期刊
OSTEOARTHRITIS AND CARTILAGE
卷 21, 期 7, 页码 990-998出版社
ELSEVIER SCI LTD
DOI: 10.1016/j.joca.2013.04.011
关键词
Cartilage tissue engineering; Osteoarthritis; Pro-inflammatory; Cytokines; Myokines; Stem cells
资金
- NSF [CBET-0966920]
- NIH [R01AR059106, P41 EB002520]
- NIH/NIGMS [K12GM074869]
- Div Of Chem, Bioeng, Env, & Transp Sys
- Directorate For Engineering [0966920] Funding Source: National Science Foundation
Objective: Pro-inflammatory cytokines play an important role in inducing cartilage degradation during osteoarthritis pathogenesis. Muscle is a tissue that lies near cartilage in situ. However, muscle's non-loading biochemical effect on cartilage has been largely unexplored. Here, we tested the hypothesis that muscle cells can regulate the response to pro-inflammatory cytokine-mediated damage in chondrocytes derived from human bone marrow-derived mesenchymal stem cells (hMSCs). Method: hMSCs were allowed to undergo chondrogenic differentiation in porous silk scaffolds in the typical chondrogenic medium for 12 days. For the next 9 days, the cells were cultured in chondrogenic medium containing 50% conditioned medium derived from C2C12 muscle cells or fibroblast control cells, and were subject to treatments of pro-inflammatory cytokines IL-1 beta or TNF alpha. Results: Both IL-1 beta and TNF alpha-induced strong expression of multiple MMPs and hypertrophic markers Runx2 and type X collagen. Strikingly, culturing hMSC-derived chondrocytes in C2C12 muscle cell-conditioned medium strongly inhibited the expression of all these genes, a result further confirmed by GAG content and histological evaluation of matrix protein. To determine whether these effects were due to altered chondrocyte growth and survival, we assayed the expression of cell proliferation marker Ki67, cell cycle arrest markers p21 and p53, and apoptosis marker caspase 3. Muscle cell-conditioned medium promoted proliferation and inhibited apoptosis, thereby suggesting a possible decrease in the cellular aging and death that typically accompanies cartilage inflammation. Conclusion: Our findings suggest the role of muscle in cartilage homeostasis and provide insight into designing strategies for promoting resistance to pro-inflammatory cytokines in hMSC-derived chondrocytes. (C) 2013 Osteoarthritis Research Society International. Published by Elsevier Ltd. All rights reserved.
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