Article
Acoustics
Jiao Li, Donghong Liu, Tian Ding
Summary: The study investigated the gene regulation at the transcription level in Escherichia coli O157:H7 after ultrasonic stimulation, revealing significant differential expressions of 1217 genes, including up-regulated and down-regulated genes related to crucial biomolecular processes. The most enriched pathways were analyzed in carbohydrate metabolism, energy metabolism, membrane transport, signal transduction, transcription, and translation categories. Additionally, the study found that the citrate cycle genes were down-regulated, affecting carbohydrate decomposition and energy production under ultrasonic stress.
ULTRASONICS SONOCHEMISTRY
(2021)
Article
Food Science & Technology
Zhenbin Bi, Xuezhu Gu, Yeyi Xiao, Yajing Zhou, Wenbin Bao, Shenglong Wu, Haifei Wang
Summary: This study revealed the toxic effects of ZEN on porcine intestinal epithelial cells and the regulatory role of the ISLR2 gene. Overexpression of ISLR2 gene can alleviate the cytotoxic effects of ZEN and decrease the expression of inflammatory factors induced by ZEN. This finding provides potential molecular targets against ZEN toxicity.
Article
Veterinary Sciences
Gustav G. Bruer, Daria Goedecke, Manfred Kietzmann, Jessica Meissner
Summary: The study revealed that florfenicol treatment resulted in increased resistance in E. coli, while combination with antihistamine mepyramine lowered the minimum inhibitory concentration for other bacteria, indicating potential benefits in enhancing antibacterial efficacy and modifying effects on gut microbiota.
VETERINARY SCIENCES
(2021)
Article
Food Science & Technology
Xue Yang, Feng Li, Hangyi Ning, Wei Zhang, Dongyan Niu, Zhuo Shi, Sa Chai, Anshan Shan
Summary: This study screened ZEN-degrading bacterial strains from fecal samples using a zearalenone challenge pig model and investigated their degradation characteristics. The results showed that the ZEN challenge model was an effective method for screening ZEN-degrading bacteria.
Article
Infectious Diseases
Dennis Carhuaricra, Carla G. Duran Gonzales, Carmen L. Rodriguez Cueva, Yennifer Ignacion Leon, Thalia Silvestre Espejo, Geraldine Marcelo Monge, Raul H. Rosadio Alcantara, Nilton Lincopan, Luis Luna Espinoza, Lenin Maturrano Hernandez
Summary: This study investigated the prevalence of the mcr-1 gene in livestock in Peru and found a high occurrence in pig and chicken farms. The genetic diversity of mcr-1-positive strains calls for a coordinated surveillance approach to effectively detect and limit the spread of colistin-resistant bacteria.
Article
Veterinary Sciences
Xiaoru Shi, Luchen Yu, Rufeng Huang, Wenbin Bao, Shenglong Wu, Zhengchang Wu
Summary: Overexpression of the CXCL11 gene significantly reduced the interaction ability of E. coli F18 with IPEC-J2 cells, and methylation modification of the mC-7 site in the promoter region of the CXCL11 gene may influence the susceptibility to E. coli F18.
VETERINARY SCIENCES
(2022)
Article
Multidisciplinary Sciences
Francois Bertaux, Sebastian Sosa-Carrillo, Viktoriia Gross, Achille Fraisse, Chetan Aditya, Mariela Furstenheim, Gregory Batt
Summary: Small-scale, low-cost bioreactors are gaining popularity in quantitative systems and synthetic biology. In this study, the authors present ReacSight, a strategy that enhances bioreactor arrays by connecting them with sensitive measurement devices using low-cost pipetting robots. They demonstrate the capabilities of ReacSight in yeast, including real-time optogenetic control of gene expression, exploration of nutrient scarcity effects on fitness and cellular stress, and dynamic control of a two-strain consortium's composition.
NATURE COMMUNICATIONS
(2022)
Article
Immunology
Zhipeng Cao, Ming Qi, Peng Shang, Hui Zhang, Shah Nawaz, Abdul Ghaffar, Qingxia Wu, Hailong Dong
Summary: In this study, fecal samples of Tibetan piglets from Nyingchi area were analyzed for the presence of Diarrheagenic Escherichia Coli. The isolation rate was found to be 41.3%, with the predominant phylogenetic groups being group A (68.6%) and group B2 (15.7%). The highest detection rates of virulence genes were STb and F107 in E.coli, and fimC and ompA in Tibetan pigs. Drug sensitivity testing revealed high resistance rates to mezlocillin, doxycycline, and gentamicin. This study provides important insights for the prevention and treatment of yellow and white dysentery in Tibetan pigs.
MICROBIAL PATHOGENESIS
(2023)
Article
Food Science & Technology
Paula Llorens Castello, Matteo Antonio Sacco, Isabella Aquila, Juan Carlos Molto Cortes, Cristina Juan Garcia
Summary: This study investigated the presence of estrogenic compounds zearalenone (ZON), zearalanone (ZAN), and their metabolites in alternative biological matrices such as liver samples from chicken, pig, and lamb. The results showed that the samples were contaminated with these compounds at varying levels, indicating exposure to mycotoxins. Continuous analysis and monitoring are necessary to control the presence of these mycotoxins in order to protect animal and human health.
Article
Biotechnology & Applied Microbiology
Matej Hrala, Juraj Bosak, Lenka Micenkova, Jitka Krenova, Matej Lexa, Viktoria Pirkova, Zuzana Tomastikova, Ivana Kolackova, David Smajs
Summary: The study identified three potentially probiotic E. coli strains producing antimicrobial compounds that effectively inhibit pathogenic E. coli strains, showing promising results for preventing infections during the postweaning period of piglets.
APPLIED AND ENVIRONMENTAL MICROBIOLOGY
(2021)
Article
Reproductive Biology
Jingjing Wang, Hairui Tian, Hongyang Liu, Jian Wen, Ruihua Huang, Kang Zou, Liming Hou, Pinghua Li
Summary: Exposure to Zearalenone (ZEA) inhibits the proliferation of porcine germline stem cells and causes multiple cellular damages, which may harm the health and fertility of animals.
Article
Veterinary Sciences
Laura Huber, Gunilla Strom Hallenberg, Kamonwan Lunha, Thongpan Leangapichart, Jatesada Jiwakanon, Rachel A. Hickman, Ulf Magnusson, Marianne Sunde, Josef D. Jarhult, Thomas P. Van Boeckel
Summary: In Thailand, pig production has increased significantly in recent decades to meet growing demand for pork, but the use of antimicrobials has also led to an increase in antimicrobial resistance. The study found that farms using antibiotics had a higher proportion of antimicrobial-resistant E. coli, and medium-scale farms with intensive animal production practices showed higher resistance rates. Farms located closer to drugstores and with a higher disease proportion had drug-resistant Escherichia coli.
FRONTIERS IN VETERINARY SCIENCE
(2021)
Article
Chemistry, Multidisciplinary
Menghua Wang, Katherine S. Ryan
Summary: In this study, two new l-Alanosine-producing strains were discovered through genome mining. These strains lack the aspartate-nitrosuccinate pathway genes found in the original producer and use a unique set of nitrate-nitrite reductases to reduce nitrate instead. The repurposing of enzymes from the nitrogen cycle for specialized metabolite biosynthesis and the potential for the discovery of new nitric-oxide-derived natural products are demonstrated.
JOURNAL OF THE AMERICAN CHEMICAL SOCIETY
(2023)
Article
Microbiology
Wietske Dohmen, Apostolos Liakopoulos, Marc J. M. Bonten, Dik J. Mevius, Dick J. J. Heederik
Summary: A long-term study in 39 Dutch pig farms revealed the presence of ESBL-producing Enterobacterales in both pigs and pig farmers, suggesting the possibility of genetic and horizontal transmission. These findings are crucial for developing targeted intervention strategies to reduce the risk of human exposure to ESBL-producing Enterobacterales.
MICROBIOLOGY SPECTRUM
(2023)
Article
Infectious Diseases
Jie Yang, Gang Xiao, Ning Xiao, Zonghan Jiang, Chao Jiang, Yujuan Li, Wenxin Chen, Hongguang Lin, Zhiliang Sun, Jiyun Li
Summary: The plasmid-mediated tigecycline resistance gene tet(X4) was found in Escherichia coli isolates from chicken and pig farms in Hunan province, China. Among the 257 E. coli samples, six tested positive for tet(X4). These tet(X4)-positive strains showed high resistance to multiple drugs, including tigecycline. Whole-genome sequencing and bioinformatics analysis revealed the genomic locations of tet(X4) and identified the plasmid replicon types carrying this gene.
Article
Agriculture, Dairy & Animal Science
I Taranu, D. Marin, G. C. Pistol, A. Untea, M. Vlassa, M. Filip, M. Gras, C. Rotar, A. C. Anghel
Summary: The aim of this study was to investigate the potential of a diet containing rapeseed meal fermented by Saccharomyces cerevisiae to reduce intestinal inflammation, diarrhoea and oxidative stress in piglets after weaning. The results showed that the fermented rapeseed meal diet had a positive effect on piglet intestinal health, reducing the incidence of diarrhoea and improving antioxidant levels.
JOURNAL OF ANIMAL AND FEED SCIENCES
(2022)
Article
Agronomy
Daniela Eliza Marin, Andrei Cristian Anghel, Cristina Valeria Bulgaru, Iulian Grosu, Gina Cecilia Pistol, Ana Elena Cismileanu, Ionelia Taranu
Summary: In conclusion, the nutritional intervention of using agro-industrial wastes rich in omega-3 PUFA can effectively improve piglets' gut health by reducing proinflammatory cytokines and increasing the expression of junction proteins. This eco-friendly approach can help counteract the negative effects of weaning stress on piglets.
Article
Biochemistry & Molecular Biology
Ionelia Taranu, Gina Cecilia Pistol, Andrei Cristian Anghel, Daniela Marin, Cristina Bulgaru
Summary: The present study investigated the potential of a fermented rapeseed meal extract to reduce medicinal ZnO and found that it could be a valuable alternative to zinc oxide in various applications.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Chemistry, Physical
Daniela Negoescu, Irina Atkinson, Mihaela Gherendi, Daniela C. Culita, Adriana Baran, Simona Petrescu, Bogdan Trica, Diana Pelinescu, Robertina Ionescu, Veronica Bratan, Viorica Parvulescu
Summary: TiO2-activated carbon composites with mesoporous structure were prepared by sol-gel method. The study found that activated carbon and titanium precursor had a significant effect on the crystalline phase of TiO2. The results also showed that the phosphorus content in titanium and activated carbon had an important influence on the adsorption and photocatalytic degradation of Brilliant Blue FCF dye and antibiotic Ciprofloxacin.
JOURNAL OF ALLOYS AND COMPOUNDS
(2023)
Article
Agronomy
Daniela Eliza Marin, Gina Cecilia Pistol, Cristina Valeria Procudin, Ionelia Taranu
Summary: Food and feed safety is a major concern for both animal and human health, as contamination with pathogens can occur frequently. This study investigated the effects of simultaneous contamination with mycotoxins and bacterial toxins on cell viability, oxidative stress, and inflammation. The results showed significant additive/synergistic effects of co-exposure to fungal and bacterial toxins, suggesting that the concomitant contamination with bacteria and mycotoxins could lead to higher toxicity.
Article
Multidisciplinary Sciences
Gina Cecilia Pistol, Daniela Eliza Marin, Valeria Cristina Bulgaru, Andrei Cristian Anghel, Mihaela Saracila, Mihaela Vlassa, Miuta Filip, Ionelia Taranu
Summary: The study evaluated the response of grape seed meal byproduct (GSM) to oxidative stress induced by E. coli lipopolysaccharide (LPS) and dextran sulphate sodium (DSS) in vitro and in vivo. The results showed that GSM possessed antioxidant properties and could counteract the oxidative stress response induced by LPS and DSS, as well as restore the levels of endogenous antioxidant enzymes in the colon and lymph nodes. These beneficial effects were mediated through the Nrf2 signaling pathway.
Article
Food Science & Technology
Iulian Alexandru Grosu, Cristina Valeria Bulgaru, Gina Cecilia Pistol, Ana Cismileanu, Daniela Eliza Marin, Ionelia Taranu
Summary: Pigs are highly sensitive to zearalenone (ZEN) contamination, especially after weaning, and it can have adverse effects on their health. There are no clear regulations regarding the maximum limit of ZEN in piglet feed, so further investigations are needed. This study examines the effects of different concentrations of ZEN on the microbiota, SCFA synthesis, and various markers in the colon of young pigs.
Article
Food Science & Technology
Roua Gabriela Popescu, George Catalin Marinescu, Andreea Luminita Radulescu, Daniela Eliza Marin, Ionelia Taranu, Anca Dinischiotu
Summary: This study investigates the proteomic effects of mycotoxins on piglets' liver and the potential compensatory effects of grape seed and sea buckthorn meal as dietary antioxidants. It found that mycotoxins affect metabolism, antioxidant capacity, and various metabolic pathways in piglets' liver, and antioxidants partially restore the expression of affected proteins. This research is of great significance for protecting the health of farm animals and the quality of meat consumed by humans.
Article
Food Science & Technology
Daniela Eliza Marin, Ionelia Taranu
Summary: In this study, an in silico working model based on online computational programs was used to predict the Phase I and II metabolites of alternariol and provide a detailed ADME/Tox profile. A total of 12 metabolites were identified corresponding to the metabolomic profile of alternariol. The ADME profile indicated moderate or high intestinal absorption probability but low probability to penetrate the blood-brain barrier. The computational model also predicted other toxicological endpoints for the compounds, such as vascular toxicity, haemato-toxicity, diarrhea, and nephrotoxicity.
Article
Agronomy
Valeria Cristina Bulgaru, Ana Maria Pertea, Iulian Alexandru Grosu, Andrei Cristian Anghel, Gina Cecilia Pistol, Daniela Eliza Marin, Anca Dinischiotu, Ionelia Taranu
Summary: Zearalenone (ZEN), a mycotoxin produced by Fusarium fungi, contaminates food and feed, causing health issues for humans and animals. Pigs, as major consumers of grains, are particularly sensitive to mycotoxins. This study observed the effects of ZEN at concentrations below and above the EU recommendation on oxidative and inflammatory responses in the colon of weaned piglets. Exposure to the lower concentration of ZEN (75 ppb) did not result in changes in stress and inflammation markers or associated signaling pathways.
Article
Agriculture, Dairy & Animal Science
Ionelia Taranu, Gina Cecilia Pistol, Mihai Laurentiu Palade, Cristina Valeria Bulgaru, Mihaela Habeanu, Andrei Critian Anghel, Daniela Marin
Summary: Fermented rapeseed meal has positive effects on inflammation, oxidation, and antioxidation in weaned piglets, reducing antinutrients and enriching bioactive compounds, and enhancing antiviral and antibacterial response.
SCIENTIFIC PAPERS-SERIES D-ANIMAL SCIENCE
(2022)
Article
Veterinary Sciences
Mihaela Habeanu, Nicoleta Aurelia Lefter, Anca Gheorghe, Mariana Ropota, Smaranda Mariana Toma, Gina Cecilia Pistol, Ioan Surdu, Mihaela Dumitru
Summary: Hemp seed oil can enrich the diets of sows with essential fatty acids, positively impacting the health of piglets and the environment. Feeding sows with hemp seed oil results in improved immune markers and reduced diarrhea frequency in piglets, as well as decreased enteric methane emission.
VETERINARY SCIENCES
(2022)
Article
Pharmacology & Pharmacy
Mohammadhossein Movassaghghazani, Nazanin Shabansalmani
Summary: The study revealed high levels of AFM1 in both breast milk and powdered milk samples in Tehran, with some samples exceeding the established limits. Therefore, monitoring and regulating toxin levels in these products is crucial for maternal and infant health.
Article
Pharmacology & Pharmacy
Defeng Wen, Wantong Han, Quan Chen, Guanhui Qi, Mengling Gao, Pu Guo, Yu Liu, Zhongyuan Wu, Shulin Fu, Qirong Lu, Yinsheng Qiu
Summary: Luteolin can inhibit FB1-induced intestinal inflammatory injury by inhibiting the activation of NF-kappa B and ERK signaling pathways, according to network pharmacology and in vitro experiments.
