期刊
TOXICON
卷 108, 期 -, 页码 184-188出版社
PERGAMON-ELSEVIER SCIENCE LTD
DOI: 10.1016/j.toxicon.2015.09.038
关键词
Next generation sequencing; Trinity; Sodium channel toxins; Sea anemone venom
资金
- US National Science Foundation [DEB-1257796]
- Division Of Environmental Biology
- Direct For Biological Sciences [1257796] Funding Source: National Science Foundation
Using a partial transcriptome of the snakelocks anemone (Anemonia sulcata) we identify toxin gene candidates that were incorrectly assembled into several Trinity components. Our approach recovers hidden diversity found within some toxin gene families that would otherwise go undetected when using Trinity, a widely used program for venom-focused transcriptome reconstructions. Unidentified hidden transcripts may significantly impact conclusions made regarding venom composition (or other multicopy conserved genes) when using Trinity or other de novo assembly programs. (C) 2015 Elsevier Ltd. All rights reserved.
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