Article
Food Science & Technology
Zhenbin Bi, Xuezhu Gu, Yeyi Xiao, Yajing Zhou, Wenbin Bao, Shenglong Wu, Haifei Wang
Summary: This study revealed the toxic effects of ZEN on porcine intestinal epithelial cells and the regulatory role of the ISLR2 gene. Overexpression of ISLR2 gene can alleviate the cytotoxic effects of ZEN and decrease the expression of inflammatory factors induced by ZEN. This finding provides potential molecular targets against ZEN toxicity.
Article
Veterinary Sciences
Aixin Gu, Lige Yang, Jingjing Wang, Jianping Li, Anshan Shan
Summary: Zearalenone has negative effects on the porcine intestine, but glutamine and alanyl-glutamine can protect the intestinal barrier function against ZEN exposure, alleviating the damage caused by the mycotoxin.
RESEARCH IN VETERINARY SCIENCE
(2021)
Article
Cell Biology
Martin Stollmeier, Stefan Kahlert, Werner Zuschratter, Michael Oster, Klaus Wimmers, Berend Isermann, Hermann-Josef Rothkoetter, Constanze Nossol
Summary: Improved oxygen availability in ALI cultures of small intestinal enterocytes leads to morphological, metabolic, and functional changes. A microarray analysis identified 830 significantly regulated genes in SMC and ALI cultures, with alterations in 31 pathways, mainly metabolic pathways, carbon metabolism, glycolysis, and HIF signaling. HIF-1 alpha showed increased mRNA levels and reduced nuclear content in ALI. Enhanced mRNA levels of oxidative phosphorylation genes were confirmed by western blot analysis, while COX subunit 5B protein was decreased. ALI cultures exhibited higher cytochrome C oxidase activity and decreased glucose levels with reduced lactate production. Energy metabolism analysis showed a shift towards oxidative phosphorylation and suppressed glycolysis in ALI.
HISTOCHEMISTRY AND CELL BIOLOGY
(2023)
Article
Biochemistry & Molecular Biology
Biying Huang, Jingjing Wang, Aixin Gu, Tianhu Wang, Jianping Li, Anshan Shan
Summary: This study investigated the mechanism of ZEN-induced intestinal barrier damage and the involvement of the RhoA/ROCK signaling pathway. The findings showed that ZEN disrupted the intestinal epithelial barrier and activated the RhoA/ROCK signaling pathway.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Cell Biology
Qiao Li, Changning Yu, Yanhong Chen, Shangxi Liu, Paula Azevedo, Joshua Gong, O. Karmin, Chengbo Yang
Summary: Citral has been found to significantly suppress PGN-induced inflammation in the porcine jejunal epithelial cell line, reducing its impact on intestinal nutrient absorption and barrier function possibly through TLR2-mediated activation of the NF-kappa B signaling pathway. Therefore, PGN degradation through enzymes and the inclusion of citral to attenuate inflammation may improve pig gut health and functionality.
JOURNAL OF CELLULAR PHYSIOLOGY
(2022)
Article
Biochemistry & Molecular Biology
Linda Droessler, Valeria Cornelius, Alexander G. Markov, Salah Amasheh
Summary: The study demonstrates the detrimental effects of TNFα on non-transformed intestinal epithelial cells, leading to barrier dysfunction. This is achieved through modulation of tight junction proteins and TNF receptors.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Biochemistry & Molecular Biology
Lei Xu, Yanpin Li, Zixi Wei, Rong Bai, Ge Gao, Wenjuan Sun, Xianren Jiang, Junjun Wang, Xilong Li, Yu Pi
Summary: This study found that CDCA can promote the proliferation of IPEC-J2 cells by regulating cell cycle progression and mitochondrial function.
Article
Biology
Giorgia Maroni, Mahmoud A. Bassal, Indira Krishnan, Chee Wai Fhu, Virginia Savova, Rapolas Zilionis, Valerie A. Maymi, Nicole Pandell, Eva Csizmadia, Junyan Zhang, Barbara Storti, Julio Castano, Riccardo Panella, Jia Li, Corinne E. Gustafson, Sam Fox, Rachel D. Levy, Claire Meyerovitz, Peter J. Tramontozzi, Kimberly Vermilya, Assunta De Rienzo, Stefania Crucitta, Daniela S. Basseres, Marla Weetall, Art Branstrom, Alessandra Giorgetti, Raffaele Ciampi, Marzia Del Re, Romano Danesi, Ranieri Bizzarri, Henry Yang, Olivier Kocher, Allon M. Klein, Robert S. Welner, Raphael Bueno, Maria Cristina Magli, John G. Clohessy, Azhar Ali, Daniel G. Tenen, Elena Levantini
Summary: A tumor-specific, mutant-KRAS-associated subpopulation was identified in human and murine lung cancer, offering promise for targeted therapy developments.
COMMUNICATIONS BIOLOGY
(2021)
Article
Physiology
Xiaoya Zhao, Bingqi Dong, Marissa Friesen, Shangxi Liu, Changqing Zhu, Chengbo Yang
Summary: The study demonstrated that capsaicin can attenuate LPS-induced inflammation response through TLR4/NF-kappa B pathway, and improve barrier integrity and glucose absorption.
FRONTIERS IN PHYSIOLOGY
(2021)
Article
Environmental Sciences
Yihao Huang, Shangkun Mo, Yi Jin, Zhuoning Zheng, Hanyi Wang, Shaojuan Wu, Zhuqing Ren, Jian Wu
Summary: Ammonia can induce apoptosis in intestinal epithelial cells through increased levels of ROS. It also disrupts the intestinal barrier function and affects the expression levels of tight junction molecules. The antioxidant NAC can effectively alleviate the cytotoxicity and apoptosis caused by ammonia.
ECOTOXICOLOGY AND ENVIRONMENTAL SAFETY
(2022)
Article
Cell Biology
Damian Tanski, Agnieszka Skowronska, Malgorzata Tanska, Ewa Lepiarczyk, Mariusz T. Skowronski
Summary: The research shows that estradiol, progesterone, and arachidonic acid regulate the expression of AQP1 and AQP5 in porcine luminal epithelial cells through the PKA and MAPK signaling pathways.
Article
Food Science & Technology
Nisreen Faizo, Chandrakala Aluganti Narasimhulu, Anna Forsman, Shibu Yooseph, Sampath Parthasarathy
Summary: The study utilized RNA sequencing to investigate the impact of exposure to peroxidized linoleic acid on cellular processes, uncovering alterations in lipid metabolic pathways, detoxification mechanisms, and suppression of oxidative phosphorylation. These findings provide insights into the effects of 13-HPODE on cellular processes and propose potential therapeutic strategies for LOOH-related pathologies.
Article
Microbiology
Hannah Marks, Lukasz Grzeskowiak, Beatriz Martinez-Vallespin, Heiko Dietz, Juergen Zentek
Summary: Numerous bioactive plant additives have shown positive effects in pigs and chickens, leading to an increased demand for natural feed additives. The development of reliable cell models, such as the IPEC-J2 cell line for pigs and an intestinal epithelial cell model for chickens, is important for testing phytogenic feed additives. Further studies are needed to standardize the use of these cell lines and explore the potential applications of co-cultivation and organoids.
Article
Microbiology
Jana Gruttner, Jorik M. van Rijn, Petra Geiser, Alexandra Florbrant, Dominic-Luc Webb, Per M. Hellstrom, Magnus Sundbom, Mikael E. Sellin, Staffan G. Svard
Summary: Giardia intestinalis is a parasite that infects the upper small intestine of mammals. It can cause diarrhoeal disease in humans, but many infections do not cause symptoms. The molecular mechanisms behind these different outcomes are still unclear.
Article
Biotechnology & Applied Microbiology
Xiaoxiang Xu, Juan Chang, Ping Wang, Chaoqi Liu, Ting Zhou, Qingqiang Yin, Guorong Yan
Summary: This study investigated the effect of combining glycyrrhinic acid (GA) with Saccharomyces cerevisiae (S. cerevisiae) and Enterococcus faecalis (E. faecalis) on alleviating cell damage induced by deoxynivalenol (DON). The results showed that the optimal combination significantly promoted cell viability and reduced DON toxicity. Additionally, the combination improved intestinal barrier function and nutrient absorption. These findings suggest that the combination of GA, S. cerevisiae, and E. faecalis may have potential as a dietary supplement for individuals exposed to DON-contaminated food.
Article
Agriculture, Dairy & Animal Science
I Taranu, D. Marin, G. C. Pistol, A. Untea, M. Vlassa, M. Filip, M. Gras, C. Rotar, A. C. Anghel
Summary: The aim of this study was to investigate the potential of a diet containing rapeseed meal fermented by Saccharomyces cerevisiae to reduce intestinal inflammation, diarrhoea and oxidative stress in piglets after weaning. The results showed that the fermented rapeseed meal diet had a positive effect on piglet intestinal health, reducing the incidence of diarrhoea and improving antioxidant levels.
JOURNAL OF ANIMAL AND FEED SCIENCES
(2022)
Article
Agronomy
Daniela Eliza Marin, Andrei Cristian Anghel, Cristina Valeria Bulgaru, Iulian Grosu, Gina Cecilia Pistol, Ana Elena Cismileanu, Ionelia Taranu
Summary: In conclusion, the nutritional intervention of using agro-industrial wastes rich in omega-3 PUFA can effectively improve piglets' gut health by reducing proinflammatory cytokines and increasing the expression of junction proteins. This eco-friendly approach can help counteract the negative effects of weaning stress on piglets.
Article
Biochemistry & Molecular Biology
Ionelia Taranu, Gina Cecilia Pistol, Andrei Cristian Anghel, Daniela Marin, Cristina Bulgaru
Summary: The present study investigated the potential of a fermented rapeseed meal extract to reduce medicinal ZnO and found that it could be a valuable alternative to zinc oxide in various applications.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Food Science & Technology
Mihaela Vlassa, Miuta Filip, Ionelia Taranu, Daniela Marin, Arabela Elena Untea, Mariana Ropota, Catalin Dragomir, Mihaela Saracila
Summary: This study aimed to evaluate the changes in bioactive, nutritional and anti-nutritional factors in rapeseed meal fermented with Saccharomyces cerevisiae or Saccharomyces boulardii yeasts at different time periods for improving the nutritional characteristics in piglets' feeding. The results showed that fermentation reduced the content of anti-nutritional factors and increased the protein content of the rapeseed meal without major adverse effects on its overall nutritive value.
Article
Agronomy
Daniela Eliza Marin, Gina Cecilia Pistol, Cristina Valeria Procudin, Ionelia Taranu
Summary: Food and feed safety is a major concern for both animal and human health, as contamination with pathogens can occur frequently. This study investigated the effects of simultaneous contamination with mycotoxins and bacterial toxins on cell viability, oxidative stress, and inflammation. The results showed significant additive/synergistic effects of co-exposure to fungal and bacterial toxins, suggesting that the concomitant contamination with bacteria and mycotoxins could lead to higher toxicity.
Article
Multidisciplinary Sciences
Gina Cecilia Pistol, Daniela Eliza Marin, Valeria Cristina Bulgaru, Andrei Cristian Anghel, Mihaela Saracila, Mihaela Vlassa, Miuta Filip, Ionelia Taranu
Summary: The study evaluated the response of grape seed meal byproduct (GSM) to oxidative stress induced by E. coli lipopolysaccharide (LPS) and dextran sulphate sodium (DSS) in vitro and in vivo. The results showed that GSM possessed antioxidant properties and could counteract the oxidative stress response induced by LPS and DSS, as well as restore the levels of endogenous antioxidant enzymes in the colon and lymph nodes. These beneficial effects were mediated through the Nrf2 signaling pathway.
Article
Food Science & Technology
Iulian Alexandru Grosu, Cristina Valeria Bulgaru, Gina Cecilia Pistol, Ana Cismileanu, Daniela Eliza Marin, Ionelia Taranu
Summary: Pigs are highly sensitive to zearalenone (ZEN) contamination, especially after weaning, and it can have adverse effects on their health. There are no clear regulations regarding the maximum limit of ZEN in piglet feed, so further investigations are needed. This study examines the effects of different concentrations of ZEN on the microbiota, SCFA synthesis, and various markers in the colon of young pigs.
Article
Food Science & Technology
Roua Gabriela Popescu, George Catalin Marinescu, Andreea Luminita Radulescu, Daniela Eliza Marin, Ionelia Taranu, Anca Dinischiotu
Summary: This study investigates the proteomic effects of mycotoxins on piglets' liver and the potential compensatory effects of grape seed and sea buckthorn meal as dietary antioxidants. It found that mycotoxins affect metabolism, antioxidant capacity, and various metabolic pathways in piglets' liver, and antioxidants partially restore the expression of affected proteins. This research is of great significance for protecting the health of farm animals and the quality of meat consumed by humans.
Article
Food Science & Technology
Daniela Eliza Marin, Ionelia Taranu
Summary: In this study, an in silico working model based on online computational programs was used to predict the Phase I and II metabolites of alternariol and provide a detailed ADME/Tox profile. A total of 12 metabolites were identified corresponding to the metabolomic profile of alternariol. The ADME profile indicated moderate or high intestinal absorption probability but low probability to penetrate the blood-brain barrier. The computational model also predicted other toxicological endpoints for the compounds, such as vascular toxicity, haemato-toxicity, diarrhea, and nephrotoxicity.
Article
Agronomy
Valeria Cristina Bulgaru, Ana Maria Pertea, Iulian Alexandru Grosu, Andrei Cristian Anghel, Gina Cecilia Pistol, Daniela Eliza Marin, Anca Dinischiotu, Ionelia Taranu
Summary: Zearalenone (ZEN), a mycotoxin produced by Fusarium fungi, contaminates food and feed, causing health issues for humans and animals. Pigs, as major consumers of grains, are particularly sensitive to mycotoxins. This study observed the effects of ZEN at concentrations below and above the EU recommendation on oxidative and inflammatory responses in the colon of weaned piglets. Exposure to the lower concentration of ZEN (75 ppb) did not result in changes in stress and inflammation markers or associated signaling pathways.
Article
Veterinary Sciences
Mihaela Habeanu, Nicoleta Aurelia Lefter, Anca Gheorghe, Mariana Ropota, Smaranda Mariana Toma, Gina Cecilia Pistol, Ioan Surdu, Mihaela Dumitru
Summary: Hemp seed oil can enrich the diets of sows with essential fatty acids, positively impacting the health of piglets and the environment. Feeding sows with hemp seed oil results in improved immune markers and reduced diarrhea frequency in piglets, as well as decreased enteric methane emission.
VETERINARY SCIENCES
(2022)
