Article
Biotechnology & Applied Microbiology
Danni Gong, Fei Yu, Meng Zhou, Wei Dong, Dan Yan, Siyi Zhang, Yan Yan, Huijing Wang, Yao Tan, Ying Chen, Bei Feng, Wei Fu, Yao Fu, Yang Lu
Summary: dcECM hydrogels derived from porcine ears through enzymatic digestion show good biocompatibility for delivering chondrocytes and forming subcutaneous cartilage in vivo. The structural and gelation kinetics of the hydrogels vary with ECM concentrations, with 10 mg/ml hydrogels supporting adhesion and proliferation of chondrocytes in vitro. In vivo, 10 mg/ml dcECM hydrogel grafts exhibit similar qualities to native cartilage, indicating their potential for tissue engineering applications in ear cartilage regeneration.
FRONTIERS IN BIOENGINEERING AND BIOTECHNOLOGY
(2021)
Article
Biotechnology & Applied Microbiology
Huiming Jiang, Jun Lu, Jiawei Li, Zizheng Liu, Fufei Chen, Rui Wu, Xingquan Xu, Yuan Liu, Yiqiu Jiang, Dongquan Shi
Summary: A novel acellular cartilage matrix scaffold (ACS) was designed for cartilage regeneration and hyaline-like cartilage formation. The results showed that ACS implantation promoted cartilage repair in full-thickness cartilage defects and osteochondral defects, forming more hyaline-like cartilage at the 6-month follow-up.
Article
Chemistry, Multidisciplinary
Koichiro Hayashi, Toshiki Yanagisawa, Ryo Kishida, Kunio Ishikawa
Summary: This study investigates the effects of scaffold shape on tissue generation. The results show that even tiny protuberances on the scaffold surface can affect the percentage of interscaffold space, thereby exerting dominant effects on osteogenesis.
Review
Chemistry, Multidisciplinary
Raphael J. Crum, Hector Capella-Monsonis, Stephen F. Badylak, George S. Hussey
Summary: Tissue engineering and regenerative medicine focus on developing new techniques, devices, and materials to replace or repair injured or diseased tissue and organs. These approaches use cells, scaffolds, and signaling molecules to promote repair and regeneration. Extracellular vesicles have emerged as key mediators of tissue regeneration and repair.
APPLIED SCIENCES-BASEL
(2022)
Review
Pharmacology & Pharmacy
Zhen Wang, Yichuan Wang, Jiaqi Yan, Keshi Zhang, Feng Lin, Lei Xiang, Lianfu Deng, Zhenpeng Guan, Wenguo Cui, Hongbo Zhang
Summary: Bone regenerative engineering encompasses scaffold design and applications based on electrospinning and 3D bioprinting technologies, aiming to achieve bone tissue regeneration. Electrospun nanofibrous scaffolds and 3D bioprinted scaffolds play crucial roles in bone regeneration, with promising prospects for future development.
ADVANCED DRUG DELIVERY REVIEWS
(2021)
Article
Orthopedics
Andrea Schwab, Marinus A. Wesdorp, Jietao Xu, Florencia Abinzano, Claudia Loebel, Marc Falandt, Riccardo Levato, David Eglin, Roberto Narcisi, Martin J. Stoddart, Jos Malda, Jason A. Burdick, Matteo D'Este, Gerjo J. V. M. Van Osch
Summary: This study investigates the influence of various physicochemical properties of hydrogels on cell migration and related tissue formation using in vitro, ex vivo and in vivo models.
JOURNAL OF ORTHOPAEDIC TRANSLATION
(2023)
Review
Chemistry, Medicinal
Kavitha Ganesan Nathan, Krishnamurithy Genasan, Tunku Kamarul
Summary: Tissue engineering and regenerative medicine (TERM) offers hope for innovative therapies and relies on various strategies. The development of a scaffold, specifically the Polyvinyl alcohol-chitosan (PVA-CS) scaffold, has shown promise in supporting cell growth and tissue regeneration. It can be tailored to fit different tissues and organs and can be combined with other materials and technologies to enhance its regenerative capabilities. This review summarizes the potential role and functions of PVA-CS in TERM applications.
Review
Biotechnology & Applied Microbiology
Afarin Neishabouri, Alireza Soltani Khaboushan, Faezeh Daghigh, Abdol-Mohammad Kajbafzadeh, Masoumeh Majidi Zolbin
Summary: Reproduction of different tissues using scaffolds and materials is an important aspect of regenerative medicine. Decellularized extracellular matrix (dECM) has been used for the goal of regenerating whole organs. Recently, decellularization techniques have been widely utilized in producing suitable scaffolds for damaged organ regeneration. The advantages of decellularized ECM include preserved natural microenvironment features. Different decellularization methods have been developed for specific tissues to remove cells. Evaluation techniques have also been advanced to improve the efficacy of decellularization methods. Modification techniques enhance the characteristics of decellularized scaffolds for damaged tissue regeneration and other applications. However, challenges still exist in the field of decellularization methods and scaffold application.
FRONTIERS IN BIOENGINEERING AND BIOTECHNOLOGY
(2022)
Article
Nanoscience & Nanotechnology
Zhiyao Liao, Liwei Fu, Pinxue Li, Jiang Wu, Xun Yuan, Chao Ning, Zhengang Ding, Xiang Sui, Shuyun Liu, Quanyi Guo
Summary: A bioactive multifunctional scaffold was developed using Mg2+ and aptamer Apt19S to promote articular cartilage regeneration and early inflammatory regulation, offering a promising strategy for AC defect treatment.
ACS APPLIED MATERIALS & INTERFACES
(2023)
Article
Pharmacology & Pharmacy
Valeria Mastrullo, Daan R. van der Veen, Priyanka Gupta, Rolando S. Matos, Jonathan D. Johnston, John H. McVey, Paolo Madeddu, Eirini G. Velliou, Paola Campagnolo
Summary: Pericytes play a critical role in regulating circadian rhythms in endothelial cells and silencing this system disproportionately affects their pro-angiogenic function. Considering the effect of circadian rhythms is important for the development of mature vascular structures and to obtain the maximal reparative effect in tissue engineering and regenerative medicine.
FRONTIERS IN PHARMACOLOGY
(2022)
Review
Biotechnology & Applied Microbiology
Zhanqi Wei, Ganlin Zhang, Qing Cao, Tianhao Zhao, Yixin Bian, Wei Zhu, Xisheng Weng
Summary: Nanomaterials, especially organic nanocomposites, hold great promise for the repair and regeneration of cartilage tissue due to their ability to mimic the microstructure and properties of natural cartilage, enhancing scaffold-cell interactions and improving the functionality of engineered tissue constructs.
BIOENGINEERING-BASEL
(2022)
Article
Biochemistry & Molecular Biology
W. Benton Swanson, Maiko Omi, Seth M. Woodbury, Lindsey M. Douglas, Miranda Eberle, Peter X. Ma, Nan E. Hatch, Yuji Mishina
Summary: This study investigated the impact of scaffold pore curvature on the differentiation trajectory of mesenchymal stem cells (MSCs), demonstrating that the curvature of scaffold pores can influence cell aggregation and differentiation by altering the cytoskeleton, ultimately guiding cell fate specification.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Engineering, Biomedical
Charlot Philips, Lisanne Terrie, Lieven Thorrez
Summary: A review is presented on the progress and challenges in skeletal muscle decellularization, discussing different decellularization methods and applications of the scaffolds. Decellularized skeletal muscle has been primarily studied for regenerative purposes, focusing on tissue-specific morphological features and biochemical cues for muscle regeneration. However, this review expands the potential applications of decellularized skeletal muscle to demonstrate its versatility as a biomaterial.
Review
Pharmacology & Pharmacy
Markel Lafuente-Merchan, Sandra Ruiz-Alonso, Fatima Garcia-Villen, Idoia Gallego, Patricia Galvez-Martin, Laura Saenz-del-Burgo, Jose Luis Pedraz
Summary: This article reviews the advantages of using 3D bioprinting techniques for osteochondral regeneration, discusses the commonly used biomaterials, cell types, and active molecules, and considers the most recent promising results.
Article
Engineering, Biomedical
Cristina Antich, Gema Jimenez, Juan de Vicente, Elena Lopez-Ruiz, Carlos Chocarro-Wrona, Carmen Grinan-Lison, Esmeralda Carrillo, Elvira Montanez, Juan A. Marchal
Summary: The development of a novel biomimetic hydrogel based on decellularized extracellular matrix derived from mesenchymal stem cells for cartilage tissue engineering has shown excellent biocompatibility and the ability to induce chondrogenesis without supplemental factors. This hydrogel also demonstrated the potential to form hyaline cartilage-like tissue after in vivo implantation, highlighting its promise for cartilage repair and regeneration applications.
ADVANCED HEALTHCARE MATERIALS
(2021)
Article
Integrative & Complementary Medicine
Wen-Dien Chang, Hung-Yu Lin, Nai-Jen Chang, Jih-Huah Wu
Summary: The 830 nm LED therapy showed analgesic effects on postexercise DOMS pain, but did not impact muscle repair process. The LED therapy group had significantly higher PPT values at 48, 72, and 96 hours postexercise, with notable differences in joint range of motion at 72 and 96 hours postexercise compared to the placebo group.
EVIDENCE-BASED COMPLEMENTARY AND ALTERNATIVE MEDICINE
(2021)
Article
Urology & Nephrology
Tzu-Ju Chen, Ti-Chun Chan, Chien-Feng Li, Dissanayakage Dilshan Sampath Dissanayaka, Maryam Kianpour, Hong-Lin He, Steven K. Huang, Wan-Shan Li, Nai-Yu Chen, Yow-Ling Shiue
Summary: The study evaluated the expression levels of glycosyltransferase 8 domain containing protein 2 in urothelial carcinoma patients and found that high expression was correlated with poor prognosis. Upregulation of this protein independently predicted worse disease-specific and metastasis-free survival. Additionally, gene ontology enrichment analysis revealed multiple biological processes enriched in relation to this protein's expression.
INTERNATIONAL JOURNAL OF UROLOGY
(2021)
Article
Biology
Tzu-Ju Chen, Seyedeh Zahra Dehghanian, Ti-Chun Chan, Hong-Lin He, Wan-Shan Li, Sina Abdollahi, Nai-Yu Chen, Chien-Feng Li, Yow-Ling Shiue
Summary: Data mining and immunohistochemistry analysis revealed that upregulation of GNB4 protein is associated with poor prognosis in UC patients. High GNB4 expression levels were correlated with clinicopathological features and predicted decreased disease-specific and metastasis-free survival rates. Multiple signaling pathways were enriched in GNB4-related UC progression, emphasizing the significance of GNB4 in UC development.
MEDICAL MOLECULAR MORPHOLOGY
(2021)
Article
Pathology
Pei-Hang Lee, Yu-Chien Kao, Tsung-Han Hsieh, Jia-Bin Liao, Chien-Feng Li, Jen-Chieh Lee, Yi-Ming Chang, Ching-Di Chang, Shih-Chiang Huang, Tzu-Ju Chen, Ting-Ting Liu, Shih-Chen Yu, Hsuan-Ying Huang
Summary: Studying the clinicopathologic and genetic spectrum of extracranial myxoid mesenchymal tumors with fusions between EWSR1/FUS and CREB transcription factors revealed similarities and differences among groups, highlighting the morphologic overlap with myxoid angiomatoid fibrous histiocytoma and myoepithelial tumor/carcinoma. The relationships between fusion types and histology, immunoprofile, and outcome remain unclear.
PATHOLOGY RESEARCH AND PRACTICE
(2022)
Article
Environmental Sciences
Szu-Ying Wu, Yi-Hsun Tsai, Yu-Ting Wang, Wen-Dien Chang, Chia-Lun Lee, Chun-En Aurea Kuo, Nai-Jen Chang
Summary: This study investigates the effects of floss bands (FLOSS) on knee range of motion, balance, single-leg-hop distance, and landing stabilization performance in women. The results show that FLOSS intervention improves hamstring flexibility without impeding static balance, and improves single-leg hop distance and landing stabilization performance for up to 20 minutes. The findings provide valuable insights for physiotherapists or athletic professionals in sports practice settings.
INTERNATIONAL JOURNAL OF ENVIRONMENTAL RESEARCH AND PUBLIC HEALTH
(2022)
Article
Cell Biology
Ti-Chun Chan, Chung-Hsi Hsing, Yow-Ling Shiue, Steven K. Huang, Kun-Lin Hsieh, Yu-Hsuan Kuo, Chien-Feng Li
Summary: This study uncovers the biological significance of the CEBPD/hsa-miR-429/VEGFA axis in angiogenesis and tumor growth in urothelial carcinoma (UC). By inhibiting hsa-miR-429, CEBPD stabilizes the expression of VEGFA, leading to the progression of UC.
Article
Oncology
Hong-Yue Lai, Li-Ching Wu, Po-Hsin Kong, Hsin-Hwa Tsai, Yen-Ta Chen, Yuan-Tso Cheng, Hao-Lun Luo, Chien-Feng Li
Summary: The high prevalence of dialysis and incidence of upper urinary tract urothelial carcinoma (UTUC) in Taiwan may be related to aristolochic acid (AA) exposure. This study developed a fast and cost-effective AA detection platform and found correlations between AA detection and clinical outcomes, suggesting it as a predictive and prognostic biomarker.
FRONTIERS IN ONCOLOGY
(2022)
Article
Oncology
Yu-Wei Hsiao, Jhih-Ying Chi, Chien-Feng Li, Lei-Yi Chen, Yi-Ting Chen, Hsin-Yin Liang, Yu-Chih Lo, Jhen-Yi Hong, Chin-Pin Chuu, Liang-Yi Hung, Jyun-Yi Du, Wen-Chang Chang, Ju-Ming Wang
Summary: Targeting vital protumour factors found in stromal cells in the tumour microenvironment may be an effective strategy in cancer therapy. Loss of PTX3 in stromal cells has been shown to decrease the metastasis and growth of cancer cells. Clinically, stromal PTX3 expression is associated with adverse prognostic features and worse survival outcomes in TNBC. Furthermore, TGF-beta 1 induces PTX3 expression by activating CEBPD in stromal fibroblasts, leading to the metastasis and invasion of TNBC MDA-MB-231 cells.
CLINICAL AND TRANSLATIONAL MEDICINE
(2022)
Review
Biochemistry & Molecular Biology
Meng-Ta Sung, Yeh-Han Wang, Chien-Feng Li
Summary: This review emphasizes the importance of harmonization and standardization of tumor mutational burden (TMB) panels in clinical applications, and summarizes the various factors that affect the estimation of TMB, such as preanalytic, sequencing, bioinformatics, and interpretative factors.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Review
Environmental Sciences
Jeffrey Cayaban Pagaduan, Sheng-Yuan Chang, Nai-Jen Chang
Summary: The purpose of this study is to review the existing literature on the chronic effects of foam rolling (FR) on flexibility and performance. Results revealed conflicting findings regarding the improvement of flexibility through chronic FR, while a majority of the studies reviewed showed no beneficial effects of FR on performance. The effect of FR on recovery remains unclear. These findings suggest the need for further research to establish a consensus on the long-term application of FR in flexibility, recovery, and performance.
INTERNATIONAL JOURNAL OF ENVIRONMENTAL RESEARCH AND PUBLIC HEALTH
(2022)
Article
Oncology
Ting-Ting Liu, Chien-Feng Li, Kien-Thiam Tan, Yi-Hua Jan, Pei-Hang Lee, Chih-Hao Huang, Shih-Chen Yu, Cheng-Feng Tsao, Jui-Chu Wang, Hsuan-Ying Huang
Summary: Genetic aberrations involving DNA damage repair in gastrointestinal stromal tumors (GISTs) have not been well studied. This study characterized DDR abnormalities using sequencing and amplification techniques, and validated the findings using immunofluorescence and immunohistochemistry. The results showed that heterozygous deletions frequently targeted DNA damage-sensing genes and were associated with age, risk levels, and biomarker expression. These findings are important for identifying potential DDR-defective GISTs and assessing prognosis.
Article
Microbiology
Po-Hsin Kong, Cheng-Hsiung Chiang, Ting-Chia Lin, Shu-Chen Kuo, Chien-Feng Li, Chao A. Hsiung, Yow-Ling Shiue, Hung-Yi Chiou, Li-Ching Wu, Hsiao-Hui Tsou
Summary: This study explores a machine learning-based mass spectrometry analysis method for rapid discrimination of methicillin-resistant Staphylococcus aureus (MRSA) in Staphylococcus aureus bacteremia (SAB). By establishing and calibrating the model, this method can identify MRSA 24 hours earlier and adjust the treatment accordingly.
Article
Biology
Kuan-Hua Huang, Ching-Ping Chang, Lan-Hsiang Chien, Chien-Feng Li, Ling-Yu Tang, Yu-Yi Chan, Ting-Feng Wu
Summary: This study aimed to investigate the inhibitory molecular process of pomegranate peel on urinary bladder urothelial carcinoma using a proteomics strategy. The results revealed that pomegranate peel extract can regulate various proteins involved in cell apoptosis, cell proliferation, and signaling pathways, thus inhibiting the growth and spread of cancer cells. These findings suggest that pomegranate might serve as a potential chemopreventive resource for UBUC carcinogenesis.
Article
Rehabilitation
Yi-Syuan Huang, Chia-Lun Lee, Wen-Dien Chang, Nai-Jen Chang
Summary: Ankle sprains are more common in females than in males, and limited ankle dorsiflexion range of motion (DFROM) is a major contributing factor. Tissue flossing with a band has been shown to improve joint range of motion and performance, but its effects on the ankle joint and calf muscle in women are still unclear. This study compared the effectiveness of wrapping the band around the ankle joint versus the calf muscle on exercise performance.
JOURNAL OF BODYWORK AND MOVEMENT THERAPIES
(2023)
Article
Oncology
Kwang-Yu Chang, Nai-Jung Chiang, Shang-Yin Wu, Chia-Jui Yen, Shang-Hung Chen, Yu-Min Yeh, Chien-Feng Li, Xiaoxing Feng, Katherine Wu, Amanda Johnston, John S. Bomalaski, Bor-Wen Wu, Jianjun Gao, Sumit K. Subudhi, Ahmed O. Kaseb, Jorge M. Blando, Shalini S. Yadav, Peter W. Szlosarek, Li-Tzong Chen
Summary: The study showed that the combination of ADI-PEG 20 and pembrolizumab is safe and feasible, but may lead to neutropenia. ADI-PEG 20 treatment increased T cell infiltration in the tumor microenvironment with low PD-L1 expression.
