Article
Endocrinology & Metabolism
Ziyi Li, Yafei Wang, Shilun Li, Yukun Li
Summary: Bone regeneration is a complex process involving the coordination of osteogenesis and osteoclastogenesis, with the balance between osteogenesis and adipogenesis of BMSCs playing a critical role. Recent studies have shown that exosomes from M2 macrophages facilitate osteogenesis and reduce adipogenesis through the miR-690/IRS-1/TAZ axis, potentially serving as a therapeutic tool for bone loss diseases.
FRONTIERS IN ENDOCRINOLOGY
(2021)
Review
Cell Biology
Xinyun Xu, Ling Zhao, Paul D. Terry, Jiangang Chen
Summary: Mesenchymal stem cells from bone marrow can differentiate into either adipocytes or osteoblasts, and external stimuli such as environmental contaminants, heavy metals, dietary, and physical factors can influence this fate decision. Maintaining a balance between osteogenesis and adipogenesis is crucial for bone homeostasis, and disruptions in lineage commitment of these stem cells are associated with various bone disorders. This review focuses on understanding how external stimuli affect the fate of mesenchymal stem cells and calls for future studies to investigate the impact on bone health and underlying mechanisms of differentiation.
Article
Cell Biology
Haitao Wang, Peng Wei, Yi Zhang, Yuebai Li, Li Yin
Summary: lncRNA plays a significant role in osteoporosis, regulating osteogenic differentiation, adipogenic differentiation, and osteogenic-angiogenic coupling of bone marrow mesenchymal stem cells through the TCONS_00023297/miR-608/RUNX2/SHH signaling axis.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2021)
Article
Orthopedics
Xiaoxiong Huang, Weikai Chen, Chao Gu, Hao Liu, Mingzhuang Hou, Wanjin Qin, Xuesong Zhu, Xi Chen, Tao Liu, Huilin Yang, Fan He
Summary: This study investigates the regulatory role of melatonin in the differentiation of bone marrow-derived mesenchymal stem cells (BMMSCs) towards osteoblasts or adipocytes. The results show that melatonin activates the SIRT1 signaling pathway and switches the differentiation of BMMSCs from osteogenesis to adipogenesis, preventing bone loss in postmenopausal osteoporosis.
JOURNAL OF ORTHOPAEDIC TRANSLATION
(2023)
Article
Biochemistry & Molecular Biology
Zun Wang, Xiaohua Li, Junxiao Yang, Yun Gong, Huixi Zhang, Xiang Qiu, Ying Liu, Cui Zhou, Yu Chen, Jonathan Greenbaum, Liang Cheng, Yihe Hu, Jie Xie, Xucheng Yang, Yusheng Li, Martin R. Schiller, Yiping Chen, Lijun Tan, Si-Yuan Tang, Hui Shen, Hong-Mei Xiao, Hong-Wen Deng
Summary: This study utilized single-cell RNA sequencing to identify distinct subpopulations of BM-MSCs in humans, shedding light on their cellular heterogeneity and roles in maintaining bone homeostasis, as well as potential novel markers for BM-MSC purification.
INTERNATIONAL JOURNAL OF BIOLOGICAL SCIENCES
(2021)
Article
Cell Biology
Wu Duan, Huajie Zou, Nan Zang, Dongxia Ma, Bo Yang, Lin Zhu
Summary: Bone marrow adipose tissue (MAT) can have both local and systemic effects on metabolic homeostasis. The effects of metformin, a first-line drug for type 2 diabetes, on MAT and bone marrow mesenchymal stem cell (BM-MSC) differentiation are conflicting. In vivo and in vitro experiments showed that metformin increased MAT in mice tibia and promoted both osteogenic and lipogenic gene expression in mouse bone marrow. However, metformin inhibited lipogenesis and promoted osteogenesis of MSC in vitro. The increased MAT may be attributed to the filling of adipose tissue after metformin-induced apoptosis of bone marrow stromal cells.
Article
Biology
Zheting Liao, Yu Jin, Yuhao Chu, Hansen Wu, Xiaoyu Li, Zhonghao Deng, Shuhao Feng, Nachun Chen, Ziheng Luo, Xiaoyong Zheng, Liangxiao Bao, Yongqing Xu, Hongbo Tan, Liang Zhao
Summary: A study using single-cell RNA sequencing explored the transcriptomic landscape of alcohol-induced osteonecrosis of the femoral head (ONFH). The study revealed the relevance of stromal and endothelial cells to the pathogenesis of the disease and provided resources for developing cell type-specific therapeutic strategies.
COMMUNICATIONS BIOLOGY
(2022)
Article
Cell & Tissue Engineering
Feng Zhi, Yi Ding, Rong Wang, Yujiao Yang, Kaiming Luo, Fei Hua
Summary: Exosomal hsa_circ_0006859 is a potential biomarker for postmenopausal osteoporosis and regulates the balance between osteogenesis and adipogenesis in hBMSCs by sponging miR-431-5p.
STEM CELL RESEARCH & THERAPY
(2021)
Article
Cell & Tissue Engineering
Rose Ann G. Franco, Eamonn McKenna, Pamela G. Robey, Ross W. Crawford, Michael R. Doran, Kathryn Futrega
Summary: For the use of bone marrow stromal cells (BMSC) in cartilage repair, controlling their hypertrophic differentiation is crucial. TGF-beta 1 stimulation triggers chondrogenic and hypertrophic differentiation in BMSCs, and silencing SP7, a transcription factor associated with hypertrophic differentiation, reduces both hypertrophic and chondrogenic differentiation processes. However, SP7 silencing compromises overall trilineage differentiation of BMSCs and makes it challenging to promote chondrogenesis while reducing hypertrophy in cartilage tissue engineering.
JOURNAL OF TISSUE ENGINEERING
(2023)
Article
Plant Sciences
Xiaoyan Qin, Qiu Wei, Ran An, Yun Yang, Mingqi Cai, Xiaoling Han, Haoping Mao, Xiumei Gao
Summary: This study investigates the anti-osteoporotic effects of an ethanol extract of FLL in ovariectomized mice and explores the underlying mechanism. The results show that FLL prevents bone loss and improves bone microstructure by modulating bone and fat balance, suggesting its potential as a therapeutic agent for postmenopausal osteoporosis.
PHARMACEUTICAL BIOLOGY
(2023)
Article
Chemistry, Multidisciplinary
Yan Zhang, Chun-Yuan Chen, Yi-Wei Liu, Shan-Shan Rao, Yi-Juan Tan, Yu-Xuan Qian, Kun Xia, Jie Huang, Xi-Xi Liu, Chun-Gu Hong, Hao Yin, Jia Cao, Shi-Kai Feng, Ze-Hui He, You-You Li, Zhong-Wei Luo, Ben Wu, Zi-Qi Yan, Tuan-Hui Chen, Meng-Lu Chen, Yi-Yi Wang, Zhen-Xing Wang, Zheng-Zhao Liu, Ming-Jie Luo, Xiong-Ke Hu, Ling Jin, Teng-Fei Wan, Tao Yue, Si-Yuan Tang, Hui Xie
Summary: This study reveals that osteocytes regulate the differentiation of BMSCs into osteoblasts or adipocytes by secreting neuropeptide Y (NPY), with increased NPY expression associated with aging and osteoporosis. Deletion of NPY in osteocytes leads to high bone mass phenotype and attenuated bone-fat imbalance. The study suggests a new mode of neuronal control of bone metabolism through ANS-induced regulation of osteocyte NPY.
Article
Oncology
Rong Zhou, Fubo Chen, Haixia Liu, Xueqin Zhu, Xueyun Wen, Fang Yu, Guangwei Shang, Shengcai Qi, Yuanzhi Xu
Summary: The study showed that berberine hydrochloride (BBR) can modulate the imbalance between osteogenesis and adipogenesis mediated by Lipopolysaccharide (LPS) from oral pathogenic bacteria, potentially reversing the effects of LPS on gene expression and demonstrating the involvement of AMP-activated protein kinase.
MOLECULAR MEDICINE REPORTS
(2021)
Article
Health Care Sciences & Services
Michael Strug, Lusine Aghajanova
Summary: The uterus is a homeostatic organ that responds to ovarian steroid hormones and prepares the endometrium for embryo implantation. Dysfunction in endometrial repair and regeneration can lead to infertility and abnormal menses. Mesenchymal stem cells have regenerative and immunomodulatory properties and have been investigated for therapeutic purposes in abnormal endometrium.
JOURNAL OF PERSONALIZED MEDICINE
(2021)
Article
Nutrition & Dietetics
Antonio Casado-Diaz, Angel Rodriguez-Ramos, Barbara Torrecillas-Baena, Gabriel Dorado, Jose Manuel Quesada-Gomez, Maria Angeles Galvez-Moreno
Summary: Study found that phloretin can inhibit human adipogenesis by increasing beta-catenin and inducing apoptosis in adipocytes during late differentiation. This suggests the potential of phloretin-rich foods for clinical use and regenerative-medicine applications to combat obesity and prevent bone-marrow adiposity.
Article
Multidisciplinary Sciences
Hyun-Ji Park, Jessica R. Hoffman, Milton E. Brown, Sruti Bheri, Olga Brazhkina, Young Hoon Son, Michael E. Davis
Summary: Small extracellular vesicles (sEVs) play a critical role in cardiac cell therapy by delivering molecular cargo and mediating cellular signaling. Knocking down miR-192-5p and miR-432-5p in cardiac c-kit+ cell-derived sEVs enhances their therapeutic capabilities and improves cardiac function, reducing fibrosis and necrotic inflammatory responses. This study suggests that targeting deleterious miRNAs in sEVs could be a promising therapeutic strategy for chronic myocardial infarction.
Article
Endocrinology & Metabolism
Antonio Casado-Diaz, Gabriel Dorado, Merce Giner, Maria Jose Montoya, Cristina Navarro-Valverde, Adolfo Diez-Perez, Jose Manuel Quesada-Gomez
CALCIFIED TISSUE INTERNATIONAL
(2019)
Review
Cell & Tissue Engineering
Antonio Casado-Diaz, Gabriel Dorado, Jose Manuel Quesada-Gomez
WORLD JOURNAL OF STEM CELLS
(2019)
Review
Biotechnology & Applied Microbiology
Antonio Casado-Diaz, Jose Manuel Quesada-Gomez, Gabriel Dorado
FRONTIERS IN BIOENGINEERING AND BIOTECHNOLOGY
(2020)
Article
Cell Biology
Marta Varela-Eirin, Paula Carpintero-Fernandez, Agustin Sanchez-Temprano, Adrian Varela-Vazquez, Carlos Luis Paino, Antonio Casado-Diaz, Alfonso Calanas Continente, Virginia Mato, Eduardo Fonseca, Mustapha Kandouz, Alfonso Blanco, Jose Ramon Caeiro, Maria D. Mayan
Article
Cell & Tissue Engineering
Marta Camacho-Cardenosa, Jose Manuel Quesada-Gomez, Alba Camacho-Cardenosa, Alejo Leal, Gabriel Dorado, Barbara Torrecillas-Baena, Antonio Casado-Diaz
WORLD JOURNAL OF STEM CELLS
(2020)
Article
Medicine, General & Internal
Merce Giner, Cristina Miranda, Maria Angeles Vazquez-Gamez, Patricia Altea-Manzano, Maria-Jose Miranda, Antonio Casado-Diaz, Ramon Perez-Cano, Maria-Jose Montoya-Garcia
Summary: This study analyzed the impact of type 2 diabetes mellitus on bone quality, finding that patients with T2DM showed poorer trabecular bone structure and mechanical properties.
Article
Nutrition & Dietetics
Antonio Casado-Diaz, Angel Rodriguez-Ramos, Barbara Torrecillas-Baena, Gabriel Dorado, Jose Manuel Quesada-Gomez, Maria Angeles Galvez-Moreno
Summary: Study found that phloretin can inhibit human adipogenesis by increasing beta-catenin and inducing apoptosis in adipocytes during late differentiation. This suggests the potential of phloretin-rich foods for clinical use and regenerative-medicine applications to combat obesity and prevent bone-marrow adiposity.
Article
Pharmacology & Pharmacy
Antonio Casado-Diaz, Jose Manuel Moreno-Rojas, Jose Verdu-Soriano, Jose Luis Lazaro-Martinez, Leocadio Rodriguez-Manas, Isaac Tunez, Manuel La Torre, Miriam Berenguer Perez, Feliciano Priego-Capote, Gema Pereira-Caro
Summary: This study evaluated the antioxidant and wound-healing properties of a novel multifunctional amorphous hydrogel containing Olea europaea leaf extract (OELE). The results showed that the hydrogel had high antioxidant activity and promoted wound healing. Experimental results in human dermal fibroblasts and diabetic mice models confirmed the protective role and wound-healing capacity of the hydrogel.
Article
Medicine, General & Internal
Antonio Casado-Diaz, Manuel La Torre, Feliciano Priego-Capote, Jose Verdu-Soriano, Jose Luis Lazaro-Martinez, Leocadio Rodriguez-Manas, Miriam Berenguer Perez, Isaac Tunez
Summary: The prevalence of chronic wounds is increasing due to population aging and associated pathologies like diabetes. Amorphous hydrogels, such as EHO-85 containing Olea europaea leaf extract, have shown promising potential in enhancing wound healing process by providing a favorable environment for ulcer healing. EHO-85 has demonstrated remarkable moisturizing ability and antioxidant capacity, and it can also modulate the wound microenvironment by affecting reactive oxygen species, pH, and moistening regulation. The study also showed that EHO-85 treatment improved wound healing rates in a murine model of impaired wound healing compared to hydrocolloid dressing.
JOURNAL OF CLINICAL MEDICINE
(2022)
Review
Nutrition & Dietetics
Jose Manuel Quesada-Gomez, Jose Lopez-Miranda, Marta Entrenas-Castillo, Antonio Casado-Diaz, Xavier Nogues y Solans, Jose Luis Mansur, Roger Bouillon
Summary: The COVID-19 pandemic poses a significant challenge to modern medicine and public health systems. The emergence of new variants with increased infectivity and insufficient global vaccination coverage highlight the need for new therapies or the repurposing of existing drugs. This review explores the potential use of calcifediol, a prohormone of the vitamin D endocrine system, for the treatment of COVID-19 and its association with the disease.
Editorial Material
Medicine, General & Internal
Antonio Casado-Diaz
JOURNAL OF CLINICAL MEDICINE
(2022)
Review
Chemistry, Multidisciplinary
Victoria Pulido-Escribano, Barbara Torrecillas-Baena, Gabriel Dorado, Maria angeles Galvez-Moreno, Marta Camacho-Cardenosa, Antonio Casado-Diaz
Summary: Hard-to-heal chronic wounds associated with aging and high-prevalence pathologies can be effectively treated using therapies based on mesenchymal stem cells (MSC) and their secretomes, including extracellular vesicles (EV). These therapies have immunomodulatory activity and can induce angiogenesis, cell proliferation, and cell migration. However, the short half-life of MSC-EV limits their clinical use, and encapsulating them in biomaterials like hydrogels can enhance their regenerative efficiency.
APPLIED SCIENCES-BASEL
(2023)
Article
Medicine, General & Internal
Barbara Torrecillas-Baena, Marta Camacho-Cardenosa, Jose Manuel Quesada-Gomez, Paloma Moreno-Moreno, Gabriel Dorado, Maria Angeles Galvez-Moreno, Antonio Casado-Diaz
Summary: This study aimed to evaluate the effects of sitagliptin, vildagliptin, or 1G244 (a DPP8/9 specific inhibitor) on cell viability, osteogenic and adipogenic differentiation of human MSC. The results showed that the two tested DPP4 inhibitors did not affect MSC viability, but 1G244 significantly decreased it. Additionally, 1G244 and vildagliptin inhibited osteogenesis and adipogenesis, unlike sitagliptin. These findings suggest that DPP8/9 have an important role in the viability and differentiation of human MSC.
JOURNAL OF CLINICAL MEDICINE
(2023)
Review
Medicine, General & Internal
Barbara Torrecillas-Baena, Victoria Pulido-Escribano, Gabriel Dorado, Maria Angeles Galvez-Moreno, Marta Camacho-Cardenosa, Antonio Casado-Diaz
Summary: Bone metabolism is regulated by osteoblasts, osteoclasts, osteocytes, and stem cells. Cell therapy based on mesenchymal stem cells (MSC) has been proposed for the treatment of bone diseases, with the regenerative potential of MSC mediated in part by the secretion of extracellular vesicles (EV). MSC-EV modulate inflammation, angiogenesis, cell proliferation, migration, and differentiation, making them a valuable tool in regenerative medicine.
JOURNAL OF CLINICAL MEDICINE
(2023)
Review
Cell & Tissue Engineering
Barbara Torrecillas-Baena, Maria Angeles Galvez-Moreno, Jose Manuel Quesada-Gomez, Gabriel Dorado, Antonio Casado-Diaz
Summary: DPP4 and its inhibitors are important for diabetes treatment, but their potential effects in regenerative medicine, including on MSC and their differentiation, immunomodulation, and mobilization capacities, should be further explored.
STEM CELL REVIEWS AND REPORTS
(2022)