021)
Article
Microbiology
Elena G. Govorunova, Oleg A. Sineshchekov, Leonid S. Brown, Ana-Nicoleta Bondar, John L. Spudich
Summary: This study investigates the structural motifs of potassium-selective channelrhodopsins (KCRs) and identifies key residues that determine their selectivity. The findings provide a foundation for further improvement of KCRs as optogenetic tools.
Article
Neurosciences
Ezequiel Marron Fernandez de Velasco, Megan E. Tipps, Bushra Haider, Anna Souders, Carolina Aguado, Timothy R. Rose, Baovi N. Vo, Margot C. DeBaker, Rafael Lujan, Kevin Wickman
Summary: This study found that ethanol exposure weakened the somatodendritic inhibitory effect of GABABR activation on principal neurons in the BLA, attributable to suppression of GIRK channel activity and redistribution of GABABR and GIRK channels. Viral suppression of GIRK channel activity replicated some mood-related behaviors observed during ethanol withdrawal.
BIOLOGICAL PSYCHIATRY
(2023)
Article
Physiology
Isabelle Bidaud, Alicia D'Souza, Gabriella Forte, Eleonora Torre, Denis Greuet, Steeve Thirard, Cali Anderson, Antony Chung You Chong, Angelo G. Torrente, Julien Roussel, Kevin Wickman, Mark R. Boyett, Matteo E. Mangoni, Pietro Mesirca
Summary: Genetic ablation of cardiac I-KACh in mice prevents exercise-induced sinus bradycardia by suppressing training-induced remodeling of inward currents I-f, I-CaT and I-CaL. This finding may offer an alternative to pacemaker implantation for the bradyarrhythmias seen in some veteran athletes.
FRONTIERS IN PHYSIOLOGY
(2021)
Article
Anatomy & Morphology
Martin R. Silic, Sarah Haruka Murata, Sung Jun Park, GuangJun Zhang
Summary: Our study identified and renamed 31 kir genes in zebrafish, and analyzed their evolutionary history through phylogenetic and syntenic analyses. The expression of zebrafish kir genes during early embryogenesis showed distinct gene expression domains, indicating subfunctionalization of ohnologous genes from whole-genome duplication. The varied temporal gene expression domains suggest a potential role of Kir channels in embryonic patterning or regulation.
DEVELOPMENTAL DYNAMICS
(2022)
Article
Chemistry, Medicinal
Sion Park, Pia Fluethmann, Carla Wolany, Lena Goedecke, Hannah Maleen Spenner, Thomas Budde, Hans-Christian Pape, Kay Juengling
Summary: The neuropeptide S system, consisting of the NPS and NPSR1, has been extensively studied in rodents. Activation of NPSR1 in amygdalar neurons inhibits voltage-gated potassium channels and increases neuronal excitability through G(alpha q) signaling and calcium ions.
Article
Pharmacology & Pharmacy
Allison Anderson, Baovi N. Vo, Ezequiel Marron Fernandez de Velasco, Corey R. Hopkins, C. David Weaver, Kevin Wickman
Summary: The newly identified GIRK channel inhibitor VU0468554 shows effective inhibition of cardiac GIRK channels, indicating promising therapeutic implications for relevant cardiac arrhythmias. Studies suggest that VU0468554 selectively targets cardiac over neuronal GIRK channels and partially reverses GIRK-mediated bradycardia in isolated mouse heart models.
MOLECULAR PHARMACOLOGY
(2021)
Article
Biochemistry & Molecular Biology
Marcelino Cereijido, Lidia Jimenez, Lorena Hinojosa, Aida Castillo, Jacqueline Martinez-Rendon, Arturo Ponce
Summary: This study reveals a new role for ouabain as a modulator of the expression of voltage-gated potassium channels in epithelial cells, which requires cell-cell contacts.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Cell Biology
Xin-Yi Gu, Bo Jin, Zhi-Dan Qi, Xiao-Feng Yin
Summary: This study demonstrates that changes in miR-142a-3p expression play a role in muscle cell electrophysiology by affecting ion channel currents.
NEURAL REGENERATION RESEARCH
(2022)
Article
Oncology
Brigitte Pelzmann, Ahmed Hatab, Susanne Scheruebel, Sonja Langthaler, Theresa Rienmueller, Armin Sokolowski, Astrid Gorischek, Dieter Platzer, Klaus Zorn-Pauly, Stephan W. Jahn, Thomas Bauernhofer, Wolfgang Schreibmayer
Summary: This article investigates the effects of somatic mutations of the KCNJ3 gene on tumors. The experimental results suggest that certain missense mutations may be involved in the genesis and progression of malignancies.
FRONTIERS IN ONCOLOGY
(2022)
Article
Biology
Benjamin M. Zemel, Alexander A. Nevue, Leonardo E. S. Tavares, Andre Dagostin, Peter V. Lovell, Dezhe Z. Jin, Claudio V. Mello, Henrique von Gersdorff
Summary: This study investigated the excitability of upper motor neurons controlling vocal motor function in zebra finches and found that the key command neurons RAPNs exhibited higher firing rates and ultranarrow spikes. Pharmacological and molecular data revealed that RAPNs had higher expression of Kv3.1 channels. The study provides evidence that songbirds and primates have evolved the use of Kv3.1 in upper motor neurons controlling fast and complex motor skills.
Article
Biochemistry & Molecular Biology
Anna M. Olszewska, Adam K. Sieradzan, Piotr Bednarczyk, Adam Szewczyk, Michal A. Zmijewski
Summary: The study reveals that calcitriol can directly affect the activity of the mitochondrial potassium channel in human astrocytoma cells, suggesting its potential role in regulating mitochondrial function.
CELLULAR & MOLECULAR BIOLOGY LETTERS
(2022)
Article
Biochemistry & Molecular Biology
Xia Li, Romain Helleringer, Lora L. Martucci, Glenn Dallerac, Jose-Manuel Cancela, Micaela Galante
Summary: Cerebral ischemia, which is caused by a reduction or interruption in blood supply to the brain, leads to deprivation of oxygen and glucose. The consequences of cerebral ischemia include loss of metabolic ATP, accumulation of K+ and glutamate in the extracellular space, electrolyte imbalance, and brain edema formation. This study focuses on the neuroprotective role of hypothermia in a mouse cerebellar slice model of ischemia, and found that lowering the temperature delays the increase of K+ and tissue swelling. In addition, hypothermia hinders the morphological changes and membrane depolarizations of Bergmann glial cells, suggesting that it reduces harmful homeostatic changes in cerebellar ischemia.